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Current status and suggestions of home pharmacy services under the background of aging
SUN Mengyao, DENG Min, ZHOU Quan, WU Bo,
Accepted Manuscript  doi: 10.12206/j.issn.2097-2024.202208099
Abstract(0) HTML(0) PDF (882KB)(0)
  Objective  To explore how to further improve the quality of home pharmacy services under the background of aging.   Methods  The development history of home pharmacy service in our country in recent years was summarized, and the current status and limitations of home-based pharmacy service were analyzed.   Results  Our country's home-based pharmacy service has gradually matured and standardized from the early stage of independent exploration in various regions, but its quality improvement is still restricted by multiple bottlenecks. It is recommended to increase the popularity of pharmacy services, broaden the promotion channels for rational drug use, and optimize the allocation of pharmacists. etc. to be improved.   Conclusion  It is of great significance to improve the quality of home-based pharmacy services for home-based patients in the community, and it requires the joint efforts of multiple parties to improve it.
Determination and effect of five alkaloids from extracts of Piper longum on rats with experimental myocardial ischemia induced by injection of pituitrin
YI Bowen, LIU Huining, ZHENG Rui, REN Jiawei, LIU Yang
Accepted Manuscript  doi: 10.12206/j.issn.2097-2024.202112011
Abstract(78) HTML(50) PDF (999KB)(1)
  Objective  To determine the content of five alkaloids from extracts of piper longum and test the pharmacodynamic effect of them on rats with experimental myocardial ischemia induced by injection of pituitrin.   Methods  The content of five alkaloids was determined simultaneously by HPLC. The experimental myocardial ischemia in rats was induced by injection of pituitrin, and the absolute value of T wave change and change of heart rate before and after model establishment were chosen to be the observation index. The effects of large, medium and small dose groups were evaluated.   Results  Three batches of samples were analyzed, with the contents of piperine for 56.1%, 49.7%, 51.6%; N-isobutyl-(2E,4E)octadecatrienamide for 4.5%, 4.2%, 4.3%; guineensine for 0.46%, 0.38%, 0.40%; piplartine for 1.73%, 1.67%, 1.70% and piperamide for 0.55%, 0.46%, 0.49%, respectively. All dose groups from extracts of piper longum had significantly reduced the absolute value of T wave and almost have no effect on the change of heart rate, except the high dose group showed the effect of reducing heart rate at some time .  Conclusion  The HPLC method was suitable for the simultaneous determination of five alkaloids from extracts of piper longum. It was shown that extracts of piper longum had good bioactivity in anti-myocardial ischemia.
Research progress of anti-sepsis treatment using traditional Chinese medicine
LING Zhongyi, CHEN Linlin, XING Xinhao, WANG Yan
Accepted Manuscript  doi: 10.12206/j.issn.2097-2024.202204063
Abstract(210) HTML(112) PDF (887KB)(7)
Sepsis is life-threatening with complex pathogenesis. It is a big problem in the medical field. Clinically, antibiotics, hormones and mechanical ventilation are the main treatments. There is a lack of specific therapeutic drugs. The treatment effect is not good. In recent years, more and more progress has been made in the treatment of sepsis with traditional Chinese medicine. This article reviews the etiology, pathogenesis and treatment strategies of sepsis. It focuses on four therapies, including clearing away heat and detoxification, clearing the interior, activating blood circulation and removing blood stasis, and strengthening the foundation. We further discuss the advantages and disadvantages of traditional Chinese medicine in the treatment of sepsis, in order to provide reference for the clinical treatment of sepsis.
Study on material basis of cathepsin K targeted inhibitor in Erzhi Wan
Jiang Yi Ping, Jin Yu E, Zhang Zhi Wei, Xia Tian Shuang, Xu Jia Le, Feng Kun Miao, Xue Li Ming
Accepted Manuscript  doi: 10.12206/j.issn.2097-2024.202202008
Abstract(173) HTML(95) PDF (1145KB)(1)
  Objective  To investigate the active ingredients and components that inhibiting cathepsin K activity in Erzhi Wan, a classic kidney-tonifying formula.   Methods  Then-butanol, dichloromethane, ethyl acetate and petroleum ether parts and 30 active components in Erzhi Wan were screened by established high throughput fluorescence methods of inhibit the binding activity of CTSK with Z-FR-MCA substrate, the formation CTSK with chondroitin sulfate A (CSA) complex activity, and the activity of substrate type I collagen degradation by CTSK. Molecular docking and insoluble collagen substrate binding assays were applied to verify the potential CTSK inhibitors.   Results  The n-butanol and petroleum ether parts of Erzhi Wan inhibited the formation of CTSK CSA* complex by more than 90%, the petroleum ether part inhibited the binding of CTSK to substrate Z-FR-MCA by more than 90%, the collagen degradation inhibition rate of CTSK in n-butanol part was more than 95% and that in petroleum ether part was 58.6%. Among the 30 active components, 11 showed that the inhibition rate of CTSK and CSA* complex formation was more than 50%, and 5 components with the inhibition rate of Z-FR-MCA binding activity more than 50%. Finally, there were four components including eclalbasaponin Ⅸ, (-)-epicatechin gallate, nuezhenoside and wedelolactone. The inhibition rate of collagen degradation was more than 50%. Eclipta saponin IX inhibited the binding rate between collagen fibers and CTSK, up to 60%, but all of them failed to dock with CTSK active site.   Conclusion  There are active components that inhibiting cathepsin K in Erzhi Wan, which mainly exists in the n-butanol ingredients, but the active components is not an active-site inhibitor. It might inhibit the binding of CTSK with oligosaccharides by binding with other sites of CTSK, and then reduce the collagen degradation activity of CTSK.
Bibliometric analysis of brucellosis by CiteSpace
SONG kai, ZHANG yuanlin, SUN rui, GUO mingming, HAN dan, REN lei, SHU lixin
Accepted Manuscript  doi: 10.12206/j.issn.2097-2024.202210013
Abstract(264) HTML(130) PDF (2391KB)(6)
  Objective  To perform a visual bibliometric analysis of hot spots as well as areas of Brucella disease research by searching brucellosis related literature.   Methods  A thematic search was conducted in the web of science core set database using Brucella disease or brucellosis as keywords to retrieve literature between January 1985 and December 2021. CiteSpace was used to visualize the countries, institutions, authors, and keywords in the retrieved relevant literatures.   Results  A total of 4 495 eligible articles studies were included, with the United States being the most populous country and the University of Sao Paulo being the most influential. Jilin University and Shihezi University in Xinjiang, China were relatively influential in this field. For the key words analysis, the top 3 of high-frequency words were infection, diagnosis, abortion, and in the 12 clusters formed, the clusters formed by #1, #4 were larger and the research hotspot continued. Keywords in the last five years including human brucellosis, immunity, zoonotic disease, were highlighted.   Conclusion  Study analysis suggested that human brucellosis with zoonosis was a research hotspot, more scholars focused on the infectious route and other exposure risks of cattle as intermediate hosts, epidemiological studies on brucellosis or will be new trends.
Effect and mechanism of Bajitianwan on preventing D-galactose-induced osteoblast bone loss
XU Weifan, XU Wumu, DING Luying, JIANG Yiping, XIA Tianshuang, XIN Hailiang
Accepted Manuscript  doi: 10.12206/j.issn.2097-2024.202202030
Abstract(176) HTML(97) PDF (1942KB)(3)
[Abstract]Objective To explore the effect and mechanism of Bajitianwan on preventing D-galactose (D-gal)-induced osteoblast bone loss.   Methods  Osteoblasts isolated from 24hold Wistar rats were injured by D-gal and intervened with Bajitianwan extract. The osteoblastic proliferation and differentiation were determined by MTT and alkaline phosphatase (ALP), respectively. The cell reactive oxygen species (ROS) levels were detected by DCFH-DA fluorescent probes. The expression of cellular oxidation-related protein nuclear factor erythroid 2-related factor 2 (Nrf2), phosphorylated protein kinase B (p-AKT), protein kinase B (AKT), heme oxygenase-1 (HO-1), and NADPH quinone oxidoreductase 1 (NQO1) were detected by Western blotting. The intranuclear expression of Nrf2 protein was measured by immunofluorescence.   Results  Bajitianwan extract had significantly increased the osteoblastic proliferation and differentiation, and significantly reduced the intracellular ROS level. Bajitianwan extract had activated the PI3K/AKT pathway via activating the phosphorylation of AKT in osteoblasts, and promoted NQO1 and HO-1 expression. In addition, Bajitianwan had significantly promoted the expression of Nrf2 in the nucleus of osteoblasts, activating Nrf2 signaling pathway, and further promoted bone formation.   Conclusion  This study confirmed that Bajitianwan could prevent D-gal injured osteoblastic bone loss for the first time. The mechanism might be related to the regulation of oxidative stress associated PI3K/AKT and Nrf2 signaling pathway.
Design, synthesis and antifungal activity of novel triazoles containing propyl side chains
ZHANG Haidong, YUAN Fumiao, ZHANG Dazhi, JIANG Yuanying, YU Shichong
Accepted Manuscript  doi: 10.12206/j.issn.2097-2024.202208016
Abstract(268) HTML(124) PDF (932KB)(6)
  Objectives  To study the antifungal activity of a new series of triazole compounds with n-propyl side chain and disubstituted benzyl structure.   Methods  Eleven target compounds were designed and synthesized. The structures were confirmed by 1H NMR, and some compounds were confirmed by 13C NMR or HRMS. Three fungal strains were selected as experimental strains, and the antifungal activity was tested in vitro according to the standardized antifungal sensitivity test method recommended by National Committee for Clinical Laboratory Standards (NCCLS).   Results  Compound B11 showed better activity against Candida albicans SC5314 than fluconazole and was comparable to Posaconazole; Compounds B10 , B11 and B4 showed better activity against Cryptococcus neoformanis H99 than fluconazole, while compounds B2 , B3 , B5 , B6 and B7 showed similar activity to fluconazole against Cryptococcus neoformanis H99; while all compounds showed poor activity against Aspergillus fumigatus.   Conclusions  Some of the target compounds with n-propyl side chain and disubstituted benzyl group structure had certain antifungal activity and could be identified as potential lead antifungal drugs.
Species differences of Baishouwu based on characteristic chromatogram and content determination of acetophenones
LIN Meiyu, CHEN Wenhua, XU Lingchuan, HAN Ting, SU Juan
Accepted Manuscript  doi: 10.12206/j.issn.2097-2024.202207036
Abstract(179) HTML(105) PDF (1627KB)(2)
  Objectives   To provide scientific basis for the quality control of different species of Baishouwu by establishing the HPLC fingerprint of domestic of Baishouwu and determining the main active components of acetophenones.   Methods   HPLC-DAD method was used to determine the HPLC fingerprints of domestic of Baishouwu. Then, the content of 4 kinds of acetophenones in Baishouwu was determined. The column was Diamonsil C18(250mm×4.6mm, 5μm)with the mobile phase of methanol and 0.1% phosphoric acid at a flow rate of 1.0 ml/min. The detection wavelength of p-Hydroxyl acetophenone, baishouwu benzophenone, 2',4'-Dihydroxy acetophenone was set at 260 nm and 2',5'-Dihydroxy acetophenone at 280 nm respectively.   Results  The similarity and cluster analysis in HPLC fingerprint showed that the constituents were significantly different among C. bungei, C. auriculatum and C. wilfordii. The content of total acetophenones in C. wilfordii was significantly higher than that in other localities of C. auriculatum and C. bungei. Conclusions A cetophenone could be used as the evaluation index to evaluate the quality of Baishouwu in different origins. It was found that the chemical composition of three kinds of Baishouwu was significantly different. The content of total acetophenone in C. wilfordii is highest, which could be used as the highest quality resource of Baishouwu.
Progress in preparation and application of biomimetic nano carriers for cell membrane
DAI Yu, WANG Hong Bo, BIAN Kang Qing, GUO Ling Yi, YU Yuan
Accepted Manuscript  doi: 10.12206/j.issn.2097-2024.202202058
Abstract(280) HTML(169) PDF (879KB)(13)
Nanocarriers prepared from organic or inorganic materials are widely used in drug targeting system and diagnosis and treatment of disease. However, there are some problems, such as poor targeting, short circulation time in vivo and improvement in the biocompatibility. Biomimetic nanocarriers has carried out research on the issues, which based on different kinds of cell membrane for the nanocarriers modification, endogenous biofilm improving the biocompatibility of carriers in vivo, more accurate targeting, and even producing immunotherapeutic effect. The principle, method, targeting mechanism and therapeutic effect of biomimetic nano carrier technology of cell membrane have been reviewed in this paper, which provide a new direction for the research of new drug delivery system.
