细菌广谱酶与超广谱酶对头孢噻肟的水解作用及酶抑制剂抑酶效应

张翔; 雷军; 袁斌; 刘刚; 赵廷坤; 凌保东

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

x 关闭永久关闭

重要提示

《药学实践与服务》杂志不接收生物战剂、毒剂相关内容的稿件。对于涉军信息类论文如需投稿，请务必投稿前电话或邮箱咨询。联系电话：021-81871323，邮箱：yxsjzzs@163.com。敬请谅解，谢谢配合！

《药学实践与服务》编辑部

重要通知

《药学实践与服务》杂志目前不收取审稿费、版面费、加急费等费用，如收到邮件声称是编辑部X编辑，要求加作者微信的，或填写编委登记申请表的，请谨防诈骗！

编辑部用于作者校稿时微信绑定的邮件是通过官方邮箱：yxsjzzs@163.com发送的，标题是《药学实践与服务》XML数字出版服务微信绑定，请区分开！如有疑虑，请联系编辑部，电话：021-81871323。

《药学实践与服务》编辑部

细菌广谱酶与超广谱酶对头孢噻肟的水解作用及酶抑制剂抑酶效应

张翔, 雷军, 袁斌, 刘刚, 赵廷坤, 凌保东

文章导航 > 药学实践与服务 > 2007 > (6): 376-378

张翔, 雷军, 袁斌, 刘刚, 赵廷坤, 凌保东. 细菌广谱酶与超广谱酶对头孢噻肟的水解作用及酶抑制剂抑酶效应[J]. 药学实践与服务, 2007, (6): 376-378.

引用本文:

张翔, 雷军, 袁斌, 刘刚, 赵廷坤, 凌保东. 细菌广谱酶与超广谱酶对头孢噻肟的水解作用及酶抑制剂抑酶效应[J]. 药学实践与服务, 2007, (6): 376-378.

ZHANG Xiang, LEI Jun, YUAN Bin, LIU Gang, ZHAO Ting-kun, LING Bao-dong. Studies on hydrolysis of cefotaxime by broad and extended-spectrum-β-lactamase and inhibitory actions of tazobactam and sulbactam[J]. Journal of Pharmaceutical Practice and Service, 2007, (6): 376-378.

Citation:

ZHANG Xiang, LEI Jun, YUAN Bin, LIU Gang, ZHAO Ting-kun, LING Bao-dong. Studies on hydrolysis of cefotaxime by broad and extended-spectrum-β-lactamase and inhibitory actions of tazobactam and sulbactam[J]. Journal of Pharmaceutical Practice and Service, 2007, (6): 376-378.

细菌广谱酶与超广谱酶对头孢噻肟的水解作用及酶抑制剂抑酶效应

川北医学院药物研究所,四川南充637007

基金项目: 四川省教育厅自然科学重点科研项目(No.2005A081)

Studies on hydrolysis of cefotaxime by broad and extended-spectrum-β-lactamase and inhibitory actions of tazobactam and sulbactam

Institute of Pharmacology,North Sichuan Medical College,Nanchong 637007,China

摘要: 目的:观察比较头孢噻肟(CTX)对细菌广谱及超广谱β-内酰胺酶的稳定性以及三唑巴坦(TAZ)和舒巴坦(SBT)的抑酶效应。方法:用超声破碎法提取60株本院附属医院临床分离菌的β-内酰胺酶,用三维试验法检测β-内酰胺酶酶型;用紫外分光光度计检测酶活性及酶对CTX的水解率。结果:60株细菌中产超广谱酶者31株,产广谱酶者29株;两类酶的活性为非正态分布,其中位数分别为3 094 U和528 U(P<0.05)。3种常见菌的酶活性高低依次为大肠埃希菌、阴沟肠杆菌和铜绿假单孢菌(中位数分别为2656 U,1185 U和165.5 U,P<0.05)。超广谱酶对CTX的水解率(中位数20.10%)显著高于广谱酶(中位数3.15%)(P<0.05)。TAZ和SBT均能显著抑制两类酶对CTX的水解作用,但两种酶抑制剂的作用无差异(P>0.05)。结论:超广谱酶(ESBLs)对CTX的水解作用较广谱酶强,TAZ和SBT能有效抑制两类酶对CTX的水解,但其抑制作用强度基本相同。
- 头孢噻肟 /
- 三唑巴坦 /
- 舒巴坦 /
- 超广谱β-内酰胺酶 /
- 广谱酶 /
- 革兰阴性杆菌 /
- 水解
Abstract: Objective :To observe the stability of cefotaxime(CTX) to broad-and extended-spectrum β-lactamase,and to compare inhibiting-βlactamase activities of tazobactam and sulbactam. Methods : 60 strains of Gram-negative bacilli from Affiliated Hospital of North Sichuan Medical College were dealed with ultrasonic wave and their β-lactamases were extracted and these enzymes were detected by three-dimensional test.β-lactamase activitiy and hydrolysis of CTX were quantitated spectrophotometrically and calculated. Results :In these β-lactamases of 60 strains of Gram-negitive bacilli,the number of extended-spectrum β-lactamases and broad-spectrum β-lactamases were 31 and 29,respectively,and their medians of enzyme activities were 3 094 U and 528 U(P<0.05).The medians of β-lactamases activities of Escherichia coli,Enterobacter cloacae and Pseudomonas aeruginosa were 2 656 U,1 185 U and 165.5 U,respectively.The median of hydrolysis rates(HR) of CTX by ESBLs and BSBLs were 20.10% and 3.15%,respectively(P<0.05).The combination of CTX/TAZ and CTX/SBT(the ratio=2-1) could make the HR drop significantly(P<0.05),but the effects of TAZ and SBT were of no differences(P>0.05). Conclusion :The hydrolysis activities of ESBLs on CTX was higher than that of BSBLs.TAZ and SBT could effectively inhibit hydrolysis of CTX by β-lactamases,but these two inhibitors had little difference in their potency.
- cefotaxime /
- tazobactam /
- sulbactam /
- extended-spectrum β-lactamase /
- broad-spectrum β-lactamase /
- Gram-negative bacilli /
- hydrolysis

[1]	周文铂, 李伟林, 戴吴婷, 刘瑞瑶, 俞媛. 载葡萄糖氧化酶纳米凝胶的构建及对胶质瘤细胞Warburg效应的抑制 . 药学实践与服务, 2026, 44(): 1-6. doi: 10.12206/j.issn.2097-2024.202511030
[2]	孟盼盼, 宋慕格, 杨世超, 陈克明, 杨中铎, 马慧萍. 基于Keap1/Nrf2/HO-1信号通路研究利舒康胶囊对高原缺氧小鼠学习记忆障碍的改善作用 . 药学实践与服务, 2026, 44(1): 26-31, 52. doi: 10.12206/j.issn.2097-2024.202303006
[3]	倪晓霞, 陈巧秀, 杨育儒, 曹毅祥. 基于谱效关系的黄连不同方法提取物抑菌作用物质基础初步研究 . 药学实践与服务, 2026, 44(2): 80-84. doi: 10.12206/j.issn.2097-2024.202403054
[4]	魏莱, 董国强. SARS-CoV-2主蛋白酶抑制剂和降解剂研究进展 . 药学实践与服务, 2025, 43(6): 259-269, 297. doi: 10.12206/j.issn.2097-2024.202503064
[5]	魏莱, 董国强, 盛春泉. 靶向SARS-CoV-2主蛋白酶的PROTAC设计、合成与蛋白降解活性研究 . 药学实践与服务, 2025, 43(5): 235-241, 258. doi: 10.12206/j.issn.2097-2024.202503063
[6]	赵凤平, 于镇铭, 陈熹, 姜棋文, 郑灿辉, 陈新. 醛脱氢酶2改善铁死亡相关疾病预后的研究进展 . 药学实践与服务, 2025, 43(): 1-7. doi: 10.12206/j.issn.2097-2024.202411043
[7]	赵雪, 顾妍秋, 楚皓文, 吴彩胜, 李镐, 陈啸飞. 基于超高效液相色谱-四极杆飞行时间质谱的麻杏石甘汤化学成分分析 . 药学实践与服务, 2025, 43(11): 548-554, 571. doi: 10.12206/j.issn.2097-2024.202306028
[8]	吴海韵, 杨甜, 张弛, 梁文仪, 苏娟. 气相色谱-离子迁移谱技术在中药研究中的应用进展 . 药学实践与服务, 2025, 43(): 1-6. doi: 10.12206/j.issn.2097-2024.202409007
[9]	邵尉, 袁妮, 刘叶, 于飞, 柳莹, 王峰. 丙戊酸钠与左乙拉西坦治疗儿童癫痫病的成本-效果分析 . 药学实践与服务, 2025, 43(8): 1-5. doi: 10.12206/j.issn.2097-2024.202408046
[10]	詹济华, 颜滢. 低蛋白血症对头孢哌酮舒巴坦血药浓度的影响 . 药学实践与服务, 2025, 43(7): 344-347. doi: 10.12206/j.issn.2097-2024.202309040
[11]	葛鹏程, 苏日古嘎, 任天舒, 党大胜. 硫酸黏菌素联合头孢哌酮舒巴坦治疗泛耐药鲍曼不动杆菌肺内感染的疗效分析 . 药学实践与服务, 2025, 43(): 1-4. doi: 10.12206/j.issn.2097-2024.202404093
[12]	徐飞, 刘盈, 殷佳, 诸国樑, 练鲁英. 上海某三级公立医院药品管理内部控制评价实践研究 . 药学实践与服务, 2024, 42(12): 542-548. doi: 10.12206/j.issn.2097-2024.202402003
[13]	石晓萍, 吕迁洲, 李晓宇, 许青. 泊沙康唑对比伏立康唑经验治疗或诊断驱动治疗免疫功能低下患者侵袭性霉菌病的成本-效果分析 . 药学实践与服务, 2024, 42(12): 512-519. doi: 10.12206/j.issn.2097-2024.202401050
[14]	白学鑫, 陈玉平, 盛春泉, 武善超. 具核梭杆菌小分子抑制剂的筛选及其抗结直肠癌活性研究 . 药学实践与服务, 2024, 42(12): 503-507. doi: 10.12206/j.issn.2097-2024.202405009
[15]	杨凤艳, 张月, 陈恩贤, 缪雪蓉, 魏凯. 瑞马唑仑临床应用研究进展 . 药学实践与服务, 2024, 42(9): 365-374. doi: 10.12206/j.issn.2097-2024.202405026
[16]	钱淑雨, 李铁军. 耐碳青霉烯类肠杆菌耐药机制的研究进展 . 药学实践与服务, 2024, 42(10): 419-425. doi: 10.12206/j.issn.2097-2024.202405005
[17]	冯志惠, 邓仪卿, 叶冰, 安培, 张宏, 张海军. 雀梅藤石油醚提取物诱导三阴性乳腺癌细胞凋亡的实验研究 . 药学实践与服务, 2024, 42(6): 253-259. doi: 10.12206/j.issn.2097-2024.202311055
[18]	夏哲炜, 曾垣烨, 朱海菲, 李育, 陈啸飞. 核磁共振磷谱法测定磷酸氢钙咀嚼片中药物含量 . 药学实践与服务, 2024, 42(9): 399-401, 406. doi: 10.12206/j.issn.2097-2024.202404063
[19]	赖立勇, 夏天爽, 徐圣焱, 蒋益萍, 岳小强, 辛海量. 中药青蒿抗氧化活性的谱效关系研究 . 药学实践与服务, 2024, 42(5): 203-210, 216. doi: 10.12206/j.issn.2097-2024.202211012
[20]	戴菲菲, 傅翔, 陈琼年, 俞苏纯. 上海某二级医院革兰阴性菌流行特征的回顾性分析 . 药学实践与服务, 2024, 42(12): 528-532. doi: 10.12206/j.issn.2097-2024.202305005

点击查看大图

计量

文章访问数: 3164
HTML全文浏览量: 202
PDF下载量: 32
被引次数: 0

细菌广谱酶与超广谱酶对头孢噻肟的水解作用及酶抑制剂抑酶效应

川北医学院药物研究所,四川南充637007

基金项目: 四川省教育厅自然科学重点科研项目(No.2005A081)

收稿日期: 2006-09-11

关键词:

舒巴坦 /

广谱酶 /

摘要: 目的:观察比较头孢噻肟(CTX)对细菌广谱及超广谱β-内酰胺酶的稳定性以及三唑巴坦(TAZ)和舒巴坦(SBT)的抑酶效应。方法:用超声破碎法提取60株本院附属医院临床分离菌的β-内酰胺酶,用三维试验法检测β-内酰胺酶酶型;用紫外分光光度计检测酶活性及酶对CTX的水解率。结果:60株细菌中产超广谱酶者31株,产广谱酶者29株;两类酶的活性为非正态分布,其中位数分别为3 094 U和528 U(P<0.05)。3种常见菌的酶活性高低依次为大肠埃希菌、阴沟肠杆菌和铜绿假单孢菌(中位数分别为2656 U,1185 U和165.5 U,P<0.05)。超广谱酶对CTX的水解率(中位数20.10%)显著高于广谱酶(中位数3.15%)(P<0.05)。TAZ和SBT均能显著抑制两类酶对CTX的水解作用,但两种酶抑制剂的作用无差异(P>0.05)。结论:超广谱酶(ESBLs)对CTX的水解作用较广谱酶强,TAZ和SBT能有效抑制两类酶对CTX的水解,但其抑制作用强度基本相同。

English Abstract

Studies on hydrolysis of cefotaxime by broad and extended-spectrum-β-lactamase and inhibitory actions of tazobactam and sulbactam

Institute of Pharmacology,North Sichuan Medical College,Nanchong 637007,China

Received Date: 2006-09-11

Keywords:

Abstract: Objective :To observe the stability of cefotaxime(CTX) to broad-and extended-spectrum β-lactamase,and to compare inhibiting-βlactamase activities of tazobactam and sulbactam. Methods : 60 strains of Gram-negative bacilli from Affiliated Hospital of North Sichuan Medical College were dealed with ultrasonic wave and their β-lactamases were extracted and these enzymes were detected by three-dimensional test.β-lactamase activitiy and hydrolysis of CTX were quantitated spectrophotometrically and calculated. Results :In these β-lactamases of 60 strains of Gram-negitive bacilli,the number of extended-spectrum β-lactamases and broad-spectrum β-lactamases were 31 and 29,respectively,and their medians of enzyme activities were 3 094 U and 528 U(P<0.05).The medians of β-lactamases activities of Escherichia coli,Enterobacter cloacae and Pseudomonas aeruginosa were 2 656 U,1 185 U and 165.5 U,respectively.The median of hydrolysis rates(HR) of CTX by ESBLs and BSBLs were 20.10% and 3.15%,respectively(P<0.05).The combination of CTX/TAZ and CTX/SBT(the ratio=2-1) could make the HR drop significantly(P<0.05),but the effects of TAZ and SBT were of no differences(P>0.05). Conclusion :The hydrolysis activities of ESBLs on CTX was higher than that of BSBLs.TAZ and SBT could effectively inhibit hydrolysis of CTX by β-lactamases,but these two inhibitors had little difference in their potency.

张翔, 雷军, 袁斌, 刘刚, 赵廷坤, 凌保东. 细菌广谱酶与超广谱酶对头孢噻肟的水解作用及酶抑制剂抑酶效应[J]. 药学实践与服务, 2007, (6): 376-378.

引用本文:

张翔, 雷军, 袁斌, 刘刚, 赵廷坤, 凌保东. 细菌广谱酶与超广谱酶对头孢噻肟的水解作用及酶抑制剂抑酶效应[J]. 药学实践与服务, 2007, (6): 376-378.

Citation:

ZHANG Xiang, LEI Jun, YUAN Bin, LIU Gang, ZHAO Ting-kun, LING Bao-dong. Studies on hydrolysis of cefotaxime by broad and extended-spectrum-β-lactamase and inhibitory actions of tazobactam and sulbactam[J]. Journal of Pharmaceutical Practice and Service, 2007, (6): 376-378.