绿萼梅总黄酮对慢性温和刺激所致抑郁大鼠神经内分泌和氧化应激的影响

陈静; 陈明珠; 黄玉香; 程晶; 黄雅平

doi:10.3969/j.issn.1006-0111.2019.03.007

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

x 关闭永久关闭

应中央军委要求，2022年9月起，《药学实践杂志》将更名为《药学实践与服务》，双月刊，正文96页；2023年1月起，拟出版月刊，正文64页，数据库收录情况与原《药学实践杂志》相同。欢迎作者踊跃投稿！

绿萼梅总黄酮对慢性温和刺激所致抑郁大鼠神经内分泌和氧化应激的影响

陈静, 陈明珠, 黄玉香, 程晶, 黄雅平

文章导航 > 药学实践与服务 > 2019 > 37(3): 226-230

陈静, 陈明珠, 黄玉香, 程晶, 黄雅平. 绿萼梅总黄酮对慢性温和刺激所致抑郁大鼠神经内分泌和氧化应激的影响[J]. 药学实践与服务, 2019, 37(3): 226-230. doi: 10.3969/j.issn.1006-0111.2019.03.007

引用本文:

CHEN Jing, CHEN Mingzhu, HUANG Yuxiang, CHENG Jing, HUANG Yaping. Effects of total flavonoids from Armeniaca mume on neuroendocrine and oxidative stress of rats with depression induced by chronic mild stress[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(3): 226-230. doi: 10.3969/j.issn.1006-0111.2019.03.007

Citation:

CHEN Jing, CHEN Mingzhu, HUANG Yuxiang, CHENG Jing, HUANG Yaping. Effects of total flavonoids from Armeniaca mume on neuroendocrine and oxidative stress of rats with depression induced by chronic mild stress[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(3): 226-230. doi: 10.3969/j.issn.1006-0111.2019.03.007

绿萼梅总黄酮对慢性温和刺激所致抑郁大鼠神经内分泌和氧化应激的影响

doi: 10.3969/j.issn.1006-0111.2019.03.007

泉州医学高等专科学校药理学教研室, 福建泉州 362000

基金项目: 福建省中青年教师教育科研项目（JAT160848）

Effects of total flavonoids from Armeniaca mume on neuroendocrine and oxidative stress of rats with depression induced by chronic mild stress

