传统中药川乌炮制前后的离子阱质谱研究
Research on constituents of Radix Aconiti and its processing by electrospray ionization trap mass spectrometry
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摘要: 目的:通过应用电喷雾-离子阱质谱,研究了炮制前后的川乌药材成分,以揭示川乌炮制减毒的原理。方法:对3种乌头对照品进行了离子阱多级质谱分析,归纳其裂解规律。对炮制前后的川乌药材进行液-质联用分析,并对各色谱峰进行归属。结果:通过对比炮制前后的质谱总离子流图及紫外色谱图,提出了川乌炮制减毒的两方面原因。结论:同时指出了将液-质联用技术引入中药炮制品的质量控制是十分必要的。Abstract: Objective : To reveal the processing principleg of preparation of Radix Aconiti by electrospray ionization trap mass spectrometry. Methods : The three reference standars were made MSn analysis and elucidating the fragmentation pathways.The rapid analysis by HPLC-ESI-MS had been used for identification of contituents in Radix Aconiti. Results :The comparison of mass spectra and liquid chromatographic information revealed the differences of Radix Aconiti and its process product. Conclusion : It is necessary to make quality control of process product of traditional Chinese herbal medicines by HPLC-ESI-MS.
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