Practice and experience of clinical pharmacists participating in pharmaceutical care for patients after heart valve replacement
LI Ying, QIAN Jiao, ZHU Quangang
Accepted Manuscript  doi: 10.12206/j.issn.2097-2024.202201087
Abstract(252) HTML(109) PDF (855KB)(8)
  Objective  To explore the starting point of pharmaceutical care by clinical pharmacists for patients after heart valve replacement.   Methods  Combined with typical cases, the content, focus and effects of pharmaceutical care for patients after heart valve replacement were summarized.   Results  Clinical pharmacists provided pharmaceutical services such as anticoagulant care and warfarin dosage adjustment, infection monitoring and rational use and care of antibacterial, perioperative airway management, stress ulcer (SU) prevention and pharmaceutical care, and warfarin medication education for patients, which had promoted the rational use of drugs and improved the treatment results of patients.   Conclusion  Pharmaceutical care by clinical pharmacists could be helpful to improve the safety and efficacy of drug treatment for patients after heart valve replacement.
Design, Synthesis and Degradation Activity of BRD4-targeting ATTECs
ZHOU Luozhu, SHENG Chunquan
Accepted Manuscript  doi: 10.12206/j.issn.2097-2024.202206050
Abstract(269) HTML(131) PDF (1372KB)(3)
  Objective   To design and synthesize autophagic degraders targeting BRD4 based on autophagosome tethering compound (ATTEC) strategy and test their BRD4 degradation activity.   Methods   BRD4-targeting ATTECs were constructed by conjugating ispinesib that used as a LC3 ligand and JQ1 through a variety of alkane linkers. The final compounds were confirmed by 1H NMR, 13C NMR and ESI-MS, and their degradation activity in different cell lines were tested by Western Blot.   Results   Five BRD4-ATTEC molecules were successfully synthesized for the first time. Compound No. 4 showed moderate BRD4 degradation activity in different cell lines.   Conclusion   The novel BRD4 autophagic degraders were discovered, which expanded the applicability of targeted autophagic degradation via ATTEC.
Advances in preclinical studies on borneol as an adjuvant for glioma
XIA Jing, CHEN Hang, CHEN Jianming, WU Xin
Accepted Manuscript  doi: 10.12206/j.issn.2097-2024.202203126
Abstract(318) HTML(118) PDF (888KB)(4)
Glioma is a common primary malignant brain tumor. At present, the main clinical treatment is surgical resection combined with radiotherapy and chemotherapy. Due to the selective permeability of the blood-brain barrier and the characteristics of multi-drug resistance of tumor cells, the therapeutic effect is not ideal. In recent years, studies have found that borneol could open the blood-brain barrier and promote the infiltration of chemotherapy drugs. When borneol is combined with or co-carried with chemotherapy drugs, chemotherapy drugs could target more glioma tissues and increase efficacy. The preclinical studies on the combination of borneol and chemotherapy drugs in recent years were reviewed in this article, in order to provide useful reference for the treatment of glioma.
Evaluation of toxin-induced injury and protective effect of triptolide based on a cardiovascular chip model
HE Xiaoli, SHI Yiwei, CHEN Lan, LIU Yue, HONG Zhanying
Accepted Manuscript  doi: 10.12206/j.issn.2097-2024.202206018
Abstract(193) HTML(102) PDF (3324KB)(3)
  Objective  To construct a cardiovascular chip model for evaluating the damage of vascular glycocalyx induced by four marine toxins: okadaic acid (OA), conotoxin (CTX), tetrodotoxin (TTX) and gymnodimine (GYM), and explore the protective effect of triptolide on toxin-induced injury.   Methods  Human umbilical vein endothelial cells(HUVEC) were inoculated into a three-channel microfluidic chip. CCK-8 method and immunofluorescence staining were used to analyze the damage of cell viability and glycocalyx tissue induced by low, middle and high concentrations of marine toxin, as well as the protective effect of triptolide on toxin-induced injury.   Results  The cells in the cardiovascular chip grew well and had structurally intact glycocalyx. Compared with the control group, the activity of HUVEC cells were inhibited in group of the medium and high concentration of OA and high concentration of GYM (P<0.05). The activity of cells had not been inhibited by CTX and TTX significantly , but all the four toxins caused serious damage to the glycocalyx tissue (P<0.01). After pre-protection with triptolide, the toxicity of the four toxins to HUVEC cells and the damage rate of glycocalyx decreased significantly.   Conclusion  The four marine biotoxins could damage the activity and glycocalyx of HUVEC cells in a dose-dependent manner, while triptolide has a protective effect on HUVEC cells injured by toxin.
Study on quality standard of Gardenia jasminoides and its effective parts
GE Wen, GAO Congcong, LI Dongyang, CHEN Weidong, ZHOU Tingting
Accepted Manuscript  doi: 10.12206/j.issn.2097-2024.202210003
Abstract(214) HTML(117) PDF (1069KB)(1)
[Abstract]Objective To study the quality standard of Gardenia jasminoides and its effective parts.   Methods  TLC was used to identify Gardenia jasminoides and its effective parts. The heavy metals, harmful elements, and moisture in Gardenia jasminoides and its effective parts were examined. The content of Gardenia jasminoides and its effective parts was determined by high performance liquid chromatography.   Results  TLC method could be used to identify Gardenia jasminoides and its effective parts. The moisture content of Gardenia jasminoides and its effective parts were 8.4% and 3.2%, respectively. ICP-MS was used to determine the contents of five elements in Gardenia jasminoides and its effective parts simultaneously. There was a good linear relationship between arsenic, cadmium, copper, mercury, and lead in the range of 0~20, 0~10, 0~500, 0~5 and 0~20 ng/ml, respectively; The method detection limit of each metal element was 3.3×10−5~1.3×10−3 mg/kg. The relative standard deviation (RSD) of precision was 0.32%~0.82%. RSD values of each element content showed that the method had good repeatability. And the recoveries of arsenic, cadmium, copper, mercury, and lead were 103%~112%, 98%~99%, 98%~99%, 105%~106% and 100%~103%, respectively (n=3) respectively. The stability of each element was good within 8h. The contents of the five elements were within the limits of the current edition of Chinese Pharmacopoeia. The standard curve equation of gardenia was y=15860x+22543, r=0.9999, indicating that there was a good linear relationship of gardenia in the range of 20.16~322.6 μg/ml. The RSD of precision was 1.86%. RSD of the two samples were 2.38% and 2.60%, respectively, indicated that the method had good repeatability. The average recovery of Gardenia was 99.1% (n=6). The stability of the two solutions was good within 8h. The contents of gardenia and its effective parts were 5.71% and 34.2%, respectively.   Conclusion  The research on the quality of Gardenia jasminoides effective parts was carried out based on the research on the quality of Gardenia jasminoides, and the results met the requirements. Therefore, the method established in this experiment could control the quality of Gardenia jasminoides and its effective parts simultaneously.
Research progress of immunotherapies on correction of immunoparalysis in sepsis
XING XinHao, CHEN LinLin, LING ZhongYi, WANG Yan
Accepted Manuscript  doi: 10.12206/j.issn.2097-2024.202203113
Abstract(293) HTML(132) PDF (940KB)(8)
Immunoparalysis is the main cause of death in patients with intermediate and terminal sepsis. The correction of immunoparalysis is an important direction of sepsis treatment. In the pathological process of sepsis, a variety of factors contribute to the imbalanced secretion of cytokines, weakened function of antigen-presenting cells, apoptosis and depletion of lymphocytes, and ultimately lead to immunoparalysis, secondary infection, and even patient deaths. Cytokines such as GM-CSF, IFN-γ, IL-7, and IL-15, immune checkpoint-related therapies such as PD-1/PD-L1 antibodies, CTLA-4 antibodies, TIM-3 antibodies, and LAG-3 antibodies, and immunoreactive substances such as thymosin α1 and immunoglobulin might be beneficial to correct the immune paralysis of patients. the progress of immunotherapy to correct immune paralysis in sepsis were reviewed in this article.
Research progress on transcription factor ZNF24 in Tumors
Tian Shuo, Li Jianzhong
Accepted Manuscript  doi: 10.12206/j.issn.2097-2024.202204043
Abstract(250) HTML(112) PDF (1182KB)(1)
The transcription factor ZNF24 (also known as ZNF191 or KOX17) is a member of the Krüppel-like zinc finger transcription factor family, with a leucine-rich (Leu) SCAN domain (also known as LeR domain) at the N-terminus and four consecutive typical Kruppel-like zinc finger modities at the C-terminus. ZNF24 is a multifunctional transcription factor involved in the regulation of kinase transcriptional activity, vascular proliferation and development, especially in tumorigenesis and tumor progression. ZNF24 plays an important and complex dual-directional regulation role (promoting and inhibiting) in tumor development, invasion and metastasis by regulating the transcriptional expression of different target genes (such as VEGF, Wnt8B, Twist1, β-catenin and DGL1, etc.) and the competitive binding with protein factors (such as β-catenin). Therefore, elucidating the mechanism of ZNF24 in tumors would provide clues and ideas for the treatment of tumors. The researches status of ZNF24 in tumors were summarized in this paper.
Application of cellular metabolomics in central nervous system diseases
HE Xiaoli, SHI Yiwei, WANG Hui, DAI Jianying, HONG Zhanying
Accepted Manuscript  doi: 10.12206/j.issn.2097-2024.202203006
Abstract(322) HTML(144) PDF (1291KB)(10)
Cell metabolomics is an important branch of metabolomics, which could dynamically monitor cell response and metabolic changes after drugs acting on cells, and look for potential biomarkers. Cell metabolomics has been widely used in illustration of disease mechanism, evaluation of drug efficacy and development of new drug through elucidating the pathophysiological mechanism of the disease and the effect of drug treatment intervention. The researches process of cellular metabolomics and its application in central nervous system diseases were reviewed in order to provide theoretical basis for in-depth study of the pathogenesis and prevention and treatment of central nervous system diseases.
Analysis of medication rules of TCM for perimenopausal syndrome based on literature mining
GAO Min, YANG Yingbo, LIN Li, YING Lingxuan, SUN Lianna, XIAO Wei
Accepted Manuscript  doi: 10.12206/j.issn.2097-2024.202203034
Abstract(251) HTML(112) PDF (1018KB)(3)
  Objective  To investigate the rules of Chinese Traditional Medicine in the treatment of perimenopausal syndrome (PMS) and provide a theoretical basis for the clinical treatment of PMS.   Methods  The literature related to PMS were collected from China Knowledge Network (CNKI), Wanfang database and Weipu database in the past 20 years, the herbal compound prescriptions for the treatment of PMS were screened and a relevant database were established and analyzed by SPSS. Results: The relevant literatures contains 184 Chinese medicine prescriptions/proprietary Chinese medicines, 122 flavors of traditional Chinese medicines, and the drug categories were mainly tonic drugs, antipyretic drugs, astringent drugs, and tranquilizers. The core single-flavor Chinese medicines were Baishao(Radix Paeoniae Alba), Shudihuang(Rehmannia glutinosa ), Danggui(Radix Angelicae Sinensis), Fuling (Indian Buead). The property and flavor covered sweet, bitter, cold, etc.; and the channel tropism belonged to the liver, kidneys, heart, lungs, spleen and meridians. The cluster analysis of high-frequency Chinese Traditional Medicine obtained two main combinations. Conclusion: TCM treatment of PMS focused on replenishing qi, soothing the liver, nourishing the kidneys, nourishing blood and calming the heart, and then according to clinical compatibility with drugs such as soothing the nerves, clearing heat and removing dampness; most of its clinical treatment were Xiaoyaosan, Liuwei Dihuang Pills, and Zhibo Rehmanniae decoction and other prescriptions which were added and subtracted.
Application of Metabonomics in Breast Cancer
MA Xiaoyu, LUO Caiping, LIU Yue
Accepted Manuscript  doi: 10.12206/j.issn.2097-2024.202109112
Abstract(283) HTML(140) PDF (919KB)(7)
Breast cancer is a kind of malignant tumor discovered lately, with a high incidence and a poor prognosis. The shortage of relevant biological biomarkers lead to the unsatisfactory treatment efficacy and the early diagnosis in breast cancer. Metabolomics is a new discipline that uses high-throughput analysis techniques to study the dynamic changes of endogenous metabolites under the influence of different pathological physiological stimulation or gene mutations, which has provided a novel way for biomarker screening and disease diagnosis and treatment. the overview of metabolomics and its applications in breast cancer early diagnosis, drug efficacy evaluation, and disease prognosis were summarized in this review.
Textual Research on the name and reality of edible and medicinal Zisu
LI Chunyan, XU Chen, LU Pengfei, HUANG Baokang
Accepted Manuscript  doi: 10.12206/j.issn.2097-2024.202112055
Abstract(634) HTML(360) PDF (1248KB)(14)
  Objective  To make a textual research on Zisu about the name, origin, morphology and efficacy, a dual-purpose medicinal and edible plant, in order to make the original clear and provide reference basis for clinical application.   Methods  Textual research was carried out from the aspects of synonym, distribution, morphology, efficacy, toxicity and side effects of the plants.   Results  Zisu, Baisu and Su in the herbal medicine of past dynasties belong to the Ren category, which are species or varieties of Perilla frutescens(L.)Britt. Zisu has purple leaves, and its stems, leaves and seeds are used as medicine to mainly lower Qi. While Baisu has green leaves, which could cure wind cold.   Conclusion   There are significant differences in botanical morphology and pharmacological efficacy between Zisu and Baisu, which should be distinguished and used separately. Zisu is a dual-purpose traditional Chinese medicine with a long application history. Baisu is not suitable for edible consumption.