Department of Pharmacology, Quanzhou Medical College, Quanzhou 362000, China

摘要: 目的通过观察绿萼梅总黄酮（TFAM）对慢性温和刺激抑郁大鼠神经内分泌及氧化应激的影响，探讨其可能的抗抑郁机制。方法将SD大鼠随机分为6组：生理盐水组、模型组、阳性对照组（氟西汀，20 mg/kg）、低剂量治疗组（TD-TFAM，80 mg/kg）、中剂量治疗组（TZ-TFAM，160 mg/kg）和高剂量治疗组（TG-TFAM，240 mg/kg）；除生理盐水组外，其余各组大鼠经历28 d慢性不可预见性温和应激（CUMS）造模成功后随机分组。通过大鼠体重增量变化、糖水偏好实验、旷场实验评估TFAM的抗抑郁作用；采用酶联免疫吸附法（Elisa）测定大鼠海马和前额叶皮层去甲肾上腺素（NA）、5-羟色胺（5-HT）、促肾上腺皮质激素（ACTH）和皮质酮（CORT）含量，评估TFAM对神经递质表达量的影响；通过检测大鼠血清和纹状体中谷胱甘肽过氧化物酶（GSH-Px）和超氧化物歧化酶（SOD）的活性评估TFAM对氧化应激的影响。结果 TFAM能显著增加CUMS大鼠体重增值变量，提高糖水偏爱百分比，逆转旷场实验评分，提高大鼠海马和前额叶皮层NA、5-HT的含量，降低ACTH、CORT的含量，增强大鼠血清和纹状体中GSH-Px和SOD的活性。结论 TFAM抗抑郁作用可能与促进单胺递质分泌、调节下丘脑-垂体-肾上腺（HPA）轴功能和清除体内自由基有关。
- 绿萼梅总黄酮 /
- 抗抑郁 /
- 单胺神经递质 /
- 下丘脑-垂体-肾上腺轴 /
- 抗氧化
Abstract: Objective To explore the possible mechanism of total flavonoids from Armeniaca mume(TFAM) through observing its effects on neuroendocrine and oxidative stress of rats with depression induced by chronic mild stress. Methods The SD rats were randomly divided into normal saline group,model group,positive control group (fluoxetine,20 mg/kg),low-dose treatment group (TD-TFAM,80 mg/kg),middle-dose treatment group (TZ-TFAM,160 mg/kg) and high-dose treatment group (TG-TFAM,240 mg/kg),total 6 groups.Except the normal saline group,the rats in all other groups were given chronic unpredictable mild stress (CUMS) for 28 d to build the depression models,and then were randomly assigned.The antidepressant effects were evaluated by index such as growth in weight,sucrose preference and open-field test.Its effects on neuroendocrine were evaluated by contents of noradrenaline (NA),5-hydroxytryptamine (5-HT),adrenocorticotropic hormone (ACTH) and corticosterone (CORT) in the hippocampus and prefrontal cortex through enzyme linked immunosorbent assay (ELISA). Also,the enzymes activities of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in the serum and striatum were measured to estimate its influences on oxidative stress. Results TFAM significantly increased the weight growth of CUMS rats,promoted the percentage of sugar water preference,reversed open-field test score,increased the contents of 5-HT and NA but reduced the contents of ACTH and CORT in the hippocampus and prefrontal cortex of rats,while the activities of SOD and GSH-Px in serum and striatum were up-regulated. Conclusion The anti-depressant mechanism of TFAM might be related to promoting the secretion of monoamines,regulating the function of hypothalamic-pituitary-adrenal (HPA) axis and removing free radicals in rats.
- total flavonoids from Armeniaca mume /
- depression /
- monoamine neurotransmitter /
- hypothalamic-pituitary-adrenal /
- HPA

[1]	ALEMAN A,DENYS D.Mental health:a road map for suicide research and prevention[J].Nature,2014,509(7501):421-423.
[2]	慕磊,孙建绪.抑郁症发病机制与抗抑郁药物作用靶标[J].国际药学研究杂志,2015,42(4):463-466.
[3]	龚金炎,吴晓琴,毛建卫,等.黄酮类化合物抗抑郁作用的研究进展[J].中草药,2011,42(1):195-200.
[4]	江苏新医学院.中药大辞典(上册)[M].上海:上海科学技术出版社,1997:735.
[5]	张清华,张玲,尚立霞,等.白梅花的化学成分研究[J].中药材,2008,31(11):1666-1668.
[6]	高洪元,田青.佛手挥发油的抗抑郁作用机制探讨[J].中国实验方剂学杂志,2012,18(7):231-234.
[7]	陈明珠,陈静,黄幼霞,等.AB-8型大孔吸附树脂分离纯化绿萼梅总黄酮[J].海峡药学,2017,29(2):38-41.
[8]	游杰舒,张瑞睿,郭建友,等.哈蟆油石油醚提取物的抗抑郁作用及其机制[J].中草药,2013(19):2717-2721.
[9]	徐颖,冯劼,郭建友.枳壳提取物抗抑郁作用及其机制探讨[J].中国临床药理学与治疗学,2013,18(10):1086-1092.
[10]	李连玲,刘晓丽,袁靖姣,等.抑郁模型的研究进展[J].中国健康心理学杂志,2014(10):1588-1590.
[11]	李晓秋,许晶.抑郁动物模型的研究进展[J].中华精神科杂志,2002, 35(3):184-186.
[12]	韩晓蕾,胡风云,田峰,等.抑郁动物模型的研究进展及评价[J].临床医药实践,2015,24(7):533-536.
[13]	沈双宏,胡随瑜,唐凤英,等.复方刺蒺藜苷对慢性应激抑郁模型大鼠行为学及海马CNTF表达的影响[J].中国临床心理学杂志,2006,14(3):278-280.
[14]	孙达亮,李雪,刘庆贺.电针结合氟西汀对抑郁模型大鼠行为学及HPA轴的影响[J].精神医学杂志,2016,29(1):4-8.
[15]	张峰,曹仲伟,张学杰,等.柴胡对大鼠慢性应激抑郁模型脑单胺类神经递质及其代谢物含量的影响[J].山东中医药大学学报,2005,29(3):224-226.
[16]	李红娅,石延榜,蒋艳玲.丹栀逍遥散胶囊对慢性应激抑郁模型下丘脑单胺类神经递质的影响[J].中国医药导报,2007,4(30):61-62.
[17]	唐卓君,邹伟.抗抑郁药的研究进展[J].四川精神卫生,2014(6):569-570.
[18]	叶光明,姜云云,瞿发林,等.抗抑郁药的研究进展[J].中国药师,2006(10):954-956.
[19]	曹莉莎,叶云,罗文,等.抗抑郁药物作用靶点的研究进展[J].中国药房,2014(13):1227-1231.
[20]	呼日乐巴根,松林,阿拉木斯,等.肉蔻-5味丸对慢性抑郁模型大鼠血清ACTH和CORT含量的影响[J].中国民族医药杂志,2015,21(3):47-49.
[21]	武丽,张丽萍,陈贵海,等.解郁1号对抑郁大鼠血浆促肾上腺皮质激素释放因子和皮质醇含量的影响[J].时珍国医国药,2009,20(2):58-60.
[22]	付晓菲,徐风华,陈建,等.酰米菲肽对慢性应激模型大鼠的抗抑郁作用[J].军事医学,2014,38(4):281-284.
[23]	VAVAKOVA M,DURACKOVA Z,TREBATICKA J.Markers of oxidative stress and neuroprogression in depression disorder[J].Oxid Med Cell Longev,2015,2015:898393.
[24]	王玉龙,刘廷毅,董海燕,等.抑郁模型大鼠大脑和结肠中SOD、MDA和NO含量的异常[J].中华行为医学与脑科学杂志,2005,14(7):627-628.
[25]	邱海鹏,张晓璇,申兴斌,等.补肾益智方联合丁苯酞对阿尔茨海默病患者Aβ,GSH-Px,SOD及MDA水平的影响[J].中国实验方剂学杂志,2016,22(14):187-191.

