Keywords: 
• Juhuang Qingre San /
• heat in both exterior and interior /
• antibacterial /
• analgesic /
• anti-inflammatory /
• immune

Abstract: Objective To investigate the efficacy on antibacterial, antipyretic, analgesic, anti-inflammatory and immune regulation of Juhuang Qingre San. Methods Efficacy on antibacterial was evaluated by the method of doubling dilution,vivo antibacterial effect was observed by inoculating BABL/C mice with Streptococcus pneumonia,efficacy on antipyretic was investigated by using endotoxin fever model,analgesic function was assessed using mice hot plate and torsion analgesic method,effect of anti inflammatory was assessed with rat paw edema model and mice Peritoneal capillary permeability model, the role of regulating immune system was evaluated by mice carbon clearance test. Results Juhuang Qingre San can decrease the death rate of mice in antibacterial test in vitro or vivo,in low and mid dose group of Juhuang Qingre San, heating temperature of rabbits decreased significantly 2-3 h after administration (P<0.05), and high dosage group decreased significantly 2-4 h after administration (P<0.05),the mice pain threshold increased significantly (P<0.05) after the administration of 1.5, 2, 3 h in every group of Juhuang Qingre San was, and can significantly reduce the number of mice writhing after intraperitoneal injection (P<0.05),rat paw swelling rate decreased significantly 1, 2, 3, 4, 5 h after administration in each dosage group of Juhuang Qingre San (P<0.05), peritoneal capillary permeability decreased in the acute inflammation model of mice (P<0.05), in carbon particle clearance test of immune regulation, K value of high and medium dose group of Juhuang Qingre San was increased, while the value of alpha increased as well(P<0.05). Conclusion The results suggested that Juhuang Qingre San has efficacy of antibacterial, antipyretic, analgesic, anti-inflammatory and immune enhancing.