摘要:
目的 确定白及的止血活性部位,为其止血活性成分的筛选提供线索和依据。 方法 用70%乙醇(EtOH)对白及原药材进行渗漉提取,然后,依次用石油醚(Pet)、乙酸乙酯(EtOAc)、正丁醇(n-BuOH)对醇浸膏进行萃取,分别制得Pet部分、EtOAc部分、n-BuOH部分和H2O部分,通过测定小鼠凝血时间和出血时间,考察其活性部位。 结果 n-BuOH部分和H2O部分具有止血作用,而EtOAc部分具有延长凝血、出血时间作用。 结论 白及中具有止血作用的活性成分主要存在于n-BuOH部分和H2O部分,而EtOAc部分具有活血作用。
关键词:
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白及 /
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出血 /
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止血 /
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小鼠 /
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活性
Abstract:
Objective To determine the active position of Bletilla colloid and offer references to the seleciton of its active composition. Methonds The crude Bletilla colloid was extracted by 70% ethanol permeating , then the petroleum ether extract,ethyl acetate exctract ,n-butyl alcohol extract and water extract were exctracted from alcohol extract in turn by petroleum,ethyl acetate,n-butyl alcohol extraction. The active position was determined by measuring the time of mice blood coagulation and hemorrhage. Results N-butanol extract and water extract from Bletilla colloid had hemostasis effect,ethyl acetate extract had the effect of prolonging the time of blood coagulation and hemorrhage. Conclusion N-butanol extract and water extract from Bletilla colloid was major active extracts played a part in hemostasis, ethyl acetate extract had the effect of invigoration the circulation of blood.
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