高效液相色谱-蒸发光散射法测定白消安片的含量
Quantitative determination of content in Busulfan's tablets by HPLC-ELSD
-
摘要: 目的 :建立HPLC-ELSD法考察并测定白消安片中白消安的含量。 方法 :SHIMADZU C8柱(250mm×4.6min,5μm),流动相:水:乙腈(75:25),流速:1.0mL/min,载气:氮气,载气流速:2.0bar。 结果 :白消安在浓度0.25-0.75mg/mL范围内与峰面积的对数呈良好的线性关系,r为0.9999。平均回收率为99.7%。 结论 :本方法专属性好,准确,可用于该制剂中白消安含量的测定。Abstract: Objective :To establish a HPLC-ELSD method for determining the content of busulfan in busulfan tablets. Methods : The chromatographic conditions were as follows:SHIMADZU C8 column(250mm×4.6mm,5μm),the mobile phase:water-acetonitrile (75-25),the flow rate:1.0mL/min,and the detector:ELSD,the carrier gas:nitrogen,the flow rate:2.0 bar. Results :The standard curve of busulfan was linear over the rage of 0. 25-0.75mg/mL(r=0.9999).The average recovery was 99.7%. Conclusion :This method is accurate and specificity,and it can be used on the quality control of busulfan tablets.
-
Key words:
- HPLC-ELSD /
- busulfan /
- determination of content
计量
- 文章访问数: 2063
- HTML全文浏览量: 183
- PDF下载量: 187
- 被引次数: 0