Research on the determination of total flavonoids in compound Yinchen mixture
-
摘要: 目的 建立紫外-可见分光光度法测定复方茵陈合剂中总黄酮含量。 方法 以芦丁作为对照,依次用直接测定法、氯化铝-醋酸钠显色法、硝酸铝显色法,探究最优测定方法。 结果 本品最优显色法为硝酸铝显色法,测定波长为508 nm,在0.012 5~0.062 6 g/L范围内线性关系良好,线性回归方程为Y=12.127 30 X+0.000 14(r=0.999 9),加样回收率平均值为99.49%(n=9,RSD为0.84%)。 结论 硝酸铝显色法可作为复方茵陈合剂中总黄酮的含量测定方法,该法简便、快速、稳定可靠。Abstract: Objective To establish a method for the determination of the total flavonoids content in compound Yinchen mixture by UV spectrophotometry. Methods Using rutin as comparison, three coloration methods were studied to find the optimal assay method. Results The sample was detected at 508 nm wavelength by NaNO2-Al(NO3)3-NaOH reaction with rutin as reference. The rutin content had a liner relationship in the range of 0.012 5-0.062 6 g/L (n=9,r=0.999 9), and the average recovery rate was 99.49% with RSD of 0.84%. Conclusion The NaNO2-Al(NO3)3-NaOH coloration method is proved to be simple, quick, stable and reliable for the determination of total flavonoids in compound Yinchen mixture.
-
[1] 牛筛龙, 张兴耐, 吴之琳, 等. 茵陈总黄酮对急性肝损伤大鼠的保护作用[J]. 医药导报, 2016, 35(3):246-248. [2] 张玲, 李俊, 黄艳, 等. 野菊花总黄酮对酒精致急性肝损伤小鼠的保护作用[J]. 安徽医药, 2011, 15(10):1197-1200. [3] 曹锦花. 茵陈的化学成分和药理作用研究进展[J]. 沈阳药科大学学报, 2013, 30(6):489-494. [4] 王丽红, 宋洋, 肖艳, 等. 茵陈化学成分的分离与鉴定[J]. 中国药房, 2011, 22(11):1020-1022. [5] 章林平,孙倩,王威,等.茵陈有效成分的药理作用及其临床应用的研究进展[J].抗感染药学,2014,11(1):28-31. [6] 曾棋平,曹毅祥,黄丽珊,等.复方首乌藤合剂的薄层鉴别和总黄酮的含量测定[J].药学实践杂志,2016,34(5):424-427. [7] 曾令军,柯小温,宋洪涛.白子草总黄酮及总多糖降血糖有效部位的提取工艺研究[J].药学实践杂志,2016,34(6):516-521. [8] 邢桂珍,陶卿,崔玉梅,等. UV法和HPLC法测定甘草总黄酮混悬液中总黄酮和查尔酮含量[J].安徽医药,2014,18(2):2251-2255. [9] Chen ML, Hu W, Zhang C, et al. Capillary electrophoresis method for the determination of flavonoids[J].Yao Xue Xue Bao, 2011, 46(8):883-889. [10] 韦瑀龙, 黄小鸥, 蓝晓庆. 固本补肾口服液的总黄酮含量测定方法研究[J]. 实用药物与临床, 2016, 19(10):1287-1289. [11] 匡海学. 中药化学[M].2版.北京:中国中医药出版社,2003:138-139. [12] 任珊珊,包保全,毛婷,等.中药中总黄酮的含量测定方法研究进展[J].北方药学,2015,12(3):112-115. [13] 马雯芳,邓慧连,蔡毅,等.冰糖草总黄酮含量测定及显色方法优化[J].中国实验方剂学杂志,2013,19(3):112-115. [14] 郑媛媛,李辰,封士兰,等.油橄榄叶中总黄酮含量测定方法探讨[J].光谱学与光谱分析,2011,31(2):547-550.
计量
- 文章访问数: 2707
- HTML全文浏览量: 278
- PDF下载量: 588
- 被引次数: 0