雌激素及三苯氧胺对MCF-7乳腺癌细胞BP1基因表达的影响

王洋; 郑唯强

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名

邮箱

手机号码

标题

留言内容

验证码

雌激素及三苯氧胺对MCF-7乳腺癌细胞BP1基因表达的影响

王洋,
郑唯强

第二军医大学长海医院病理科, 上海, 200433

Effects of estrogen and tamoxifen on the expression of BP1 in MCF-7, a human breast carcinoma cell line

Department of Pathology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China

摘要: 目的检测乳腺癌MCF-7细胞在雌激素(E2)和三苯氧胺(TAM)作用后BPI基因的表达情况.方法用雌激素(E2)和三苯氧胺(TAM)处理培养的处于对数生长期的MCF-7细胞,应用MTT比色分析法筛选最佳作用浓度.以筛选的浓度同步分组作用于MCF-7细胞72h,同时设对照.用原位杂交法检测BP1基因在ER处于激活和抑制状态下的表达状况,用免疫组化检测bcl-2的表达,用TUNEL法检测细胞凋亡状况.分析MCF-7在ER处于不同表达状态下BP1、bcl-2的表达变化和细胞凋亡变化情况.结果 E2在10^-8mol/L、TAM在10^-6mol/L的浓度时,刺激和抑制MCF-7细胞生长的作用最显著.在E2和TAM处理后,MCF-7细胞BP1的表达率分别为95.0%±3.94%、58.5%±5.27%,bcl-2的表达率为80.9%±1.73%、37.8%±2.39%,凋亡指数分别为0.67±0.27、6.7±0.76.分别较对照组76.1%±6.54%、63.5%±3.28%、3.2±0.79之间的差异有统计学意义(P<0.05).结论在ER阳性的乳腺癌细胞,BP1的表达受到ER传导通路的调节,E2可能通过调节BP1的表达水平而在乳腺癌的发生中起作用.BP1可能在调节细胞凋亡的过程中起到抑制作用.BP1阳性表达可能成为预后不良的标志.
- MCF-7细胞 /
- BP1 /
- 细胞凋亡 /
- 原位杂交 /
- bcl-2 /
- 免疫组化
Abstract: Objective To investigate the espression of BP1 gene after estrogen and tamoxifen effected on breast cancer cell line MCF-7 respectively.Methods; With screened concentration of estrogen (10^-8 mol/L) and tamoxifen (10^-6 mol/L) by MTT assay treated with MCF-7 72 hours,then detected the expressions of BP1 mRNA by in situ hybridization method and bcl-2 protein by immuno-histoc-hemistry.Determined quantitatively apoptosis cells of MCF-7 by TUNEL method.Results The percents of BP1 mRNA espression of MCF-7 cells in E2 treated group and TAM treated group were (95.0%±3.94% ) and (58.5%±5.21% ), and those of bcl-2 protein were (80.9%±1.73%) and (37.8%±2.39%), howere, the expressions of BP1 and bcl-2 were (76.1%±6.54%) and (63.5%±3.28%) in control group respectively.There were all significant difference between each two groups (P<0.05 ).There was a significant difference in apoptosis index in either group treated with E2 (0.67±0.27) or TAM (6.7±0.76) in comparing with that of the control group (3.2±0.79) (P<0.05).Conclusion The expression of BP1 mRNA was possibly regulated by ER mediated signal transduction pathway in ER positive breast carcinoma cell line, and BP1 might play an important role in tumorgenesis of breast carcinoma through up-regulate espression mediated by E2 and apoptosis inhibition.BP1 might serve as a poor prognostic markers of breast carcinoma.
- MCF-7 cell /
- BP1 gene /
- apoptosis /
- in situ hybridization /
- bcl-2 /
- Immunohistochemistry

