紫外分光光度法测定心脏停搏液中盐酸普鲁卡因的含量
Determination of procaine hydrochloride in cardiac standstill solution by ultraviolet spectrophotometry
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摘要: 目的:建立心脏停搏液中盐酸普鲁卡因的含量测定方法。方法:采用紫外分光光度法,检测波长为290 nm。结果:盐酸普鲁卡因在0.5-8μg/mL范围内与吸收度呈线性关系(r=0.9999),平均回收率为98.59%,RSD为0.033%,n=9。结论:该方法操作简便、快速,结果准确,专属性强,适用于心脏停搏液中普鲁卡因的含量测定。Abstract: Objective :To establish a method for the determination of the content of procaine hydrochloride in cradiac standstill solution. Method:Ultraviolet spectrophotometric method was used to directly determine the content of procaine hydrochloride.The wavelength of detection was 290nm. Results :The liner range was 0.5～8μg/mL(r=0.999 9).The relative recovery of the method was 98.59% with RSD of 0.033%. Conclusions : This method is simple,rapid,accurate and can be used to determine the content of procaine hydrochloride in the preparation.
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