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Content measurement of doxorubicin and lonidamine by HPLC
SUN Yuhan, XU Ziyi, LIAO Jun, ZHANG He, FAN Li, LU Ying
, Available online  , doi: 10.12206/j.issn.2097-2024.202306043
Abstract(466) HTML (199) PDF (1034KB)(7)
  Objective  To establish a method for the simultaneous determination of doxorubicin (DOX) and Lonidamine (LND).   Methods  HPLC was performed on Agilent 5 HC-C18(2) (4.6 mm × 250 mm, 5 µm) column. The mobile phase was methanol-0.1% TFA aqueous solution, and the gradient elution procedure was 0 to 3 min, 65% methanol; 3 to 7 min, 65%→90% methanol; 7 to 13 min, 90% methanol; 13 to 15 min, 90%→65% methanol; 15 to 20 min, 65% methanol. The collection time is 20 min, the balance time is 3 min, the UV detection wavelengths were 205 nm and 253 nm. The flow rate was 1.0 ml/min and the column temperature was 35℃. The amount of inlet is 10 µl.   Results  The method was highly specific, and both DOX exhibited good linearity in the concentration range of 1-40 µg/ml and 6-240 µg/ml, respectively. The two compounds’ precision, stability, and recovery satisfied the requirements of the method.   Conclusion  This study established an HPLC method that was suitable for the simultaneous detection of DOX and LND. This method’s high level of specificity, accuracy, and reliability could lead to quantitative detection.
Observation of the prophylactic use of prokinetic agents in patients after tricuspid valve replacement under cardiopulmonary bypass
CHEN Qian, XU Xiangyang, LU Fanglin, HAN Lin, LI Bailing, XU Zhiyun
, Available online  , doi: 10.12206/j.issn.2097-2024.202210060
Abstract(252) HTML (92) PDF (837KB)(2)
  Objective  To analyze the postoperative therapeutic effect of prokinetic agents in patients after tricuspid valve replacement (TVR) under cardiopulmonary bypass.   Methods  Patients received TVR under cardiopulmonary bypass (during June 2010 to December 2021) in the department of Cardiovascular Surgery of the First Affiliated Hospital of Naval Medical University were selected as the subjects of our study. The data of basic characteristics, first postoperative defecation time and postoperative recovery condition were collected and retrospectively analyzed in the patients taking prokinetic agents within three days after surgery (prevention group) and patients not taking prokinetic agents within three days after surgery (control group).   Results  A total of 184 patients were selected, including 101 in the prevention group and 83 in the control group. The first defecation time of patients in the prevention group was significantly earlier than that in the control group (P<0.05). The incidences of abdominal distension and pulmonary infection in the prevention group were significantly lower than that in the control group. The length of time in ICU, postoperative mechanical ventilation and nasogastric tube decompression in the prevention group were significantly shorter than that in the control group (P<0.05).   Conclusion  Taking prokinetic agents within three days after TVR under cardiopulmonary bypass could effectively improve the prognosis of patients.
The protective effect of Rongmu Fugan Formula on ANIT induced liver injury in mice with cholestasis
YANG Nian, YANG Liu, ZHENG Sijia, ZHANG Junxia, XU Huaming
, Available online  , doi: 10.12206/j.issn.2097-2024.202305008
  Objective  To explore the protective effect of Rongmu Fugan Formula on ANIT induced cholestasis liver injury in mice.   Methods  8-week old SPF Kunming mice were randomly divided into 6 groups, 10 mice in each group which were the blank control group (normal saline gavage), the model control group (normal saline gavage), the low (gavage dose:5.25 g/kg ) group, the medium (11.25 g/kg) group and the high (20.25 g/kg) group of Rongmu Fugan Formula, the positive control (Ursodeoxycholic acid, UDCA,0.1 g/kg). Mice were administered with continuous gavage once a day, for 7 consecutive days. On the 5th day, the blank normal control group was given an equal amount of olive oil by gavage, all other groups were given ANIT (65 mg/kg) by gavage for modeling. After 48 hours of modeling, 30 minutes after the last administration of each group of mice, blood and liver tissue samples were collected for testing of various indicators. The corresponding kits were used to detect the content of alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), total bile acid (TBA),γ-glutamyl transpeptidase(γ-GT), total bilirubin (TBIL), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) in peripheral blood of mice, respectively. HE staining was used to observe the pathological changes in the liver of mice.   Results  Compared with the model group, each dose group of Rongmu Fugan Formula reduced the levels of ALT, AST, AKP, TBA, TBIL,γ-GT and MDA (P<0.05) significantly, markedly increased the levels of SOD and GSH-PX (P<0.05). Compared with the model group, the low-dose and high-dose groups of Rongmu Fugan Formula showed a decrease in liver cell vacuolar degeneration and reduction in inflammatory infiltration. In the medium dose group, the vacuolar degeneration of liver cells was significantly reduced, the inflammatory infiltration was significantly improved, the liver cells were arranged in cords, and the structure of lobules of liver was intact.   Conclusion  Rongmu Fugan Formula could improve serum biochemical indicators of liver injury, reduce jaundice and eliminate oxidative free radicals, thereby exerting a protective effect against ANIT-induced cholestatic liver injury in mice.
Research progress on targeted therapy and immunotherapy for esophageal cancer
MA Zifen, XU Weiheng, JIN Yuxiang, XUE Lei
, Available online  , doi: 10.12206/j.issn.2097-2024.202306008
Esophageal cancer is a malignant tumor with high incidence and mortality rate in the world and its pathogenic factors are complex and diverse. There are no obvious symptoms in the early stage, and most patients are in the middle to late stage at the initial diagnosis. The prognosis of esophageal cancer is poor. The treatment mode of conventional surgical resection combined with chemoradiotherapy can no longer meet the current treatment needs of disease, and new treatment strategies are urgently needed. Molecular targeted therapy and immunotherapy are new treatment methods that have emerged in recent years, which have broken the therapeutic bottleneck and have been proven to play important roles in the treatment of esophageal cancer. The current research progress of the main targets and their related targeted drugs in molecular targeted therapy and immunotherapy for esophageal cancer were reviewed in this article, which provided reference for the application of precision medicine in the field of esophageal cancer.
Determination of four different components in Danggui Liuhuang decoction by HPLC/MS/MS
LI Dan, DAI Xianchun, WANG Fangzhen, LIU Xiaozhen, CHEN Yihan, HU Yating, YANG Ping, LIU Jiyong
, Available online  , doi: 10.12206/j.issn.2097-2024.202305007
  Objective  To establish the method of simultaneous determination of four main components of Danggui Liuhuang Decoction, including phellodendrine, palmatine, calycosin, and ferulic acid and provide reference for the quality control of Danggui Liuhuang Decoction.   Methods  Based on the HPLC-MS/MS analysis method, the positive ion data acquisition mode was adopted for the mass spectrometry detection and the four main components were quantified with multiple reaction monitoring mode (MRM) by ESI source. The chromatographic column was Agilent Extend-C18 (5 μm, 4.6 mm×250 mm), and gradient elution was performed with methanol and 0.5% formic acid in water.   Results  The linear range of phellodendrine was from 2-200 nmol/ml, and the linear range of palmatine, calycosin and ferulic acid was from 20-2 000 nmol/ml. The contents of the four components in the seven batches of Danggui Liuhuang Decoction were relatively stable, among which ferulic acid was mainly found in Phellodendrine and Coptidis; Phellodendrine was only detected in cortex phellodendri; the content of calycosin in Scutellaria baicalensis and Astragalus was higher; palmatine was detected in both Phellodendron and Astragalus.   Conclusion  The method has high sensitivity, good specificity and sample stability, which could meet the requirements of quantitative analysis of Traditional Chinese Medicine compounds, and could provide reference for further pharmacokinetics study on the content changes of traditional Chinese medicine compounds in biological samples.
Research progress on the regulation of intestinal flora and effect on intestinal absorption and transport by TCM components
JIANG Jun, CHEN Li, LI Zhong, LIU Jinge, QI Tian, ZHOU Tingting
, Available online  , doi: 10.12206/j.issn.2097-2024.202303012
  Objective  To introduce the research progress on the regulation of intestinal microbiota by Traditional Chinese Medicine (TCM) components and their effect on intestinal absorption and transportation, in order to provide assistance for future clinical rational drug use targeting intestinal microbiota.   Method  The literature on intestinal microbiota and intestinal absorption and transportation in recent years were analyzed and summarized, and the mechanism of the regulation of intestinal microbiota by traditional Chinese medicine ingredients on drug absorption and transportation were elaborated.   Result  The ingredients of traditional Chinese medicine alter the gut microbiota, thereby affecting the intestinal barrier function and absorption of transport proteins.   Conclusion   It is great significance for clinical rational drug use to study the effects of TCM ingredients on the regulation of absorption and transportation of intestinal microbiota.
Study of the treatment compliance and influencing factors of inhalation therapy in patients with stable chronic obstructive pulmonary disease
DUAN Hu, ZHOU Yanqing, QIAN Yulin, ZHAO Liang
, Available online  , doi: 10.12206/j.issn.2097-2024.202204094
Abstract(470) HTML (264) PDF (839KB)(14)
  Objective  To study the compliance and influencing factors of inhalation therapy in patients with stable chronic obstructive pulmonary disease (COPD),and carry out patient education and management accordingly.   Methods  COPD patients were selected from Respiratory Clinic of Luodian Hospital of Baoshan District of Shanghai from June to December of 2021. Compliance and inhalation techniques were assessed with the Morisky Medication Adherence Scale and the ten-step inhalation technique, and the factors influencing compliance were analyzed.   Results  A total of 58 outpatients with COPD were included, in which 25 cases (43.1%) with moderate or above compliance. Univariate analysis showed that the patients with course of disease ≥5 years, CAT(COPD assessment test)≥10 points, used 2 inhalation devices and inhalation technique score ≥8 points had better compliance when compared with other patients (P<0.05).  Conclusion  Patient education and management should be carried out actively. The patients with course of disease<5 years, CAT<10 points should be highly concerned. The patients' ability to use inhalation devices and personal preference should be fully considered. Training on the use of devices should be strengthened and regularly follow-up evaluation should be carried out.
Analysis of the chemical constituents of Maxing Shigan decoction by UPLC-Q-TOF/MS
ZHAO Xue, GU Yanqiu, CHU Haowen, WU Caisheng, LI Gao, CHEN Xiaofei
, Available online  , doi: 10.12206/j.issn.2097-2024.202306028
Abstract(529) HTML (174) PDF (1510KB)(7)
  Objective  To analyze chemical constituents of compound Maxing Shigan decoction by ultra-high perfor-mance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS).   Methods  The separation was performed on a UPLC BEH C18 column (2.1 mm×100 mm, 2.5 µm), with a gradient elution applying 0.1% aqueous formic acid solution and 0.1% formic acid acetonitrile as a mobile phase. The column temperature was 40 °C. The flow rate was 0.4 ml/min and the analysis time was 15 min. Mass spectrometry (MS) data were collected in both positive and negative ESI ion modes.   Results  Through UPLC-QTOF/MS analysis and reference validation, a total of 59 chemical components in Maxing Shigan decoction were identified.   Conclusion  In this study, an ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS) method was established to identify the chemical components of Maxing Shigan decoction. This method is simple, efficient, sensitive and accurate, and provides a basis for the elucidation of the pharmacodynamic material basis and mechanism of Maxing Shigan decoction. It can provide data reference for the optimization of the compatibility of traditional Chinese medicine in the treatment of COVID-19.
Molecular mechanism of Shenling Baizhu powder in treatment of cancer cachexia based on network pharmacology
KE Gang, DONG Qingke, XIAO Shirong, GONG Qian, LI Rong, WANG Daijie
, Available online  , doi: 10.12206/j.issn.2097-2024.202208115
Abstract(461) HTML (196) PDF (3143KB)(10)
  Objective   To analyze the pharmacological mechanism of Shenling Baizhu powder in the treatment of cancer cachexia based on the network pharmacological method and provide a reference for the clinical application of classical traditional Chinese medicine(TCM) prescriptions.   Methods   Through TCMSP and BATMAN-TCM databases, the main chemical components and their targets of the TCM prescription of Shenling Baizhu powder were obtained, and the active components of the TCM were screened according to ADME. The main targets of cancer cachexia were obtained through OMIM, Genecards, Disgenet and DRUGBANK databases, and protein interaction analysis was conducted using String platform to build a PPI network. The “drug active ingredient target” network of Shenling Baizhu powder was constructed by Cytoscape 3.7.2 software, and then the biological processes and pathways involved were analyzed by using Metascape platform. Finally, molecular docking verification was conducted by Discovery Studio.   Results  The core active ingredients of Shenling Baizhu powder in the treatment of cancer cachexia are quercetin, kaempferol, pyrolignous acid, stigmasterol, luteolin, β-sitosterol, etc. The core targets are AKT1, TP53, TNF, IL-6, MAPK3, CASP3, JUN, CTNNB1, HIF1A, EGFR, etc. The molecular docking test also showed that the top 10 active ingredients, such as pyrolignous acid, stigmasterol and β-sitosterol, had good binding activities with most of the target sites. The biological pathway of Shenling Baizhu powder in treating cancer cachexia is mainly to regulate tumor related pathway, metabolism related pathway, inflammatory factors and appetite related pathway.  Conclusion  This study preliminarily revealed the mechanism of action of Shenling Baizhu powder in treating cancer cachexia with multi components, multi targets and multi pathways, which provided a basis for the clinical development and utilization of Shenling Baizhu powder.
Research progress on natural medicines and active compounds in the treatment of heart failure
WU Xinyi, WAN Jingjing, LIU Xia
, Available online  , doi: 10.12206/j.issn.2097-2024.202306013
Heart failure is the terminal stage of various cardiovascular diseases and a leading cause of death. For a long time, natural medicines have been used to treat heart failure with remarkable effects. In this paper, the Traditional Chinese Medicine compound patents in the national patent database were mined, common Traditional Chinese Medicines for the clinical treatment of HF were selected, and the single active ingredient contained in them was analyzed, which provided some valuable tips for the development of drugs for the treatment of heart failure.
The mechanism of nicotinamide combined with amphotericin B against Candida albicans based on metabolomics technology
WAN Lizhi, WANG Jinghan, WU Chunrong, LI Ling
, Available online  , doi: 10.12206/j.issn.2097-2024.202307034
  Objective   To investigate the potential mechanism of nicotinamide combined with amphotericin B against Candida albicans based on metabolomics.   Methods  The intracellular metabolites of C. albicans intervened by different drugs including NAM, AmB, and their combination with a proper concentration were analyzed by gas chromatography-mass spectrometry. The differential metabolites were screened by multivariate statistical analysis and identified by searching the NIST database.   Results   Compared with the control group, the NAM intervention group was hardly separated from it, while the AmB group and NAM+AmB group showed a clear trend separation. Under the intervention of AmB, 23 metabolites significantly changed compared with the control group, and 28 metabolites remarkably changed after NAM+AmB intervention, including amino acids, organic acids, sugars and other components.   Conclusion  NAM, as an endogenous metabolite of C. albicans, combined with AmB could enhance the effects of AmB in the original metabolic pathway and changed it to a certain extent. It is speculated that AmB combined with NAM may pose more antifungal effect on Candida albicans by regulating the tricarboxylic acid cycle, interfering with amino acid metabolism and influencing polyamine synthesis.
Pharmacoeconomic evaluation of pembrolizumab versus platinum chemotherapy as first-line treatment in advanced non-small cell lung cancer
SONG Yutong, XIA Derun, GU Heng, TANG Shaowen, YI Honggang, Wo Hongmei
, Available online  , doi: 10.12206/j.issn.2097-2024.202303023
Abstract(221) HTML (152) PDF (1382KB)(1)
  Objective  To make the cost-effectiveness analysis of pembrolizumab and platinum chemotherapy as the first-line treatment for advanced non-small cell lung cancer (NSCLC) in the population with tumor proportion score (TPS) ≥ 1% of PD-L1, and to provide some reference for the clinical use and future price negotiation of pembrolizumab.   Methods  Based on Pubmed database, the published RCT literatures of pembrolizumab were analyzed, and the survival data were extracted, combined with the treatment plan of Jiangsu Province Hospital, the Markov model were established to simulate the cost and health effectiveness of patients for twenty years, and the stability of the model was tested by one-way sensitivity analysis and probability sensitivity analysis.   Results  Twenty years later, the cost-effectiveness ratio of pembrolizumab group and chemotherapy group was ¥58 517.60/QALM (quality adjusted life month), ¥41 213.08/QALM. Compared with the chemotherapy group, the incremental cost effective ratio (ICER) was ¥104 485.36/QALM.   Conclusion  When the willingness to pay (WTP) value was ¥30 902/ QALM, the pembrolizumab therapy is not more cost-effective advantages than platinum chemotherapy, and the sensitivity analysis showed that the results of the model were relatively stable.
Advances in chiral analysis and stereoselective pharmacokinetics of dihydropyridine calcium antagonists
ZHANG Yixin, GUAN Xinyi, WANG Boning, WEN Jun, HONG Zhanying
, Available online  , doi: 10.12206/j.issn.2097-2024.202308062
Abstract(342) HTML (153) PDF (1024KB)(2)
[Abstract] Chiral drugs are closely related to the safety and effectiveness of drug use. Most dihydropyridine drugs have chiral carbon atoms, which are used as racemes and produce stereoselective disposal characteristics after entering the body, which may affect the safety and effectiveness of drugs. Therefore, based on the chiral resolution and pharmacokinetic characteristics of this class of drugs, the selection rules of chiral HPLC and CE methods and in vivo analysis applications of this class of drugs in recent years were reviewed. The stereoselective pharmacokinetics of this class of drugs were listed and compared. It was found that some of these drugs had obvious differences in stereoselective pharmacokinetics, and the pharmacokinetics and toxicity in vivo were also different.
Mechanisms of Shexiang Baoxin Pill in Homo-therapy for Heteropathy in type 2 diabetes mellitus and coronary heart disease based on network pharmacology
ZHU Zifan, WANG Zhicong, XIE Bin, LIU Runhui
, Available online  , doi: 10.12206/j.issn.2097-2024.202305037
Abstract(358) HTML (165) PDF (1851KB)(2)
  Objective  To probe into the mechanism of Shexiang Baoxin Pill in Homo-therapy for Heteropathy” for type 2 diabetes mellitus (T2DM) and coronary heart disease (CHD) based on network pharmacology.   Methods  All chemical components and action targets of these seven traditional Chinese medical in Shexiang Baoxin Pill were screened by Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP), Traditional Chinese Medicines Integrated Database (TCMID), The Encyclopedia of Traditional Chinese Medicine (ETCM) and BATMAN-TCM platform, and the DisGeNET and GeneCards databases were used to obtain CHD and T2DM-related Disease targets. The “drug-component-target” network map was constructed by Cytoscape 3.8.2 software, the protein-protein interaction (PPI) network map was constructed by STRING database, and the GO biological process analysis and KEGG pathway enrichment analysis were performed on the common targets of Shexiang Baoxin Pill for T2DM and CHD using DAVID online database.   Results  A total of 111 potential active ingredients for the treatment of T2DM and CHD in Shexiang Baoxin Pills were screened out, corresponding to 229 targets. Network analysis results showed that the common main active ingredients in Shexiang Baoxin Pills for treating T2DM and CHD might be chenodeoxycholic acid, ursodeoxycholic acid, cinnamic aldehyde, bile acids, cinnamic acid, and ginsenosides. The results of pathway enrichment analysis showed that the mechanism of action of Shexiang Baoxin Pills in the treatment of type 2 diabetes and coronary heart disease in treating T2DM and CHD might be related to the inhibition of inflammatory response and oxidative stress.   Conclusion  Shexiang Baoxin Pill could play a role in treating CHD and T2DM through multiple components, multiple targets and multiple pathways, which provided a certain theoretical basis for the clinical application and further research of Shexiang Baoxin Pills.
Research Progress on Hepatic Macrophages and Liver Fibrosis
JIANG Haiyan, ZHONG Fangfang, ZHANG Junping
, Available online  , doi: 10.12206/j.issn.2097-2024.202209030
Abstract(484) HTML (188) PDF (1028KB)(7)
Liver fibrosis is a repair response to chronic liver injury caused by various etiologies. Its continuous progression can develop into liver cirrhosis or even hepatocellular carcinoma, eventually leading to liver failure. Currently, there is no effective treatment for liver fibrosis. Hepatic macrophages play a key role in intrahepatic inflammatory response, progression and resolution of fibrosis, and have emerged as an important therapeutic target for anti-hepatic fibrosis. The function of hepatic macrophages in the process of liver fibrosis was mainly reviewed and the mode of action of hepatic macrophages from various aspects was discussed to provide ideas for the treatment of liver fibrosis.
Preparation and pharmacokinetics of flumazenil sublingual tablet
ZHANG Yingnan, HOU Cheng, XU Ziyi, LU Guangzhao, LU Ying, ZHANG He
, Available online  , doi: 10.12206/j.issn.2097-2024.202305038
Abstract(280) HTML (115) PDF (1467KB)(2)
  Objective  To prepare flumazenil sublingual tablets and study its bioavailability.   Methods  Flumazenil sublingual tablets were prepared by compressing flumazenil inclusion compound with hydroxypropyl-β-cyclodextrin as the inclusion material. In a double-cycle crossover trial, twelve beagle dogs were randomly divided into two groups, one group receiving flumazenil sublingual tablets and the other receiving flumazenil injections. LC-MS method was developed and validated to determine flumazenil plasma concentration. The pharmacokinetic parameters and bioavailability were calculated using WinNonlin pharmacokinetic software.   Results  In the pharmacokinetic study, AUClast of flumazenil injection and sublingual tablet was (8.41±2.15) and (8.86±2.83) h·ng·ml−1, respectively; cmax was (10.96±2.62) and (6.36±2.14) ng/ml, respectively; tmax was (0.18±0.05) and (0.58±0.24) h, respectively. The bioavailability of flumazenil sublingual tablet was 52.68%.   Conclusion  Clathrates were used to prepare flumazenil sublingual tablets to achieve safe and efficient delivery. LC-MS method was established for the determination of flumazenil plasma concentration, which is simple, accurate and sensitive.
The improvement of gefitinib-resistance of PC9/GR by thapsigargin combined with gefitinib
DU Jiangyuan, ZHANG Lanlin, CAI Tongkai, CAO Yongbing
, Available online  , doi: 10.12206/j.issn.2097-2024.202209018
Abstract(785) HTML (237) PDF (1785KB)(10)
  Objective  To study the effect and mechanism of the thapsigargin combined with gefitinib on the proliferation of human lung adenocarcinoma gefitinib resistance cell line PC9/GR.   Methods  The cell viability of PC9/GR treated with gefitinib alone or gefitinib combined with thapsigargin was evaluated by CCK8 assay. The flow cytometry was used to analyze the PC9/GR cell apoptosis indued by the two group drugs. The ATF-6 and IRE1α protein expression of PC9/GR cells treated with the two group drugs were detected by Western blotting.   Results  The group of drug combination exhibited enhanced ability to inhibit cell proliferation, promote cell apoptosis and upregulate the ATF-6 and IRE1α protein expression of the PC9/GR compared with the group gefitinib used alone.   Conclusion  The sensitivity of PC9/GR to gefitinib was increased when the cells were treated by thapsigargin, which may be related with the state of endoplasmic reticulum stress(ERS) induced by thapsigargin.
, Available online  
Abstract(375) HTML (152) PDF (699KB)(19)
, Available online  , doi: 10.12206/j.issn.2097-2024.
Abstract(196) HTML (92)
Preparation and dissolution behaviors in vitro evaluation of silymarin phospholipid complex
ZENG Qiping, LIU Jianqing, YANG Li'na, SONG Hongtao
, Available online  , doi: 10.12206/j.issn.2097-2024.202206051
Abstract(524) HTML (274) PDF (3056KB)(7)
  Objective  To prepare silymarin phospholipids complex(SM-PC) and investigate its physicochemical properties.   Methods  On the basis of single-factor tests, the drug-lipid ratio, drug concentration and reaction temperature were selected as the factors of the central composite design and response surface methodology in the preparation of SM-PC by solvent volatilization, and the best process was optimized with the compound rate as the index. And its dissolution in vitro was measured.   Results  The optimum preparation technology of SM-PC was as follows: acetone was used as compound solvent; the concentration of SM was 8.0 mg/ml; the mass ratio of SM to phospholipid was 1∶1.8; the reaction temperature was 56 ℃ and the recombination rate was(95.15±1.55)% with deviation of less than 3%. The in vitro dissolution test showed that the dissolution of SM-PC was close to 90% in 60 min. The dissolution behavior of main component of silybin was similar to that of silymarin capsules(Legalon ®), which was higher than SM-API.   Conclusion  SM-PC was successfully prepared by central composite design response surface method, which significantly improved the dissolution and laid a foundation for the study of subsequent preparations.
Participation of Clinical pharmacists in a case of QTc interval prolongation induced by Gilteritinib
CUI Xiaolin, FU Xiaofei, DU Yanhong, LIU Juan, ZHU Qian, LIU Ziqi
, Available online  , doi: 10.12206/j.issn.2097-2024.202309050
  Objective  To explore the role of clinical pharmacists involved in the case of a patient with acute myeloid leukemia whose QTc interval prolongation was induced by Gilteritinib, and to provide reference for drug treatment and monitoring of those patients.   Methods  the abnormal electrocardiogram(ECG) of a patient with acute myeloid leukemia was found in time by clinical pharmacists, who participated in clinical diagnosis and treatment by analyzing the patient's underlying diseases, diagnosis and treatment process, therapeutic drugs and their potential interactions.   Results  Clinical pharmacists suspected that the prolonged QTc interval was likely to be an adverse reaction caused by Gilteritinib, and recommended immediate discontinuation of the drug and re-examination of the electrocardiogram.The physician took the suggestion to stop the suspected drug therapy with Gilteritinib promptly, and ECG was rechecked three days later, and the QTc value returned to the normal range.   Conclusion  Clinical pharmacists participating in clinical diagnosis and treatment could provide better pharmaceutical care for patients.
Research progress on the pharmacological effects of maslinic acid
ZHANG Linchen, ZHANG Xiaoqin, ZHANG Junping
, Available online  , doi: 10.12206/j.issn.2097-2024.202307052
Abstract(456) HTML (253) PDF (896KB)(4)
Maslinic acid is a pentacyclic triterpenoid that is widely found in natural plants such as olives and hawthorns. Maslinic acid has anti-tumor, anti-inflammation, anti-oxidation, antibacterial, cardiovascular protection, neuroprotection, and other pharmacological effects. In this paper, the research progress of pharmacological activities and the mechanism of action of maslinic acid were reviewed, which provides the basis for the development and utilization of maslinic acid in the future.
Lactic acid bacteria as recombinant protein drug delivery carrier
WANG Xufang, WANG Yanting, YAO Chunmeng, LU Bin
, Available online  , doi: 10.12206/j.issn.2097-2024.202309021
Abstract(555) HTML (169) PDF (863KB)(10)
Lactic acid bacteria is a good candidate in living drug delivery system for its safety, beneficial nature, and intestinal colonizability. At present, most studies use it as a protein drug delivery carrier for disease treatment. As a model organism, a variety of gene modification schemes enable it to be applied to various diseases and can play a significant therapeutic effect. Lactic acid bacteria drug carrier has many advantages, including non-invasive drug delivery, gene editing, large-scale production Therefore, the use of lactic acid bacteria as recombinant protein expression vector has attracted global attention. In this review, the application basis, bioavailability improvement, gene editing strategy and research and application status of lactobacillus drug delivery system were summarized.
Study on the effect of Lishukang capsule on learning and memory impairment in mice with high altitude hypoxia based on Keap1/Nrf2/HO-1 signal pathway
MENG Panpan, SONG Muge, YANG Shichao, CHEN Keming, YANG Zhongduo, MA Huiping
, Available online  , doi: 10.12206/j.issn.2097-2024.202303006
Abstract(782) HTML (232) PDF (2083KB)(13)
  Objective  Study on the effect of Lishukang capsule on learning and memory impairment in mice with high altitude hypoxia based on Keap1/Nrf2/HO-1 signal pathway.   Methods  Sixty male Balb/C mice were randomly divided into normal control group, hypoxia model group, Rhodiola capsule group: 400 mg/kg, low, medium and high dose groups of Lishukang capsule: 400 mg/kg, 600 mg/kg, 800 mg/kg, with 10 mice in each group. The normal control group was fed at the local altitude (1500m) after 7 days of intragastric administration in each group, and the rest groups were fed at the low pressure and hypoxia animal experimental cabin to simulate the altitude of 7500 m for hypoxia for 3 days. During this period, the normal control group and the hypoxia model group were given normal saline once a day, and 1 hour after the last administration, the eight arm maze was used to test the spatial memory ability of mice under simulated high altitude hypoxia; HE staining was used to observe the morphological changes of hippocampus in mice; Western blot was used to detect the changes of protein content of Keap1/Nrf2/HO-1 signal pathway and apoptosis related protein in hippocampus of mice.   Results  Compared with the normal control group, the spatial memory ability of mice in the hypoxia model group was significantly impaired (P<0.01); HE staining showed that hippocampal neurons in mice were seriously injured; the content of brain tissue Keap1 protein and apoptosis related protein Bax and Caspase-3 increased (P<0.01); the content of Nrf2, HO-1 and apoptosis related protein Bcl-2 decreased (P<0.01). Compared with the hypoxia model group, the error rate of mice in the high dose group of Lishukang capsule in the eight arm maze behavior experiment was significantly reduced (P<0.05, P<0.01); HE staining showed that the neurons were arranged orderly and the cell morphology was good; the content of Keap1 protein and apoptosis related protein Bax and Caspase-3 decreased (P<0.01); the content of Nrf2, HO-1 and apoptosis related protein Bcl-2 increased (P<0.01).   Conclusion  High altitude hypoxia can lead to oxidative stress injury in mice and induce the expression of apoptosis related genes, thus aggravating the cognitive dysfunction of mice; Lishukang capsule can effectively improve the learning and memory impairment in mice caused by hypoxia, and its mechanism may be related to regulating the Keap1/Nrf2/HO-1 signal pathway and reducing apoptosis.
Anti-fatigue activity of selenium nanoparticles functionalized by polysaccharides from Pleurotus tuber-regium sclerotium
ZOU Si, WU Yanbin, WU Jinzhong, WU Jianguo, WONG Kahing
, Available online  , doi: 10.12206/j.issn.2097-2024.202206072
Abstract(630) HTML (290) PDF (1745KB)(9)
  Objective  To investigate the anti-fatigue effect of PTR-SeNPs in vivo by measuring the muscle relative length of hindlimb, load-bearing swimming time as well as serum and liver indexes of mice.   Methods  48 Male C57/BL6 mice were randomly assigned into 4 groups with 12 mice in each group, including vehicle control group (Control group), swimming training exercise group (EC group) with vehicle treatment, swimming training exercise with low dose of PTR-SeNPs group (LPTR-SeNPs) and high dose of PTR-SeNPs group (HPTR-SeNPs). The mice were intragastrically administrated with normal saline in both Control group and EC group, as well as 2.5 and 10 μmol/kg·bw PTR-SeNPs in L/HPTR-SeNPs group, respectively, once per day for consecutively 21 days. After swimming training exercise, the muscle structures in the hind limb of mice were examined by magnetic resonance imaging. Furthermore, the burdened swimming time was measured, the serum content of blood lactic acid (BLA), urea nitrogen (BUN), alanine aminotransferase (ALT), glutamic oxalate aminotransferase (AST) and lactate dehydrogenase (LDH), as well as the hepatic level of glycogen (HG), malondialdehyde (MDA) and activity of catalase (CAT) and superoxide dismutase (SOD) were determined.   Results  Compared with the Control group, the serum contents of BLA, BUN, ALT, AST and LDH in EC group (P<0.05 or 0.01) and hepatic CAT in HPTR-SeNPs group (P<0.01) were significantly increased. The muscle relative length of hind limbs and the burdened swimming time were extended by HPTR-SeNPs markedly (P<0.05). There was no significant difference in MDA level in L/HPTR-SeNPs group. Compared with EC group, the burdened swimming time of mice was significantly prolonged (P<0.01), the contents of BLA and BUN were obviously decreased in the HPTR-SeNPs group(P<0.05 or 0.01), the level of HG was significantly increased in the L/HPTR-SeNPs groups (P<0.05 or 0.01), the serum content of ALT, AST and LDH were markedly decreased in the HPTR-SeNPs group (P<0.05 or 0.01). Hepatic SOD activity was remarkably increased in LPTR-SeNPs group (P<0.05), the level of CAT was evidently increased (P<0.01) and AST was decreased (P<0.05) in the HPTR-SeNPs group.   Conclusion  PTR-SeNPs could improve the liver physiological function, increase glycogen storage, reduce the accumulation of metabolites and enhance the body’s antioxidant capacity to ameliorate fatigue significantly, which could present the potential to be developed into health care products or drugs.
Determination and effect of five alkaloids from extracts of Piper longum on rats with experimental myocardial ischemia induced by injection of pituitrin
YI Bowen, LIU Huining, ZHENG Rui, REN Jiawei, LIU Yang
, Available online  , doi: 10.12206/j.issn.2097-2024.202112011
Abstract(1187) HTML (384) PDF (999KB)(14)
  Objective  To determine the content of five alkaloids from extracts of piper longum and test the pharmacodynamic effect of them on rats with experimental myocardial ischemia induced by injection of pituitrin.   Methods  The content of five alkaloids was determined simultaneously by HPLC. The experimental myocardial ischemia in rats was induced by injection of pituitrin, and the absolute value of T wave change and change of heart rate before and after model establishment were chosen to be the observation index. The effects of large, medium and small dose groups were evaluated.   Results  Three batches of samples were analyzed, with the contents of piperine for 56.1%, 49.7%, 51.6%; N-isobutyl-(2E,4E)octadecatrienamide for 4.5%, 4.2%, 4.3%; guineensine for 0.46%, 0.38%, 0.40%; piplartine for 1.73%, 1.67%, 1.70% and piperamide for 0.55%, 0.46%, 0.49%, respectively. All dose groups from extracts of piper longum had significantly reduced the absolute value of T wave and almost have no effect on the change of heart rate, except the high dose group showed the effect of reducing heart rate at some time .  Conclusion  The HPLC method was suitable for the simultaneous determination of five alkaloids from extracts of piper longum. It was shown that extracts of piper longum had good bioactivity in anti-myocardial ischemia.
, Available online  
Abstract(3303) HTML (1611) PDF (663KB)(281)