复方大青叶合剂中绿原酸TLC方法改进
The improvement of TLC identification method of chlorogenic acid in Fufang Daqingye Heji
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摘要: 目的 对复方大青叶合剂中绿原酸的鉴别方法进行改进。方法 将展开剂中各组分的比例,醋酸乙酯-甲醇-水(10:2:3)改为醋酸乙酯-甲醇-水(10:4:6),结果判断由先在紫外灯(365nm)下观察荧光后,再置氨气中熏,显相同的黄色斑点,改为先置氨气中熏后再在紫外灯(365nm)下观察荧光。在室温10℃-30℃,相对湿度30%-70%进行考察。结果 薄层色谱斑点清晰,易于判断。Abstract: OBJECTIVE To improve the judgement method of Chloragenic acid in Fufang Daqingye Heji. METHODS To change the eluant of ethyl acetate - methanol - water form(10:2:3)to(10:4:6)and change the turns by smoked the thin layer in ammonia first and then observe the fluresence under the ultraviolet rays lamp(365 nm)instead of the common turns of observation first,smoke second. They show the Same yellow spot, under the condition of 10 to 30 degrees centigrade and relative humidity 30 to 70 percent. RESULTS:The TLC spots is clear and is easy to check out.
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Key words:
- Fufang Daqingye Heji /
- chloragenic acid /
- eluant /
- RF valoue
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