Preparation and anti-Vibrio vulnificus hemolysin A of macrophage membrane hybrid liposome
DAI Yu, GUO Ling Yi, WANG Hong Bo, BIAN Kang Qing, YU Yuan
Accepted Manuscript  doi: 10.12206/j.issn.2097-2024.202207001
Abstract(272) HTML(118) PDF (4583KB)(2)
  Objective  To prepare a biomimetic nano carrier macrophage membrane hybrid liposome by heterozygous macrophage membrane and liposome, which could be used for the clearance and toxicity inhibition of Vibrio vulnificus hemolysin A (vvhA).   Methods  Macrophage membrane was extracted and hybridized with liposome by thin-film evaporation combined extrusion method. The hybridized liposome of macrophage membrane was constructed and characterized. The in vitro detoxification ability of the hybridized vector was evaluated by hemolysis test and cytotoxicity test. The detoxification ability of the vector was evaluated by mouse skin infection model.   Results  Anti toxoid studies in vivo and in vitro showed that the anti-hemolysis rate of macrophage membrane heterozygous liposomes in vitro reached 97.03%, which could effectively inhibit the skin ulceration in subcutaneous infected mice and make the survival rate of abdominal infected mice reach 80%.   Conclusion  The constructed macrophage membrane hybrid liposome had high detoxification ability, which could provide a potential solution and research basis for the prevention and treatment of Vibrio vulnificus infection.
Construction and expression of lentiviral vector overexpressing ZNF24 gene in human colorectal cancer HCT116 cell
TIAN Shuo, LI Jianzhong
Accepted Manuscript  doi: 10.12206/j.issn.2097-2024.202204040
Abstract(330) HTML(215) PDF (1998KB)(6)
  Objective   To establish the human colorectal cancer cell line HCT116 with ZNF24 gene overexpression by constructing lentiviral vector of ZNF24 gene overexpression, and provide material basis for subsequent research.   Methods  The recombinant expression ZNF24 lentiviral plasmid pMT-ZNF24 was constructed by the homologous recombination of ZNF24 gene and 3FLAG tag sequence fragments which was amplified by PCR into lentiviral vector pMT-406.The recombinant plasmid pMT-ZNF24 and the auxiliary packaging vector plasmids pCMV-dR8.9 and pCMV -VSV-G were co-transfected into 293T cells, after which the lentivirus were collected. The virus titer was determined with well dilution method. The lentivirus was transfected into HCT116 cells, and the expression levels of ZNF24 were detected by qRT-PCR and Western blot.   Results  The recombinant vector pMT-ZNF24 was successfully constructed, and the corresponding virus was obtained. The virus titer was 3.25×109 TU / ml. The expression levels of ZNF24 in cells transfected with recombinant ZNF24 lentivirus were significantly higher than those in blank and negative control. Conclusion The ZNF24 gene overexpression lentiviral vector had been constructed , and the corresponding virus and the HCT116 cell line expressing ZNF24 had been obtained .
Mechanism of Shexiang Baoxin Pill in the treatment of atherosclerosis based on network pharmacology
WANG Zhicong, XIE Bin, YUAN Xing, XU Xike, LIU Runhui
Accepted Manuscript  doi: 10.12206/j.issn.2097-2024.202112001
Abstract(386) HTML(142) PDF (2965KB)(11)
[Abstract]Objective: To study the mechanism of Shexiang Baoxin Pill in the treatment of atherosclerosis, and to provide a theoretical basis for long-term clinical application.   Methods  The chemical components and targets of Shexiang Baoxin Pill were collected and screened by TCMSP, TCMID, ETCM and BATMAN databases. The targets related to atherosclerosis were collected and screened by DisGeNet, OMIM, TCMSP, DrugBank and DisGeNet. Drug-compound target network and protein-protein interaction network were constructed. Go and KEGG enrichment analysis of Shexiang Baoxin Pill in the treatment of atherosclerosis were carried out on MetaScape platform.   Results  114 potential therapeutic components of Shexiang Baoxin Pill on atherosclerosis were selected, corresponding to 175 targets. The results of network analysis showed that the main active components of Shexiang Baoxin Pill were chenodeoxycholic acid, ursodeoxycholic acid, cinnamaldehyde and ginsenoside Rb1. The results of pathway enrichment showed that the anti-atherosclerotic mechanism of Shexiang Baoxin Pill was related to the regulation of immunity, inflammation, and metabolism.   Conclusion  The active components of Shexiang Baoxin Pill could act on ALB,INS,AKT1,ACTB,TNF,IL6 and other targets, regulating multiple pathways to achieve the therapeutic effect on atherosclerosis.
Optimization of microwave-assisted extraction of green tea polyphenols by response surface methodology
MA Xiaoyu, LUO Caiping, LIU Yue
Accepted Manuscript  doi: 10.12206/j.issn.2097-2024.202205009
Abstract(339) HTML(161) PDF (2558KB)(7)
  Objective   To optimize the microwave-assisted extraction process of green tea polyphenols.   Methods   The extraction yield of tea polyphenols was figured up by building the standard curve of gallic acid and examining the concentration of tea polyphenols in green tea extract with the introduction of a correction factor. The effects of four single factor levels of microwave extraction time, microwave output power, liquid-to-material yield, and ethanol volume fraction on the extraction yield of tea polyphenols were primarily studied in this experiment. The response surface was applied to further optimize the extraction process of green tea polyphenols after exploring the appropriate range of four single factor levels.   Results   The optimal extraction process was as follows: extraction time 37 s, microwave output power 350 w, material - liquid yield 1:45 (g/ml), ethanol volume fraction 55%, and the actual extraction yield of tea polyphenols was 25.65%, which was not much different from the theoretical value.   Conclusion   The microwave-assisted green tea polyphenol extraction process optimized by response surface methodology is time-saving and practicable, and the extraction yield is high.
Comparison of contents variation of six isoflavones in black beans, semifinished and finished Sojae Semen Praeparatum
JIANG Yuetong, GAO Congcong, ZHANGYujia, OUYangyu, ZHOUTingting
Accepted Manuscript  doi: 10.12206/j.issn.2097-2024.202209085
Abstract(264) HTML(128) PDF (1001KB)(6)
  Objective  To compare the contents variation of six flavonoids includingdaidzin, glycitin, genistin, daidzein, glycitein and genisteinin black beans, semifinished and finished Sojae Semen Praeparatum.Methods The contents of flavonoids were determined by HPLC, the condition were Diamonsil C18 column (4.6 × 250mm,5 μ m) , column temperature 30 ℃, detection wavelength 260nm, mobile phase 0.2% acetic acid water (A) - methanol (B), gradient elution, flow rate 1.0ml/min.  Results  The linearity of this method to determine 6 isoflavones was good (R2≥0.9993) within the determination range, and the recovery rate met the requirements. The RSD of precision, repeatability and stability experiment was less than 4%, 3%and 3%. The results of HPLC showed that the contents of six flavonoidsin Sojae Semen Praeparatum increased significantly compared with black beans. And, the contents of six flavonoids in finished Sojae Semen Praeparatum were slightly more than those in semifinished Sojae Semen Praeparatum.   Conclusion  The HPLC method established in this study could accurately determine the content of 6 isoflavones in Sojae Semen Praeparatum. The content of six isoflavones in black beans could be increased by the fermentation, and the combined isoflavones were transformed into free isoflavones during the fermentation process.
Research progress on new drugs for diabetic nephropathy with potential anti-inflammatory targets
SHI Xiaofei, CHEN Yi, XIANG Kefa, ZHANG Huimin, GAOYue, LIU Xia
Accepted Manuscript  doi: 10.12206/j.issn.2097-2024.202109068
Abstract(257) HTML(130) PDF (898KB)(3)
Diabetic nephropathy (DN) is a common microvascular complication of type 1 diabetes mellitus (T1DM) and type 2 diabetes mellitus (T2DM), which is also the main cause of chronic kidney disease (CKD) and end-stage renal disease (ESRD). However, the treatment methods are limited at present. More and more evidences have indicated that inflammatory response is involved in the pathogenesis and progression of diabetic nephropathy (DN).Several anti-inflammatory strategies that target specific inflammatory mediators (transcription factors, pro-inflammatory cytokines, chemokines, adhesion molecules) and intracellular signaling pathways have shown benefits in the DN rodent model. The mechanisms related to inflammation in the development and progression of DN were summarized and new strategies to prevent or treat DN based on inflammation were briefly discussed in this review.
Dissolution behavior of aripiprazole tablets based on near infrared spectroscopy
WANG Weiqing, YANG Wen, LU Feng
Accepted Manuscript  doi: 10.12206/j.issn.2097-2024.202108066
Abstract(392) HTML(179) PDF (1036KB)(13)
  Objective  To establish a near infrared (NIR) quantitative model for the dissolution behavior of aripiprazole tablets.   Methods  The NIR spectra of aripiprazole tablets were collected and the dissolution tests were performed to determine the dissolution of each tablet at 3, 6, 9, 12, 15 and 30 min. The near infrared spectra regions of 4 000.00-4 396.90, 5 326.43-12 000.00 cm−1 were pretreated by Savitzky-Golay smoothing filter, and the dissolution behavior model was established by partial least squares method.   Results  The root mean square error of calibration (RMSEC) and the root mean square error of prediction (RMSEP) at different time points were analyzed. RMSEP was lower than 8%. The calibration correlation coefficient (RC) and the prediction correlation coefficient (RP) at different time points were above 0.95 (except for the point of 6 min). There was a good correlation between the NIR spectrum and the dissolution at each time point.   Conclusion  NIR spectroscopy could predict the dissolution behavior of aripiprazole tablets, which lays a foundation for online quality monitoring of tablets by NIR spectroscopy.
Research progress of tradition Chinese medicine treatment mechanism of systemic lupus erythematosus based on biomarkers
LIU Yaoyang, WU Xin, ZHOU Lin, ZHAO Yin, XU Huji
Accepted Manuscript  doi: 10.12206/j.issn.2097-2024.202206131
Abstract(330) HTML(157) PDF (909KB)(12)
Systemic lupus erythematosus (SLE) is an autoimmune disease with multiple organ involvement. There are still many limitations and individual differences in the treatment based on glucocorticoids and immunosuppressants. In recent years, more and more studies have shown that the combination of traditional Chinese medicine in the treatment of SLE has the advantages of good efficacy, low adverse reactions, and high safety. However, the exact regulatory mechanism and combined traditional Chinese medicine in the treatment of SLE are still unclear. This paper reviews the research on the mechanism of traditional Chinese medicine in the treatment of SLE from metabonomic, immune cells, lymphocyte factors and apoptosis, etc, provides ideas for exploring the mechanism of traditional Chinese medicine in the treatment of SLE with modern methods.
Therapeutic effects of estrogen-intervened EPCs transplantation on diabetic ischemic stroke rats
DONG Yafen, WANG Jian, Chen Ye, LI Shushu, LIU Helong, QIU Yan
Accepted Manuscript  doi: 10.12206/j.issn.1006-0111.202111111
Abstract(480) HTML(233) PDF (1891KB)(5)
  Objective   To explore the therapeutic effects of estrogen-intervened endothelial progenitor cells( EPCs) transplantation on diabetic ischemic stroke rats.   Methods  PKH26-labeled diabetic EPCs and estrogen-intervened diabetic EPCs were injected into rats via the tail vein 24 h after cerebral ischemia. Cerebral ischemic volume, behavioral changes, ischemic site vascularization and homing of EPCs were measured 3 d after EPCs injection.   Results  Compared with diabetic ischemic rats, estrogen-intervened EPCs transplantation had reduced infarct volumes, improved behavioral scores and ischemic site revascularization and promoted homing of EPCs to sites of injury(P<0.05).   Conclusion  Estrogen-intervened EPCs transplantation had a better therapeutic effect on diabetic ischemic stroke by promoting EPCs homing to injury site and EPCs-medicated neovascularization .
Meta-analysis of the efficacy and safety of Ilaprazole Enteric-Coated Tablets on Reflux esophagitis
CHEN Xiaoyun, ZHAO Xu, ZENG Weiwei
Accepted Manuscript  doi: 10.12206/j.issn.1006-0111.202111080
Abstract(901) HTML(739) PDF (1233KB)(21)
  Objective  To evaluate the efficacy and safety of Ilaprazole Enteric-Coated Tablets in the treatment of RE.   Methods  The databases of CNKI, VIP, Wanfang Data, PubMed, Embase, and The Cochrane Library were searched to collect all the randomized controlled trials (RCTs) of Ilaprazole in the treatment of RE published before April 2021. After data extraction and quality evaluation, the RCTs meeting the inclusion criteria were performed, and the meta-analysis was conducted by RevMan 5.4.   Results  Nine RCTs were included, with a total of 1115 patients of RE. The results of the meta-analysis showed that Ilaprazole Enteric-Coated Tablets were comparable to Esomeprazole Enteric-Coated Tablets in both endoscopic efficiency (90.08% vs. 90.00%, P > 0.05) and symptom relief rates (91.79% vs. 91.23%, P > 0.05), and there was no statistically significant difference in the incidence of adverse reactions (7.99% vs. 8.66%, P > 0.05).   Conclusion  Ilaprazole Enteric-Coated Tablets with lower doses were comparable to Esomeprazole Enteric-Coated Tablets which showed good efficacy and safety in the treatment of reflux esophagitis.
Application progress of ROS-responsive liposomes in anti-tumor research
GAO Xiqing, LU Guangzhao, LU Ying, ZOU Hao
Accepted Manuscript  doi: 10.12206/j.issn.1006-0111.202105086
Abstract(634) HTML(392) PDF (859KB)(20)
[Abstract]Reactive oxygen species(ROS) responsive liposomes are prepared based on the high level of ROS expression in the tumor microenvironment, enabling precise drug delivery to the tumor site. With the addition of photosensitizer, the controllability of drugs in liposomes can be further enhanced.
Cordyceps sinensis extract protects against the ovariectomy-induced bone loss via the action on osteoclasts
PENG Weibiao, WANG Ting, FENG Xu, WANG Jiachun, ZHANG Zhenzhen, CHU Zhiyong
Accepted Manuscript  doi: 10.12206/j.issn.1006-0111.202105037
Abstract(678) HTML(373) PDF (8754KB)(27)
  Objective  To explore the effects of Cordyceps sinensis extract (CSE) on osteoporosis and RANKL-mediated osteoclastogenesis.   Methods  Bone marrow-derived macrophages (BMMs) was isolated from the Bone marrow of C57BL/6 mice. CSE was added in osteoclast differentiation. Osteoclasts were stained by tartrate-resistant acid phosphatase (TRAP). The nearly mature osteoclasts were planted on hydroxyapatite plates and the area of bone lacunae was observed by microscope. The F-actin belt was stained by DAPI and phylloeptide and the number of nuclei was observed by confocal microscopy. The expressions of DC-STAMP, ATP6V0D2, TRAP, CTSK, and NFATC1 were detected by q-PCR. The protein expression of the MAPK pathway was detected by Western Blot. The in vivo experiments were carried out by administering CSE to the ovariectomized mice daily through gavage. After 6 weeks of intervention, mouse femurs were taken for morphological analysis. Peripheral blood was taken for ELISA.   Results  CSE represses osteoclastogenesis, bone resorption, F-actin belts formation, osteoclast specific gene expressions and MAPK signaling pathways in vitro. In vivo study indicated that CSE prevents OVX-induced osteoporosis and preserves bone volume by repressing osteoclast activity and function. It also increases the serum ALP, BGP content, and reduces TRAP content.   Conclusion  CSE can attenuate osteoclast formation and OVX-induced osteoporosis, suggesting potential clinical therapeutic effects for osteoporosis.
Determination and effect of five alkaloids from extracts of Piper longum on rats with experimental myocardial ischemia induced by injection of pituitrin
YI Bowen, LIU Huining, ZHENG Rui, REN Jiawei, LIU Yang
Accepted Manuscript  doi: 10.12206/j.issn.1006-0111.202112011
Abstract(592) HTML(363) PDF (1057KB)(3)
  Objective  To determine the content of five alkaloids from extracts of piper longum and test the pharmacodynamic effect of them on rats with experimental myocardial ischemia induced by injection of pituitrin.   Methods  The content of five alkaloids was determined simultaneously by HPLC. The experimental myocardial ischemia in rats was induced by injection of pituitrin, and the absolute value of T wave change and change of heart rate before and after model establishment were chosen to be the observation index. The effects of large, medium and small dose groups were evaluated.   Results  Three batches of samples were analyzed, with the contents of piperine for 56.1%, 49.7%, 51.6%; N-isobutyl-(2E,4E)octadecatrienamide for 4.5%, 4.2%, 4.3%; guineensine for 0.46%, 0.38%, 0.40%; piplartine for 1.73%, 1.67%, 1.70% and piperamide for 0.55%, 0.46%, 0.49%, respectively. All dose groups from extracts of piper longum had significantly reduced the absolute value of T wave and almost have no effect on the change of heart rate, except the high dose group showed the effect of reducing heart rate at some time .  Conclusion  The HPLC method was suitable for the simultaneous determination of five alkaloids from extracts of piper longum. It was shown that extracts of piper longum had good bioactivity in anti-myocardial ischemia.
Determination of total polyphenols and catechins in betel nut polyphenol extract
MA Jianghong, WANG Rong, DU Xing, ZHAO Anpeng, WANG Zihan, ZHANG Haobo
Accepted Manuscript  doi: 10.12206/j.issn.1006-0111.202106013
Abstract(818) HTML(515) PDF (1036KB)(10)
  Objective   To establish a method for the determination of total polyphenols and catechins in betel nut polyphenols extract, and provide reference for the quality control of betel nut polyphenols extract.   Methods   The content of total phenol in betel nut extract was determined by ultraviolet spectrophotometry. The content of catechins was determined by HPLC.   Results   The linear range of total polyphenols in betel nuts extract was 9.8~58.8 μg/ml. The three components of catechin, epicatechin and protocatechuic acid were completely separated by HPLC, and the linear relationship was good in their respective ranges, with the recoveries between 99.17% and 101.67%, the RSD between 1.2% and 2.5%.   Conclusion   The established method is simple, stable and reliable, which could be used for the quantitative analysis of betel polyphenol extract, and provide experimental basis for the quality control of betel polyphenol extract.
Advances in methodologies for preparation and analysis of new biochromatic stationary phase
CHAI Xinyi, GU Yanqiu, CHEN Xiaofei, CHAI Yifeng
In Press  doi: 10.12206/j.issn.1006-0111.202112087
Abstract(889) HTML(611) PDF (1155KB)(21)
Biochromatography is a new chromatographic technology with great development potential. It has been widely used in drug screening and biomolecular interaction analysis. The core of this technology is the chromatographic stationary phase of biomolecules. Nowadays, it mainly develops cell membrane chromatography, artificial biomimetic membrane chromatography and the various immobilization strategies to directly immobilizes proteins on the stationary phase carrier. This paper reviews the research progress of new biochromatographic stationary phase and the application of biochromatographic analysis based on new stationary phase. And, the applications of biochromatographic stationary phase and micro biochromatographic analysis system based on monolithic column are prospected.
Quality standard improvement study of ginkgo semen decoction pieces
TU Yan, DONG Zhiying, HUANG Mengqiu, TAN Jingfu, CHEN Weixu, SUN Lianna
In Press  doi: 10.12206/j.issn.1006-0111.202103007
Abstract(2176) HTML(766) PDF (929KB)(16)
  Objective   To establish and improve the quality standard of ginkgo semen decoction pieces.   Methods   The morphological character for 29 batches of ginkgo gemen and 12 batches of stir-fried ginkgo gemen were observed, and the moisture contents were assayed using the method in Chinese Pharmacopoeia 2015 edition, the first supplement.   Results   The character of ginkgo gemen and stir-fried ginkgo gemen were consistent in different batches. The moisture content of ginkgo gemen was 8.8% to 12.2%, with an average of 10.5%. The moisture content of stir-fried ginkgo gemen was from 5.4% to 12.3%, with an average of 8.9%. Considering that ginkgo semen decoction pieces are stored for a long time, they are prone to the attack of mildew and insects, and the moisture limit is set to be no more than 10.0% in ginkgo gemen and stir-fried ginkgo.   Conclusion   Compared with the Chinese Pharmacopoeia 2015 edition, the first supplement, the character identification for ginkgo semen decoction pieces was added and the quality standards were improved. The moisture content of ginkgo semen decoction pieces needs to be strictly controlled under 10.0% to prevent mildew and insects.
Safety pharmacological study of the compound of diphenhydramine hydrochloride and caffeine
REN Lijun, GU Jing, SHI Wenjing, WANG Ruina, ZHANG Xiaofang, CHEN Jikuai
Accepted Manuscript  doi: 10.12206/j.issn.1006-0111.202105115
Abstract(946) HTML(657) PDF (849KB)(11)
  Objective   To provide the reference resource for the safe clinical use of the compound of diphenhydramine hydrochloride and caffeine by observing its effects on the nervous system, cardiovascular system and respiratory system in experimental animals.   Methods   Single dose of the compound of diphenhydramine hydrochloride and caffeine was given to animals orally. The effects on climbing ability of mice and blood pressure, electrocardiogram, respiration rate and amplitude in beagle dogs were observed and recorded.   Results   With the dosage of the compound of diphenhydramine hydrochloride and caffeine (diphenhydramine hydrochloride / caffeine ratio is 1/2.4) at 51, 102, 204 mg/kg, there was no significant effect on the climbing ability in mice. With the dosages of 14.2, 28.3, 56.6 mg/kg for male Beagle dogs and 5.66, 14.2, 28.3 mg/kg for female Beagle dogs, no significant effects were observed in systolic blood pressure, diastolic blood pressure, mean arterial pressure, heart rate, ECG(P wave, R wave , T wave, QRS time, PR interval, QT interval), respiratory rate and amplitude.   Conclusion   Under the experimental conditions, single oral dose of the compound of diphenhydramine hydrochloride and caffeine has no significant effect on the nervous system, cardiovascular system and respiratory system in experimental animals. Those results suggest that the compound of diphenhydramine hydrochloride and caffeine is a safe agent for clinical use.
Characterization and function of short-chain dehydrogenases/reductases in hydroxysafflower yellow A biosynthesis pathway
WANG Lunuan, WU Jianhui, HE Beixuan, ZHANG Yanjie, GUO Meili
In Press  doi: 10.12206/j.issn.1006-0111.202201061
Abstract(899) HTML(540) PDF (2417KB)(13)
Objective To explore the function of short-chain dehydrogenases/reductases (SDRs) in safflower flavonoid, especially hydroxysafflower yellow A (HSYA) biosynthesis.   Methods  SDRs involved in HSYA biosynthesis pathway were screened based on safflower transcriptome database and metabolome database. The expression pattern was analyzed by qRT-PCR. The overexpression vector was constructed by seamless cloning technology, then genetically transformed to the Yunnan Weishan safflower strain by Agrobacterium gv3101. The transgenic T2 generation plants were positively verified, and the gene expression of corolla SDRs was analyzed. The content of secondary metabolites was assayed by UPLC-Q-TOF/MS.   Results  Three SDRs genes named CtSDR1, CtSDR2 and CtSDR3 involved in HSYA biosynthesis pathway were screened. Their expression in safflower from high to low was corolla > leaf > stem > root. The expression level in corolla increased gradually with corolla development. qRT-PCR analysis of corolla with positive verification of genome insertion sequence showed that the transcription level of CtSDR3 in corolla of T2 positive plants increased by 2~3 times compared with the blank control group, and the content of secondary metabolite HSYA increased by 7.1%~16.6% (P< 0.05).   Conclusion  CtSDR3 may be involved in the biosynthesis of flavonoids, especially HSYA, in safflower. It provides the support data for explaining the function of CtSDR3 in HSYA biosynthesis pathway.
Effect of traditional Chinese medicine Syngnathus on D-galactose-induced learning and memory impairment in aging mice
ZHANG Jingwen, HE Xuhui, XIA Tianshuang, JIANG Yiping, XIN Hailiang
Accepted Manuscript
Abstract(1078) HTML(561) PDF (2426KB)(8)
  Objective  To study the effect of traditional Chinese medicine, Syngnathus on learning and memory impairment induced by D-galactose in aging mice and its mechanism of action.  Methods  HPLC was used to determine the content of DHA, the active ingredient in anti-learning and memory impairment in Syngnathus. The aging mouse model was prepared by intraperitoneal injection of D-galactose (D-gal). Morris water maze test and Western blot were used to detect the ability of learning and memory, biochemical indicators and protein expression related to oxidative damage in the hippocampus, and to explore the protective effect and mechanism of Syngnathus on learning and memory impairment in aging mice.  Results  HPLC results showed that the DHA content in Syngnathus was 7.7613 mg/g (calculated as crude drug), accounting for about 47% of the total composition. Morris water maze results showed that Syngnathus could reduce the escape latency of learning and memory-impaired aging mice and increase the target quadrant swimming time, the proportion of swimming distance and the number of times of crossing the platform, and improve the learning and memory impairment of mice. In addition, Syngnathus can activate the AKT/FOXO1/SOD2 signaling pathway in the hippocampus of aging mice with learning and memory impairment, promote the expression of oxidative stress pathway-related proteins, and improve the learning and memory impairment in aging mice by reducing the degree of oxidative damage in the hippocampus of aging mice.  Conclusion  This study found that Syngnathus is rich in DHA, which has the effect of improving learning and memory impairment induced by D-galactose in aging mice, and preliminarily clarified that its mechanism of action is related to anti-oxidation. Experimental evidence is provided.
Interaction between cyclosporine A and voriconazole in patients with allogeneic hematopoietic stem cell transplantation
WANG Yani, WU Di, RAO Zhi, LI Maoxing, XI Rui, REN Jun
Accepted Manuscript  doi: 10.12206/j.issn.1006-0111.202111056
Abstract(772) HTML(447) PDF (1228KB)(4)
  Objective  To analyze the drug-drug interaction (DDI) between intravenous voriconazole (VRZ) and intravenous cyclosporine (CsA) in patients after allogeneic hematopoietic stem cell transplantation (Allo-HSCT) and provide an individualized and accurate clinical drug delivery.   Methods  In a self-contrast study, Allo-HSCT patients from January 2019 to December 2019 were enrolled according to the inclusion and exclusion criteria. These patients were treated with CsA and VRZ successively and the blood concentration of CsA and VRZ before and after 5~7 days of VRZ administration were determined with LC-MS/MS. The correlation between the concentration of VRZ and concentration/dose (C/D) ratio of CsA was analyzed with SPSS20.0.   Results  A total of 15 patients with ALLo-HSCT were enrolled. Wilcoxon sign rank sum test was used to compare the change of median C/D of CsA before and after VRZ administration, which had shown significant difference (P<0.001). Spearman correlation analysis was conducted on the increase of C/D ratio between VRZ and CsA, which had no significant correlation between them (ρ=−0.273, P=0.32).   Conclusions  There was obvious drug-drug interaction (DDI) between CsA and VRZ. VRZ increased CsA blood concentration significantly, but there was no significant correlation between VRZ blood concentration and the degree of concentration increase, which might be related to individual difference.
Effects of endophytic fungus SH09 on plant growth and accumulation of active components in Salvia miltiorrhiza
WU Sijia, XIE Xingguang, YANG Yang, ZHENG Chengjian, HAN Ting
Accepted Manuscript  doi: 10.12206/j.issn.1006-0111.202108055
Abstract(922) HTML(578) PDF (1310KB)(15)
  Objective  To study the effects of endophytic fungus Epichloë bromicola SH09 on the plant growth and accumulation of active components in Salvia miltiorrhiza, and improve the quality of medicinal plant S. miltiorrhiza.   Methods  E. bromicola SH09 solid bacterial fertilizer was prepared and co-cultured with S. miltiorrhiza for 60 d and 120 d. Four morphological indexes, fresh weight of roots, dry weight of roots, and the contents of four tanshinones and two phenolic acids in the roots of S. miltiorrhiza from treated group and control group were assayed, respectively.   Results  After 60 d and 120 d co-culture, E. Bromicola SH09 significantly increased the tiller number, plant height, leaf number, leaf area, fresh weight of roots, dry weight of roots, and the content of tanshinones and phenolic acids in S. miltiorrhiz.   Conclusion  The endophytic fungus E. bromicola SH09 can effectively promote the plant growth and improve the accumulation of active components in S. miltiorrhiza, which not only broadens the new ecological functions of endophytic fungi, but also improves the quality of medicinal plant S. miltiorrhiza.
Research progress of STAT3 on liver disease
LI Tingting, ZHANG Junping
Accepted Manuscript  doi: 10.12206/j.issn.1006-0111.202109072
Abstract(721) HTML(640) PDF (851KB)(12)
Signal transduction and transcriptional activator 3 (STAT3) is an important transcription factor that can be activated by many cytokines and growth factors. STAT3 plays a key role in cell growth, proliferation, and differentiation. It has been shown that hyperactivation of STAT3 exists in almost all animal models of liver injury and human liver diseases. Therefore, inhibition of STAT3 activation might become a promising strategy for prevention and treatment of acute liver injury and liver fibrosis. The research progress of STAT3 on liver injury, hepatitis, liver regeneration, liver fibrosis, and hepatocellular carcinoma were mainly discussed in this review.
Long-term protective effects of the nicotiflorin on ischemic stroke rats
YAN Xiaodong, WANG Yeqing, LI Bingfeng, GUO Meili
Accepted Manuscript  doi: 10.12206/j.issn.1006-0111.202201080
Abstract(774) HTML(534) PDF (1207KB)(14)
  Objective  To investigate the long-term protective effects of the nicotiflorin on ischemic stroke rats.   Methods   Ischemic stroke model in rats was established for this study. The effects of nicotiflorin on long-term survival rate, nervous system function, body weight and brain neurons in rats were observed.   Results  The nicotiflorin had significantly improved the long-term survival rate of cerebral ischemia rats, which also promoted weight gain, alleviated pathological damage of brain tissue, maintained morphology of brain neurons and function of nervous system.   Conclusion  The nicotiflorin has obvious long-term protective effect on ischemic stroke rats and the mechanism may be related to the protection of the structure and function of brain neurons.
Overview of surface-enhanced Raman spectroscopy in combination with other detection techniques
CUI Xiaolin, GUI Ling, ZHU Qian, LU Feng
Accepted Manuscript  doi: 10.12206/j.issn.1006-0111.202110091
Abstract(1075) HTML(698) PDF (985KB)(25)
As an increasingly mature analytical technique, surface-enhanced Raman spectroscopy has the ability to identify, detect, and even quantitatively measure many single substances in nature. However, in the actual sample analysis, the tested samples were often a mixed system of various substances, and it was impossible to accurately characterize the components of the mixed system only by relying on SERS technology. Therefore, SERS combined with other techniques to accurately determine the measured substances has become an inevitable trend. Through the combination, the deficiency of SERS in detection and characterization was improved, and the purpose of efficient, sensitive and accurate determination of substances to be measured was achieved.
The eye irritation test and pharmacokinetic study of tacrolimus-loaded cationic nanoemulsion-based in-situ gel in rabbits
LIN Xin, ZHANG Jialiang, ZHOU Xin, SONG Hongtao
Accepted Manuscript  doi: 10.12206/j.issn.1006-0111.202104055
Abstract(846) HTML(532) PDF (1145KB)(8)
  Objective  To study the eye irritation and the pharmacokinetics of tacrolimus-loaded cationic nanoemulsion-based in-situ gel in rabbits.   Methods  The eye irritation of tacrolimus-loaded cationic nanoemulsion-based in-situ gel in rabbits was observed by histological cross-sections of external ocular tissues stained with HE. The aqueous humor of rabbit eyes was extracted by corneal puncture and analyzed by HPLC-MS for pharmacokinetic study.   Results  Tacrolimus-loaded cationic nanoemulsion-based in-situ gel had no significant irritation on rabbit eyes. The pharmacokinetic parameter showed that the AUC of Tacrolimus-loaded cationic nanoemulsion-based in-situ gel was (128.34±13.09) ng·h/ml, which was 1.13 times of tacrolimus-loaded cationic nanoemulsion (113.61±12.36) ng·h/ml and 1.88 times of Talymus® (68.25±10.82) ng·h /ml.   Conclusion  Tacrolimus-loaded cationic nanoemulsion-based in-situ gel had the advantages of low irritation, long retention time and high bioavailability in rabbit eyes. It has a good potential for clinical application.
Study on the rules of Traditional Chinese Medicine to promote wound healing based on data mining
LEI Zhongwei, DU Jie, SUN Hongyi, GU Qinwufeng, YANG Yanlong, LI Bai
Accepted Manuscript  doi: 10.12206/j.issn.1006-0111.202104099
Abstract(884) HTML(611) PDF (987KB)(14)
  Objective   To provide new ideas for promoting wound healing by digging and sorting out the medication rules in ancient classics and modern literatures.   Methods   The prescriptions for promoting wound healing recorded in literatures were collected to establish the database. The data mining technology was used for the analysis.   Results   75 prescriptions and 203 traditional Chinese medicines were recorded in the ancient TCM literatures for promoting wound healing. The core medicines included frankincense, liquorice, angelica sinensis, angelica dahuricae, cortex phellodendri, myrrh, etc. They mainly belong to the class of clearing-heat drugs, promoting-circulation drugs, reinforcing drugs, relieving drugs, detoxification and tissue granulation drugs. Cluster analysis and association rule analysis were conducted for 16 core drugs. 4 cluster combinations ,15 groups of drug pairs and drug group association rules were obtained.   Conclusion   The prescription rules for wound healing mainly included clearing heat, promoting circulation, reinforcing, relieving, detoxification, and promoting tissue granulation. TCM wound treatment should be based on syndrome differentiation for fever, blood stasis, deficiency, anabrosis, exterior syndrome and poisoning.
Mechanism of Psoralea corylifolia Linn on liver injury based on bioinformatics
YU Dan, CHI Xiaowei
Accepted Manuscript  doi: 10.12206/j.issn.1006-0111.202103054
Abstract(934) HTML(626) PDF (1187KB)(10)
  Objective  To explore the mechanism of Psoralea corylifolia Linn (PCL) on liver injury by establishing the biological function and pathway network of PCL components, targets and protein interactions based on bioinformatics.   Methods  The components of PCL and potential liver-injury related targets were collected from TCMIP database. The targets of PCL were predicted by the reverse pharmacophore matching method. Cytoscape software was applied for the construction of active components-targets network map. Protein-protein interaction network was constructed by STRING database. Gene ontology functional enrichment analysis and KEGG pathway enrichment analysis were conducted to predict the liver injury mechanism of PCL.   Results  22 components were identified from PCL with the corresponding 31 potential liver injury targets, mainly on serum albumin (ALB), glutathione S-transferase P (GSTP1), transthyretin (TTR) and peroxisome proliferator activated receptor gamma (PPARG) by PPI network analysis. The chemical carcinogenesis, adenosine 5 '- monophosphate activated protein kinase (AMPK) signal, PPAR signal, liver enzyme P450 and its harmful substance metabolism, glutathione metabolism and other signaling pathways were selected by KEGG analysis.   Conclusion  The active components of PCL may target on ALB, GSTP1, TTR and PPARG to regulate AMPK and PPAR signaling pathways, leading to liver injury.
Studies on orally disintegrating tablet of diphenhydramine hydrochloride and caffeine
FAN Li, ZENG Yuan, XIE Xiangyang, LIU Hui, XIONG Jing, ZHANG Ying, YUAN Yuan
Accepted Manuscript  doi: 10.12206/j.issn.1006-0111.202108090
Abstract(1046) HTML(538) PDF (869KB)(16)
  Objective  To optimize the formulation and preparation of diphenhydramine hydrochloride and caffeine orally disintegrating tablet.   Methods   Melt granulation technology of steric acid and API was used to mask the unpleasant tasting of diphenhydramine hydrochloride. The tablets were prepared by direct pressing the dry powder with CCMC-Na as disintegrating agent. The formulation was optimized by orthogonal experiments to achieve the shortest disintegration time and the best taste correction.   Results   The optimized formula of orally disintegrating tablet was as follows: diphenhydramine hydrochloride 25 mg, caffeine 60mg, stearic acid 25 mg, aspatan 40mg, blueberry essence 7mg, mannitol 45 mg, MCC 210mg, CCMC-NA 25 mg, SDS 8mg and magnesium stearate 5 mg.  Conclusion  This preparation method for orally disintegrating tablet of diphenhydramine hydrochloride and caffeine is practical and easy for quality control.
Hexokinase inhibitor 2-deoxyglucose combined with phenformin induces cell apoptosis of triple-negative breast cancer
YE Linlan, HE Chunhui, Zhu Xuting, LI Xia
Accepted Manuscript  doi: 10.12206/j.issn.1006-0111.202103045
Abstract(871) HTML(543) PDF (1601KB)(5)
  Objective   To investigate the effect of phenformin combined with hexokinase inhibitor 2-deoxyglucose (2-DG) on the treatment of triple-negative breast cancer cell lines 4T1 and MDA-MB-231.   Methods   Following treatment with phenformin, 2-DG or phenformin combined with 2-DG on 4T1 and MDA-MB-231 cells for 48 h, the cell proliferation in each group was detected by SRB and the apoptosis of cells was detected by flow cytometry. The concentration of glucose and lactic acid in cell culture supernatant was detected by ELISA. The activity of mitochondrial respiratory chain complex Ⅰ was detected by FlexStation3 and the mitochondrial oxygen consumption (OCR) was assayed with the Seahorse X Fe Analyzer.   Results   The hexokinase expression (4.6±0.17,3.73±0.21), glucose consumption (356±31, 397±42) μg/105 cells , Lactic acid concentration (5.59±0.52, 7.83±0.78) μmol/L in the supernatant of AT1 and MDA-MB-231 cells in Phenformin group were higher than that in control group ( 1±0.15, 1±0.12 ) , ( 289±25, 301±32) μg/105cells , ( 2.37±0.18, 4.01±0.45) μmol/L (P < 0.01). Even if the dose was reduced by 90%, the cell viability of phenformin combined with 2-DG group (64.63±2.28, 51.97±2.29) % was still higher than that of phenformin group (86.70±1.83, 85.53±1.46) % (P<0.001). The combination of the two drugs significantly promoted the apoptosis of AT1 and MDA-MB-231. In addition, compared with the phenformin group (5.59±0.52, 7.83±0.78) μmol/L, the phenformin combined with 2-DG group (3.46±0.37, 5.18±0.62) μmol/L cell lactic acid production also greatly reduced (P<0.01). Compared with the phenformin or 2-DG single-drug group, the phenformin combined with 2-DG group can significantly inhibit the growth rate of tumors in tumor-bearing mice (P<0.01). The median survival time of tumor-bearing mice in the phenformin combined with 2-DG group was 72.5 d, which was higher than that in the phenformin group 57 d and 2-DG group 55.5 d (P<0.01).   Conclusion   Hexokinase inhibitor 2-DG significantly enhances the therapeutic effects of phenformin on triple-negative breast cancer cells.
