HPLC法测定藏药螃蟹甲中苯乙醇苷类化合物的含量

李茂星; 张超; 乌兰; 尉丽力; 邱建国; 张泉龙; 贾正平

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HPLC法测定藏药螃蟹甲中苯乙醇苷类化合物的含量

李茂星, 张超, 乌兰, 尉丽力, 邱建国, 张泉龙, 贾正平

文章导航 > 药学实践与服务 > 2011 > 29(5): 379-381,400

李茂星, 张超, 乌兰, 尉丽力, 邱建国, 张泉龙, 贾正平. HPLC法测定藏药螃蟹甲中苯乙醇苷类化合物的含量[J]. 药学实践与服务, 2011, 29(5): 379-381,400.

引用本文:

李茂星, 张超, 乌兰, 尉丽力, 邱建国, 张泉龙, 贾正平. HPLC法测定藏药螃蟹甲中苯乙醇苷类化合物的含量[J]. 药学实践与服务, 2011, 29(5): 379-381,400.

LI Mao-xing, ZHANG Chao, WU Lan, WEI Li-li, QIN Jian-guo, ZHANG Quan-long, JIA Zheng-ping. Determination of phenylethanoid glycosides in Phlomis younghusbandii by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(5): 379-381,400.

Citation:

LI Mao-xing, ZHANG Chao, WU Lan, WEI Li-li, QIN Jian-guo, ZHANG Quan-long, JIA Zheng-ping. Determination of phenylethanoid glycosides in Phlomis younghusbandii by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(5): 379-381,400.

HPLC法测定藏药螃蟹甲中苯乙醇苷类化合物的含量

1.
兰州军区兰州总医院药剂科,全军高原环境损伤防治重点实验室,甘肃兰州 730050;兰州大学生命科学学院,甘肃兰州 730000
2.
西北民族大学化工学院,甘肃兰州730030
3.
兰州军区兰州总医院药剂科,全军高原环境损伤防治重点实验室,甘肃兰州 730050

Determination of phenylethanoid glycosides in Phlomis younghusbandii by HPLC

1.
Department of Pharmacy, Lanzhou General Hospital of PLA, Key laboratory of the prevention and cure for the plateau environment damage, PLA, Lanzhou 730050, China;College of Life Science, Lanzhou University, Lanzhou 730000, China
2.
College of Chemical Engineering, Northwest University for Nationalities, Lanzhou 730030, China
3.
Department of Pharmacy, Lanzhou General Hospital of PLA, Key laboratory of the prevention and cure for the plateau environment damage, PLA, Lanzhou 730050, China

摘要: 目的建立RP-HPLC法同时测定藏药螃蟹甲中3个苯乙醇苷的含量。方法色谱柱为Symmetry C₁₈(4.6 mm ×150 mm,5 μm),流动相为甲醇-水(36:64),冰醋酸调pH至4.5;流速1.0 ml/min;柱温20 ℃,检测波长330 nm。结果毛蕊花糖苷、alyssonoside、异毛蕊花糖苷分别在20~500(r=0.999 6), 10~130(r=0.999 5), 10~130 (r=0.999 7) μg/ml范围间与峰面积线性关系良好;平均加样回收率均在96%~104%之间,RSD均小于5%(n=6)。结论该方法专属性强、灵敏度高、精确度与重现性好,可作为螃蟹甲药材的质量控制方法。
- 螃蟹甲 /
- 苯乙醇苷 /
- 高压液相色谱法
Abstract: Objective To establish a HPLC method for the determination of three phenylethanoid glycosides in Phlomis younghusbandii. Methods HPLC analysis was performed on Symmetry C₁₈ (4.6 mm×150 mm, 5 μm) column and the mixture of methanol-water (36:64, pH=4.5, HAc) was used as the mobile phase with a flow rate of 1.0 ml/min. The column temperature was 20 ℃ and the detection wavelength was 330 nm. Results The linear ranges of acteoside,alyssonoside,isoacteoside were 20~500(r=0.999 6), 10~130(r=0.999 5), 10~130 (r=0.999 7) μg/ml, respectively. The average recoveries (n=6) of them were all between 96% and 104%, RSD<5%. Conclusions This method was special, sensitive, accurate and with good repeatability, which could be used for quality control of Phlomis younghusbandii.
- Phlomis younghusbandii /
- phenylethanoid glycosides /
- HPLC

