复元醒脑汤对大鼠局灶性脑缺血再灌注损伤的保护作用研究

李莉; 冯晶晶; 李铁军; 章越凡

doi:10.3969/j.issn.1006-0111.2018.01.007

复元醒脑汤对大鼠局灶性脑缺血再灌注损伤的保护作用研究

doi: 10.3969/j.issn.1006-0111.2018.01.007

1.
安徽中医药大学药学院, 安徽合肥 230012
2.
安徽中医药大学药学院, 安徽合肥 230012;上海市浦东新区浦南医院药剂科, 上海 200125
3.
第二军医大学药学院药理学教研室, 上海 200433

基金项目: 浦东新区卫生系统重要薄弱学科建设资助（PWZbr2017-16）

Protective effects of Fu-Yuan-Xing-Nao decoction for focal cerebral ischemia reperfusion injury in rats

1.
School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China
2.
School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China;Department of Pharmacy, Punan Hospital of Pudong New Area, Shanghai 200125, China
3.
Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

摘要: 目的探讨复元醒脑汤对大鼠局灶性脑缺血再灌注损伤的保护作用及其机制。方法将SPF级雄性SD大鼠随机分为假手术组、模型组、复元醒脑汤低、中、高（5.5、11、22 g/kg）剂量组。采用线栓法建立大鼠脑缺血再灌注损伤模型，缺血2 h再灌注24 h，对各组大鼠进行神经功能缺失评分，TTC染色测定脑梗死体积，HE染色观察脑组织病理变化，Nissl染色观察脑组织中尼氏小体的变化，Tunnel染色观察脑组织中神经细胞的凋亡情况。测定血清中的超氧化物歧化酶（SOD）及丙二醛（MDA）的水平。结果与模型组相比，复元醒脑汤低、中、高剂量组均可明显改善大脑中动脉缺血再灌注损伤大鼠的神经行为障碍；TTC染色结果显示，复元醒脑汤低、中、高剂量组大鼠脑梗死体积明显减小；HE结果显示给药组改善脑组织病理结构，Nissl染色结果表明给药组尼氏小体形态数量得到改善，Tunnel染色阳性细胞数减少，细胞凋亡率下降；与模型组相比，复元醒脑汤可提高脑缺血再灌注大鼠血清中SOD的水平，降低MDA水平。结论复元醒脑汤对大鼠大脑脑缺血再灌注损伤具有保护作用。
- 复元醒脑汤 /
- 缺血再灌注损伤 /
- 超氧化物歧化酶 /
- 丙二醛
Abstract: Objective To study the protective effect of Fu-Yuan-Xing-Nao decoction (FYXND) on rats with middle cerebral ischemia reperfusion injury. Methods Male Sprague-Dawley rats were randomly divided into sham, ischemia-reperfusion, and low, middle, high FYXND dose (5.5, 11, 22 g/kg) groups. Rats model was induced by 2 h of middle cerebral artery occlusion and 24 h reperfusion. The neurological deficit score of each group was evaluated. The infarct size was measured by the 2,3,5-triphenyltetra-zolium(TTC) chloride staining assay. The pathological changes of brain tissue were observed by HE staining assay. The changes of Nissl bodies were observed by Nissl staining. Tunnel staining was used to observe the apoptosis of neurons in brain. Serum levels of superoxide dismutase (SOD)and Malondialdehyde (MDA) were measured. Results Compared with the model group, neurological outcomes were improved in all three groups of low, middle and high FYXND dose (5.5, 11, 22 g/kg). Significantly reduced infarct brain volume was observed with TTC staining in all three FYXND groups. The results from HE staining assay indicated that the pathological structure of brain tissue was improved in the treatment groups. The numbers and morphology of Nissl corpuscles in the treated group were also improved based on the results of Nissl staining. Both the Tunnel staining positive cells and the rate of apoptosis were decreased. Compared with the model group, FYXND increased the rat serum SOD level and decreased the MDA level. Conclusion FYXND has protective effects on cerebral ischemia reperfusion injury in rats.
- Fu-Yuan-Xing-Nao decoction /
- ischemia reperfusion injury /
- SOD /
- MDA

