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秀丽隐杆线虫在抗感染研究中的应用

胡淦海,  李德东,  赵兰雪,  王彦,  姜远英

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胡淦海, 李德东, 赵兰雪, 王彦, 姜远英. 秀丽隐杆线虫在抗感染研究中的应用[J]. 药学实践与服务, 2014, 32(1): 5-8. doi: 10.3969/j.issn.1006-0111.2014.01.002
引用本文: 胡淦海, 李德东, 赵兰雪, 王彦, 姜远英. 秀丽隐杆线虫在抗感染研究中的应用[J]. 药学实践与服务, 2014, 32(1): 5-8. doi: 10.3969/j.issn.1006-0111.2014.01.002
shu
HU Ganhai, LI Dedong, ZHAO Lanxue, WANG Yan, JIANG Yuanying. Application of Caenorhabditis elegans in anti-infective research[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(1): 5-8. doi: 10.3969/j.issn.1006-0111.2014.01.002
Citation: HU Ganhai, LI Dedong, ZHAO Lanxue, WANG Yan, JIANG Yuanying. Application of Caenorhabditis elegans in anti-infective research[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(1): 5-8. doi: 10.3969/j.issn.1006-0111.2014.01.002
shu

秀丽隐杆线虫在抗感染研究中的应用

doi: 10.3969/j.issn.1006-0111.2014.01.002
  • 1.

    福建中医药大学药学院中药学教研室, 福建 福州 350108;第二军医大学药学院新药研究中心, 上海 200433

  • 2.

    第二军医大学药学院新药研究中心, 上海 200433

基金项目: 国家自然科学基金(81273558,81072678,90913008);国家重点基础研究发展计划(2013CB531602);国家科技部科技重大专项(2011ZX09102-002-01);上海市科技重点项目(10431902200).

Application of Caenorhabditis elegans in anti-infective research

  • 1.

    School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, 350108 China;New Drug Research and Development Center, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

  • 2.

    New Drug Research and Development Center, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

