国产血竭总黄酮成分的纯化及活性成分的含量测定

张明媛; 宓鹤鸣; 范国荣; 陆峰; 亓云鹏

doi:10.3969/j.issn.1006-0111.2014.01.010

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国产血竭总黄酮成分的纯化及活性成分的含量测定

张明媛, 宓鹤鸣, 范国荣, 陆峰, 亓云鹏

文章导航 > 药学实践与服务 > 2014 > 32(1): 42-44

张明媛, 宓鹤鸣, 范国荣, 陆峰, 亓云鹏. 国产血竭总黄酮成分的纯化及活性成分的含量测定[J]. 药学实践与服务, 2014, 32(1): 42-44. doi: 10.3969/j.issn.1006-0111.2014.01.010

引用本文:

ZHANG Mingyuan, MI Heming, FAN Guorong, LU Feng, QI Yunpeng. Purification of total flavones by macroporous resin and its determination of the active components from Resina Draconis[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(1): 42-44. doi: 10.3969/j.issn.1006-0111.2014.01.010

Citation:

ZHANG Mingyuan, MI Heming, FAN Guorong, LU Feng, QI Yunpeng. Purification of total flavones by macroporous resin and its determination of the active components from Resina Draconis[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(1): 42-44. doi: 10.3969/j.issn.1006-0111.2014.01.010

国产血竭总黄酮成分的纯化及活性成分的含量测定

doi: 10.3969/j.issn.1006-0111.2014.01.010

1.
第二军医大学药学院药物分析学教研室, 上海 200433;福建医科大学附属南平第一医院药学部, 福建南平 353000
2.
第二军医大学药学院药物分析学教研室, 上海 200433

基金项目: 国家自然科学基金(30901981);第二军医大学药学院"时珍学者培养计划"资助.

Purification of total flavones by macroporous resin and its determination of the active components from Resina Draconis

1.
Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, China;Department of Pharmacy, the First Hospital of Naping, Fujian Medical University, Nanping 353000, China
2.
Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, China

摘要: 目的采用大孔树脂对国产血竭总黄酮进行分离纯化,并测定其中活性成分的含量。方法收集大孔树脂70%乙醇和95%乙醇洗脱液,采用高效液相色谱(HPLC)法测定其中活性成分—龙血素A、龙血素B的含量。以ODS柱为分析柱,乙腈:1%冰醋酸(34.5:65.5)为流动相,检测波长为280 nm。结果龙血素A、龙血素B分别在11.00~275.00 g/ml、20.00~500.00 g/ml范围内线性关系良好,线性方程分别为Y=35 844C+44 725(r=0.999 9),Y=28 533C-41 085(r=0.999 9);方法的准确度、精密度、稳定性均符合要求。制得的国产血竭精制总黄酮中龙血素A、龙血素B的含量分别为26.93、25.53 mg/g。结论本法可用于国产血竭中相关活性成分的分析及制备,为进一步研究国产血竭活血化瘀作用提供依据。
- 国产血竭总黄酮 /
- 大孔树脂 /
- 龙血素A /
- 龙血素B /
- HPLC
Abstract: Objective To purify the total flavones from Resina Draconis using D101 resin, and set up a method of simultaneously determining the contents of loureirin A and B in the extract. Methods Fractions in 70% and 95% ethanol were collected. Mixture of acetonitrile and l% acetic acid (34.5:65.5) was used as the mobile phase and ODS column was used as stationary phase to determine loureirin A and B. The detecting wave length was 280 nm. Results In the established HPLC method, the linear range of loureirin A was 11.00-275.00 g/ml, and that of loureirin B was 20.00-500.00 g/ml. Linear equation of loureirin A was Y=35 844C+44 725(r=0.999 9) and that of loureirin B was Y=28 533C-41 085, r=0.999 9.The accuracy, precision and stability of this method were satisfactory. Conclusion The proposed method was suitable for preparation of total flavones and determination of its active components from Resina Draconis.
- total flavones in Resina Draconis /
- macroporous resin /
- loureirin A /
- loureirin B /
- HPLC

