Determination of matrine in Lefu oral liquid by HPLC
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摘要: 目的 建立HPLC法测定乐肤口服液中苦参碱含量的方法。 方法 色谱柱为Diamonsil Platisil NH2柱（4.6 mm×250 mm， 5 μm），流动相为乙腈-异丙醇-3%磷酸水溶液（84：4：12）；流速为1.2 ml/min；进样量5 μl；检测波长205 nm。 结果 苦参碱在54.50~872.00 μg/ml浓度范围内线性关系良好，r=0.999 1；平均回收率为99.82%， RSD=1.12%。 结论 该法简便、稳定可靠、重复性好，为控制乐肤口服液的质量标准提供了可借鉴的依据。Abstract: Objective To establish a high performance liquid chromatography method for the determination of matrine in Lefu oral liquid. Methods The HPLC method was performed on a Diamonsil Platisil NH2 column (4.6 mm×250 mm, 5 μm) with the mobile phase of acetonitrile-isopropyl alcohol-3% phosphoric acid solution (84:4:12). The flow rate was 1.2 ml/min. The sample injection volume was 5 μl. The detective wavelength was 205 nm. Results The calibration curve of matrine showed good linear response ranged from 54.50 to 872.00 μg/ml with r=0.999 1. The average recovery of spiked samples for matrine was 99.82% while the relative standard deviation for repetitions was 1.12%. Conclusion The method was simple, reliable and repeatable, which could be used for the quantitative determination of matrine of Lefu oral liquid.
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Key words:
- HPLC /
- matrine /
- Lefu oral liquid
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