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HPLC法测定藏药螃蟹甲中苯乙醇苷类化合物的含量

1. 兰州军区兰州总医院药剂科,全军高原环境损伤防治重点实验室,甘肃兰州 730050;兰州大学生命科学学院,甘肃兰州 730000

2. 西北民族大学化工学院,甘肃兰州730030

3. 兰州军区兰州总医院药剂科,全军高原环境损伤防治重点实验室,甘肃兰州 730050

收稿日期: 2011-02-23
修回日期: 2011-04-04

关键词:

螃蟹甲 /

苯乙醇苷 /

高压液相色谱法

摘要: 目的建立RP-HPLC法同时测定藏药螃蟹甲中3个苯乙醇苷的含量。方法色谱柱为Symmetry C₁₈(4.6 mm ×150 mm,5 μm),流动相为甲醇-水(36:64),冰醋酸调pH至4.5;流速1.0 ml/min;柱温20 ℃,检测波长330 nm。结果毛蕊花糖苷、alyssonoside、异毛蕊花糖苷分别在20~500(r=0.999 6), 10~130(r=0.999 5), 10~130 (r=0.999 7) μg/ml范围间与峰面积线性关系良好;平均加样回收率均在96%~104%之间,RSD均小于5%(n=6)。结论该方法专属性强、灵敏度高、精确度与重现性好,可作为螃蟹甲药材的质量控制方法。

English Abstract

Determination of phenylethanoid glycosides in Phlomis younghusbandii by HPLC

1. Department of Pharmacy, Lanzhou General Hospital of PLA, Key laboratory of the prevention and cure for the plateau environment damage, PLA, Lanzhou 730050, China;College of Life Science, Lanzhou University, Lanzhou 730000, China

2. College of Chemical Engineering, Northwest University for Nationalities, Lanzhou 730030, China

3. Department of Pharmacy, Lanzhou General Hospital of PLA, Key laboratory of the prevention and cure for the plateau environment damage, PLA, Lanzhou 730050, China

Received Date: 2011-02-23
Rev Recd Date: 2011-04-04

Keywords:

Abstract: Objective To establish a HPLC method for the determination of three phenylethanoid glycosides in Phlomis younghusbandii. Methods HPLC analysis was performed on Symmetry C₁₈ (4.6 mm×150 mm, 5 μm) column and the mixture of methanol-water (36:64, pH=4.5, HAc) was used as the mobile phase with a flow rate of 1.0 ml/min. The column temperature was 20 ℃ and the detection wavelength was 330 nm. Results The linear ranges of acteoside,alyssonoside,isoacteoside were 20~500(r=0.999 6), 10~130(r=0.999 5), 10~130 (r=0.999 7) μg/ml, respectively. The average recoveries (n=6) of them were all between 96% and 104%, RSD<5%. Conclusions This method was special, sensitive, accurate and with good repeatability, which could be used for quality control of Phlomis younghusbandii.

李茂星, 张超, 乌兰, 尉丽力, 邱建国, 张泉龙, 贾正平. HPLC法测定藏药螃蟹甲中苯乙醇苷类化合物的含量[J]. 药学实践与服务, 2011, 29(5): 379-381,400.

引用本文:

李茂星, 张超, 乌兰, 尉丽力, 邱建国, 张泉龙, 贾正平. HPLC法测定藏药螃蟹甲中苯乙醇苷类化合物的含量[J]. 药学实践与服务, 2011, 29(5): 379-381,400.

Citation: