大孔吸附树脂分离纯化栀子豉汤有效部位的工艺研究

韩燕; 闻俊; 周婷婷; 范国荣

doi:10.3969/j.issn.1006-0111.2015.03.012

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大孔吸附树脂分离纯化栀子豉汤有效部位的工艺研究

韩燕, 闻俊, 周婷婷, 范国荣

文章导航 > 药学实践与服务 > 2015 > 33(3): 238-241

韩燕, 闻俊, 周婷婷, 范国荣. 大孔吸附树脂分离纯化栀子豉汤有效部位的工艺研究[J]. 药学实践与服务, 2015, 33(3): 238-241. doi: 10.3969/j.issn.1006-0111.2015.03.012

引用本文:

韩燕, 闻俊, 周婷婷, 范国荣. 大孔吸附树脂分离纯化栀子豉汤有效部位的工艺研究[J]. 药学实践与服务, 2015, 33(3): 238-241. doi: 10.3969/j.issn.1006-0111.2015.03.012

HAN Yan, WEN Jun, ZHOU Tingting, FAN Guorong. The research of separation and purification technology for effective parts from Zhizichi decoction by macroporous absorption resina[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(3): 238-241. doi: 10.3969/j.issn.1006-0111.2015.03.012

Citation:

HAN Yan, WEN Jun, ZHOU Tingting, FAN Guorong. The research of separation and purification technology for effective parts from Zhizichi decoction by macroporous absorption resina[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(3): 238-241. doi: 10.3969/j.issn.1006-0111.2015.03.012

大孔吸附树脂分离纯化栀子豉汤有效部位的工艺研究

doi: 10.3969/j.issn.1006-0111.2015.03.012

1.
福建中医药大学药学院, 福建福州 350108
2.
第二军医大学药学院药物分析学教研室, 上海 200433
3.
福建中医药大学药学院, 福建福州 350108;第二军医大学药学院药物分析学教研室, 上海 200433

基金项目: 国家自然科学基金资助项目(30801541)

The research of separation and purification technology for effective parts from Zhizichi decoction by macroporous absorption resina

1.
College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China
2.
School of Pharmacy, The Second Military Medical University, Shanghai 200433, China
3.
College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China;School of Pharmacy, The Second Military Medical University, Shanghai 200433, China

摘要: 目的研究大孔树脂分离纯化栀子豉汤有效成分的工艺条件及优化。方法采用紫外分光光度法测定总环烯醚萜苷和总大豆异黄酮的量,以总环烯醚萜苷和总大豆异黄酮的含量作为指标,采用单因素实验考察上样液浓度、上样量、洗脱剂浓度及用量等对分离纯化工艺的影响。结果最佳工艺条件:上样液最佳吸附浓度为0.1 g/ml(该浓度以每1 ml中所含生药材质量计),上样量与树脂体积比是2:1,吸附时间2 h,依次用1 BV(柱体积)水,6 BV 20%乙醇洗脱,6 BV 60%乙醇洗脱,收集洗脱液。结论该纯化工艺合理、稳定,可推广于工业生产。
- 栀子豉汤 /
- 大孔吸附树脂 /
- 分离纯化 /
- 总环烯醚萜苷 /
- 总大豆异黄酮
Abstract: Objective To optimize separation and purification technology of Zhizichi decoction by macroporous resina. Methods The content of total iridoid glycosides and total isoflavones were determined by ultraviolet spectrophotometry. Taking the content of total iridoid glycosides and total isoflavones as indexes, the effect of the concentration of sample solution, medicinal herbs on the amount of sample, concentration and volume of eluent was investigated by single factor test. Results The optimum separation and purification technology was as following: the concentration of sample solution was 0.1 g/ml, the volume ratio of resin to material drug was 2:1, the adsorption time was 2 h, and the sample was firstly eluted with 1 BV water, then 6 BV 20% ethanol and 60% ethanol, and the eluent was collected. Conclusion This optimized separation and purification technology was reasonable and stable, and it could be extended to large-scale production applications.
- Zhizichi decoction /
- macroporous absorption resin /
- separation and purification /
- total iridoid glycosides /
- total isoflavones

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大孔吸附树脂分离纯化栀子豉汤有效部位的工艺研究

doi: 10.3969/j.issn.1006-0111.2015.03.012

1. 福建中医药大学药学院, 福建福州 350108

2. 第二军医大学药学院药物分析学教研室, 上海 200433

3. 福建中医药大学药学院, 福建福州 350108;第二军医大学药学院药物分析学教研室, 上海 200433

基金项目: 国家自然科学基金资助项目(30801541)

收稿日期: 2014-12-18
修回日期: 2015-01-28

关键词:

摘要: 目的研究大孔树脂分离纯化栀子豉汤有效成分的工艺条件及优化。方法采用紫外分光光度法测定总环烯醚萜苷和总大豆异黄酮的量,以总环烯醚萜苷和总大豆异黄酮的含量作为指标,采用单因素实验考察上样液浓度、上样量、洗脱剂浓度及用量等对分离纯化工艺的影响。结果最佳工艺条件:上样液最佳吸附浓度为0.1 g/ml(该浓度以每1 ml中所含生药材质量计),上样量与树脂体积比是2:1,吸附时间2 h,依次用1 BV(柱体积)水,6 BV 20%乙醇洗脱,6 BV 60%乙醇洗脱,收集洗脱液。结论该纯化工艺合理、稳定,可推广于工业生产。

English Abstract

The research of separation and purification technology for effective parts from Zhizichi decoction by macroporous absorption resina

1. College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China

2. School of Pharmacy, The Second Military Medical University, Shanghai 200433, China

3. College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China;School of Pharmacy, The Second Military Medical University, Shanghai 200433, China

Received Date: 2014-12-18
Rev Recd Date: 2015-01-28

Keywords:

Abstract: Objective To optimize separation and purification technology of Zhizichi decoction by macroporous resina. Methods The content of total iridoid glycosides and total isoflavones were determined by ultraviolet spectrophotometry. Taking the content of total iridoid glycosides and total isoflavones as indexes, the effect of the concentration of sample solution, medicinal herbs on the amount of sample, concentration and volume of eluent was investigated by single factor test. Results The optimum separation and purification technology was as following: the concentration of sample solution was 0.1 g/ml, the volume ratio of resin to material drug was 2:1, the adsorption time was 2 h, and the sample was firstly eluted with 1 BV water, then 6 BV 20% ethanol and 60% ethanol, and the eluent was collected. Conclusion This optimized separation and purification technology was reasonable and stable, and it could be extended to large-scale production applications.

韩燕, 闻俊, 周婷婷, 范国荣. 大孔吸附树脂分离纯化栀子豉汤有效部位的工艺研究[J]. 药学实践与服务, 2015, 33(3): 238-241. doi: 10.3969/j.issn.1006-0111.2015.03.012

引用本文:

韩燕, 闻俊, 周婷婷, 范国荣. 大孔吸附树脂分离纯化栀子豉汤有效部位的工艺研究[J]. 药学实践与服务, 2015, 33(3): 238-241. doi: 10.3969/j.issn.1006-0111.2015.03.012

Citation:

HAN Yan, WEN Jun, ZHOU Tingting, FAN Guorong. The research of separation and purification technology for effective parts from Zhizichi decoction by macroporous absorption resina[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(3): 238-241. doi: 10.3969/j.issn.1006-0111.2015.03.012