[1]	石晓萍, 吕迁洲, 李晓宇, 许青. 泊沙康唑对比伏立康唑经验治疗或诊断驱动治疗免疫功能低下患者侵袭性霉菌病的成本-效果分析 . 药学实践与服务, 2024, 42(12): 512-519. doi: 10.12206/j.issn.2097-2024.202401050
[2]	唐淑慧, 凤美娟, 薛智霞, 鲁桂华. 帕博利珠单抗治疗所致免疫相关不良反应与中医体质的相关性研究 . 药学实践与服务, 2024, 42(5): 217-222. doi: 10.12206/j.issn.2097-2024.202311029
[3]	晁亮, 王辉, 沈淑琦, 游飘雪, 冀凯宏, 洪战英. 基于UHPLC-Q/TOF-MS代谢组学策略的葛根-知母药对防治阿尔茨海默病的药效与作用机制研究 . 药学实践与服务, 2024, 42(): 1-11. doi: 10.12206/j.issn.2097-2024.202409035
[4]	顾佳钰, 胡馨儿, 王晓飞, 张颖, 张海, 曹岩. 侧流免疫层析定量检测方法的研究进展 . 药学实践与服务, 2024, 42(7): 273-277, 284. doi: 10.12206/j.issn.2097-2024.202307037
[5]	马兹芬, 许维恒, 金煜翔, 薛磊. 食管癌的靶向治疗与免疫治疗研究进展 . 药学实践与服务, 2024, 42(6): 231-237. doi: 10.12206/j.issn.2097-2024.202306008
[6]	毛智毅, 王筱燕, 陈晓颖, 汤逸斐. 度拉糖肽联合二甲双胍对肥胖型2型糖尿病患者机体代谢、体脂成分及血清脂肪因子的影响 . 药学实践与服务, 2024, 42(7): 305-309. doi: 10.12206/j.issn.2097-2024.202305032
[7]	段禹, 刘爱军. 活血化瘀法治疗血管性痴呆的研究进展 . 药学实践与服务, 2024, 42(): 1-6. doi: 10.12206/j.issn.2097-2024.202408045
[8]	邹思, 吴岩斌, 吴锦忠, 吴建国, 黄家兴. 虎奶菇菌核多糖功能化纳米硒抗疲劳功效研究 . 药学实践与服务, 2024, 42(10): 426-432. doi: 10.12206/j.issn.2097-2024.202206072
[9]	岳春华, 贲永光, 王海桥. 基于NLRP1炎症小体探讨百合知母汤抗抑郁的作用机制 . 药学实践与服务, 2024, 42(8): 325-333. doi: 10.12206/j.issn.2097-2024.202401033
[10]	李想, 陆鸿远, 张明玉, 高欢, 姚东, 许子华. 米格列醇激活UCP1介导棕色脂肪对冷暴露小鼠损伤的研究 . 药学实践与服务, 2024, 42(): 1-6. doi: 10.12206/j.issn.2097-2024.202404005
[11]	桂明珠, 李静, 李志玲. 儿童伏立康唑的血药浓度与CYP2C19、CYP2C9和CYP3A5基因多态性的相关性研究 . 药学实践与服务, 2024, 42(): 1-5. doi: 10.12206/j.issn.2097-2024.202402020
[12]	刘丽艳, 余小翠, 孙传铎. 纳武利尤单抗治疗非小细胞肺癌有效性及安全性的Meta分析 . 药学实践与服务, 2024, 42(10): 451-456. doi: 10.12206/j.issn.2097-2024.202310044
[13]	迟文雅, 袁艳, 李伟林, 吴茼妤, 俞媛. 负载骨髓间充质干细胞/白藜芦醇脂质体的水凝胶支架用于创伤性脑损伤治疗 . 药学实践与服务, 2024, 42(): 1-8. doi: 10.12206/j.issn.2097-2024.202406034
[14]	宋雨桐, 夏德润, 顾珩, 唐少文, 易洪刚, 沃红梅. 帕博利珠单抗与铂类化疗方案在晚期非小细胞肺癌一线治疗中的药物经济学评价 . 药学实践与服务, 2024, 42(8): 334-340. doi: 10.12206/j.issn.2097-2024.202303023
[15]	修建平, 杨朝爱, 刘禧澳, 潘乾禹, 韦广旭, 王卫星. 全反式维甲酸对肝星状细胞活化及氧化应激的作用和机制探索 . 药学实践与服务, 2024, 42(7): 291-296. doi: 10.12206/j.issn.2097-2024.202312054
[16]	姜涛, 徐卫凡, 蒋益萍, 夏天爽, 辛海量. 巴戟天丸组方对Aβ损伤成骨细胞的作用及基于网络药理学的机制研究 . 药学实践与服务, 2024, 42(7): 285-290, 296. doi: 10.12206/j.issn.2097-2024.202305011
[17]	杨媛媛, 安晓强, 许佳捷, 江键, 梁媛媛. 正极性驻极体联合5-氟尿嘧啶对瘢痕成纤维细胞生长抑制的协同作用 . 药学实践与服务, 2024, 42(6): 244-247. doi: 10.12206/j.issn.2097-2024.202310027
[18]	冯志惠, 邓仪卿, 叶冰, 安培, 张宏, 张海军. 雀梅藤石油醚提取物诱导三阴性乳腺癌细胞凋亡的实验研究 . 药学实践与服务, 2024, 42(6): 253-259. doi: 10.12206/j.issn.2097-2024.202311055

点击查看大图

计量

文章访问数: 2012
HTML全文浏览量: 186
PDF下载量: 448
被引次数: 0

雌激素及三苯氧胺对MCF-7乳腺癌细胞BP1基因表达的影响

第二军医大学长海医院病理科, 上海, 200433

收稿日期: 2005-05-08

关键词:

MCF-7细胞 /

BP1 /

细胞凋亡 /

原位杂交 /

bcl-2 /

免疫组化

摘要: 目的检测乳腺癌MCF-7细胞在雌激素(E2)和三苯氧胺(TAM)作用后BPI基因的表达情况.方法用雌激素(E2)和三苯氧胺(TAM)处理培养的处于对数生长期的MCF-7细胞,应用MTT比色分析法筛选最佳作用浓度.以筛选的浓度同步分组作用于MCF-7细胞72h,同时设对照.用原位杂交法检测BP1基因在ER处于激活和抑制状态下的表达状况,用免疫组化检测bcl-2的表达,用TUNEL法检测细胞凋亡状况.分析MCF-7在ER处于不同表达状态下BP1、bcl-2的表达变化和细胞凋亡变化情况.结果 E2在10^-8mol/L、TAM在10^-6mol/L的浓度时,刺激和抑制MCF-7细胞生长的作用最显著.在E2和TAM处理后,MCF-7细胞BP1的表达率分别为95.0%±3.94%、58.5%±5.27%,bcl-2的表达率为80.9%±1.73%、37.8%±2.39%,凋亡指数分别为0.67±0.27、6.7±0.76.分别较对照组76.1%±6.54%、63.5%±3.28%、3.2±0.79之间的差异有统计学意义(P<0.05).结论在ER阳性的乳腺癌细胞,BP1的表达受到ER传导通路的调节,E2可能通过调节BP1的表达水平而在乳腺癌的发生中起作用.BP1可能在调节细胞凋亡的过程中起到抑制作用.BP1阳性表达可能成为预后不良的标志.

Effects of estrogen and tamoxifen on the expression of BP1 in MCF-7, a human breast carcinoma cell line

Department of Pathology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China

Received Date: 2005-05-08

Keywords:

Abstract: Objective To investigate the espression of BP1 gene after estrogen and tamoxifen effected on breast cancer cell line MCF-7 respectively.Methods; With screened concentration of estrogen (10^-8 mol/L) and tamoxifen (10^-6 mol/L) by MTT assay treated with MCF-7 72 hours,then detected the expressions of BP1 mRNA by in situ hybridization method and bcl-2 protein by immuno-histoc-hemistry.Determined quantitatively apoptosis cells of MCF-7 by TUNEL method.Results The percents of BP1 mRNA espression of MCF-7 cells in E2 treated group and TAM treated group were (95.0%±3.94% ) and (58.5%±5.21% ), and those of bcl-2 protein were (80.9%±1.73%) and (37.8%±2.39%), howere, the expressions of BP1 and bcl-2 were (76.1%±6.54%) and (63.5%±3.28%) in control group respectively.There were all significant difference between each two groups (P<0.05 ).There was a significant difference in apoptosis index in either group treated with E2 (0.67±0.27) or TAM (6.7±0.76) in comparing with that of the control group (3.2±0.79) (P<0.05).Conclusion The expression of BP1 mRNA was possibly regulated by ER mediated signal transduction pathway in ER positive breast carcinoma cell line, and BP1 might play an important role in tumorgenesis of breast carcinoma through up-regulate espression mediated by E2 and apoptosis inhibition.BP1 might serve as a poor prognostic markers of breast carcinoma.

留言板

雌激素及三苯氧胺对MCF-7乳腺癌细胞BP1基因表达的影响

Effects of estrogen and tamoxifen on the expression of BP1 in MCF-7, a human breast carcinoma cell line

计量

雌激素及三苯氧胺对MCF-7乳腺癌细胞BP1基因表达的影响

第二军医大学长海医院病理科, 上海, 200433

English Abstract

Effects of estrogen and tamoxifen on the expression of BP1 in MCF-7, a human breast carcinoma cell line

Department of Pathology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China

全文HTML

目录

留言板

雌激素及三苯氧胺对MCF-7乳腺癌细胞BP1基因表达的影响

Effects of estrogen and tamoxifen on the expression of BP1 in MCF-7, a human breast carcinoma cell line

计量

出版历程

雌激素及三苯氧胺对MCF-7乳腺癌细胞BP1基因表达的影响

第二军医大学长海医院病理科, 上海, 200433

English Abstract

Effects of estrogen and tamoxifen on the expression of BP1 in MCF-7, a human breast carcinoma cell line

Department of Pathology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China

全文HTML

目录