Research on quality control standard and safety of liquid bandage
XU Hang, WANG Tianyu, ZHANG Lingna, SONG Hongtao
Accepted Manuscript  doi: 10.12206/j.issn.1006-0111.202103063
Abstract(861) HTML(609) PDF (1055KB)(17)
[Abstract]Objective To investigate the quality and safety of liquid bandage and establish a reliable quality control method.   Methods  The quality of liquid bandage was evaluated by appearance, film forming time, viscosity, comfort, waterproof and air permeability. The content was determined by HPLC. The safety of liquid bandage was investigated with skin irritation test and allergy test.   Results  The self-made liquid bandage was uniform and delicate, with good ductility, fast film formation, good mechanical strength, waterproof and air permeability. The content of active pharmaceutical ingredients in the film met the requirements. It had no obvious irritation to the skin and no allergic reaction.   Conclusion  The established quality control method was reliable. The liquid bandage had good safety profile.
Research overview of anti-osteoporosis effects of traditional animal medicines
ZHANG Jingwen, XIA Tianshuang, JIANG Yiping, XIN Hailiang
Accepted Manuscript  doi: 10.12206/j.issn.1006-0111.202101032
Abstract(1008) HTML(666) PDF (961KB)(17)
[Abstract] Osteoporosis is a systemic bone metabolism disease characterized by low bone mass, bone microstructure destruction, increased bone fragility, and easy fracture, which is more common in the elderly. Animal medicine, as an important part of natural medicines, has the characteristics of wide resources, complex chemical components, and broad pharmacological effects. It has been extensively used in the field of anti-osteoporosis. This article summarizes the pharmacological effects and applications of several major animal medicines for osteoporosis, and discusses the existing problems, aiming to provide a reference for the development of animal drugs against osteoporosis.
Diphenhydramine hydrochloride asaay in compound diphenhydramine cream by HPLC
LU Songwei, LUO Jinqian, YU Huihua, CHEN Fangjian
Accepted Manuscript  doi: 10.12206/j.issn.1006-0111.202102016
Abstract(960) HTML(549) PDF (893KB)(13)
  Objective  To establish the HPLC method of assay for diphenhydramine hydrochloride in compound diphenhydramine cream.   Method   HPLC analysis was carried on ACE5C18S/N-A66766 column (150 mm×4.6 mm, 5 µm) with the mobile phase of methanol-water-triethylamine (70:30:0.67, adjusting pH to 6.50 with phosphoric acid) at room temperature. The detection wavelength was set at 230 nm and the flow rate was 1.0 ml/min. The analyte was extracted from the cream and 20 μl of sample was injected.   Results  The calibration curve of diphenhydramine hydrochloride was linear in the range of 39.52-197.6 µg/ml (r=0.999 7). The average recovery of diphenhydramine hydrochloride was 100.5% (RSD=1.25%, n=9). The repeatability of the method was expressed using RSD with 0.78% (n=6). The results of assay were 101.3%、99.83%、99.62%.   Conclusion   The method is accurate, sensitive, selective and repeatable, which can be applied for improving the quality standard of compound diphenhydramine cream.
The Standard Specification of Microscopic Identification of Chinese Pharmacopoeia 2020 Edition
LU Lun, ZHANG Chengzhong
Accepted Manuscript  doi: 10.12206/j.issn.1006-0111.202103079
Abstract(995) HTML(981) PDF (878KB)(13)
[Abstract]Objective To improve the standardization and legitimacy of the quality control for traditional Chinese medicine (TCM) by analyzing the microscopic identification quality standard of TCM in Chinese Pharmacopoeia 2020 edition (Volume 1).   Methods   Through the analysis of the standard items of microscopic identification in Chinese Pharmacopoeia, 2020 edition (Volume1), the problems in the standard was summarized and classified , and suggestions for revision were provided.   Results   The standardization and consistency have the room to improve in TCM microscopic identification standard in Chinese Pharmacopoeia 2020 (Volume 1).   Conclusion   The TCM microscopic identification standard needs to be improved, and the formulation for the standard should be more specific and practical.
In Press
Abstract(1614) HTML(986) PDF (663KB)(187)
The pharmaceutical care by clinical pharmacists for a foot injury patient with postoperative MRSA infection
LUO Jingjing, WU Xinan, Xue Gang, Xie Zhengdong, Ye Jianbo, RONG Chengting
In Press  doi: 10.12206/j.issn.1006-0111.202102004
Abstract(2853) HTML(1197) PDF (871KB)(52)
  Objective  To explore the clinical pharmacist’s role in drug therapy and monitoring for the patient with postoperative MRSA infection.   Methods  Clinical pharmacists participated in planning anti-infective treatment, antimicrobial medication selection, identification of high-risk factors for MRSA infection, evaluation of vancomycin efficacy, dosage adjustment using TDM technology, monitoring and management of vancomycin adverse reactions.  Results  With the pharmaceutical care provided by clinical pharmacists, the patient received effective treatment with minimal vancomycin-related adverse reactions.   Conclusion  Clinical pharmacists played an important role in accelerating patient recovery by rational and safe medication use.
Advances in mosquito repellents
CHEN Xinlu, TAI Zongguang, ZHU Quangang, GAO Shen
Accepted Manuscript  doi: 10.12206/j.issn.1006-0111.202103036
Abstract(2576) HTML(835) PDF (898KB)(59)
Mosquitoes are the biological vectors of many diseases. Mosquito-borne infectious diseases are serious public health problems in tropical areas. With the increasing resistance of mosquitoes to insecticides, it becomes difficult to control mosquito-borne infectious diseases. The application of mosquito repellents can not only control the spread of mosquito-borne infectious diseases to a certain extent, but also reduce the use of insecticides and relieve the environmental pressure. This paper introduces and summarizes the research progresses of new mosquito repellents in recent years to provide reference resource for the further development of mosquito repellents.
Advances in prevention and treatment for motion sickness
ZHANG JingXiang, ZHU Lin, XING XinHao, WANG XinRong, WANG Yan
Accepted Manuscript  doi: 10.12206/j.issn.1006-0111.202101018
Abstract(1838) HTML(919) PDF (854KB)(228)
Motion sickness is a series of physiological responses in human being caused by abnormal movement stimulation. With the development of science and technology, a growing number of people choose to travel by high speed vehicles. Motion sickness happens more frequently. A large number of non-drug and drug intervention methods have been reported in the treatment of motion sickness. This article provides an overview on the research developments in the prevention and treatment of motion sickness in order to provide new ideas for drug research.
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The latest research progress of α7 nicotinic acetylcholine receptor in cholinergic anti-inflammatory pathway
ZHANG Huimin, XIANG Kefa, SHI Xiaofei, QIN Zhen, LIU Xia
2022, 40(6): 485-493.   doi: 10.12206/j.issn.2097-2024.202204037
Abstract(471) HTML(263) PDF (1308KB)(14)
α7 nicotinic acetylcholine receptor (α7nAChR) belongs to nicotinic acetylcholine receptors (nAChRs), which is the key receptor in cholinergic anti-inflammatory pathway and plays an important role in the neural regulation of immune system. Recent studies have found that α7nAChR is also involved in the regulation of various physiological and pathological processes other than immunity, such as non-alcoholic fatty liver, angiogenesis, heart protection, etc. In addition, α7nAChR is closely related to energy metabolism in neurodegenerative diseases. This article reviews the role of α7nAChR in treating inflammation and improving energy metabolism, and the new direction of α7nAChR involved in therapy.
Study on polymorphism of peritoneal microbial community after laparoscopic exploration in New Zealand rabbits based on 16S rDNA sequencing
ZHANG Pingping, WU Wenbin, CAO Qi, QU Zhuo, WANG Pei
2022, 40(6): 494-498, 504.   doi: 10.12206/j.issn.2097-2024.202201016
Abstract(599) HTML(419) PDF (1891KB)(9)
  Objective  To explore the polymorphism of microbial community after laparoscopic abdominal exploration by using bacterial 16s ribosomal DNA (16S rDNA) sequencing technology.   Methods  New Zealand rabbits were divided into model group and control group. The rabbits in the model group were operated by portable laparoscopy, and the rabbits in the control group were not treated. One week later, the peritoneal effusions of the model group and the control group were taken for 16S rDNA sequencing to analyze the microbial community polymorphism. To explore the changes of microbial community in peritoneal effusion in the model group compared with the control group.   Results  After 16S rDNA sequencing, bioinformatics was used to determine the microbial communities. Inter group difference analysis showed a good similarity of microbial communities between the two groups. OTU taxonomic analysis and species composition analysis (Rank-Abundance curve and Venn diagram) found that the microbial community level of the model group was significantly higher than that of the control group. Alpha diversity analysis (Sobs, Ace, Shannon, Simpson) showed that the richness and diversity of microbial community in the model group were higher than those in the control group. Microbial composition analysis showed that the number of miscellaneous bacteria in the model group increased by about 30% compared with the control group. The species differences between the two groups were tested for significance. It was found that Pasteurellales, Neisseria and Tsukamurella increased significantly.   Conclusion  The diversity of microbial communities in peritoneal effusion increases after laparoscopic abdominal exploration in New Zealand rabbits, and the most significant increases are Pasteurella, Neisseria and Tsukamura.
Study on the secondary metabolites from a polar marine sponge associated actinomyces Streptomyces sp. LHW11-07
LU Jingrong, LIU Yawei, DAI Ying, LIN Houwen, YANG Fan
2022, 40(6): 499-504.   doi: 10.12206/j.issn.2097-2024.202206081
Abstract(389) HTML(166) PDF (1003KB)(7)
  Objective  In order to obtain small molecule compounds with novel structure and good biological activity, the secondary metabolites of polar sponge-symbiotic Streptomyces sp. LHW11-07 were studied.   Methods  The fermentation product of Streptomyces sp. LHW11-07 was isolated and purified by gel column chromatography, silica gel column chromatography, reversed-phase medium pressure column chromatography and high performance liquid chromatography. The structures of the monomeric compound were identified by modern spectroscopic methods such as mass spectrometry, nuclear magnetic resonance and related literature reports.   Results  A total of nine compounds were isolated from the fermentation of this strain, which were cyclo-(L-Tyr-L-Trp) ( 1 ), cyclo-(L-Trp-L-Ser) ( 2 ), cyclo-(D-Tyr-D-Pro) ( 3 ), cyclo-(L-Tyr-L-Phe) ( 4 ), cyclo-(L-Tyr-L-Leu) ( 5 ), albaflavenol B ( 6 ), β-adenosine ( 7 ), N-formylantimyic acid methyl ester ( 8 ) and conglobatin A ( 9 ).   Conclusion  Compounds 1 and 2 were isolated from Streptomyces sp. for the first time.
Wilforine inhibits LPS-induced inflammatory response in RAW264.7 cells by regulating the TLR4/MyD88/TRAF6 signaling pathway
WANG Ying, ZHANG Minxin, LIN Bing
2022, 40(6): 505-509.   doi: 10.12206/j.issn.2097-2024.202111068
Abstract(528) HTML(222) PDF (1682KB)(15)
  Objective  To investigate the anti-inflammatory effect and mechanism of wilforine.   Methods  Anti-inflammatory activity of wilforine was investigated in LPS-induced RAW264.7 cells. The cytokines production of RAW264.7 cells was analyzed by ELISA assay and the cell viability was assessed by CCK-8 method. The expression of TRAF6, the phosphorylation of IRAK, p38, ERK and JNK, the degradation of inhibitory κBα (IκBα) and the nuclear translocation of NF-κB p65 were further investigated by western blot.   Results  Triptolide had no significant toxicity to RAW264.7 cells at concentrations of 25, 50 and 100 μmol/L. and could significantly inhibit the contents of cytokines NO, IL-1β, TNF-α and IL-6. Wilforine significantly decreased the expression of TRAF6 and phosphorylation of IRAK, and inhibited the phosphorylation of ERK, p38, and JNK and degradation of IκBα, and reduced the level of nuclear translocation of NF-κB p65.   Conclusion  The anti-inflammatory activity of wilforine of LPS-induced RAW264.7 cells is probably via TLR4/MyD88/TRAF6 signaling pathway.