  • 摘要: 目的 介绍秀丽隐杆线虫(Caenorhabditis elegans)作为模式生物宿主在抗感染研究中的应用,为秀丽隐杆线虫在抗感染研究领域的进一步应用提供参考。 方法 参阅近年来国内、外相关文献,对其进行分析、整合及归纳。 结果 发现秀丽隐杆线虫具有生长周期短、成本低等特点,被广泛用于病原微生物致病机制的研究以及抗感染药物的研发。 结论 秀丽隐杆线虫在病原微生物致病机制研究和抗感染药物研发中有广阔的应用前景。
    Abstract: Objective To update the application of Caenorhabditis elegans as a model host of infection in anti-infection research field, and provide references for the further application of C. elegans in the research field. Methods The related literatures at home and abroad in recent years were analyzed, integrated and concluded. Results C. elegans had been widely used to study pathogenesis of microorganisms and to screen anti-infective agents, since the nemades had short life cycle and cost low. Conclusion C. elegans would be promising to be further more widely used to study pathogenesis and anti-infective agents.
  • [1] Millet A,Ewbank JJ. Immunity in Caenorhabditis elegans[J]. Curr Opin Immunol, 2004, 16(1):4-9.
    [2] Ferrandon D, Imler JL, Hetru C, et al. The drosophila systemic immune response:sensing and signalling during bacterial and fungal infections[J]. Nat Rev Immunol, 2007, 7(11):862-874.
    [3] Trede NS, Langenau DM, Traver D, et al. The use of zebra fish to understand immunity[J]. Immunity, 2004, 20(4):367-379.
    [4] Brenner S. The genetics of Caenorhabditis elegans[J]. Genetics, 1974, 77(1):71-94.
    [5] Byerly L,Cassada R,Russell R. The life cycle of the nematode Caenorhabditis elegans:I. Wild-type growth and reproduction[J]. Devel Biol, 1976, 51(1):23-33.
    [6] Cassada RC,Russell RL. The dauer larva, a post-embryonic developmental variant of the nematode Caenorhabditis elegans[J]. Devel Biol, 1975, 46(2):326-342.
    [7] Albert PS,Brown SJ,Riddle DL. Sensory control of dauer larva formation in Caenorhabditis elegans[J]. J Compar Neur, 1981, 198(3):435-451.
    [8] Sifri CD,Begun J,Ausubel FM. The worm has turned-microbial virulence modeled in Caenorhabditis elegans[J]. Trends Microbiol, 2005, 13(3):119-127.
    [9] Lindsay JA. Genomic variation and evolution of Staphylococcus aureus[J]. Intern J Med Microbiol, 2010, 300(2):98-103.
    [10] Garsin DA, Sifri CD, Mylonakis E, et al. A simple model host for identifying Gram-positive virulence factors[J]. Proc Natl Acad Sci, 2001, 98(19):10892-10897.
    [11] Irazoqui JE, Troemel ER, Feinbaum RL, et al. Distinct pathogenesis and host responses during infection of C. elegans by P. aeruginosa and S. aureus[J]. PLo S Pathogens, 2010, 6(7):1-24.
    [12] Sifri CD, Begun J, Ausubel FM, et al. Caenorhabditis elegans as a model host for Staphylococcus aureus pathogenesis[J]. Infect Immun, 2003, 71(4):2208-2217.
    [13] Ogawa T, Sato M, Yonekawa S, et al. Infective endocarditis caused by enterococcus faecalis treated with continuous infusion of ampicillin without adjunctive aminoglycosides[J]. Intern Med, 2012, 52(10):1131-1135.
    [14] Maadani A, Fox KA, Mylonakis E, et al. Enterococcus faecalis mutations affecting virulence in the Caenorhabditis elegans model host[J]. Infect Immun, 2007, 75(5):2634-2637.
    [15] Sifri CD, Mylonakis E, Singh KV, et al. Virulence effect of Enterococcus faecalis protease genes and the quorum-sensing locus fsr in Caenorhabditis elegans and mice[J]. Infect Immun, 2002, 70(10):5647-5650.
    [16] Chávez V, Mohri-Shiomi A, Maadani A, et al. Oxidative stress enzymes are required for DAF-16-mediated immunity due to generation of reactive oxygen species by Caenorhabditis elegans[J]. Genetics, 2007, 176(3):1567-1577.
    [17] van der Hoeven R, McCallum KC, Cruz M R, et al. Ce-Duox1/BLI-3 generated reactive oxygen species trigger protective SKN-1 activity via p38 MAPK signaling during infection in C. elegans[J]. PLoS Pathogens, 2011, 7(12):1-14.
    [18] Mahajan-Miklos S, Tan MW, Rahme LG, et al. Molecular mechanisms of bacterial virulence elucidated using a Pseudomonas aeruginosa-Caenorhabditis elegans.Pathogene Model[J]. Cell, 1999, 96(1):47-56.
    [19] Tan MW,Mahajan-Miklos S,Ausubel F M. Killing of Caenorhabditis elegans by Pseudomonas aeruginosa used to model mammalian bacterial pathogenesis[J]. Proc Natl Acad Sci, 1999, 96(2):715-720.
    [20] Kabir MA,Hussain MA. Human fungal pathogen Candida albicans in the postgenomic era:an overview[J]. Expert Rev Anti-infect Ther, 2009, 7(1):121-134.
    [21] Breger J, Fuchs B B, Aperis G, et al. Antifungal chemical compounds identified using a C. elegans pathogenicity assay[J]. PLoS Pathogens, 2007, 3(2):0168-0178.
    [22] Mayer FL,Wilson D,Hube B. Candida albicans pathogenicity mechanisms[J]. Virulence, 2013, 4(2):119-128.
    [23] Pukkila-Worley R,Ausubel F M,Mylonakis E. Candida albicans infection of Caenorhabditis elegans induces antifungal immune defenses[J]. PLoS Pathogens, 2011, 7(6):1-13.
    [24] Pukkila-Worley R, Peleg AY, Tampakakis E, et al. Candida albicans hyphal formation and virulence assessed using a Caenorhabditis elegans infection model[J]. Eukary cell, 2009, 8(11):1750-1758.
    [25] Pukkila-Worley R,Mylonakis E. From the outside in and the inside out:antifungal immune responses in Caenorhabditis elegans[J]. Virulence, 2010, 1(3):111-112.
    [26] Gantner BN,Simmons RM,Underhill DM. Dectin-1 mediates macrophage recognition of Candida albicans yeast but not filaments[J]. EMBO J, 2005, 24(6):1277-1286.
    [27] Netea MG, Brown GD, Kullberg BJ, et al. An integrated model of the recognition of Candida albicans by the innate immune system[J]. Nat Rev Microbiol, 2008, 6(1):67-78.
    [28] Jouault T, Sarazin A, Martinez-Esparza M, et al. Host responses to a versatile commensal:PAMPs and PRRs interplay leading to tolerance or infection by Candida albicans[J]. Cellular Microbiol, 2009, 11(7):1007-1015.
    [29] Peleg AY, Tampakakis E, Fuchs BB, et al. Prokaryote-eukaryote interactions identified by using Caenorhabditis elegans[J]. Proc Natl Acad Sci, 2008, 105(38):14585-14590.
    [30] Tampakakis E,Peleg AY,Mylonakis E. Interaction of Candida albicans with an intestinal pathogen, Salmonella enterica serovar Typhimurium[J]. Eukary Cell, 2009, 8(5):732-737.
    [31] Mylonakis E, Ausubel FM, Perfect JR, et al. Killing of Caenorhabditis elegans by Cryptococcus neoformans as a model of yeast pathogenesis[J].Proc Natl Acad Sci, 2002, 99(24):15675-15680.
    [32] van den Berg MC, Woerlee JZ, Ma H, et al. Sex-dependent resistance to the pathogenic fungus Cryptococcus neoformans[J]. Genetics, 2006, 173(2):677-683.
    [33] Tang RJ, Breger J, Idnurm A, et al. Cryptococcus neoformans gene involved in mammalian pathogenesis identified by a Caenorhabditis elegans progeny-based approach[J]. Infect Immun, 2005, 73(12):8219-6225.
    [34] Powell JR,Ausubel FM. Models of Caenorhabditis elegans infection by bacterial and fungal pathogens[J].Meth Molecul Biol, 2008, 415:403-427.
    [35] Moy T I, Ball A R, Anklesaria Z, et al. Identification of novel antimicrobials using a live-animal infection model[J]. Proc Natl Acad Sci, 2006, 103(27):10414-10419.
    [36] Moy TI, Conery AL, Larkins-Ford J, et al. High-throughput screen for novel antimicrobials using a whole animal infection model[J]. ACS Chem Biol, 2009, 4(7):527-533.
    [37] Zhou YM, Shao L, Li JA, et al. An efficient and novel screening model for assessing the bioactivity of extracts against multidrug-resistant Pseudomonas aeruginosa using Caenorhabditis elegans[J]. Biosci Biotechnol Biochem, 2011, 75(9):1746-1751.
    [38] Okoli I, Coleman J J, Tempakakis E, et al. Identification of antifungal compounds active against Candida albicans using an improved high-throughput Caenorhabditis elegans assay[J]. PloS One, 2009, 4(9):1-8.
    [39] Coleman JJ, Okoli I, Tegos GP, et al. Characterization of plant-derived saponin natural products against Candida albicans[J]. ACS Chem Biol, 2010, 5(3):321-332.
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  • 收稿日期:  2013-07-04
  • 修回日期:  2013-11-17

