分泌型磷脂酶A<SUB>2</SUB>在人皮肤黑素瘤细胞和组织中的表达和分布

张立超; 张永佳; 顾伟鹰; 王慧菁; 时扣荣

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分泌型磷脂酶A₂在人皮肤黑素瘤细胞和组织中的表达和分布

张立超, 张永佳, 顾伟鹰, 王慧菁, 时扣荣

文章导航 > 药学实践与服务 > 2009 > 27(6): 417-420

张立超, 张永佳, 顾伟鹰, 王慧菁, 时扣荣. 分泌型磷脂酶A2在人皮肤黑素瘤细胞和组织中的表达和分布[J]. 药学实践与服务, 2009, 27(6): 417-420.

引用本文:

张立超, 张永佳, 顾伟鹰, 王慧菁, 时扣荣. 分泌型磷脂酶A₂在人皮肤黑素瘤细胞和组织中的表达和分布[J]. 药学实践与服务, 2009, 27(6): 417-420.

ZHANG Li-chao, ZHANG Yong-jia, GU Wei-ying, WANG Hui-jing, SHI Kou-rong. Expression and distribution of secretory phospholipase A2 in human skin melanoma cells and tissues[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(6): 417-420.

Citation:

ZHANG Li-chao, ZHANG Yong-jia, GU Wei-ying, WANG Hui-jing, SHI Kou-rong. Expression and distribution of secretory phospholipase A₂ in human skin melanoma cells and tissues[J]. Journal of Pharmaceutical Practice and Service, 2009, 27(6): 417-420.

分泌型磷脂酶A₂在人皮肤黑素瘤细胞和组织中的表达和分布

上海市第七人民医院药剂科,上海200137

基金项目: 国家自然科学基金项目(30472103);浦东新区卫生系统学科带头人项目(PWRd2008-07);浦东新区科委科研项目(PKJ2009Y23)

Expression and distribution of secretory phospholipase A₂ in human skin melanoma cells and tissues

Department of Pharmacy, Shanghai Seventh People′s Hospital, Shanghai 200137, China

摘要: 目的:比较分泌型磷脂酶A₂(sPLA₂)在人恶性黑素瘤A375细胞、黑素瘤组织与健康人皮肤细胞、组织中的表达和分布。方法:分别采用ELISA法和Western Blot法检测细胞上清液和细胞内sPLA₂的表达,免疫组化法考察人皮肤组织中sPLA₂的表达和分布。结果:恶性黑素瘤A375细胞上清液的sPLA₂浓度为(177.27±13.57)pg/mL,明显高于人表皮HaCaT细胞的(21.42±5.05)pg/mL(P<0.05)和真皮成纤维细胞的(2.75±1.23)pg/mL(P<0.05);其细胞内sPLA₂/GAPDH的灰度比值为7.03±1.31,亦明显高于HaCaT细胞的1.45±0.37(P<0.05)和成纤维细胞的0.31±0.11(P<0.05);黑素瘤组织中呈现大量sPLA₂阳性细胞和颗粒,而健康人表皮和真皮组织中则未见该阳性细胞和颗粒。结论:sPLA₂在人恶性黑素瘤A375细胞和黑素瘤组织中较健康皮肤细胞和组织均有较高表达和分布,提示sPLA₂有可能成为皮肤黑素瘤靶向给药的新靶点。
- 分泌型磷脂酶A₂ /
- 黑素瘤 /
- 表达 /
- 分布
Abstract: Objective :To investigate the expression and distribution of secretory phospholipase A₂(sPLA₂) in A375 human malignant melanoma cells and melanoma tissues compared with normal human skin cells and tissues. Methods :The expression of sPLA₂ in the clear supernatant and intra-cellular of cells was detected by ELISA method and Western Blot method,respectively.Immunohistochemical method was used to investigate the expression and distribution of sPLA₂ in the human skin tissues. Results :The levels of sPLA₂ in the clear supernatant of A375 malignant melanoma cells was(177.27±13.57) pg/mL,which was significantly higher(P<0.05) than the group of human epidermal HaCaT cells(21.42±5.05) pg/ml and the group of dermal fibroblast cells(2.75±1.23) pg/mL,respectively.The ratio of sPLA₂/GAPDH in the A375 cells was(7.03±1.31),which was also significantly higher(P<0.05) than the group of human epidermal HaCaT cells(1.45±0.37) and the group of dermal fibroblast cells(0.31±0.11),respectively.Many sPLA₂ positive cells and granules were presented in the tumor tissues.In contrast,positive cells and granules above were unobserved in the normal human epidermal and dermal tissues. Conclusion :sPLA₂ is expressed and distributed at higher levels in A375 human malignant melanoma cells and melanoma tissues compared with the normal skin cells and tissues,indicating the sPLA₂ maybe a novel trigger for targeted drug delivery to skin melanoma.
- secretory phospholipase A₂ /
- melanoma /
- expression /
- distribution

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分泌型磷脂酶A₂在人皮肤黑素瘤细胞和组织中的表达和分布

上海市第七人民医院药剂科,上海200137

基金项目: 国家自然科学基金项目(30472103);浦东新区卫生系统学科带头人项目(PWRd2008-07);浦东新区科委科研项目(PKJ2009Y23)

收稿日期: 2009-10-25

关键词:

分泌型磷脂酶A₂ /

黑素瘤 /

表达 /

分布

摘要: 目的:比较分泌型磷脂酶A₂(sPLA₂)在人恶性黑素瘤A375细胞、黑素瘤组织与健康人皮肤细胞、组织中的表达和分布。方法:分别采用ELISA法和Western Blot法检测细胞上清液和细胞内sPLA₂的表达,免疫组化法考察人皮肤组织中sPLA₂的表达和分布。结果:恶性黑素瘤A375细胞上清液的sPLA₂浓度为(177.27±13.57)pg/mL,明显高于人表皮HaCaT细胞的(21.42±5.05)pg/mL(P<0.05)和真皮成纤维细胞的(2.75±1.23)pg/mL(P<0.05);其细胞内sPLA₂/GAPDH的灰度比值为7.03±1.31,亦明显高于HaCaT细胞的1.45±0.37(P<0.05)和成纤维细胞的0.31±0.11(P<0.05);黑素瘤组织中呈现大量sPLA₂阳性细胞和颗粒,而健康人表皮和真皮组织中则未见该阳性细胞和颗粒。结论:sPLA₂在人恶性黑素瘤A375细胞和黑素瘤组织中较健康皮肤细胞和组织均有较高表达和分布,提示sPLA₂有可能成为皮肤黑素瘤靶向给药的新靶点。

English Abstract

Expression and distribution of secretory phospholipase A₂ in human skin melanoma cells and tissues

Department of Pharmacy, Shanghai Seventh People′s Hospital, Shanghai 200137, China

Received Date: 2009-10-25

Keywords:

Abstract: Objective :To investigate the expression and distribution of secretory phospholipase A₂(sPLA₂) in A375 human malignant melanoma cells and melanoma tissues compared with normal human skin cells and tissues. Methods :The expression of sPLA₂ in the clear supernatant and intra-cellular of cells was detected by ELISA method and Western Blot method,respectively.Immunohistochemical method was used to investigate the expression and distribution of sPLA₂ in the human skin tissues. Results :The levels of sPLA₂ in the clear supernatant of A375 malignant melanoma cells was(177.27±13.57) pg/mL,which was significantly higher(P<0.05) than the group of human epidermal HaCaT cells(21.42±5.05) pg/ml and the group of dermal fibroblast cells(2.75±1.23) pg/mL,respectively.The ratio of sPLA₂/GAPDH in the A375 cells was(7.03±1.31),which was also significantly higher(P<0.05) than the group of human epidermal HaCaT cells(1.45±0.37) and the group of dermal fibroblast cells(0.31±0.11),respectively.Many sPLA₂ positive cells and granules were presented in the tumor tissues.In contrast,positive cells and granules above were unobserved in the normal human epidermal and dermal tissues. Conclusion :sPLA₂ is expressed and distributed at higher levels in A375 human malignant melanoma cells and melanoma tissues compared with the normal skin cells and tissues,indicating the sPLA₂ maybe a novel trigger for targeted drug delivery to skin melanoma.

张立超, 张永佳, 顾伟鹰, 王慧菁, 时扣荣. 分泌型磷脂酶A2在人皮肤黑素瘤细胞和组织中的表达和分布[J]. 药学实践与服务, 2009, 27(6): 417-420.

引用本文:

张立超, 张永佳, 顾伟鹰, 王慧菁, 时扣荣. 分泌型磷脂酶A₂在人皮肤黑素瘤细胞和组织中的表达和分布[J]. 药学实践与服务, 2009, 27(6): 417-420.

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分泌型磷脂酶A2在人皮肤黑素瘤细胞和组织中的表达和分布

Expression and distribution of secretory phospholipase A2 in human skin melanoma cells and tissues

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分泌型磷脂酶A2在人皮肤黑素瘤细胞和组织中的表达和分布

上海市第七人民医院药剂科,上海200137

English Abstract

Expression and distribution of secretory phospholipase A2 in human skin melanoma cells and tissues

Department of Pharmacy, Shanghai Seventh People′s Hospital, Shanghai 200137, China

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分泌型磷脂酶A₂在人皮肤黑素瘤细胞和组织中的表达和分布

Expression and distribution of secretory phospholipase A₂ in human skin melanoma cells and tissues

分泌型磷脂酶A₂在人皮肤黑素瘤细胞和组织中的表达和分布

Expression and distribution of secretory phospholipase A₂ in human skin melanoma cells and tissues