心肌磷酸腺苷的高效液相色谱测定条件的优化
Optimization of HPLC conditions in determining the adenosine phosphate in cardiac muscle
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摘要: 目的 优化心肌样品中的磷酸腺苷的高效液相色谱法测定条件。方法 固定其他色谱条件,改变流动相组成与配比,以分离结果的优劣优化流动相。结果 甲醇-水(2:98)(含0.15mol/L磷酸二氢钠,pH=6.0)为局部最佳流动相条件。结论 本条件可以实现该样品的准确、快速、经济实用的分析。Abstract: OBJECTIVE To optimize the chromatographic conditions in determining the adenosine phosphate in cardiac muscle samples.METHODS Fixing other conditions than the composition and ratio of the mobile phase,estimate the optimum according to the separation results.RESULTS The local optimal mobile phase is composed of methanol,distilled water(2:98) and 0.15mol/L sodium dihydrophophate.CONCLUSIONS The conditions help to an accurate,fast and inexpensive analysis of the adenosine phosphates.
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