左卡尼汀缓解顺铂造成急性肾损伤的血清代谢组学研究

纪松岗; 吴琼; 朱臻宇; 董昕; 洪战英; 柴逸峰

doi:10.3969/j.issn.1006-0111.2015.05.012

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左卡尼汀缓解顺铂造成急性肾损伤的血清代谢组学研究

纪松岗, 吴琼, 朱臻宇, 董昕, 洪战英, 柴逸峰

文章导航 > 药学实践与服务 > 2015 > 33(5): 429-433

纪松岗, 吴琼, 朱臻宇, 董昕, 洪战英, 柴逸峰. 左卡尼汀缓解顺铂造成急性肾损伤的血清代谢组学研究[J]. 药学实践与服务, 2015, 33(5): 429-433. doi: 10.3969/j.issn.1006-0111.2015.05.012

引用本文:

JI Songgang, WU Qiong, ZHU Zhenyu, DONG Xin, HONG Zhanying, CHAI Yifeng. L-carnitine alleviating cisplatin induced acute kidney injury through serum metabolomics analysis[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(5): 429-433. doi: 10.3969/j.issn.1006-0111.2015.05.012

Citation:

JI Songgang, WU Qiong, ZHU Zhenyu, DONG Xin, HONG Zhanying, CHAI Yifeng. L-carnitine alleviating cisplatin induced acute kidney injury through serum metabolomics analysis[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(5): 429-433. doi: 10.3969/j.issn.1006-0111.2015.05.012

左卡尼汀缓解顺铂造成急性肾损伤的血清代谢组学研究

doi: 10.3969/j.issn.1006-0111.2015.05.012

1.
解放军401医院药剂科, 山东青岛 266071
2.
第二军医大学药学院, 上海 200433

基金项目: 自然科学基金面上项目(81273472);上海市中药现代化基金重点基金(12401900802)

L-carnitine alleviating cisplatin induced acute kidney injury through serum metabolomics analysis

1.
Department of Pharmacy, No.401 Hospital of PLA, Qingdao 266071, China
2.
School of Pharmacy, Second Military Medical University, Shanghai 200433, China

摘要: 目的利用血清代谢组学探究顺铂诱导小鼠急性肾损伤的特异性变量,同时评价左卡尼汀的干预作用。方法将19只小鼠分为正常对照组、模型组和干预组,适应3 d后,对干预组给予左卡尼汀(400 mg/kg,ip)干预,2 d后给予模型组和干预组顺铂(20 mg/kg,ip)造模,每天称量各组小鼠的体质量,2 d后取小鼠血清进行LC-MS分析,结合模式识别分析各组间代谢组差异,并评价左卡尼汀的干预作用。结果代谢组学分析共鉴别28个差异代谢物,顺铂诱导的急性肾损伤主要涉及磷脂类、氨基酸类和脂肪酸类代谢途径的改变,而左卡尼汀有改善作用。结论左卡尼汀可改善顺铂诱导的急性肾损伤,其机制可能是通过调控色氨酸代谢、谷氨酸代谢和能量代谢,从而减缓急性肾损伤的疾病进程。
- 左卡尼汀 /
- 顺铂 /
- 急性肾损伤 /
- 代谢组学
Abstract: Objective To explore specific variables related to cisplatin induced acute kidney injury, serum metabonomics techniques were applied and simultaneously the value of intervention effects of L-carnitine were appraised. Methods 19 mice were divided into the normal control group, model group, and intervention group, After a three day accommodation period, the intervention group was given L-carnitine (400 mg/kg, ip). Two days later, cisplatin (20 mg/kg, ip) was given to the model and intervention groups. The body weight of every mouse in each group was measured daily. Two days after the serum sample of each mice was collected and analyzed by LC-MS, pattern recognition analysis of metabolomics differences among the groups, and the effectiveness of L-carnitine intervention were evaluated. Results A total of 28 metabolites were identified through serum metabolomics analysis. Our data shows that there is a possible mechanism that cisplatin induced AKI was mainly involved in changing phospholipids, amino acid and fatty acid metabolic pathways and L-carnitine mitigates the damage of acute kidney injury induced by cisplatin. Conclusion L-carnitinecan alleviates cisplatin induced acute kidney injury by regulating tryptophan metabolism, glutamate metabolism, and energy metabolism.
- L-carnitine /
- cisplatin /
- acute kidney injury /
- metabolomics

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左卡尼汀缓解顺铂造成急性肾损伤的血清代谢组学研究

doi: 10.3969/j.issn.1006-0111.2015.05.012

1. 解放军401医院药剂科, 山东青岛 266071

2. 第二军医大学药学院, 上海 200433

基金项目: 自然科学基金面上项目(81273472);上海市中药现代化基金重点基金(12401900802)

收稿日期: 2014-11-27
修回日期: 2015-05-11

关键词:

左卡尼汀 /

顺铂 /

急性肾损伤 /

代谢组学

摘要: 目的利用血清代谢组学探究顺铂诱导小鼠急性肾损伤的特异性变量,同时评价左卡尼汀的干预作用。方法将19只小鼠分为正常对照组、模型组和干预组,适应3 d后,对干预组给予左卡尼汀(400 mg/kg,ip)干预,2 d后给予模型组和干预组顺铂(20 mg/kg,ip)造模,每天称量各组小鼠的体质量,2 d后取小鼠血清进行LC-MS分析,结合模式识别分析各组间代谢组差异,并评价左卡尼汀的干预作用。结果代谢组学分析共鉴别28个差异代谢物,顺铂诱导的急性肾损伤主要涉及磷脂类、氨基酸类和脂肪酸类代谢途径的改变,而左卡尼汀有改善作用。结论左卡尼汀可改善顺铂诱导的急性肾损伤,其机制可能是通过调控色氨酸代谢、谷氨酸代谢和能量代谢,从而减缓急性肾损伤的疾病进程。

English Abstract

L-carnitine alleviating cisplatin induced acute kidney injury through serum metabolomics analysis

1. Department of Pharmacy, No.401 Hospital of PLA, Qingdao 266071, China

2. School of Pharmacy, Second Military Medical University, Shanghai 200433, China

Received Date: 2014-11-27
Rev Recd Date: 2015-05-11

Keywords:

Abstract: Objective To explore specific variables related to cisplatin induced acute kidney injury, serum metabonomics techniques were applied and simultaneously the value of intervention effects of L-carnitine were appraised. Methods 19 mice were divided into the normal control group, model group, and intervention group, After a three day accommodation period, the intervention group was given L-carnitine (400 mg/kg, ip). Two days later, cisplatin (20 mg/kg, ip) was given to the model and intervention groups. The body weight of every mouse in each group was measured daily. Two days after the serum sample of each mice was collected and analyzed by LC-MS, pattern recognition analysis of metabolomics differences among the groups, and the effectiveness of L-carnitine intervention were evaluated. Results A total of 28 metabolites were identified through serum metabolomics analysis. Our data shows that there is a possible mechanism that cisplatin induced AKI was mainly involved in changing phospholipids, amino acid and fatty acid metabolic pathways and L-carnitine mitigates the damage of acute kidney injury induced by cisplatin. Conclusion L-carnitinecan alleviates cisplatin induced acute kidney injury by regulating tryptophan metabolism, glutamate metabolism, and energy metabolism.

引用本文:

Citation:

JI Songgang, WU Qiong, ZHU Zhenyu, DONG Xin, HONG Zhanying, CHAI Yifeng. L-carnitine alleviating cisplatin induced acute kidney injury through serum metabolomics analysis[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(5): 429-433. doi: 10.3969/j.issn.1006-0111.2015.05.012