Study on hypoglycemic effect and intestinal effect of Polygonatum sibiricum polysaccharides in diabetic mice
REN Qunli, ZHANG Xinqun, WANG Miao, LI Xiaolan, YAO Yanzi, RAN Yinghui, WANG Qian
2022, 40(6): 510-514.   doi: 10.12206/j.issn.2097-2024.202206057
Abstract(385) HTML(159) PDF (2046KB)(6)
  Objective  To study the hypoglycemic effect of Polygonatum sibiricum polysaccharides on type 2 diabetic mice and its effects on intestinal flora and pathological structure of small intestine.   Methods  Fifty male mice were used, except 10 were fed normally, the others were fed with high-fat and high-sugar diet for 6 weeks, and then injected with streptozotocin intraperitoneally to make type 2 diabetes mice model. After successful modeling, they were randomly divided into model group and Polygonatum sibiricum polysaccharides (500, 250, 125 mg/kg) group and the model group mice were given normal saline. The changes of bodyweight and blood glucose of mice in each group were recorded. After 4 weeks, feces were collected and sequenced by a 16S rRNA high-throughput sequencing, and the pathological changes of small intestine were observed by HE staining.   Results  In diabetic mice, the weight decreased. After given Polygonatum sibiricum polysaccharides, the weight of mice increased by 14.24%,11.97% and 8.78%, and the blood glucose decreased by 26.6%, 22.3% and 13.3%, respectively after high, medium and low doses of Polygonatum sibiricum polysaccharides were administered. In addition, the pathological disorder and swelling of intestinal histopathology were improved. There were significant differences in intestinal microorganisms between the model group and the Polygonatum sibiricum polysaccharides. Verrucomicrobiae in the model group increased significantly, while the microorganism abundance of Firmicutes and Bacteroidetes in the healthy group and Polygonatum sibiricum polysaccharides group was higher.   Conclusion  Polygonatum sibiricum polysaccharides has a significant hypoglycemic effect on diabetic mice and a certain protective effect on their intestines, the mechanism may be achieved by increasing the richness of beneficial bacteria and improving the immune function of mice, it’s in a certain dose-effect relationship, and its immune function needs further study.
Study on intestinal absorption characteristics of apixaban by in vivo one-way perfusion in rats with renal failure
ZHANG Huan, WANG Li, ZHANG You, XIE Pei, LI Na, DING Jiarong, WANG Lei
2022, 40(6): 515-518, 531.   doi: 10.12206/j.issn.2097-2024.202112012
Abstract(720) HTML(371) PDF (991KB)(14)
  Objective  To study the intestinal absorption characteristics of apixaban in rats with renal failure, and the effect of P-glycoprotein (P-gp) inhibitors on its absorption behavior.   Methods  The in vivo absorption experiment was performed in CRF rats by one-way perfusion method and the absorption factors was investigated by establishing the HPLC analysis method.   Results  The absorption rate constant (Ka) of apixaban in each intestinal segment was significantly different (P<0.05) with no significant difference in apparent absorption coefficient (Papp) (P>0.05). The Ka and Papp values in the rat ileum decreased with the increasing of drug concentration. After addition of P-gp inhibitor verapamil hydrochloride (0.1 mmol/L), the Ka and Papp values of apixaban in the jejunum and ileum were significantly increased.   Conclusion  Apixaban is absorbed in all intestinal segments. P-gp inhibitors can significantly promote the absorption of apixaban in jejunum and ileum, suggesting that apixaban is P-gp substrate and its absorption mechanism is supposed to be active transport.
Mechanism of Kunxian capsule in the treatment of rheumatoid arthritis based on network pharmacology
YUAN Zike, LIU Minglin, DU Juan, YAO Rubing
2022, 40(6): 519-525, 545.   doi: 10.12206/j.issn.2097-2024.202104112
Abstract(1072) HTML(619) PDF (1584KB)(41)
  Objective  To explore the molecular targets and associated potential pathways of Kunxian capsule in the treatment of rheumatoid arthritis (RA) based on network pharmacology.  Methods  The constituents of Kunxian capsule were searched by Traditional Chinese Medicine Systems Pharmacology Database, Analysis Platform (TCMSP) and a high-throughput experiment- and reference-guided database of traditional Chinese medicine(HERB).The potential active ingredients and targets were retrieved based on TCMSP database. RA related gene targets were retrieved through GeneCards database and OMIM database. Venn online software was used to obtain the common target of drugs and diseases. The “compound-target” network diagram was constructed with Cytoscape software. String database was used to draw the protein interaction (PPI) network. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of the intersection network were conducted by Bioconductor Database.   Results  81 active ingredients and 913 targets were identified. 228 targets were obtained after removing the duplicates. 4494 target genes directly related to RA were obtained from the GeneCards databases and OMIM databases. 162 genes were obtained from the intersection of component-target and disease-target. It was revealed that five ingredients including quercetin, luteolin, kaempferol, β-sitosterol and triptolide are the main active ingredients in Kunxian capsule. AKT1, IL-10, STAT3, CASP8 and CDKN1A may be the main therapeutical targets. The results of GO and KEGG enrichment analysis showed that the mechanism of Kunxian capsule is mainly related to the regulation of reactive oxygen metabolism, the regulation of kinase activity, and IL-17 signaling pathway. The important biological processes and signaling pathways include infection, inflammation and immunity.   Conclusion  This research preliminarily explored the mechanism of Kunxian capsule in the treatment of RA by network pharmacology and suggested that the overall regulation is characterized by multi-components, multi-targets, and multi-channels. It provided some ideas for further molecular biology experiments.
The improvement of formulation process and quality evaluation of coenzyme Q10 emulsion
WANG Yu, DENG Yihui, SONG Yanzhi, LIU Guangxuan
2022, 40(6): 526-531.   doi: 10.12206/j.issn.2097-2024.202106117
Abstract(757) HTML(289) PDF (1465KB)(14)
  Objective   To evaluate the quality of coenzyme Q10 emulsion with improved formulation and technology and establish an assay method.   Methods   Coenzyme Q10 emulsion with a high oil concentration was prepared and analyzed by HPLC. The physical and chemical properties of the emulsion were characterized, and the entrapment efficiency was determined. The stability of sterilization, freeze-thaw and dilution was investigated. The photodegradation test as well as the influencing factors, acceleration and long-term stability tests were carried out.   Results   The particle size, Zeta potential, pH value, content and entrapment efficiency of coenzyme Q10 emulsion were (239.5±0.8) nm、(−32.28±2.04) mV、(5.86±0.02)、 (100.59±1.24) % and (98.5±1.1) %, respectively. The stability of sterilization, freeze-thaw and dilution was good. The photolysis rate was directly proportional to the dilution ratio and inversely proportional to the drug loading. Coenzyme Q10 emulsion should be prepared in light free environment and stored at a low temperature. The pH value dropped 0.61 when it was kept in darkness at (40±2) ℃ for 10 days. It exhibited good stability both in the accelerated and long-term test.   Conclusion   The physicochemical properties of coenzyme Q10 emulsion with a high oil concentration meet the quality requirements for intravenous injection with good stability.
Freeze/thaw stability and quality control chart analysis of metronidazole sodium chloride injection
ZHU Dianqian, LI Ruimin, LI Ling
2022, 40(6): 532-535.   doi: 10.12206/j.issn.2097-2024.202105136
Abstract(641) HTML(305) PDF (1097KB)(8)
  Objective  To investigate the effect of different freezing thawing conditions on the stability of metronidazole sodium chloride injection in extreme cold environment.   Methods  The freezing process of metronidazole sodium chloride injection in extremely cold environment was simulated and appropriate conditions were given to melt. The effects of room temperature, 60 ℃ water bath thawing and freeze-thaw times on the content of metronidazole were investigated by HPLC, and the changes were analyzed by quality control chart.   Results  The linear range of metronidazole was 0.01~1.00 mg/ml and the relationship was acceptable, r=1.000, the average recovery was 101.03% (RSD=1.17%), which met the requirements of methodology. The samples were frozen at −20 ℃ and thawed in 60 ℃ water bath for 18 times with good stability. However, when thawed at room temperature, acicular crystals formed and the content decreased.   Conclusion  In extreme cold environment, the stability of metronidazole sodium chloride injection could be affected by freezing and thawing conditions. Therefore, in the use of Metronidazole and Sodium Chloride Injection, the number of freeze-thaw cycles should be minimized, and try to avoid thaw at room temperature.
The acute toxicity of tetrodotoxin to zebra fish
WANG Haoneng, ZHANGJI Qianzhu, LI Jingfeng, JIA Rui, ZHU Yuping, ZHU Jiangbo, CHEN Jikuai, YAN Lang
2022, 40(6): 536-539.   doi: 10.12206/j.issn.2097-2024.202202083
Abstract(773) HTML(411) PDF (1513KB)(15)
  Objective  To study the acute toxicity of tetrodotoxin to zebra fish.   Methods  The maximum non-lethal concentration (MNLC) and 10% lethal concentration (LC10) determinations were used to assess the acute toxicity of tetrodotoxin.   Results  According to the simulation calculation of Origin 8.0 software, the MNLC was 8.62 µmol/L and 15.2 µmol/L for LC10. Under the experimental conditions, tetrodotoxin at a concentration of 16.0 µmol/L and above induced pericardial edema and arrhythmia, leading to the death of zebra fish. The target organs for acute toxicity of tetrodotoxin were the heart and liver. The main manifestations were pericardial edema, arrhythmia, and delayed yolk sac absorption. The toxicity appeared at a concentration of 0.958 µmol/L.   Conclusion  Tetrodotoxin has heart and liver toxicity to zebra fish, and its toxicity is dose-dependent.
Analysis of volatile organic compounds in urine of diabetic patients comorbid with depression
GUO Zhijun, DING Xuyin, LI Zhuman, MA Yunpeng, WANG Yan, YU Xuemei, ZHANG Min, XU Feng
2022, 40(6): 540-545.   doi: 10.12206/j.issn.2097-2024.202206045
Abstract(412) HTML(152) PDF (1977KB)(6)
  Objective  To compare the difference of volatile organic compounds (VOCs) in the urine of diabetic patients comorbid with or without depression.   Methods  Patients with type 2 diabetes who met the diagnostic criteria of prevention and treatment guidelines and were treated in inpatient department of Sixth People’s Hospital South Campus, Shanghai Jiaotong University, from November 2019 to November 2020 were included in the study. Depression screening was performed using PHQ9 scale. According to the scores, the patients were divided into two groups: diabetic patient group and diabetic patient comorbidity depression group. The morning urine was collected and VOCs in urine were detected using a miniature gas tester.   Results  A total of 161 patients with diabetes mellitus, including 141 patients in the diabetic group and 20 patients in the diabetic comorbidity depression group. The results indicated that the sensitivity, specificity and accuracy of the method are 80%, 98% and 92.8% respectively. There were differences in urine volatile organic compounds between the two groups.   Conclusions  Compared with diabetic patients, the composition of volatile organic compounds in urine may change after diabetic comorbidity depression, which can provide experimental reference for early clinical diagnosis of depression.
Study on quality standard improvement for Gansu granules
TAO Chaoyang, SHI Pengjie, ZHOU Xin, SUN Lianna
2022, 40(6): 546-549.   doi: 10.12206/j.issn.2097-2024.202207061
Abstract(426) HTML(139) PDF (1656KB)(8)
  Objective  To establish the quality standard of Gansu granules (GGs) and effectively control the quality of GGs.   Methods  TLC identification method of Gansu granules was improved by using pinocembrin-7-O-β-D-glucoside as the indicator. In HPLC analysis, Agilent ZorbaxSB-C18 (4.6 mm×250 mm, 5 μ m) was used as a chromatographic column, and acetonitrile-0.5% formic acid solution was used as the mobile phase. The detection wavelength of 280 nm was used to analyze the content of pinocembrin-7-O-β-D-glucoside in GGs.   Results  The separation of characteristic spots was good and clear when the established TLC method was used to identify Gansu granules. Quantitative analysis showed that the concentration of pinocembrin-7-O-β-D-glucoside has a good linear relationship with the peak area in the range of 2.4-240 µg/ml (r= 0.9999). The average sample recovery rate of pinocembrin-7-O-β-D-glucoside is 100.76% with RSD value of 0.66%. The accuracy was good.   Conclusion  The TLC identification method and HPLC content determination method established in this experiment have strong specificity and good reproducibility, and can be used as an improved standard for the quality control of Gansu granules.
Acetaminophen content assay with UHPLC in the sustained-release tablet exposed to radiation
YU Dexun, HUANG Xinhui, ZHU Kehan, HUANG Ting, ZHOU Tingting, GAO Jianyi
2022, 40(6): 550-552, 562.   doi: 10.12206/j.issn.2097-2024.202109100
Abstract(2050) HTML(597) PDF (957KB)(14)
  Objective  To assay the contents of acetaminophen with ultra high-performance liquid chromatography (UHPLC) method in the sustained-release tablets radiated by Gamma ray.   Methods   Acetaminophen sustained-release tablets were radiated by 60Co. UHPLC equipped with the Shim-pack GISS-C18(2.1 mm×50 mm, 1.9 μm)was used for the assay. The mobile phase was methanol-0.05 % ammonium acetate solution (15∶85). The flow rate was 0.3 ml/min with the detection wavelength at 245 nm.   Results  The acetaminophen showed good linear relationship within the range of 20-100 μg/ml (r=0.999 4). The RSD values of repeatability was 0.9 %. The average recovery was 97.9 %-104.9 %. Acetaminophen content was 96.2 %, 92.2 %, 91.8 %, 83.9 % at 0, 8, 50 and 80 kGy radiation, respectively.   Conclusion  This method is speedy and accurate. It can be used to assay the content of acetaminophen in the sustained-release tablets after radiation. The content of acetaminophen decreased after radiation.