[1]	(明)李时珍.本草纲目[M]. 北京:北京出版社, 2007:430.
[2]	陈可冀.血瘀证与活血化瘀治疗的研究[J]. 中国中医药现代远程教育, 2005, 3 (11):10-12.
[3]	曹峥.血竭的化学成分与临床应用[J]. 中国实用医药,2009, 4(2):220.
[4]	蔡辉,胡婉英.复方血竭治疗冠心病血瘀证的实验与临床研究[J]. 南京中医学院学报, 1982, 8(4):216-220.
[5]	方伟蓉, 李运曼, 邓嘉元.龙血竭总黄酮对动物心肌缺血的保护作用[J]. 中国临床药理学与治疗学, 2005, 10 (9):1020-1024.
[6]	黄树莲, 陈学芬, 陈晓军, 等.广西血竭总黄酮活血化瘀作用的研究[J]. 广西医学, 1996,18(1):1-2.
[7]	马建建,宋艳,贾敏,等.血竭总黄酮对血小板聚集、血栓形成及心肌缺血的影响[J]. 中草药, 2002, 33(11):1008-1010.
[8]	乐薇.大孔树脂精制血竭总黄酮[J]. 中南民族大学学报:自然科学版, 2008, 25(4):35-38.
[9]	屠鹏飞,王钰芳,邵杰,等.血竭中黄酮类成分提取工艺研究[J]. 中国实验方剂学杂志,2011,17(6):30-32.
[10]	朱欣婷, 刘云.大孔树脂纯化无花果叶总黄酮[J]. 中国实验方剂学杂志, 2012, 18(6):13-16.
[11]	覃金玲, 宋爱华, 黄付伟, 等.大孔树脂分离纯化青皮中总黄酮的工艺研究[J]. 中国药师, 2012, 15(8):1108-1110.
[12]	吴海霞.大孔树脂精制杭白菊总黄酮的上样条件研究[J]. 中国药业, 2012, 2l(14):67-69.
[13]	胡迎庆,李灵芝,韩慧文.RP-HPLC测定龙血竭-龙血素A和B的含量[J]. 生命科学仪器,2003,12(6):24-25.
[14]	孙胜利,宓鹤鸣,娄子洋.反相高效液相色谱法测定国产血竭中龙血素A和B的含量[J]. 第二军医大学学报,2002, 23(12):1366-1368.

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国产血竭总黄酮成分的纯化及活性成分的含量测定

doi: 10.3969/j.issn.1006-0111.2014.01.010

1. 第二军医大学药学院药物分析学教研室, 上海 200433;福建医科大学附属南平第一医院药学部, 福建南平 353000

2. 第二军医大学药学院药物分析学教研室, 上海 200433

基金项目: 国家自然科学基金(30901981);第二军医大学药学院"时珍学者培养计划"资助.

收稿日期: 2012-12-14
修回日期: 2013-09-24

关键词:

国产血竭总黄酮 /

大孔树脂 /

龙血素A /

龙血素B /

HPLC

摘要: 目的采用大孔树脂对国产血竭总黄酮进行分离纯化,并测定其中活性成分的含量。方法收集大孔树脂70%乙醇和95%乙醇洗脱液,采用高效液相色谱(HPLC)法测定其中活性成分—龙血素A、龙血素B的含量。以ODS柱为分析柱,乙腈:1%冰醋酸(34.5:65.5)为流动相,检测波长为280 nm。结果龙血素A、龙血素B分别在11.00~275.00 g/ml、20.00~500.00 g/ml范围内线性关系良好,线性方程分别为Y=35 844C+44 725(r=0.999 9),Y=28 533C-41 085(r=0.999 9);方法的准确度、精密度、稳定性均符合要求。制得的国产血竭精制总黄酮中龙血素A、龙血素B的含量分别为26.93、25.53 mg/g。结论本法可用于国产血竭中相关活性成分的分析及制备,为进一步研究国产血竭活血化瘀作用提供依据。

English Abstract

Purification of total flavones by macroporous resin and its determination of the active components from Resina Draconis

1. Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, China;Department of Pharmacy, the First Hospital of Naping, Fujian Medical University, Nanping 353000, China

2. Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, China

Received Date: 2012-12-14
Rev Recd Date: 2013-09-24

Keywords:

Abstract: Objective To purify the total flavones from Resina Draconis using D101 resin, and set up a method of simultaneously determining the contents of loureirin A and B in the extract. Methods Fractions in 70% and 95% ethanol were collected. Mixture of acetonitrile and l% acetic acid (34.5:65.5) was used as the mobile phase and ODS column was used as stationary phase to determine loureirin A and B. The detecting wave length was 280 nm. Results In the established HPLC method, the linear range of loureirin A was 11.00-275.00 g/ml, and that of loureirin B was 20.00-500.00 g/ml. Linear equation of loureirin A was Y=35 844C+44 725(r=0.999 9) and that of loureirin B was Y=28 533C-41 085, r=0.999 9.The accuracy, precision and stability of this method were satisfactory. Conclusion The proposed method was suitable for preparation of total flavones and determination of its active components from Resina Draconis.

引用本文:

Citation:

ZHANG Mingyuan, MI Heming, FAN Guorong, LU Feng, QI Yunpeng. Purification of total flavones by macroporous resin and its determination of the active components from Resina Draconis[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(1): 42-44. doi: 10.3969/j.issn.1006-0111.2014.01.010