Effects of total flavonoids from Armeniaca mume on neuroendocrine and oxidative stress of rats with depression induced by chronic mild stress
-
摘要: 目的 通过观察绿萼梅总黄酮（TFAM）对慢性温和刺激抑郁大鼠神经内分泌及氧化应激的影响，探讨其可能的抗抑郁机制。 方法 将SD大鼠随机分为6组：生理盐水组、模型组、阳性对照组（氟西汀，20 mg/kg）、低剂量治疗组（TD-TFAM，80 mg/kg）、中剂量治疗组（TZ-TFAM，160 mg/kg）和高剂量治疗组（TG-TFAM，240 mg/kg）；除生理盐水组外，其余各组大鼠经历28 d慢性不可预见性温和应激（CUMS）造模成功后随机分组。通过大鼠体重增量变化、糖水偏好实验、旷场实验评估TFAM的抗抑郁作用；采用酶联免疫吸附法（Elisa）测定大鼠海马和前额叶皮层去甲肾上腺素（NA）、5-羟色胺（5-HT）、促肾上腺皮质激素（ACTH）和皮质酮（CORT）含量，评估TFAM对神经递质表达量的影响；通过检测大鼠血清和纹状体中谷胱甘肽过氧化物酶（GSH-Px）和超氧化物歧化酶（SOD）的活性评估TFAM对氧化应激的影响。 结果 TFAM能显著增加CUMS大鼠体重增值变量，提高糖水偏爱百分比，逆转旷场实验评分，提高大鼠海马和前额叶皮层NA、5-HT的含量，降低ACTH、CORT的含量，增强大鼠血清和纹状体中GSH-Px和SOD的活性。 结论 TFAM抗抑郁作用可能与促进单胺递质分泌、调节下丘脑-垂体-肾上腺（HPA）轴功能和清除体内自由基有关。
-
关键词:
- 绿萼梅总黄酮 /
- 抗抑郁 /
- 单胺神经递质 /
- 下丘脑-垂体-肾上腺轴 /
- 抗氧化
Abstract: Objective To explore the possible mechanism of total flavonoids from Armeniaca mume(TFAM) through observing its effects on neuroendocrine and oxidative stress of rats with depression induced by chronic mild stress. Methods The SD rats were randomly divided into normal saline group,model group,positive control group (fluoxetine,20 mg/kg),low-dose treatment group (TD-TFAM,80 mg/kg),middle-dose treatment group (TZ-TFAM,160 mg/kg) and high-dose treatment group (TG-TFAM,240 mg/kg),total 6 groups.Except the normal saline group,the rats in all other groups were given chronic unpredictable mild stress (CUMS) for 28 d to build the depression models,and then were randomly assigned.The antidepressant effects were evaluated by index such as growth in weight,sucrose preference and open-field test.Its effects on neuroendocrine were evaluated by contents of noradrenaline (NA),5-hydroxytryptamine (5-HT),adrenocorticotropic hormone (ACTH) and corticosterone (CORT) in the hippocampus and prefrontal cortex through enzyme linked immunosorbent assay (ELISA). Also,the enzymes activities of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in the serum and striatum were measured to estimate its influences on oxidative stress. Results TFAM significantly increased the weight growth of CUMS rats,promoted the percentage of sugar water preference,reversed open-field test score,increased the contents of 5-HT and NA but reduced the contents of ACTH