摘要:
目的: 建立宣肺清热合剂（麻黄、苦杏仁、浙贝母、黄芩等）中有效成分盐酸麻黄碱和盐酸伪麻黄碱的含量测定方法。 方法: 采用高效液相色谱法,色谱柱Agilent ZORBA×SB-C18（5μm,4.6 mmx 250 mm）,流动相为乙腈-0.02 mol/L磷酸二氢钾（用磷酸调pH至2.7）,（4:100,含0.1%三乙胺）,流速:1.2 ml/min,检测波长:207 nm。 结果: 盐酸麻黄碱在11.04-222.8μg/mL（r=0.999 9）,盐酸伪麻黄碱在8.27-165.4μg/ml（r=0.999 9）的浓度范围内均线性关系良好,回收率均在95%-105%之间,RSD均小于3%。 结论: 本方法操作简便、重现性好,可以更有效地控制制剂的质量。
Abstract:
Objective To set up a method of simultaneously determining the contents of ephedrine and pseudoephedrine in Xuanfei Qingre mixture(Herba Ephedrae,Semen Armeniacae amarum,Bulbus Fritillaria thunbergii,Radix scutellaria,etc). Methods The HPLC-quantitative analysis was carried out on a column of Agilent ZORBA× SB-CC18 by using a mobile phase of acetonitrile-0.02 mol/L potassium dihydrogen phosphate(adjusted with phosphoric acid pH=2.7),(4100,with 0.1% triethylamine)under a flow rate of 1.2 ml/min.207 nm was selected as the wavelength of detector. Results The linear range of ephedrine was 11.04～222.8 μg/ml(r=0.999 9)and pseudoephedrine's was 8.27～165.4 μg/ml(r=0.999 9).The recoveries of them were all between 95% and 105%(RSD<3%). Conclusion This method is simple,reproducible and specific.
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