反相高效液相色谱法测定珍黄丸中黄芩苷的含量
Determination of baicalin in Zhenhuang Wan by RP-HPLC
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摘要: 目的 应用高效液相色谱法测定珍黄丸中黄芩苷的含量.方法 色谱柱为Alltima C18(5μm,4.6×150mm),流动相为甲醇-水-冰醋酸(50:50:1),检测波长为273nm,流速为1.0mL/min.结果 黄芩苷在7.4444-37.220μg范围内进样量与峰面积呈良好线性关系(r=0.999 7);平均加样回收率为98.58%,RSD =0.65%(n=5).结论 本方法测定结果准确、灵敏、重现性好.Abstract: Objective To develop a RP-HPLC method for determination of baicalin in Zhenhuang Wan.Methods Alltima C18 column was used and methanol-water-acetic acid glacial (50 : 50 :1) was used as mobile phase,detection wavelegth was 273nm.Results There was a good linear relationship between the concentration of baicalin and absorption area value in range of 7.444 - 37.220μg r = 0.999 7.The average recovery was 98.55% with RSD=0.65% (n=5).Conclusion The method was repeatable and accurate.
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Key words:
- RP-HPLC /
- Zhen huang Wan /
- baicalin
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