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2024, Volume 42,  Issue 7

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Research progress on quantitative detection methods of lateral flow immunochromatography assay
GU Jiayu, HU Xiner, WANG Xiaofei, ZHANG Ying, ZHANG Hai, CAO Yan
2024, 42(7): 273-277, 284. doi: 10.12206/j.issn.2097-2024.202307037
Abstract(789) HTML (441) PDF (1282KB)(39)
Lateral flow immunochromatography assay is a new instantaneous detection technology that employs a chromatographic membrane and labeling materials for detection. This detection technology is convenient, fast, and inexpensive, and is therefore widely used in a number of different fields, such as biomedicine, disease detection, food safety, environmental protection, and so on. Traditional lateral flow immunochromatography assay relied on visual observation and provided only qualitative or semi-quantitative results. By utilizing various types of markers and sensitive detection devices, lateral flow immunochromatography assay enables quantitative and multi-component detection of the analytes. The research progress on the lateral flow immunoassay detection system and its current applications in the context of recognition elements, labeling materials, and detection instruments were reviewed in this paper.
Progress on pharmaceutical characteristics of potassium-competitive acid blocker
WANG Yaozhen, XU Can, LV Shunli, TIAN Jing, ZHANG Dongwei
2024, 42(7): 278-284. doi: 10.12206/j.issn.2097-2024.202306040
Abstract(1970) HTML (512) PDF (1004KB)(46)
As a new class of acid inhibitors, potassium-competitive acid blocker(P-CAB) inhibits the conformational transition of H+, K+-ATPase with subsequent suppression of H+, K+ exchanging by binding reversibly near the K+ binding site of H+, K+-ATPase, which results in the inhibition of gastric acid secretion in a K+-competitive manner. The unique structure and novel mechanism of P-CAB contribute to the pharmaceutical characteristics superior to other PPIs, making it a new alternative for acid-related diseases(ARDs). Progress on pharmaceutical characteristics of P-CAB were reviewed in this paper.
The roles of Bajitianwan formula on Aβ-injured osteoblasts and the mechanism based on network pharmacology
JIANG Tao, XU Weifan, JIANG Yiping, XIA Tianshuang, XIN Hailiang
2024, 42(7): 285-290, 296. doi: 10.12206/j.issn.2097-2024.202305011
Abstract(1413) HTML (250) PDF (2487KB)(13)
  Objective  To explore the effect of Bajitianwan(BJTW)formula on bone formation of Aβ-injured osteoblasts and its mechanism.   Methods  Osteoblasts isolated from neonatal 24-hour Wistar rats were used for the study, and osteoblasts were subjected to damage with Aβ1-42 oligomers, and pharmacological intervention was performed with the aqueous extract of BJTW formula. The MTT assay, alkaline phosphatase(ALP)activity assay, catalase(CAT)activity assay, superoxide dismutase(SOD)activity assay, glutathione(GSH)activity assay and malondialdehyde(MDA)activity assay were carried out respectively. The expression levels of bone morphogenetic protein 2(BMP2), osteogenic specific transcription factor(RUNX-2)and osteoprotective protein(OPG)were detected by Western blotting. After confirming the effect of BJTW formula on Aβ-injured osteoblasts, the network pharmacology method was used to predict the potential pathways.   Results  The BJTW formula significantly promoted the proliferation of Aβ-injured osteoblasts, increased ALP, SOD and GSH activity, inhibited MDA activity, and promoted the expression of bone formation-related proteins BMP2, RUNX-2 and OPG. Network pharmacological analysis showed that the effect of ameliorating of Aβ-injured osteoblasts by BJTW formula was mainly mediated by AGE-RAGE, PI3K-Akt, MAPK and neuroactive ligand-receptor interaction signaling pathways.   Conclusion  In this study, the effect of BJTW formula on improving the osteoblasts damaged by Aβ was confirmed for the first time, and its related mechanism was explored based on network pharmacology method. The results lay a strong foundation for the clinical application of traditional formula BJTW against osteoporosis.
