HPLC-ELSD法测定瓜子金中瓜子金皂苷己的含量
Determination of polygalasaponin F in Polygala japonica by HPLC-ELSD method
-
摘要: 目的: 建立测定瓜子金皂苷己的含量的法,为瓜子金药材质量控制提供依据。 方法: 采用HPLC-ELSD法;色谱柱:SHISEIDO Capcell Pak C18柱;流动相:乙腈-水(30:70),等度洗脱;流速:1.0 ml/min;柱温:25℃,检测器:蒸发光散射检测器。 结果: 瓜子金中瓜子金皂苷己获得良好分离,在0.346-3.460μg范围内呈现良好线性关系;精密度试验RSD为1.64%;重现性试验RSD为2.12%;加样回收率为99.85%。 结论: 建立了HPLC-ELSD法测定瓜子金皂苷己的含量,方法简单可行。Abstract: Objective To establish an effective quality control method of the traditional Chinese medicine——Polygala japonica Houtt. Methods HPLC-ELSD method was adopted for the determination of polygalasaponin F in Polygala japonica Houtt.The analysis was performed on a SHISEIDO Capcell Pak C18(4.6 mm×250 mm,5 μm) as the analytical column,acetonitrile-water(3070,v/v) as the mobile phase.The flow rate was 1.0 ml/min.The column temperature was at 25 ℃. Results The validation was carried out and the linearity(r2 = 0.999 8) between 0.346 μg and 3.460 μg and recovery(99.85%) were acceptable.The precision and reproducibility were evaluated with RSD values 1.64% and 2.12%,respectively. Conclusion This method is simple and feasible for the determination of polygalasaponin F in Polygala japonica Houtt.
-
Key words:
- Polygalasaponin F /
- HPLC /
- ELSD
计量
- 文章访问数: 2240
- HTML全文浏览量: 162
- PDF下载量: 734
- 被引次数: 0