HPLC法测定养精胶囊中淫羊藿苷的含量
Determination of icarrin in Yangjing capsule by HPLC
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摘要: 目的: 建立测定养精胶囊中淫羊藿苷含量的高效液相色谱方法。 方法: 采用Phenomenex C18柱(250 mm×4.6 mm,5μm);流动相:乙腈-水(30:70);流速:0.8ml/min;检测波长:270 nm。 结果: 淫羊藿苷在122.2~611.0 ng范围呈良好线性关系,r=0.999 6,平均回收率98.2%,RSD 1.33%。 结论: 本法简便可行,准确性高,重现性好,适用于该制剂含量控制。Abstract: Objective:To establish an HPLC method for determination of Icarrin in Yangjing Capsule. Methods: The determina- tion was performed on Phenomenex C18 column(250mm×4.6 mm,5μm).The mobile phase consisted of acetonitrile-water(30:70) with a flow rate of 0.8 mL/min.The detecting wavelength was at 270 nm. Results: The linear range of icarrin was 122.2~611.0 ng (r=0.9996).The average recovery was 98.2%(RSD:1.33% ). Conclusion: The established method is simple,convenient,accu- rate and reproducible,and suitable for the quality control of Icarrin content in Yangjing capsule.
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Key words:
- Yangjing capsule /
- icarrin /
- HPLC
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