[1]	郭灵怡, 刘艳超, 高路, 刘瑞瑶, 吕权真, 俞媛. 醋酸卡泊芬净单硬脂酸甘油酯纳米粒抗白色念珠菌感染的增效作用研究 . 药学实践与服务, 2024, 42(): 1-8. doi: 10.12206/j.issn.2097-2024.202310043
[2]	冯志惠, 邓仪卿, 叶冰, 安培, 张宏, 张海军. 雀梅藤石油醚提取物诱导三阴性乳腺癌细胞凋亡的实验研究 . 药学实践与服务, 2024, 42(6): 253-259. doi: 10.12206/j.issn.2097-2024.202311055
[3]	陈方剑, 骆锦前, 王志君, 胡叶帅, 孙煜昕, 宋洪杰. HPLC-MS/MS同时测定感冒安颗粒中5种黄酮成分的含量 . 药学实践与服务, 2024, 42(9): 402-406. doi: 10.12206/j.issn.2097-2024.202403030
[4]	修建平, 杨朝爱, 刘禧澳, 潘乾禹, 韦广旭, 王卫星. 全反式维甲酸对肝星状细胞活化及氧化应激的作用和机制探索 . 药学实践与服务, 2024, 42(7): 291-296. doi: 10.12206/j.issn.2097-2024.202312054
[5]	岳春华, 贲永光, 王海桥. 基于NLRP1炎症小体探讨百合知母汤抗抑郁的作用机制 . 药学实践与服务, 2024, 42(8): 325-333. doi: 10.12206/j.issn.2097-2024.202401033
[6]	赖立勇, 夏天爽, 徐圣焱, 蒋益萍, 岳小强, 辛海量. 中药青蒿抗氧化活性的谱效关系研究 . 药学实践与服务, 2024, 42(5): 203-210, 216. doi: 10.12206/j.issn.2097-2024.202211012