and CORT in the hippocampus and prefrontal cortex of rats,while the activities of SOD and GSH-Px in serum and striatum were up-regulated. Conclusion The anti-depressant mechanism of TFAM might be related to promoting the secretion of monoamines,regulating the function of hypothalamic-pituitary-adrenal (HPA) axis and removing free radicals in rats. -
[1] ALEMAN A,DENYS D.Mental health:a road map for suicide research and prevention[J].Nature,2014,509(7501):421-423. [2] 慕磊,孙建绪.抑郁症发病机制与抗抑郁药物作用靶标[J].国际药学研究杂志,2015,42(4):463-466. [3] 龚金炎,吴晓琴,毛建卫,等.黄酮类化合物抗抑郁作用的研究进展[J].中草药,2011,42(1):195-200. [4] 江苏新医学院.中药大辞典(上册)[M].上海:上海科学技术出版社,1997:735. [5] 张清华,张玲,尚立霞,等.白梅花的化学成分研究[J].中药材,2008,31(11):1666-1668. [6] 高洪元,田青.佛手挥发油的抗抑郁作用机制探讨[J].中国实验方剂学杂志,2012,18(7):231-234. [7] 陈明珠,陈静,黄幼霞,等.AB-8型大孔吸附树脂分离纯化绿萼梅总黄酮[J].海峡药学,2017,29(2):38-41. [8] 游杰舒,张瑞睿,郭建友,等.哈蟆油石油醚提取物的抗抑郁作用及其机制[J].中草药,2013(19):2717-2721. [9] 徐颖,冯劼,郭建友.枳壳提取物抗抑郁作用及其机制探讨[J].中国临床药理学与治疗学,2013,18(10):1086-1092. [10] 李连玲,刘晓丽,袁靖姣,等.抑郁模型的研究进展[J].中国健康心理学杂志,2014(10):1588-1590. [11] 李晓秋,许晶.抑郁动物模型的研究进展[J].中华精神科杂志,2002, 35(3):184-186. [12] 韩晓蕾,胡风云,田峰,等.抑郁动物模型的研究进展及评价[J].临床医药实践,2015,24(7):533-536. [13] 沈双宏,胡随瑜,唐凤英,等.复方刺蒺藜苷对慢性应激抑郁模型大鼠行为学及海马CNTF表达的影响[J].中国临床心理学杂志,2006,14(3):278-280. [14] 孙达亮,李雪,刘庆贺.电针结合氟西汀对抑郁模型大鼠行为学及HPA轴的影响[J].精神医学杂志,2016,29(1):4-8. [15] 张峰,曹仲伟,张学杰,等.柴胡对大鼠慢性应激抑郁模型脑单胺类神经递质及其代谢物含量的影响[J].山东中医药大学学报,2005,29(3):224-226. [16] 李红娅,石延榜,蒋艳玲.丹栀逍遥散胶囊对慢性应激抑郁模型下丘脑单胺类神经递质的影响[J].中国医药导报,2007,4(30):61-62. [17] 唐卓君,邹伟.抗抑郁药的研究进展[J].四川精神卫生,2014(6):569-570. [18] 叶光明,姜云云,瞿发林,等.抗抑郁药的研究进展[J].中国药师,2006(10):954-956. [19] 曹莉莎,叶云,罗文,等.抗抑郁药物作用靶点的研究进展[J].中国药房,2014(13):1227-1231. [20] 呼日乐巴根,松林,阿拉木斯,等.肉蔻-5味丸对慢性抑郁模型大鼠血清ACTH和CORT含量的影响[J].中国民族医药杂志,2015,21(3):47-49. [21] 武丽,张丽萍,陈贵海,等.解郁1号对抑郁大鼠血浆促肾上腺皮质激素释放因子和皮质醇含量的影响[J].时珍国医国药,2009,20(2):58-60. [22] 付晓菲,徐风华,陈建,等.酰米菲肽对慢性应激模型大鼠的抗抑郁作用[J].军事医学,2014,38(4):281-284. [23] VAVAKOVA M,DURACKOVA Z,TREBATICKA J.Markers of oxidative stress and neuroprogression in depression disorder[J].Oxid Med Cell Longev,2015,2015:898393. [24] 王玉龙,刘廷毅,董海燕,等.抑郁模型大鼠大脑和结肠中SOD、MDA和NO含量的异常[J].中华行为医学与脑科学杂志,2005,14(7):627-628. [25] 邱海鹏,张晓璇,申兴斌,等.补肾益智方联合丁苯酞对阿尔茨海默病患者Aβ,GSH-Px,SOD及MDA水平的影响[J].中国实验方剂学杂志,2016,22(14):187-191. -
点击查看大图
计量
- 文章访问数: 5267
- HTML全文浏览量: 635
- PDF下载量: 403
- 被引次数: 0
下载:
