HPLC法同时测定复方樟脑搽剂中苯酚和樟脑含量
Simultaneous determination of phenol and camphor in compound camphor liniment by HPLC
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摘要: 目的 建立同时测定复方樟脑搽剂中苯酚和樟脑含量的HPLC法。 方法 色谱柱为Kromasil-C18(150 mm×4.6 mm,5 μm);流动相为甲醇-水(55:45);检测波长287 nm;流速0.8 ml/min。 结果 苯酚和樟脑的线性范围分别在103.4~827.2 μg/ml(r=0.999 1, n=5)、205.1~1 640.8 μg/ml(r=0.999 5, n=5),平均回收率分别为99.61%(RSD=1.20%, n=9)、99.83%(RSD=1.18%, n=9)。 结论 该方法简便、准确,重复性好,适用于复方樟脑搽剂的质量控制。Abstract: Objective To establish a method for simultaneous determination of phenol and camphor in compound camphor liniment by HPLC. Methods The separation was performed on a Kromasil C18 analytical column(150 mm×4.6 mm, 5 μm)with the mobile phase consisting of methanol-water(55:45).The detection wavelength was 287 nm.The flow rate was 0.8 ml/min. Results The standard curves were linear in the range of 103.4~827.2 μg/ml for phenol (r=0.999 1, n=5) and 205.1~1 640.8μg/ml(r=0.999 5, n=5)for camphor. The average recoveries of phenol and camphor were 99.61%(RSD=1.19%, n=9) and 99.83%(RSD=1.18%, n=9). Conclusion The method was simple and accurate with a good reproducibility, and could be used as a quality control method for compound camphor liniment.
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Key words:
- high pressure liquid chromatography /
- compound camphor liniment /
- phenol /
- camphor
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