[1]	Mozaffarian D,Benjamin EJ, Go AS, et al. Heart disease and stroke statistics——2015 update:a report from the American Heart Association[J]. Circulation,2015, 131(4):e29-322.
[2]	Mozaffarian D.Dietary and policy priorities for cardiovascular disease, diabetes, and obesity:a comprehensive review[J] Circulation,2016,133(2):187-225.
[3]	耿赟,方邦江,马智慧,等.复元醒脑汤治疗急性缺血性中风的临床研究[J].中国中医急症,2014,23(11):1970-1972.
[4]	赵平,沈俊逸,魏俊平,等.复元醒脑汤对糖尿病脑梗死大鼠脑组织梗死体积的影响[J].上海中医药大学学报,2013,27(5):66-69.
[5]	陈淼,王宏,方邦江.复元醒脑汤治疗糖尿病合并脑梗死的机制研究[J].中国急救医学,2013,33(7):654-657.
[6]	Woodruff TM,Thundyil J,Tang SC,et al. Pathophysiology, treatment, and animal and cellular models of human ischemic stroke[J]. Mol Neurodegener,2011,6(1):11.
[7]	Longa EZ,Weinstein PR,Carlson S,et al. Cummins Reversible middle cerebral artery occlusion without craniectomy in rats[J]. Stroke,1989,20(1):84-91.
[8]	Maki T,Hayakawa K,Pham L D,et al. Biphasic mechanisms of neurovascular unit injury and protection in CNS diseases[J].CNS Neurol Disord Drug Targets,2013,12(3):302-315.
[9]	Ayuso MI,Gonzalo-Gobernado R,Montaner J.Neuroprotective diets for stroke[J]. Neurochem Int,2017,107:4-10.
[10]	王立平,刘翠梅,张红.复元醒脑汤联合西药治疗腔隙性脑梗死疗效及对IL-6、TNF-α和NO的影响[J].现代中西医结合杂志,2015,24(29):3207-3208.
[11]	方邦江,周爽,沈俊逸,等. 复元醒脑汤对糖尿病脑梗死大鼠脑组织血流量及含水量影响的实验研究[J]. 老年医学与保健,2012,18(6):381-385.
[12]	沈俊逸,方邦江,凌丽,等.复元醒脑汤对糖尿病脑梗死大鼠内皮祖细胞功能的作用研究[J]. 中国中西医结合急救杂志,2016,23(4):412-416.

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复元醒脑汤对大鼠局灶性脑缺血再灌注损伤的保护作用研究

doi: 10.3969/j.issn.1006-0111.2018.01.007

Protective effects of Fu-Yuan-Xing-Nao decoction for focal cerebral ischemia reperfusion injury in rats

计量

复元醒脑汤对大鼠局灶性脑缺血再灌注损伤的保护作用研究

doi: 10.3969/j.issn.1006-0111.2018.01.007

1. 安徽中医药大学药学院, 安徽合肥 230012

2. 安徽中医药大学药学院, 安徽合肥 230012;上海市浦东新区浦南医院药剂科, 上海 200125

3. 第二军医大学药学院药理学教研室, 上海 200433

English Abstract

Protective effects of Fu-Yuan-Xing-Nao decoction for focal cerebral ischemia reperfusion injury in rats

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复元醒脑汤对大鼠局灶性脑缺血再灌注损伤的保护作用研究

doi: 10.3969/j.issn.1006-0111.2018.01.007

Protective effects of Fu-Yuan-Xing-Nao decoction for focal cerebral ischemia reperfusion injury in rats

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复元醒脑汤对大鼠局灶性脑缺血再灌注损伤的保护作用研究

doi: 10.3969/j.issn.1006-0111.2018.01.007

1. 安徽中医药大学药学院, 安徽 合肥 230012 2. 安徽中医药大学药学院, 安徽 合肥 230012;上海市浦东新区浦南医院药剂科, 上海 200125 3. 第二军医大学药学院药理学教研室, 上海 200433

English Abstract

Protective effects of Fu-Yuan-Xing-Nao decoction for focal cerebral ischemia reperfusion injury in rats

全文HTML

目录

1. 安徽中医药大学药学院, 安徽合肥 230012

2. 安徽中医药大学药学院, 安徽合肥 230012;上海市浦东新区浦南医院药剂科, 上海 200125

3. 第二军医大学药学院药理学教研室, 上海 200433