大苞雪莲抗缺氧有效成分对模拟高原缺氧小鼠物质代谢的影响

马慧萍; 武柠子; 王昕; 景临林; 贾正平; 张俊

doi:10.3969/j.issn.1006-0111.2018.03.011

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

x 关闭永久关闭

重要提示

《药学实践与服务》杂志不接收生物战剂、毒剂相关内容的稿件。对于涉军信息类论文如需投稿，请务必投稿前电话或邮箱咨询。联系电话：021-81871323，邮箱：yxsjzzs@163.com。敬请谅解，谢谢配合！

《药学实践与服务》编辑部

免责声明

《药学实践与服务》杂志目前不收取审稿费、版面费、加急费等费用，如收到邮件声称是编辑部X编辑，要求加作者微信的，请谨防财产损失！编辑部用于作者校稿时微信绑定的邮件是通过yxsjzzs@163.com发送的，标题是《药学实践与服务》XML数字出版服务微信绑定，请区分开！

大苞雪莲抗缺氧有效成分对模拟高原缺氧小鼠物质代谢的影响

马慧萍, 武柠子, 王昕, 景临林, 贾正平, 张俊

文章导航 > 药学实践与服务 > 2018 > 36(3): 243-246

马慧萍, 武柠子, 王昕, 景临林, 贾正平, 张俊. 大苞雪莲抗缺氧有效成分对模拟高原缺氧小鼠物质代谢的影响[J]. 药学实践与服务, 2018, 36(3): 243-246. doi: 10.3969/j.issn.1006-0111.2018.03.011

引用本文:

MA Huiping, WU Ningzi, WANG Xin, JING Linlin, JIA Zhengping, ZHANG Jun. Effects of the active constituents from Saussurea involucrata on metabolism of mice at simulated high altitude[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(3): 243-246. doi: 10.3969/j.issn.1006-0111.2018.03.011

Citation:

MA Huiping, WU Ningzi, WANG Xin, JING Linlin, JIA Zhengping, ZHANG Jun. Effects of the active constituents from Saussurea involucrata on metabolism of mice at simulated high altitude[J]. Journal of Pharmaceutical Practice and Service, 2018, 36(3): 243-246. doi: 10.3969/j.issn.1006-0111.2018.03.011

大苞雪莲抗缺氧有效成分对模拟高原缺氧小鼠物质代谢的影响

doi: 10.3969/j.issn.1006-0111.2018.03.011

兰州总医院全军高原环境损伤防治重点实验室, 甘肃兰州 730050

基金项目: 国家自然科学基金项目（81571847，81402848）；甘肃省自然科学基金（145RJZA089）；军队医药卫生科研项目（CLZ14JA01）

Effects of the active constituents from Saussurea involucrata on metabolism of mice at simulated high altitude

Lanzhou General Hospital Key Lab of PLA for Prevention and Treatment of Injuries induced by High Altitude, Lanzhou 730050, China

摘要: 目的研究大苞雪莲（又名新疆雪莲、天山雪莲，Saussurea involucrata）抗缺氧有效成分（石油醚活性部位及其主要单体成分二十八烷）对模拟高原缺氧小鼠水、糖、脂肪、蛋白质等物质代谢的影响。方法将雄性BALB/c小鼠随机分为正常对照组、缺氧模型组、乙酰唑胺阳性药对照组、大苞雪莲石油醚部位组和二十八烷组，单次尾静脉注射给药，给药20 min后，将各组小鼠分别放入低压氧舱中，模拟海拔6 000 m低压低氧环境，维持8 h，检测器官含水量、脏器指数、糖代谢、蛋白质代谢和脂肪代谢等指标变化情况。结果给小鼠预先尾静脉注射石油醚部位200 mg/kg和二十八烷100 mg/kg，可以通过降低脏器中含水量抑制急性高原缺氧引起的心、脑、肺组织水肿（P<0.05或P<0.01），抑制血糖、肌糖原、三酰甘油（TG）、总胆固醇（TC）水平的升高（P<0.05或P<0.01），阻止肝糖原、心肌和脑组织蛋白含量的降低（P<0.05或P<0.01）。结论大苞雪莲石油醚部位和二十八烷能有效调节缺氧小鼠的物质代谢，降低基础代谢水平，保证机体生存所需能量。
- 大苞雪莲乙醇提物 /
- 抗缺氧活性 /
- 物质代谢 /
- 石油醚部位 /
- 二十八烷
Abstract: Objective To investigate the anti-hypoxia effects of octacosane and the petroleum ether extract from Saussurea Involucrate (PESI) on the water, sugar, lipid and protein metabolism of mice at simulated high altitude. Methods The healthy adult male BALB/C mice were randomly divided into normal control group, hypoxic model group, acetazolamide group, the petroleum ether of Saussurea involucrata group and octacosane group. Drugs were administered i.v 20 mins before the mice were exposed to a simulated high altitude of 6 000 m for 8 hours in an animal decompression chamber. The mice were sacrificed at the end of 8 hours. Organ water content, organ indexes and metabolism indicators of sugar, protein and lipid were determined. Results The edema of heart, brain and lung was reduced notably (P<0.05, P<0.01) in the mice received PESI at 200 mg/kg and octacosane at 100 mg/kg. In the treated groups, the increase of blood sugar, muscle glycogen, TG (triglyceride), TC (total cholesterol) were all significantly inhibited, the decrease of liver glycogen, the protein content of heart and brain was also remarkably blocked (P<0.05, P<0.01). Conclusion PESI and octacosane effectively regulate the metabolism of hypoxic mice and reserve the body's energy for survival by lowering the basic metabolism.
- Saussurea involucrata /
- anti-hypoxia activity /
- metabolism /
- petroleum ether extract /
- octacosane

[1]	Imray C,Wright A,Subudhi A,et al.Acute mountain sickness:pathophysiology, prevention, and treatment[J]. Progr Cardiovascul Dis, 2010, 52(6):467-484.
[2]	刘晋,郭长江,吴健全,等. 联合补充维生素B₁、B₂、PP对急性低氧暴露小鼠物质代谢的改善作用[J]. 中国应用生理学杂志,2011,27(2):215-218.
[3]	Ma HP,Fan PC,Jing LL,et al. Anti-hypoxic activity at simulated high altitude was isolated in petroleum ether extract of Saussurea involucrata[J]. J Ethnopharmacol, 2011, 137(3):1510-1515.
[4]	景临林,何蕾,樊鹏程,等. 天山雪莲抗缺氧活性成分研究[J]. 中药材,2015,38(1):89-92.
[5]	Min L, Lan H, Kaixin T, et al. Relationship between autonomic nervous system function and acute mountain sickness[J]. J Med Colleg PLA, 2008, 23(5):276-282.
[6]	王镜岩,朱圣庚,徐长法. 生物化学(下册)[M]. 北京:高等教育出版社,2002,232-247.
[7]	马慧萍,高荣敏,吴金华,等.大苞雪莲乙醇提取物对模拟高原缺氧小鼠物质代谢的影响[J]. 解放军药学学报,2013,29(4):301-304.
[8]	张汝学,贾正平,李茂星,等. 锁阳水提物Part Ⅲ对缺氧小鼠心、脑蛋白含量及病理形态的影响[J]. 西北国防医学杂志,2008,29(4):241-243.
[9]	涂宏海,张汝学,贾正平,等. 三康胶囊对缺氧小鼠糖代谢和HPA轴功能的影响[J]. 解放军药学学报,2009,25(3):216-220.

[1]	吴若南, 汤文敏, 高林, 吴岳林, 罗川, 缪震元. RRx-001衍生物的合成和抗肿瘤活性研究 . 药学实践与服务, 2025, 43(8): 400-403. doi: 10.12206/j.issn.2097-2024.202408053
[2]	邵明聪, 陈虎博, 张懿丹, 李梓嫣, 王丽娜. 乌头煎剂对小鼠RAW264.7巨噬细胞活性和极化的影响 . 药学实践与服务, 2025, 43(7): 329-334. doi: 10.12206/j.issn.2097-2024.202505043
[3]	高兴, 赵凤平, 王文涛, 田巍, 郑灿辉, 陈新. 胡椒的化学成分及其生物活性研究 . 药学实践与服务, 2025, 43(7): 313-319. doi: 10.12206/j.issn.2097-2024.202308065
[4]	卢熠菲, 宋佳, 沈红霞, 赵庆杰. 新型苦参碱衍生物的设计合成与体外抗炎活性研究 . 药学实践与服务, 2025, 43(): 1-8. doi: 10.12206/j.issn.2097-2024.202503005
[5]	郭灵怡, 刘艳超, 高路, 刘瑞瑶, 吕权真, 俞媛. 醋酸卡泊芬净单硬脂酸甘油酯纳米粒抗白色念珠菌感染的增效作用研究 . 药学实践与服务, 2025, 43(3): 136-142, 150. doi: 10.12206/j.issn.2097-2024.202310043
[6]	张霖峰, 孙宇恒, 王冬尧, 李丹, 曹岩, 吕狄亚. 茵陈蒿汤体内成分分析及其抗肝癌作用机制的网络药理学研究 . 药学实践与服务, 2025, 43(): 1-9. doi: 10.12206/j.issn.2097-2024.202501017
[7]	陶宫佳, 陈林林, 宋泽成, 刘梦肖, 王彦. 苦参碱及衍生物的抗炎作用及其机制研究进展 . 药学实践与服务, 2025, 43(4): 163-168, 194. doi: 10.12206/j.issn.2097-2024.202406035
[8]	宋泽成, 马闪闪, 胡巧灵, 仲华, 王彦. 小檗碱与氟康唑合用抗氟康唑耐受白念珠菌的研究 . 药学实践与服务, 2025, 43(2): 87-91. doi: 10.12206/j.issn.2097-2024.202409047
[9]	闻韬, 焦广洋, 赵梦沛, 岳小强, 张凤, 陈万生. 基于UHPLC-Q-TOF/MS结合网络药理学研究柴胡桂枝干姜汤治疗慢性非萎缩性胃炎的物质基础及疗效机制 . 药学实践与服务, 2025, 43(9): 455-462. doi: 10.12206/j.issn.2097-2024.202312061
[10]	晁亮, 王辉, 沈淑琦, 游飘雪, 冀凯宏, 洪战英. 基于UHPLC-Q/TOF-MS代谢组学策略的葛根-知母药对防治阿尔茨海默病的药效与作用机制研究 . 药学实践与服务, 2025, 43(1): 30-40. doi: 10.12206/j.issn.2097-2024.202409035
[11]	关梦瑶, 夏天爽, 何旭辉, 史策, 蒋益萍, 辛海量. 黑蒜多糖抗便秘作用研究 . 药学实践与服务, 2025, 43(4): 190-194. doi: 10.12206/j.issn.2097-2024.202403059
[12]	彭莹, 刘欣, 聂依文, 王歆荷, 年华, 朱建勇. 三种狼毒乙醇提取物对咪喹莫特诱导的银屑病小鼠防治作用研究 . 药学实践与服务, 2025, 43(): 1-6. doi: 10.12206/j.issn.2097-2024.202406029
[13]	邱彦, 吴浩, 董雅芬, 陈业, 王建, 金辉. 新型二氢卟吩化合物的光动力性能及其抗肺癌作用研究 . 药学实践与服务, 2025, 43(): 1-7. doi: 10.12206/j.issn.2097-2024.202304011
[14]	吴若南, 叶爽, 李墨晨轩, 缪震元, 罗川. 冬凌草甲素磺酰脲衍生物的设计与抗炎活性研究 . 药学实践与服务, 2025, 43(7): 335-338, 352. doi: 10.12206/j.issn.2097-2024.202401048
[15]	关欣怡, 杨宇洁, 闻俊. 不同场景下芬太尼类新精神活性物质检测研究进展 . 药学实践与服务, 2025, 43(11): 533-539. doi: 10.12206/j.issn.2097-2024.202308033
[16]	赖立勇, 夏天爽, 徐圣焱, 蒋益萍, 岳小强, 辛海量. 中药青蒿抗氧化活性的谱效关系研究 . 药学实践与服务, 2024, 42(5): 203-210, 216. doi: 10.12206/j.issn.2097-2024.202211012
[17]	毛智毅, 王筱燕, 陈晓颖, 汤逸斐. 度拉糖肽联合二甲双胍对肥胖型2型糖尿病患者机体代谢、体脂成分及血清脂肪因子的影响 . 药学实践与服务, 2024, 42(7): 305-309. doi: 10.12206/j.issn.2097-2024.202305032
[18]	孙丹倪, 黄勇, 张嘉宝, 王培. 代谢相关脂肪性肝病的无创诊断与药物治疗 . 药学实践与服务, 2024, 42(10): 411-418. doi: 10.12206/j.issn.2097-2024.202403049
[19]	白学鑫, 陈玉平, 盛春泉, 武善超. 具核梭杆菌小分子抑制剂的筛选及其抗结直肠癌活性研究 . 药学实践与服务, 2024, 42(12): 503-507. doi: 10.12206/j.issn.2097-2024.202405009
[20]	冯志惠, 邓仪卿, 叶冰, 安培, 张宏, 张海军. 雀梅藤石油醚提取物诱导三阴性乳腺癌细胞凋亡的实验研究 . 药学实践与服务, 2024, 42(6): 253-259. doi: 10.12206/j.issn.2097-2024.202311055

点击查看大图

计量

文章访问数: 4122
HTML全文浏览量: 469
PDF下载量: 498
被引次数: 0

大苞雪莲抗缺氧有效成分对模拟高原缺氧小鼠物质代谢的影响

doi: 10.3969/j.issn.1006-0111.2018.03.011

兰州总医院全军高原环境损伤防治重点实验室, 甘肃兰州 730050

基金项目: 国家自然科学基金项目（81571847，81402848）；甘肃省自然科学基金（145RJZA089）；军队医药卫生科研项目（CLZ14JA01）

收稿日期: 2017-11-08
修回日期: 2018-01-08

关键词:

摘要: 目的研究大苞雪莲（又名新疆雪莲、天山雪莲，Saussurea involucrata）抗缺氧有效成分（石油醚活性部位及其主要单体成分二十八烷）对模拟高原缺氧小鼠水、糖、脂肪、蛋白质等物质代谢的影响。方法将雄性BALB/c小鼠随机分为正常对照组、缺氧模型组、乙酰唑胺阳性药对照组、大苞雪莲石油醚部位组和二十八烷组，单次尾静脉注射给药，给药20 min后，将各组小鼠分别放入低压氧舱中，模拟海拔6 000 m低压低氧环境，维持8 h，检测器官含水量、脏器指数、糖代谢、蛋白质代谢和脂肪代谢等指标变化情况。结果给小鼠预先尾静脉注射石油醚部位200 mg/kg和二十八烷100 mg/kg，可以通过降低脏器中含水量抑制急性高原缺氧引起的心、脑、肺组织水肿（P<0.05或P<0.01），抑制血糖、肌糖原、三酰甘油（TG）、总胆固醇（TC）水平的升高（P<0.05或P<0.01），阻止肝糖原、心肌和脑组织蛋白含量的降低（P<0.05或P<0.01）。结论大苞雪莲石油醚部位和二十八烷能有效调节缺氧小鼠的物质代谢，降低基础代谢水平，保证机体生存所需能量。

English Abstract

Effects of the active constituents from Saussurea involucrata on metabolism of mice at simulated high altitude

Lanzhou General Hospital Key Lab of PLA for Prevention and Treatment of Injuries induced by High Altitude, Lanzhou 730050, China

Received Date: 2017-11-08
Rev Recd Date: 2018-01-08

Keywords:

Abstract: Objective To investigate the anti-hypoxia effects of octacosane and the petroleum ether extract from Saussurea Involucrate (PESI) on the water, sugar, lipid and protein metabolism of mice at simulated high altitude. Methods The healthy adult male BALB/C mice were randomly divided into normal control group, hypoxic model group, acetazolamide group, the petroleum ether of Saussurea involucrata group and octacosane group. Drugs were administered i.v 20 mins before the mice were exposed to a simulated high altitude of 6 000 m for 8 hours in an animal decompression chamber. The mice were sacrificed at the end of 8 hours. Organ water content, organ indexes and metabolism indicators of sugar, protein and lipid were determined. Results The edema of heart, brain and lung was reduced notably (P<0.05, P<0.01) in the mice received PESI at 200 mg/kg and octacosane at 100 mg/kg. In the treated groups, the increase of blood sugar, muscle glycogen, TG (triglyceride), TC (total cholesterol) were all significantly inhibited, the decrease of liver glycogen, the protein content of heart and brain was also remarkably blocked (P<0.05, P<0.01). Conclusion PESI and octacosane effectively regulate the metabolism of hypoxic mice and reserve the body's energy for survival by lowering the basic metabolism.