Exploration of the role and mechanism of all-trans retinoic acid on activation and oxidative stress of hepatic stellate cell
XIU Jianping, YANG Chaoai, LIU Xi’ao, PAN Qianyu, WEI Guangxu, WANG Weixing
2024, 42(7): 291-296. doi: 10.12206/j.issn.2097-2024.202312054
Abstract(323) HTML (150) PDF (1501KB)(13)
  Objective  To explore the role and potential mechanisms of all-trans retinoic acid (ATRA) on activation and oxidative stress of hepatic stellate cell (HSC).   Methods  Platelet-derived growth factor (PDGF-bb, 10 ng/ml) was applied to induce the activation of HSCs, which was then treated with ATRA at a dosage of 5 μmol/L for 48 h. The effects of ATRA on HSC activation were evaluated by detecting changes in cell growth viability and phenotypic marker expression. The effects of ATRA on HSC oxidative stress were evaluated by detecting changes in intracellular reactive oxygen species (ROS), reduced glutathione (GSH) and malondialdehyde (MDA), and the expression of antioxidant genes. The effects of ATRA on HSC autophagic activity were evaluated by detecting changes in autophagy marker expression and autophagic flow.   Results  Compared with the PDGF-bb group, the cell viability was significantly reduced in ATRA-treated HSCs (P<0.01), as well as the expression of α-SMA and Collagen I. The intracellular levels of ROS and MDA were significantly reduced in ATRA-treated HSCs (P<0.01), whereas the GSH level was significantly increased (P<0.01). The expression levels of antioxidant genes (NRF2, HO-1, and ATF4), were significantly higher in ATRA-treated HSCs than those in the normal ones under PDGF-bb condition (P<0.01). Meanwhile, the expression of autophagy markers Beclin 1 and LC3 Ⅱ/I, and signal of autophagy flow in ATRA-treated HSCs were found to be significantly reduced (P<0.01).   Conclusion  ATRA significantly inhibited PDGF-bb-induced HSC activation and reduced the level of oxidative stress and autophagic activity of HSCs, which had potential applications in the prevention and treatment of liver fibrosis.
HPLC fingerprint of Radix Rhubarb and forbidden pesticide residues
MAO Zeling, WEN Bo
2024, 42(7): 297-304, 314. doi: 10.12206/j.issn.2097-2024.202310057
  Objective   To establish the HPLC fingerprint and pesticide residue detection methods for different kinds of rhubarb, and evaluate the quality of rhubarb comprehensively.   Methods   20 batches of three types of rhubarb were collected and analyzed by high-performance liquid chromatography. The mobile phase was methanol-0.1 % phosphoric acid solution; gradient elution; column temperature of 35 ℃; detection wavelength of 254 nm; flow rate 1.0 ml/min. And cluster analysis was performed on the results. Direct extraction method was used and high-performance liquid chromatography-tandem mass spectrometry and gas chromatography-tandem mass spectrometry were established, 33 prohibited pesticides from different sources and origins of rhubarb were detected.   Results   The similarity among the fingerprint spectra of three sources of rhubarb, namely Rheum palmatum L., Rheum tanguticum Maxim.ex Balf., and Rheum officinale Bail1., and their control fingerprint spectra was>0.95. 20 batches of rhubarb samples were divided into 3 categories by cluster analysis. 33 prohibited pesticides were detected in rhubarb samples from different regions.   Conclusion   The quality of three kinds of rhubarb was significantly different. The established HPLC fingerprint and the method of banning agricultural residues were stable, reliable, simple and accurate, which could provide a basis for quality control evaluation of rhubarb.