点击查看大图

计量

文章访问数: 3594
HTML全文浏览量: 442
PDF下载量: 395
被引次数: 0

绿萼梅总黄酮对慢性温和刺激所致抑郁大鼠神经内分泌和氧化应激的影响

doi: 10.3969/j.issn.1006-0111.2019.03.007

泉州医学高等专科学校药理学教研室, 福建泉州 362000

基金项目: 福建省中青年教师教育科研项目（JAT160848）

收稿日期: 2018-06-28
修回日期: 2019-01-20

关键词:

抗抑郁 /

摘要: 目的通过观察绿萼梅总黄酮（TFAM）对慢性温和刺激抑郁大鼠神经内分泌及氧化应激的影响，探讨其可能的抗抑郁机制。方法将SD大鼠随机分为6组：生理盐水组、模型组、阳性对照组（氟西汀，20 mg/kg）、低剂量治疗组（TD-TFAM，80 mg/kg）、中剂量治疗组（TZ-TFAM，160 mg/kg）和高剂量治疗组（TG-TFAM，240 mg/kg）；除生理盐水组外，其余各组大鼠经历28 d慢性不可预见性温和应激（CUMS）造模成功后随机分组。通过大鼠体重增量变化、糖水偏好实验、旷场实验评估TFAM的抗抑郁作用；采用酶联免疫吸附法（Elisa）测定大鼠海马和前额叶皮层去甲肾上腺素（NA）、5-羟色胺（5-HT）、促肾上腺皮质激素（ACTH）和皮质酮（CORT）含量，评估TFAM对神经递质表达量的影响；通过检测大鼠血清和纹状体中谷胱甘肽过氧化物酶（GSH-Px）和超氧化物歧化酶（SOD）的活性评估TFAM对氧化应激的影响。结果 TFAM能显著增加CUMS大鼠体重增值变量，提高糖水偏爱百分比，逆转旷场实验评分，提高大鼠海马和前额叶皮层NA、5-HT的含量，降低ACTH、CORT的含量，增强大鼠血清和纹状体中GSH-Px和SOD的活性。结论 TFAM抗抑郁作用可能与促进单胺递质分泌、调节下丘脑-垂体-肾上腺（HPA）轴功能和清除体内自由基有关。

English Abstract

Effects of total flavonoids from Armeniaca mume on neuroendocrine and oxidative stress of rats with depression induced by chronic mild stress

Department of Pharmacology, Quanzhou Medical College, Quanzhou 362000, China

Received Date: 2018-06-28
Rev Recd Date: 2019-01-20

Keywords:

total flavonoids from Armeniaca mume /
depression /
monoamine neurotransmitter /
hypothalamic-pituitary-adrenal /
HPA

Abstract: Objective To explore the possible mechanism of total flavonoids from Armeniaca mume(TFAM) through observing its effects on neuroendocrine and oxidative stress of rats with depression induced by chronic mild stress. Methods The SD rats were randomly divided into normal saline group,model group,positive control group (fluoxetine,20 mg/kg),low-dose treatment group (TD-TFAM,80 mg/kg),middle-dose treatment group (TZ-TFAM,160 mg/kg) and high-dose treatment group (TG-TFAM,240 mg/kg),total 6 groups.Except the normal saline group,the rats in all other groups were given chronic unpredictable mild stress (CUMS) for 28 d to build the depression models,and then were randomly assigned.The antidepressant effects were evaluated by index such as growth in weight,sucrose preference and open-field test.Its effects on neuroendocrine were evaluated by contents of noradrenaline (NA),5-hydroxytryptamine (5-HT),adrenocorticotropic hormone (ACTH) and corticosterone (CORT) in the hippocampus and prefrontal cortex through enzyme linked immunosorbent assay (ELISA). Also,the enzymes activities of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in the serum and striatum were measured to estimate its influences on oxidative stress. Results TFAM significantly increased the weight growth of CUMS rats,promoted the percentage of sugar water preference,reversed open-field test score,increased the contents of 5-HT and NA but reduced the contents of ACTH and CORT in the hippocampus and prefrontal cortex of rats,while the activities of SOD and GSH-Px in serum and striatum were up-regulated. Conclusion The anti-depressant mechanism of TFAM might be related to promoting the secretion of monoamines,regulating the function of hypothalamic-pituitary-adrenal (HPA) axis and removing free radicals in rats.

引用本文: