摘要:
目的 建立高效液相色谱法测定左金丸中吴茱萸次碱含量的方法。方法 采用高效液相色谱法。IntersilC18分析色谱柱(4.6mm ID×250mm,粒径5μm),流动相为乙腈—10%乙腈(50:50),流速1ml·min-1,检测波长345nm。结果 吴茱萸次碱的理论板数为2100。回归方程Y=-0.2197+0.0000003484X(r=0.9999),线性范围12～60μm·ml-1。平均回收率为96.9%(n=5),RSD 3.7%,最低检出浓度为0.lμg·ml-1。结论 方法简便,结果准确。
Abstract:
OBJECTIVE A quantitative method was developed for the determination of rutaecarpine in Zuojin Wan by HPLC. METHOD The chromatographic conditions include column Intersil C18, mobile phase:acetonitrile-10% acetonitrile (50:50), flow rate 1 ml·min-1, wavelength 345nm. RESULTS The number of theoretical plates caculated by rutaecarpine peak was 2100, The regression equation of rutaecarpine was Y=-0.2197+0.000000349X(r=0.9999) and the liner range was 12-60μg·ml-1. The average recovery of rutaecarpine was 96.9% (n=5),RSD 3.7%. The measurable lowest limit of rutaecarpine was 0.1μg·ml-. CONCLUSION The method is sensitive, rapid and accurate.
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