Simultaneous determination of seven constituents in Chenxiang Lubailu tablet by HPLC
ZHANG Lu, DAI Qunfang, CHEN Sisi
2022, 40(6): 553-556, 570.   doi: 10.12206/j.issn.2097-2024.202202038
Abstract(705) HTML(432) PDF (1036KB)(14)
  Objective  To establish the method for the simultaneous determination of liquiritin, ammonium glycyrrhizinate, hesperidin, nobiletin;tangeretin;costunolide, dehydrocostuslactone in Chenxiang Lubailu tablet by HPLC.   Methods   ZORBAX Eclipse XDB-C18 chromatographic column (4.6 mm×250 mm, 5 μm) was used. The mobile phase was methanol-0.1% phosphoric acid solution. Gradient elution with flow rate of 1.0 ml/min was used. Column temperature was 35 ℃. Detection wavelength for liquiritin, ammonium, tangeretin, and costunolide was at 237 nm. Detection wavelength for glycyrrhizinate was at 283 nm. Detection wavelength for hesperidin and nobiletin was at 330 nm. Injection volume was 10 μl. 16 batches of samples were tested.   Results  The linear ranges for the detection of liquiritin, ammonium, glycyrrhizinate, hesperidin, nobiletin, tangeretin, and costunolide were 1.110 - 55.72 (r=0.9992), 22.15 - 1108 (r=0.9999), 6.140 - 307.2 (r=0.9995), 1.130 - 56.25 (r=0.9997), 0.3700 - 18.75 (r=0.9982), 0.5200 - 26.01 (r=0.9991), and 1.180 - 58.95 (r=0.9999) μg/ml respectively. The average recoveries were 98.71%, 98.12%, 98.44%, 98.22%, 99.17%, 99.18%, and 97.93%, and the RSDs were 0.16%, 0.67%, 0.57%, 0.62%, 0.48%, 0.56%, and 0.58% respectively. The contents of the seven components in 16 batches of samples were 0.1250 - 1.174, 2.354 - 7.426, 1.822 - 27.21, 0.0370 - 1.399, 0.0723 - 0.4433, 0.0140 - 0.1990, and 0.2207 - 1.407 mg/g respectively.   Conclusion  The method is accurate, reproducible and durable, which could be used to the quality control and evaluation of Chenxiang Lubailu tablet.
Study on quality standards of volatile oil of Chunyang Zhengqi capsules
WEI Feixue, LI Xiaoju, XIONG Sisi, LIAO Yunfang, CHEN Min
2022, 40(6): 557-562.   doi: 10.12206/j.issn.2097-2024.202201042
Abstract(506) HTML(286) PDF (2390KB)(11)
  Objective   To establish a quality control system of volatile oil of Chunyang Zhengqi capsules.   Methods  The chromatogram of volatile oil was established by GC method, and the contents of cinnamaldehyde and eugenol were determined.   Results  In 15 batches of samples, 19 common peaks were identified, and 9 characteristic peaks were selected to establish the characteristic spectrum. The linear ranges of cinnamaldehyde and eugenol were 0.522 - 1.565 mg/ml (r=0.9994) and 3.038 - 9.115 mg/ml (r=0.9997), respectively. The average recoveries were 97.1% and 97.3%, with RSD of 1.5% and 1.4%, respectively.   Conclusion  The established GC characteristic map and content determination method could control the quality of essential oil in Chunyang Zhengqi capsules qualitatively and quantitatively. The method is accurate and feasible which could be used as the quality control method of essential oil in Chunyang Zhengqi capsules.
Study on the mechanism of anti-inflammatory and hepatoprotective effects of Shuganning injection based on network pharmacology
XIN Bao, XIE Fangyuan, YAO Chen, GE Jiyun, LIU Jing, ZHANG Jing, MIAO Hong, HUANG Tingting, HUANG Yufeng
2022, 40(6): 563-570.   doi: 10.12206/j.issn.2097-2024.202204049
Abstract(682) HTML(520) PDF (1929KB)(20)
  Objective  To explore the anti-inflammatory and hepatoprotective mechanism of Shuganning injection through establishing the active ingredients-targets network and protein interactions network.   Methods  The main active ingredients of Artemisiae scopariae, Fructus gardenia, Radix scutellariae, Radix isatidis and Ganoderma in Shuganning injection were obtained by TCMSP; GeneCards and OMIM were used to screen the hepatitis-related targets among the corresponding targets of the active ingredient of Shuganning injection; The Cytoscape software was used to construct the active ingredient-targets network of Shuganning injection. The protein interactions network was constructed using the String database and Cytoscape software. The GO and KEGG pathways involved in the targets were analyzed by DAVID database.   Results  The results showed that 20 active ingredients and 83 targets of Shuganning injection were involved. GO analysis showed that Shuganning injection mainly affected the regulation of cellular processes and biological processes, as well as the response to chemical stimulation and stress. KEGG pathway analysis showed that the targets of the anti-inflammatory and hepatoprotective effect of Shuganning injection mainly involved in signaling pathways such as TNF, IL-17, and MAPK.   Conclusion  The anti-inflammatory and hepatoprotective effect of Shuganning injection have the characteristics of multiple components, multiple targets and multiple pathways, which may play a role by regulating pathways such as TNF、IL-17 and MAPK .
Effect of renal failure on docetaxel exposure and adverse reactions in breast cancer patients
ZHOU Juxiang, WANG Shuangying, SHE Jiantao, ZENG Xianliang, ZHANG Jihong
2022, 40(6): 571-575.   doi: 10.12206/j.issn.2097-2024.202203068
Abstract(338) HTML(171) PDF (870KB)(4)
  Objective  To investigate the influence of renal failure on the area under curve (AUC) and adverse reactions of docetaxel in breast cancer patients, and provide evidence for the dosage of docetaxel in renal failure patients.   Methods  A retrospective study was conducted on 24 patients with breast cancer who had undergone radical mastectomy and received AC-T adjuvant chemotherapy in our hospital from January 2019 to November 2021. According to renal function cases, the patients were divided into two groups: renal failure group (n=5) and normal renal function group (n=19). The clinical characteristics such as gender, age, body weight and body surface area of patients in two groups, docetaxel dose, blood concentration, area under the curve, liver and kidney function, white blood cell count and absolute value of neutrophil before chemotherapy were collected. Single factor linear regression was used to analyze the influencing factors of the AUC of docetaxel. Adverse reactions after chemotherapy with docetaxel including nausea and vomiting, bone marrow suppression, constipation and liver function injury were collected. CTCAE 4.0 evaluation standard was used to evaluate adverse reactions.   Results  The clinical characteristics of creatinine [908.0 (819.0, 1018.0) μmol/L vs 54.8 (52.0, 65.0) μmol/L] and creatinine clearance rate [4.9 (4.3, 5.4) ml /min vs 86.3 (59.3, 92.5) ml/min] of the renal failure group and the normal renal function group have significant difference (P<0.001), while no significant difference (P>0.05) were found in the body surface area [1.4 (1.4, 1.5) m2 vs 1. 6 (1.5, 1.6) m2], docetaxel dose [70.4 (69.4, 73.0) mg/m2 vs 74.4 (72.3, 91.2) mg/m2], body weight [(51.4±3.8) kg vs (51.5±5.5) kg]. Liver function, white blood cells and neutrophils were within the normal range before chemotherapy with docetaxel. There was no significant difference in AUC value [(1.6±0.6) mg·h/L vs (1.8±0.8) mg·h/L] between the two groups after chemotherapy with docetaxel (P>0.05). Linear univariate regression analysis indicated that the blood concentration at the end of docetaxel infusion was significantly associated with AUC of docetaxel (P<0.001), while the body surface area, dose of docetaxel, body weight, liver and kidney function were not correlated with AUC of docetaxel (P>0.05). After chemotherapy with docetaxel, adverse reactions of patients in the two groups: nausea and vomiting (grade I incidence: 40% vs. 57.9%, grade II incidence: 60% vs. 42.1%), myelosuppression (grade I incidence: 60% vs. 84.2%, grade II incidence: 20% vs 15.8%) and constipation (all mild constipation) had no significant difference (P>0.05).   Conclusion  Renal failure did not affect the exposure of docetaxel and the adverse reactions after chemotherapy with docetaxel in breast cancer patients.
Effects of Ulinastatin on inflammatory response, hemodynamics, PaO2/FiO2 and prognosis in patients with septic shock
WANG Xiaodan, MA Xiaolong, DONG Wenxing
2022, 40(6): 576-579.   doi: 10.12206/j.issn.2097-2024.202206091
Abstract(405) HTML(193) PDF (868KB)(14)
  Objective  To evaluate the effect of Ulinastatin injection on inflammation, hemodynamics, oxygenation index (PaO2/FiO2) and prognosis in patients with septic shock.   Methods  A retrospective analysis was performed on clinical data of 109 patients with septic shock from January 2017 to December 2019. Patients were divided into observation group (n=73) and routine group (n=54) according to the treatment regimens. The two groups were given anti-shock treatment according to the relevant guidelines, and observation group was additionally given Ulinastatin. The inflammatory factors, hemodynamic parameters and PaO2/FiO2 and recovery were compared between the two groups before and after treatment.   Results  The levels of IL-6, TNF-α and PCT at 7 d of treatment were significantly decreased in the two groups (P<0.05), and the levels of IL-6, TNF-α and PCT of observation group were lower than that of routine group (P<0.05). At 12 h, 24 h and 72 h of treatment, the MAP, CI and PaO2/FiO2 were significantly increased in the two groups (P<0.05) while the EVLWI and SVRI were significantly decreased (P<0.05), and the MAP and PaO2/FiO2 of observation group at the same time were higher than that of routine group while the EVLWI was lower than that routine group (P<0.05). At 7 d of treatment, the APACHE-II score, mechanical ventilation time and ICU stay time of observation group were lower than that of routine group, and the incidence rates of MODS in the two groups were 4.11% and 14.81% respectively (P<0.05), and the mortality rates were 1.37% and 7.41% respectively (P>0.05).   Conclusion  Ulinastatin could be beneficial in septic shock, which could reduce inflammatory response, improve hemodynamic parameters and microcirculation perfusion, and put a positive effect on promoting rehabilitation and improving prognosis.
Pharmaceutical care for a gastric cancer patient with post-operation abdominal infection and cellulitis
ZHANG Yunting, FU Hui, LI Dongjie, HUANG Fang, SHEN Fuming
2022, 40(6): 580-582.   doi: 10.12206/j.issn.2097-2024.202107101
Abstract(305) HTML(151) PDF (1443KB)(23)
  Objective   To explore the effective management of post-operation infections through clinical pharmacists participating in the treatment of a gastric cancer patient with post operation abdominal infection and cellulitis.   Methods  Clinical pharmacists provided an rational anti-infective treatment plan with the combination of pharmacy and clinical practice, conducted medication education for the patient, and participated in the process of pharmacy care for the patient.   Results   Physicians modified the medication plan according to the recommendations from the clinical pharmacists. The patient’s infection was effectively controlled and the symptoms were alleviated.   Conclusion   With their professional knowledge, clinical pharmacists can select the best antibacterial drug regimen for the treatment of post-operation infections and provide patients with optimal pharmaceutical care.
Analysis of the use for antiviral drugs in the treatment of COVID-19 patients in a Hubei hospital in 2020
PANG Suqiu
2022, 40(6): 583-586.   doi: 10.12206/j.issn.2097-2024.202009044
Abstract(774) HTML(450) PDF (858KB)(31)
  Objective  To retrospectively analyze the use of antiviral drugs in 1765 patients admitted to a hospital of COVID-19 in Hubei Province, and to provide experience and suggestions for clinical rational drug use.   Methods  The antiviral drugs used in a hospital in Hubei Province during the treatment of COVID-19 patients from February to April 2020 were analyzed according to the "New Coronary Virus Pneumonia Diagnosis and Treatment Program" recommendated by the National Health Commission.   Results  Among the 1765 patients, 1114 (63.11%) were treated with antiviral drugs. The top three antiviral drugs were arbidol (981 cases, use rate 55.58%), IFN-α-2b (340 cases, use rate 19.26%) and chloroquine (72 cases, use rate 4.08%) . Among all the antiviral regimens, 636 (57.09%) were treated with arbidol alone, followed by arbidol combined IFN-α-2b 164 (14.72%) , arbidol combined IFN-α-2b 98 (8.8%) , and the top three regimens accounted for 80.61% of all antiviral regimens. According to the COVID-19, IFN-α-2b, Arbidol, and chloroquine had more than 20% of the over-treatment duration, 6 patients used more than 3 antiviral drugs, and 50 patients had not been recommended for hydroxychloroquine.   Conclusion  The antiviral drugs used for the treatment of patients with COVID-19 in this hospital are basically used rationally under the guidance of the "New Coronary Virus Pneumonia Diagnosis and Treatment Program" continuously updated by the National Health Commission at the same time..

Chief Editor: LI Jie Wei

Publication Number:

ISSN 2097-2024   CN  31-2185/R

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