秀丽隐杆线虫在抗感染研究中的应用

doi: 10.3969/j.issn.1006-0111.2014.01.002
  • 1. 福建中医药大学药学院中药学教研室, 福建 福州 350108;第二军医大学药学院新药研究中心, 上海 200433
  • 2. 第二军医大学药学院新药研究中心, 上海 200433
    • 基金项目:  国家自然科学基金(81273558,81072678,90913008);国家重点基础研究发展计划(2013CB531602);国家科技部科技重大专项(2011ZX09102-002-01);上海市科技重点项目(10431902200).
  • 收稿日期: 2013-07-04
  • 修回日期: 2013-11-17

摘要: 目的 介绍秀丽隐杆线虫(Caenorhabditis elegans)作为模式生物宿主在抗感染研究中的应用,为秀丽隐杆线虫在抗感染研究领域的进一步应用提供参考。 方法 参阅近年来国内、外相关文献,对其进行分析、整合及归纳。 结果 发现秀丽隐杆线虫具有生长周期短、成本低等特点,被广泛用于病原微生物致病机制的研究以及抗感染药物的研发。 结论 秀丽隐杆线虫在病原微生物致病机制研究和抗感染药物研发中有广阔的应用前景。

English Abstract

胡淦海, 李德东, 赵兰雪, 王彦, 姜远英. 秀丽隐杆线虫在抗感染研究中的应用[J]. 药学实践与服务, 2014, 32(1): 5-8. doi: 10.3969/j.issn.1006-0111.2014.01.002
引用本文: 胡淦海, 李德东, 赵兰雪, 王彦, 姜远英. 秀丽隐杆线虫在抗感染研究中的应用[J]. 药学实践与服务, 2014, 32(1): 5-8. doi: 10.3969/j.issn.1006-0111.2014.01.002
shu
HU Ganhai, LI Dedong, ZHAO Lanxue, WANG Yan, JIANG Yuanying. Application of Caenorhabditis elegans in anti-infective research[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(1): 5-8. doi: 10.3969/j.issn.1006-0111.2014.01.002
Citation: HU Ganhai, LI Dedong, ZHAO Lanxue, WANG Yan, JIANG Yuanying. Application of Caenorhabditis elegans in anti-infective research[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(1): 5-8. doi: 10.3969/j.issn.1006-0111.2014.01.002
shu

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