Effects of dulaglutide combined with metformin on body metabolism, body fat composition and serum adipokines in obese patients with type 2 diabetes mellitus
MAO Zhiyi, WANG Xiaoyan, CHEN Xiaoying, TANG Yifei
2024, 42(7): 305-309. doi: 10.12206/j.issn.2097-2024.202305032
Abstract(306) HTML (167) PDF (882KB)(3)
  Objective  To explore the clinical efficacy of dulaglutide combined with metformin in the treatment of obese patients with type 2 diabetes mellitus(T2DM).   Methods  A total of 200 obese patients with T2DM who were treated in Shanghai Jiading District Anting Hospital from January 2021 to January 2023 were randomly divided into liraglutide group(n=100)and dulaglutide group(n= 100). The liraglutide group was treated with liraglutide combined with metformin, and the dulaglutide group was treated with dulaglutide combined with metformin. Both groups were treated for 3 months. The body metabolic indexes [fasting blood glucose(FBG), 2 h postprandial blood glucose(2 h PBG), hemoglobin(HbA1 c), total cholesterol(TC), triglyceride(TG)], body fat composition [body fat rate, body mass index, subcutaneous fat rate of limbs, visceral fat index] and serum adipokines(adiponectin, neuropeptide Q(NPQ), asprosin, irisin)levels were compared before treatment and 3 months after treatment. The clinical efficacy and adverse reactions of the two groups were observed.   Results  After 3 months of treatment, FBG, 2 h PBG, HbAlc, TC, TG, body fat rate, body mass index, subcutaneous fat rate of limbs, visceral fat index and asprosin in the two groups were lower than those before treatment, and those in the dulaglutide group were lower than those in the liraglutide group(P<0.05). After 3 months of treatment, the levels of serum adiponectin, NPQ and irisin in the two groups were higher than those before treatment, and the increase in the dulaglutide group was greater than that in the liraglutide group(P<0.05). The effective rate of dulaglutide group(98.00%)was higher than that of liraglutide group(91.00 %)(P<0.05). There was no significant difference in the incidence of adverse reactions between the two groups(11.00%, 14.00%)(P>0.05).   Conclusion  Dulaglutide combined with metformin could improve the metabolic status of obese T2 DM patients, regulate body fat composition and serum adipokines, with significant clinical efficacy and safety.
Some thoughts on compulsory licensing of pharmaceutical patents in emergency situations
FEI Yonghe, CUI Lijun, CHEN Jing
2024, 42(7): 310-314. doi: 10.12206/j.issn.2097-2024.202309047
Abstract(233) HTML (103) PDF (926KB)(2)
  Objective  To explore the conditions for compulsory licensing of pharmaceutical patents and the steps for implementing compulsory licensing of pharmaceutical patents.   Methods   The current situation and problem of compulsory licensing of pharmaceutical patents in China were analyzed by collecting the relevant laws and regulations in China, United States, India, and the World Trade Organization. The feasible practices of compulsory licensing of pharmaceutical patents among the other countries and organizations were compared.   Results  China needs to improve the system of compulsory licensing of pharmaceutical patents, clarify the implementation details, strengthen policy guidance, and encourage cooperation between government and enterprises to ensure the smooth implementation in emergency situations.   Conclusion  Compulsory licensing system and implementation rules in China for pharmaceutical patents should be supplemented and improved by absorbing the useful provisions of international organizations and relevant countries, so as to achieve the pharmaceutical supply in emergency situations and meet the needs of public health.
Analysis of drug procurement model of multiple areas based on joint inventory in public hospitals
QU Wenjun, BAI Ruonan, CUI Li, ZHOU Yan
2024, 42(7): 315-318. doi: 10.12206/j.issn.2097-2024.202401002
Abstract(261) HTML (122) PDF (853KB)(5)
  Objective  To evaluate the application effect of joint inventory management method in drug procurement and control management in multiple hospital areas.   Methods  Based on the joint inventory management model, four pilot drugs were selected from a certain group hospital by reasonable data processing methods for research. The effects of the model application were compared and analyzed from aspects such as inventory cost, turnover situation, and supply situation.   Results  After applying the joint inventory management model led by the central hospital, the inventory and amount of drugs in the three pilot hospitals were significantly reduced, with the inventory reduced by 31.93% and the average inventory amount decreased by 16.23%; The inventory turnover days had significantly decreased, with the turnover days of all three branches decreasing by more than one day; The drug shortage rate had significantly decreased, with the most significant change among the pilot drugs being the doxorubicin liposome injection, which had a 6.7% decrease in the shortage rate; The comparison results of each group of data showed statistical significance (P<0.05).   Conclusion  Adopting a central hospital led joint inventory management model in multiple hospital areas could significantly improve the effectiveness of drug procurement management and inventory management, which enhanced the efficiency of hospital fund utilization.