摘要:
目的:分析人参茎叶总皂苷中人参皂苷Re的含量,为人参总皂苷的质量控制提供依据。方法:色谱柱:D iamonsil C18(4.6 mm×25 mm,5μm),保护柱:D IKMA EasyGuard C18(10 mm×4.6 mm),流动相:乙腈-0.5%冰醋酸水溶液,梯度洗脱,流速:1.25 mL/m in;ELSD:漂移管温度40℃,载气压力3.5 bar,放大系数为7。结果:标准曲线:C=1.191×10-7A-0.187 6,线性范围:0.038～1.14 mg/mL,r=0.999 3,检出限:20 ng(S/N>3),加样回收率:96.84%～104.21%。结论:该方法前处理简单,分析准确、快速,可作为人参茎叶总皂苷中人参皂苷Re的含量分析方法。
Abstract:
Objective : To determine quantitatively ginsenoside Re in total ginsenoside from stalk and leaf of Panax ginseng,and to provide experimental evident for total ginsenoside quality control of Panax ginseng. Methods :The method was developed with the condition as follows:Diamonsil C18(4.6 mm×25 mm,5 μm) and DIKMA EasyGuard C18(10 mm×4.6 mm) was used as chromatographic column and guard column respectively.Gradient elution was employed with the mobile phase of acetonitrile-0.5% acetic acid/water at a flow rate of 1.25 mL/min.ELSD:temperature of the drift tube 40 ℃;gas pressure: 3.4 bar;gain: 7. Results :The standard curve: C=1.191×10-7A-0.187 6;the linear range: 0.038～1.14 mg/mL,r=0.999 3;the limits of detection: 20 ng(S/N>3);recovery rate of the added sample: 96.84%～104.21%. Conclusion :With its accuracy,sensitivity,reproducibility as well as the simplicity of sample preparation,this method is feasible for quality control of total ginsenoside from stalk and leaf of Panax ginseng.
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