Study on quality standards of Hypericum ascyron
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摘要: 目的 完善红旱莲药材的质量标准。 方法 采用显微鉴别法、薄层色谱(TLC)法鉴别红旱莲;按《中华人民共和国药典》(2015年版)规定方法考察水分、总灰分、酸不溶性灰分和醇溶性浸出物;用HPLC法测定金丝桃苷的含量。 结果 建立了红旱莲显微鉴别法和TLC法鉴别,HPLC法含量测定;确定了水分、灰分、醇溶性浸出物的限度。 结论 本实验为红旱莲药材的质量标准提高提供了依据。Abstract: Objective To improve the quality standards of Hypericum ascyron. Methods Hypericum ascyron was identified by microscopic observation and TLC method.The moisture, total ash,acid-insoluble ash and alcohol soluble extract were assayed following the procedures stated in Chinese Pharmacopoeia.The contents were determined by HPLC. Results Identification methods for Hypericum ascyron with microscopy,TLC and HPLC were established.The limits of moisture,total ash and alcohol soluble extract were defined. Conclusion This study provides scientific data for the quality control of Hypericum ascyron.
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Key words:
- Hypericum ascyron /
- microscopic identification /
- TLC /
- HPLC /
- hyperin /
- quality standards
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