LC-MS/MS测定大鼠血浆中虎杖苷浓度

丁雪鹰; 侯晓丽; 孙铭学; 肖凯

doi:10.3969/j.issn.1006-0111.2012.01.012

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

x 关闭永久关闭

重要提示

《药学实践与服务》杂志不接收生物战剂、毒剂相关内容的稿件。对于涉军信息类论文如需投稿，请务必投稿前电话或邮箱咨询。联系电话：021-81871323，邮箱：yxsjzzs@163.com。敬请谅解，谢谢配合！

《药学实践与服务》编辑部

重要通知

《药学实践与服务》杂志目前不收取审稿费、版面费、加急费等费用，如收到邮件声称是编辑部X编辑，要求加作者微信的，或填写编委登记申请表的，请谨防诈骗！

编辑部用于作者校稿时微信绑定的邮件是通过官方邮箱：yxsjzzs@163.com发送的，标题是《药学实践与服务》XML数字出版服务微信绑定，请区分开！如有疑虑，请联系编辑部，电话：021-81871323。

《药学实践与服务》编辑部

LC-MS/MS测定大鼠血浆中虎杖苷浓度

丁雪鹰, 侯晓丽, 孙铭学, 肖凯

文章导航 > 药学实践与服务 > 2012 > 30(1): 45-48

丁雪鹰, 侯晓丽, 孙铭学, 肖凯. LC-MS/MS测定大鼠血浆中虎杖苷浓度[J]. 药学实践与服务, 2012, 30(1): 45-48. doi: 10.3969/j.issn.1006-0111.2012.01.012

引用本文:

丁雪鹰, 侯晓丽, 孙铭学, 肖凯. LC-MS/MS测定大鼠血浆中虎杖苷浓度[J]. 药学实践与服务, 2012, 30(1): 45-48. doi: 10.3969/j.issn.1006-0111.2012.01.012

DING Xue-ying, HOU Xiao-li, SUN Ming-xue, XIAO Kai. Determination of polydatin in rats plasma by LC-MS/MS[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(1): 45-48. doi: 10.3969/j.issn.1006-0111.2012.01.012

Citation:

DING Xue-ying, HOU Xiao-li, SUN Ming-xue, XIAO Kai. Determination of polydatin in rats plasma by LC-MS/MS[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(1): 45-48. doi: 10.3969/j.issn.1006-0111.2012.01.012

LC-MS/MS测定大鼠血浆中虎杖苷浓度

doi: 10.3969/j.issn.1006-0111.2012.01.012

1.
第二军医大学药学院药剂学教研室,上海 200433
2.
第二军医大学海医系毒物药物研究室,上海 200433

基金项目: 上海市科委中药现代化项目(08DZ1971504);国家自然科学基金(20872179,30472141).

Determination of polydatin in rats plasma by LC-MS/MS

1.
Department of Pharmaceutics, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
2.
Laboratory of Toxicology & Pharmacology, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China

摘要: 目的建立测定大鼠血浆中虎杖苷浓度的LC-MS/MS方法。方法采用LC-MS/MS测定大鼠血浆中虎杖苷的浓度,以二苯乙烯苷为内标,血浆样品用乙腈沉淀蛋白,色谱柱为Agilent Zorbax SB-C₁₈ (100 mm×2.1 mm, 3.5 μm),流动相为甲醇-乙腈-0.1%甲酸水溶液(18∶15∶67),流速0.3 ml/min,柱温30 ℃。结果虎杖苷的线性范围为1.0~5 000.0 ng/ml (r=0.998 4),最低定量检测浓度为1.0
- 虎杖苷 /
- LC-MS/MS /
- 血药浓度
Abstract: Objective To establish a LC-MS/MS method for determination of polydatin concentration in rats plasma. Methods The plasma concentration of polydatin was determined by LC-MS/MS. Using stilbene glucoside as internal standard, after precipitation of the plasma proteins with methanol, the analytes were separated on an agilent zorbax SB-C₁₈ reversed-phase column with methanol-acetonitrile- 0.1% formic acid (18 : 15 : 67, v/v) and detected by electrospray ionization (ESI) mass spectrometry in negative multiple reaction monitoring (MRM) mode. The flow rate was 0.3 ml/min. Column temperature was maintained at 30 ℃. Results The calibration curves with good linearity (r=0.998 4 for plasma sample) were obtained in the range of 1.0~5000.0 ng/ml for polydatin. The lower limit of quantification (LLOQ) was 1.0 ng/ml. Recoveries were around 78% for the extraction from rats plasma, and good precision and accuracy were achieved. The intra-day and inter-day RSD were both less than 15.0%. This method was feasible for the evaluation of pharmacokinetic profiles of polydatin in rats. Conclusion This method was simple, selective and sensitive. It's suitable for the pharmacokinetic research of polydatin in rats plasma.
- polydatin /
- LC-MS/MS /
- plasma drug concentration

[1]	肖凯,宣利江,徐亚明,等.虎杖的化学成分研究[J].中国药学杂志, 2003,38(1):12.
[2]	高守红,杨少麟,范国荣. 虎杖苷的研究进展[J].药学实践杂志,2005,23(3):145.
[3]	李蓟龙,尹京湘,张力,等. 虎杖中白藜芦醇对豚鼠左心室流出道自律组织电活动的影响[J]. 中国应用生理学杂志,2010,26(3):311,364.
[4]	刘铭,刘华,张国平,等. 虎杖苷对脑出血性损伤大鼠血清白细胞介素-1β和脑组织Bcl-2蛋白表达的影响[J]. 中草药,2010, 41(12):2010.
[5]	Gao S, Fan G, Hong Z, et al. HPLC determination of polydatin in rat biological matrices: application to pharmacokinetic studies[J]. J Pharmaceut Biomed,2006, 41(1): 240.
[6]	吕春艳,张兰桐,袁志芳,等. 虎杖苷在大鼠体内的药动学特点和组织分布研究[J].中草药,2006,38(2):235.
[7]	林建海,李笑宏,唐跃年,等. 白藜芦醇苷的HPLC法分析及其在苗猪体内的药代动力学研究[J]. 药物分析杂志, 2001,21(05):325.
[8]	杨润涛,周四元,章翔,等.液相色谱-串联质谱法同时测定血浆中白藜芦醇苷及其代谢产物[J]. 分析化学,2007,35(9):1309.
[9]	Zhou S, Yang R,Teng Z,et al. Dose-dependent absorption and metabolism of trans-polydatin in rats[J]. J Agr Food Chem, 2009,57(11): 4572.

[1]	崔晓雪, 王迎夏, 周广鑫, 黄孝宇, 王留明, 刘晓. 人参、西洋参和三七的不同部位丙二酰人参皂苷Rb3含量研究 . 药学实践与服务, 2026, 44(): 1-6. doi: 10.12206/j.issn.2097-2024.202410049
[2]	张婷婷, 黄晶晶, 梁静蕾, 庞涛, 陈万生, 张凤. 基于超高效液相质谱联用技术分析不同产地桔梗皂苷类成分的差异 . 药学实践与服务, 2026, 44(4): 189-199, 208. doi: 10.12206/j.issn.2097-2024.202506026
[3]	乔方良, 蒋益萍, 夏天爽, 刘爱军, 赵凯, 辛海量. 对萼猕猴桃苷E提取分离纯化工艺的研究 . 药学实践与服务, 2026, 44(3): 137-143. doi: 10.12206/j.issn.2097-2024.202407001
[4]	李焕, 张圳, 冯家懿, 张卫东, 刘霞. 橄榄苦苷在肌肉耐力提升中的作用研究 . 药学实践与服务, 2026, 44(3): 126-131. doi: 10.12206/j.issn.2097-2024.202510011
[5]	胡文宇, 史文俊, 王学武, 李硕硕. 金线莲苷对小鼠酒精性肝损伤的作用机制研究 . 药学实践与服务, 2026, 44(1): 12-19. doi: 10.12206/j.issn.2097-2024.202309030
[6]	桂明珠, 李静, 李志玲. 儿童伏立康唑的血药浓度与CYP2C19、CYP2C9和CYP3A5基因多态性的相关性研究 . 药学实践与服务, 2026, 44(2): 76-79, 107. doi: 10.12206/j.issn.2097-2024.202402020
[7]	林君容, 张敏新, 赵雪梅, 黄爱文. 中心切割二维液相色谱法测定人血浆中拉莫三嗪血药浓度的研究 . 药学实践与服务, 2026, 44(1): 53-58. doi: 10.12206/j.issn.2097-2024.202306020
[8]	冯一帆, 严啸东, 张文彬, 李炳锋, 郭美丽. 菸花苷长期给药对脑缺血再灌注损伤大鼠神经功能的影响 . 药学实践与服务, 2025, 43(5): 228-234. doi: 10.12206/j.issn.2097-2024.202407038
[9]	江冼芮, 段雅倩, 刘畅, 张成中. 淫羊藿中黄酮苷类化合物的群体感应抑制作用研究 . 药学实践与服务, 2025, 43(4): 169-173. doi: 10.12206/j.issn.2097-2024.202409060
[10]	闻韬, 焦广洋, 赵梦沛, 岳小强, 张凤, 陈万生. 基于UHPLC-Q-TOF/MS结合网络药理学研究柴胡桂枝干姜汤治疗慢性非萎缩性胃炎的物质基础及疗效机制 . 药学实践与服务, 2025, 43(9): 455-462. doi: 10.12206/j.issn.2097-2024.202312061
[11]	晁亮, 王辉, 沈淑琦, 游飘雪, 冀凯宏, 洪战英. 基于UHPLC-Q/TOF-MS代谢组学策略的葛根-知母药对防治阿尔茨海默病的药效与作用机制研究 . 药学实践与服务, 2025, 43(1): 30-40. doi: 10.12206/j.issn.2097-2024.202409035
[12]	刘红霞, 孙延文, 韩菲, 周妍, 孙华君, 丁丽琴. UPLC-MS/MS法测定复方地黄合剂中10种指标性成分的含量 . 药学实践与服务, 2025, 43(8): 390-394. doi: 10.12206/j.issn.2097-2024.202312068
[13]	詹济华, 颜滢. 低蛋白血症对头孢哌酮舒巴坦血药浓度的影响 . 药学实践与服务, 2025, 43(7): 344-347. doi: 10.12206/j.issn.2097-2024.202309040
[14]	景凯, 杨慈荣, 张圳, 臧艺蓓, 刘霞. 黄芪甲苷衍生物治疗慢性心力衰竭小鼠的药效评价及作用机制研究 . 药学实践与服务, 2024, 42(5): 190-197. doi: 10.12206/j.issn.2097-2024.202310004
[15]	陈炳辰, 佟达丰, 万苗, 闫飞虎, 姚建忠. UPLC-MS/MS法测定小鼠血浆中紫杉醇脂肪酸酯前药及其药代动力学研究 . 药学实践与服务, 2024, 42(8): 341-345. doi: 10.12206/j.issn.2097-2024.202404082
[16]	陈方剑, 骆锦前, 王志君, 胡叶帅, 孙煜昕, 宋洪杰. HPLC-MS/MS同时测定感冒安颗粒中5种黄酮成分的含量 . 药学实践与服务, 2024, 42(9): 402-406. doi: 10.12206/j.issn.2097-2024.202403030
[17]	李丹, 戴贤春, 王芳珍, 陈奕含, 杨萍, 刘继勇. HPLC-MS/MS测定当归六黄汤中4种不同成分的含量 . 药学实践与服务, 2024, 42(6): 248-252, 266. doi: 10.12206/j.issn.2097-2024.202305007

