Determination of sinoporphyrin sodium in tumor-bearing mouse plasma by HPLC method
-
摘要: 目的 建立HPLC法测定荷瘤小鼠体内华卟啉钠的含量，并分析其在荷瘤小鼠体内的药动学情况。 方法 采用Waters XBridge C18（3.0 mm×100 mm，3.5 μm）色谱柱，流动相为A：乙腈-甲醇（20：80），B：水（1%乙酸、0.1%三乙胺），梯度洗脱，流速0.7 ml/min，检测波长380 nm。荷瘤小鼠尾静脉给药后在规定时间点采血，分离得到血浆，乙腈沉淀蛋白后按照上述方法进行样品检测，采用DAS 2.0软件进行统计矩计算和分析。 结果 华卟啉钠浓度在70.8~14 160 ng/ml范围内线性关系良好（r=0.999 8）。华卟啉钠主要药动学参数：cmax=（24 127.59±1 415.23）ng/ml，tmax=0.083 h，t1/2=（9.59±1.25）h，MRT0-∞=（11.77±1.73）h，AUC0-∞=（34 775.83±6 185.43）h·ng/ml。 结论 该方法充分考虑光敏剂华卟啉钠的样品稳定性对检测准确度的影响，具有灵敏、快速、准确、样品处理简单等特点，适用于华卟啉钠临床前荷瘤小鼠血浆样本的分析和研究。Abstract: Objective To establish a HPLC method for the assay of sinoporphyrin sodium (DVDMS) in tumor-bearing mouse plasma and to study its pharmacokinetics. Methods The column was Waters XBridge C18 (3.0 mm×100 mm, 3.5 μm). Gradient elution was applied with mobile phase A as the mixture of acetonitrile-methanol (20:80) and B as the aqueous solution of 1% acetic acid and 0.1% triethylamine at flow rate 0.7 ml/min. The detection wavelength was 380 nm. DVDMS was administrated to tumor-bearing mice by tail vein injection. The blood samples were collected at designated time and centrifuged for plasma. DVDMS in plasma samples were extracted by protein precipitation and analyzed by the HPLC method mentioned above. Pharmacokinetic parameters were calculated by DAS 2.0 with statistical moment analysis. Results DVDMS showed good linearity within the ranges of 70.8-14 160 ng/ml (r=0.9998). The main pharmacokinetic parameters were calculated as follows:cmax=(24 127.59±1 415.23) ng/ml, tmax=0.083 h, t1/2=(9.59±1.25) h, MRT0-∞=(11.77±1.73) h, AUC0-∞=(34 775.83±6 185.43) h·ng/ml. Conclusion This HPLC method is sensitive, rapid and accurate, which can be used for analysis and research of DVDMS in plasma samples of tumor-bearing mice.
-
Key words:
- sinoporphyrin sodium /
- HPLC /
- pharmacokinetics
-
[1] 方起程,杨栋.以醚键结合的卟啉二聚体盐及其制造方法:102030765 B[P]. 2011-04-27. [2] Liu Y, Wang P, Liu Q, et al. Sinoporphyrin sodium triggered sono-photodynamic effects on breast cancer both in vitro and in vivo[J]. Ultrason Sonochem, 2016, 31:437-448. [3] Wang X, Hu J, Wang P, et al. Analysis of the In Vivo and In Vitro effects of photodynamic therapy on breast cancer by using a sensitizer, sinoporphyrin sodium[J]. Theranostics, 2015, 5(7):772-786. [4] Hu J, Wang X, Liu Q, et al. Antitumor effect of sinoporphyrin sodium-mediated photodynamic therapy on human esophageal cancer Eca-109 cells[J].Photochem Photobiol, 2014, 90(6):1404-1412. [5] Shi R, Li C, Jiang Z, et al. Preclinical study of antineoplastic sinoporphyrin sodium-PDT via In Vitro and In Vivo models[J]. Molecules, 2017, 22(1), 112. [6] Lin N,Li C,Wang, et al. A safety study of a novel photosensitizer, sinoporphyrin sodium, for photodynamic therapy in Beagle dogs[J].]. Photochem Photobiol Sci, 2015, 14(4):815-832. [7] 吕海燕. 新型光敏剂以醚键结合的卟啉二聚体盐(DVDMS-2)的初步临床前研究[D]. 厦门:厦门大学, 2012. [8] 杨翠平,李烨,王中华,等. 华卟啉钠在SD大鼠体内代谢产物的质谱分析[J]. 解放军药学学报, 2016,32(2):106-110. [9] 杨翠平,李烨,王爱平. 华卟啉钠在不同种属肝微粒体中的代谢研究[C]. 2015年(第五届)药物毒理学年会论文集, 2015. [10] Cramers P, Ruevekamp M, Oppelaar H, et al. Foscan uptake and tissue distribution in relation to photodynamic efficacy[J]. Br J Cancer, 2003, 88(2):283-290. [11] Peng Q, Moan J, Kongshaug M, et al. Sensitizer for photodynamic therapy of cancer:a comparison of the tissue distribution of Photofrin Ⅱ and aluminum phthalocyanine tetrasulfonate in nude mice bearing a human malignant tumor[J]. Int J Cancer, 1991, 48(2):258-264. 期刊类型引用(9)
1. 强卫平，官蜀钧. 浅析“保胃气”理论在防治脑出血并发症中的临床意义. 陕西中医药大学学报. 2022(02): 53-56 . 
百度学术2. 刘洪涛，冯守宁，董梁. 雷尼替丁联合奥美拉唑治疗重度颅脑损伤并发上消化道出血患者的效果观察. 华夏医学. 2022(02): 1-5 . 百度学术3. 王琤毓，陈明红. 基于药物经济学研究的方法对质子泵抑制剂预防重型颅脑损伤患者应激性溃疡的分析. 海峡药学. 2020(09): 151-153 . 百度学术4. 高慧，杨洁，胡云霞，陈秋华，鲁俊，王醒，陈明祺. 升降散对重型颅脑损伤患者胃肠功能障碍及预后影响的临床研究. 中国中医急症. 2019(04): 565-567+571 . 百度学术5. 白荷荷，聂晓静，王园姬. 2014—2016年西安市中心医院质子泵抑制剂的使用情况分析. 现代药物与临床. 2018(07): 1814-1819 . 百度学术6. 陈向荣，杜菊梅，张磊，吴宗涛，徐东泽. 三七白及散对基底节区、丘脑出血患者胃黏膜灌注及应激性溃疡发生率的影响. 中医药导报. 2018(18): 96-98 . 百度学术7. 李鹏，刘建萍，黄雪英. 奥美拉唑不同给药方案预防重型颅脑损伤应激性溃疡的疗效评价. 江西医药. 2016(08): 792-793 . 百度学术8. 巩莉. 质子泵抑制剂诱发感染危险性研究进展. 山西医药杂志. 2016(14): 1659-1660 . 百度学术9. 孙会芳. 重症监护病房重型颅脑损伤患者继发肺部感染的相关因素分析. 中国药物经济学. 2016(06): 127-129 . 百度学术其他类型引用(3)
-
点击查看大图
计量
- 文章访问数: 4237
- HTML全文浏览量: 554
- PDF下载量: 678
- 被引次数: 12
下载:
