HPLC法测定人血清中氨氯地平浓度
Determination of amlodipine in serum by HPLC
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摘要: 本文建立了快速、简便测定人血清中氨氯地平的HPLC法,分析柱为 ODS-5S(150×4.0mm)不锈钢柱,流动相为甲醇:0.03M磷酸二氢钾缓冲液(9:1)的混合液.流速为1.0ml/min,以硝苯地平为内标物,检测波长为366nm,蛋白沉淀剂选用15%高氨酸.氨氯地平测定的线性范围为4～12μg/ml,r=0.9992,最低检测限度为 4ng.日内平均相对回收率为10.24±5.40%,日间平均相对回收率为99.87±6.23%.本方法专一性好,放置24h对含量测定无影响.Abstract: A rapid specific and sensitive HPLC menthod for the determination of amlodipine in serum was developed, using a stainless steel column (ods-5s, 150×4.0mm) with nifedipine as internal standard. The mobile phase was a mixture of methanol-KH2PO4 buffer(0.03mol/L), flow rate 1ml/min. Wavelength of the detector was set at 300nm. The linear correlattion was observed within the range of 4-12μg/ml(r=0.9992) and detect limitation at 4ng. The mean recoveries of the method were 100.24±5.4O% (intra-day)and 99.87±6.23% (inter-day).
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Key words:
- amlodipine /
- HPLC /
- the determination of conten
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