点击查看大图

计量

文章访问数: 7092
HTML全文浏览量: 415
PDF下载量: 397
被引次数: 0

LC-MS/MS测定大鼠血浆中虎杖苷浓度

doi: 10.3969/j.issn.1006-0111.2012.01.012

1. 第二军医大学药学院药剂学教研室,上海 200433

2. 第二军医大学海医系毒物药物研究室,上海 200433

基金项目: 上海市科委中药现代化项目(08DZ1971504);国家自然科学基金(20872179,30472141).

收稿日期: 2011-02-10
修回日期: 2011-12-21

关键词:

虎杖苷 /

LC-MS/MS /

血药浓度

摘要: 目的建立测定大鼠血浆中虎杖苷浓度的LC-MS/MS方法。方法采用LC-MS/MS测定大鼠血浆中虎杖苷的浓度,以二苯乙烯苷为内标,血浆样品用乙腈沉淀蛋白,色谱柱为Agilent Zorbax SB-C₁₈ (100 mm×2.1 mm, 3.5 μm),流动相为甲醇-乙腈-0.1%甲酸水溶液(18∶15∶67),流速0.3 ml/min,柱温30 ℃。结果虎杖苷的线性范围为1.0~5 000.0 ng/ml (r=0.998 4),最低定量检测浓度为1.0

English Abstract

Determination of polydatin in rats plasma by LC-MS/MS

1. Department of Pharmaceutics, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

2. Laboratory of Toxicology & Pharmacology, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China

Received Date: 2011-02-10
Rev Recd Date: 2011-12-21

Keywords:

Abstract: Objective To establish a LC-MS/MS method for determination of polydatin concentration in rats plasma. Methods The plasma concentration of polydatin was determined by LC-MS/MS. Using stilbene glucoside as internal standard, after precipitation of the plasma proteins with methanol, the analytes were separated on an agilent zorbax SB-C₁₈ reversed-phase column with methanol-acetonitrile- 0.1% formic acid (18 : 15 : 67, v/v) and detected by electrospray ionization (ESI) mass spectrometry in negative multiple reaction monitoring (MRM) mode. The flow rate was 0.3 ml/min. Column temperature was maintained at 30 ℃. Results The calibration curves with good linearity (r=0.998 4 for plasma sample) were obtained in the range of 1.0~5000.0 ng/ml for polydatin. The lower limit of quantification (LLOQ) was 1.0 ng/ml. Recoveries were around 78% for the extraction from rats plasma, and good precision and accuracy were achieved. The intra-day and inter-day RSD were both less than 15.0%. This method was feasible for the evaluation of pharmacokinetic profiles of polydatin in rats. Conclusion This method was simple, selective and sensitive. It's suitable for the pharmacokinetic research of polydatin in rats plasma.

丁雪鹰, 侯晓丽, 孙铭学, 肖凯. LC-MS/MS测定大鼠血浆中虎杖苷浓度[J]. 药学实践与服务, 2012, 30(1): 45-48. doi: 10.3969/j.issn.1006-0111.2012.01.012

引用本文:

丁雪鹰, 侯晓丽, 孙铭学, 肖凯. LC-MS/MS测定大鼠血浆中虎杖苷浓度[J]. 药学实践与服务, 2012, 30(1): 45-48. doi: 10.3969/j.issn.1006-0111.2012.01.012

Citation: