夏枯草消瘤合剂对Lewis肺癌小鼠的药效作用

马明华; 汪晓河; 戴媛媛; 顾小燕; 吴铁军; 年华

doi:10.3969/j.issn.1006-0111.201812070

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

x 关闭永久关闭

应中央军委要求，2022年9月起，《药学实践杂志》将更名为《药学实践与服务》，双月刊，正文96页；2023年1月起，拟出版月刊，正文64页，数据库收录情况与原《药学实践杂志》相同。欢迎作者踊跃投稿！

夏枯草消瘤合剂对Lewis肺癌小鼠的药效作用

马明华, 汪晓河, 戴媛媛, 顾小燕, 吴铁军, 年华

文章导航 > 药学实践与服务 > 2020 > 38(1): 57-62

马明华, 汪晓河, 戴媛媛, 顾小燕, 吴铁军, 年华. 夏枯草消瘤合剂对Lewis肺癌小鼠的药效作用[J]. 药学实践与服务, 2020, 38(1): 57-62. doi: 10.3969/j.issn.1006-0111.201812070

引用本文:

MA Minghua, WANG Xiaohe, DAI Yuanyuan, GU Xiaoyan, WU Tiejun, NIAN Hua. Therapeutic effect of Xiakucao Xiaoliu mixture on Lewis lung cancer mice[J]. Journal of Pharmaceutical Practice and Service, 2020, 38(1): 57-62. doi: 10.3969/j.issn.1006-0111.201812070

Citation:

MA Minghua, WANG Xiaohe, DAI Yuanyuan, GU Xiaoyan, WU Tiejun, NIAN Hua. Therapeutic effect of Xiakucao Xiaoliu mixture on Lewis lung cancer mice[J]. Journal of Pharmaceutical Practice and Service, 2020, 38(1): 57-62. doi: 10.3969/j.issn.1006-0111.201812070

夏枯草消瘤合剂对Lewis肺癌小鼠的药效作用

doi: 10.3969/j.issn.1006-0111.201812070

1.
上海市杨浦区中心医院药剂科, 上海 200090
2.
上海中医药大学附属岳阳中西医结合医院药剂科, 上海 200437

基金项目: 上海中医药大学高峰高原学科2018年项目（30304114334）；中药炮制传承基地项目（30304118220）；上海市科学技术委员会科研计划项目（16401902100）；上海中医药大学预算内项目（18LK047）

Therapeutic effect of Xiakucao Xiaoliu mixture on Lewis lung cancer mice

1.
Pharmaceutical Center of Yangpu District Central Hospital, Shanghai 200090, China
2.
Pharmaceutical Center of Yueyang Hospital, Shanghai University of TCM, Shanghai 200437, China

摘要: 目的探讨夏枯草消瘤合剂对Lewis肺癌小鼠的治疗作用。方法采用C₅₇BL/6小鼠Lewis肺癌移植瘤模型，将30只小鼠随机分为3组：模型对照组、夏枯草消瘤合剂组（M组）、顺铂组（DDP组），连续用药14 d。通过对Lewis肺癌小鼠的大体观察，测定肿瘤大小，HE染色方法测定肿瘤病理组织学变化，免疫组化方法测定肿瘤组织的细胞周期蛋白D1（CyclinD1）和P16的表达情况。结果模型对照组瘤体质量最重，与其他组比较差异均有统计学意义（P<0.05）；M组可在一定程度上改善小鼠的生存状态及生活质量；各组小鼠的肿瘤生长曲线及HE染色结果显示：M组可抑制肿瘤细胞的生长，且对正常细胞有保护作用；M组、DDP组均可显著降低CyclinD1阳性表达（P<0.01），但是M组对于提高P16阳性表达的效果不显著。结论夏枯草消瘤合剂具有较好的抑制肺癌生长的作用。
- 中医药 /
- 夏枯草消瘤合剂 /
- Lewis肺癌 /
- CyclinD1 /
- P16
Abstract: Objective To investigate the therapeutic effect of Xiakucao Xiaoliu mixture on Lewis lung cancer mice. Methods 30 mice with C₅₇BL/6 mouse Lewis lung cancer xenograft model were randomly divided into three groups:model control group, Xiakucao Xiaoliu mixture group (M group), cisplatin group (DDP group). M group and DDP group were administered continuously for 14 days. Through the general observation of Lewis lung cancer mice, tumor size was determined, HE staining method was used to determine the histopathological changes of tumors, and the expression of CyclinD1 and P16 in tumor tissues was determined by immunohistochemistry. Results The tumor weight of the model control group was the heaviest, and the difference was statistically significant compared with other groups. (P<0.05). Survival state and quality of life of mice had been improved to some extent in M group. The results of tumor growth curve and HE staining in each group of mice showed that the growth of tumor cells had been inhibited and normal cells had been protected. The positive expression of CyclinD1 was significantly decreased in M group and DDP group (P<0.01), but the effect of M group on the improvement of P16 positive expression was not significant. Conclusion Xiakucao Xiaoliu mixture had a good effect on inhibiting lung tumor growth.
- Chinese Medicine /
- Xiakucao Xiaoliu mixture /
- Lewis lung cancer /
- CyclinD1 /
- P16

[1]	TORRE L A, BRAY F, SIEGEL R L, et al. Global cancer statistics, 2012[J]. CA: Cancer J Clin,2015,65(2):87-108
[2]	赵东杰. 中西医结合疗法对老年晚期非小细胞肺癌临床疗效的影响[J]. 中外医疗, 2017, 36(9):167-169
[3]	王丽丽, 石磊, 钱祥, 等. 化疗联合清肺合剂改善非小细胞肺癌患者免疫功能的临床研究[J]. 中华全科医学, 2017, 15(7):1166-1168
[4]	尚兴平, 齐敏. 益气养阴法联合化疗治疗晚期非小细胞肺癌的疗效观察[J]. 中国继续医学教育, 2017, 9(21):203-205
[5]	柯军. 迷迭香酸抑制肺癌A549细胞的增殖及侵袭的实验研究[J]. 甘肃医药, 2015, 34(4):255-258
[6]	贺天临, 谢国群, 陈洁, 等. 夏枯草消瘤合剂联合化疗治疗中晚期非小细胞肺癌30例[J]. 上海中医药大学学报, 2015, 29(5):29-32
[7]	马雪曼, 于明薇, 张甘霖, 等. 小鼠Lewis肺癌不同部位皮下移植瘤模型的比较[J]. 中国实验动物学报, 2017, 25(4):386-390
[8]	靳福鹏, 张梅, 李平, 等. 益气养阴解毒方对小鼠Lewis肺癌生长抑制作用的实验研究[J]. 临床肺科杂志, 2011, 16(1):72-74
[9]	钱超尘, 温长路,赵怀舟,等(校). 金陵本《本草纲目》新校正[M]. 上海: 上海科学技术出版社, 2008.
[10]	严东, 谢文剑, 李春, 等. 夏枯草化学成分及其体外抗肿瘤活性研究[J]. 中国实验方剂学杂志, 2016, 22(11):49-54
[11]	张朝晖, 徐正顺, 焦龙华, 等. CyclinD1, P16, Rb蛋白在非小细胞肺癌组织中的表达[J]. 第四军医大学学报, 2003, 24(14):1271-1274
[12]	王前, 邓晶, 蒋永新. CyclinD1的研究进展[J]. 现代肿瘤医学, 2009, 17(2):350-353
[13]	CHOI Y J, LI X Y, HYDBRING P, et al. The requirement for Cyclin D function in tumor maintenance[J]. Cancer Cell,2012,22(4):438-451
[14]	MASAMHA C P, BENBROOK D M. Cyclin D1 degradation is sufficient to induce G1 cell cycle arrest despite constitutive expression of Cyclin E2 in ovarian cancer cells[J]. Cancer Res,2009,69(16):6565-6572
[15]	OGINO S, NOSHO K, IRAHARA N, et al. A cohort study of Cyclin D1 expression and prognosis in 602 colon cancer cases[J]. Clin Cancer Res,2009,15(13):4431-4438
[16]	ISHIKAWA T, FURIHATA M, OHTSUKI Y, et al. Cyclin D1 overexpression related to retinoblastoma protein expression as a prognostic marker in human oesophageal squamous cell carcinoma[J]. Br J Cancer,1998,77(1):92-97
[17]	LI H Y, XIAO W W, MA J W, et al. Dual high expression of STAT3 and CyclinD1 is associated with poor prognosis after curative resection of esophageal squamous cell carcinoma[J]. Int J Clin Exp Pathol,2014,7(11):7989-7998
[18]	夏靖华, 李维妙, 王雪娇, 等. CyclinD1与CyclinD3在非小细胞肺癌中的表达及意义[J]. 肿瘤学杂志, 2016, 22(8):615-621
[19]	梁瑞韵, 廖增顺, 江山平, 等. CyclinD1和VEGF在非小细胞肺癌组织中的表达及其与预后的关系[J]. 癌症, 2003, 22(1):86-90
[20]	付冉, 王艳林, 黄利鸣. p16表达异常与肿瘤关系的研究进展[J]. 生命的化学, 2017, 37(4):585-589
[21]	冯蓓, 冯艳, 杨小平, 等. P16在肺癌方面的研究进展[J]. 延安大学学报(医学科学版), 2015, 13(1):70-73

[1]	丁华敏, 郭羽晨, 秦春霞, 宋志兵, 孙莉莉. 消风止痒颗粒通过降低白三烯水平对小鼠特应性皮炎急性瘙痒的治疗作用研究 . 药学实践与服务, 2024, 42(5): 211-216. doi: 10.12206/j.issn.2097-2024.202306031
[2]	何亚伦, 祁智, 常杰. 消胀通便膏在晚期肝癌患者阿片类药物相关性便秘中的应用研究 . 药学实践与服务, 2024, 42(12): 520-523. doi: 10.12206/j.issn.2097-2024.202309009
[3]	岳春华, 贲永光, 王海桥. 基于NLRP1炎症小体探讨百合知母汤抗抑郁的作用机制 . 药学实践与服务, 2024, 42(8): 325-333. doi: 10.12206/j.issn.2097-2024.202401033
[4]	李想, 陆鸿远, 张明玉, 高欢, 姚东, 许子华. 米格列醇激活UCP1介导棕色脂肪对冷暴露小鼠损伤的研究 . 药学实践与服务, 2024, 42(): 1-6. doi: 10.12206/j.issn.2097-2024.202404005
[5]	王晓飞, 张颖, 顾佳钰, 胡馨儿, 张海, 曹岩. 表面等离子共振传感器的识别元件在医药领域中的研究应用进展 . 药学实践与服务, 2024, 42(): 1-9. doi: 10.12206/j.issn.2097-2024.202309014
[6]	唐淑慧, 凤美娟, 薛智霞, 鲁桂华. 帕博利珠单抗治疗所致免疫相关不良反应与中医体质的相关性研究 . 药学实践与服务, 2024, 42(5): 217-222. doi: 10.12206/j.issn.2097-2024.202311029
[7]	刘丽艳, 余小翠, 孙传铎. 纳武利尤单抗治疗非小细胞肺癌有效性及安全性的Meta分析 . 药学实践与服务, 2024, 42(10): 451-456. doi: 10.12206/j.issn.2097-2024.202310044
[8]	宋雨桐, 夏德润, 顾珩, 唐少文, 易洪刚, 沃红梅. 帕博利珠单抗与铂类化疗方案在晚期非小细胞肺癌一线治疗中的药物经济学评价 . 药学实践与服务, 2024, 42(8): 334-340. doi: 10.12206/j.issn.2097-2024.202303023
[9]	杨嘉宁, 赵一颖, 肖伟. 七味脂肝方对非酒精性脂肪性肝炎动物模型的药效学评价 . 药学实践与服务, 2024, 42(9): 389-398. doi: 10.12206/j.issn.2097-2024.202404096

点击查看大图

计量

文章访问数: 5349
HTML全文浏览量: 553
PDF下载量: 3363
被引次数: 0

夏枯草消瘤合剂对Lewis肺癌小鼠的药效作用

doi: 10.3969/j.issn.1006-0111.201812070

1. 上海市杨浦区中心医院药剂科, 上海 200090

2. 上海中医药大学附属岳阳中西医结合医院药剂科, 上海 200437

收稿日期: 2018-12-24
修回日期: 2019-09-22

关键词:

中医药 /

夏枯草消瘤合剂 /

Lewis肺癌 /

CyclinD1 /

P16

摘要: 目的探讨夏枯草消瘤合剂对Lewis肺癌小鼠的治疗作用。方法采用C₅₇BL/6小鼠Lewis肺癌移植瘤模型，将30只小鼠随机分为3组：模型对照组、夏枯草消瘤合剂组（M组）、顺铂组（DDP组），连续用药14 d。通过对Lewis肺癌小鼠的大体观察，测定肿瘤大小，HE染色方法测定肿瘤病理组织学变化，免疫组化方法测定肿瘤组织的细胞周期蛋白D1（CyclinD1）和P16的表达情况。结果模型对照组瘤体质量最重，与其他组比较差异均有统计学意义（P<0.05）；M组可在一定程度上改善小鼠的生存状态及生活质量；各组小鼠的肿瘤生长曲线及HE染色结果显示：M组可抑制肿瘤细胞的生长，且对正常细胞有保护作用；M组、DDP组均可显著降低CyclinD1阳性表达（P<0.01），但是M组对于提高P16阳性表达的效果不显著。结论夏枯草消瘤合剂具有较好的抑制肺癌生长的作用。

English Abstract

Therapeutic effect of Xiakucao Xiaoliu mixture on Lewis lung cancer mice

1. Pharmaceutical Center of Yangpu District Central Hospital, Shanghai 200090, China

2. Pharmaceutical Center of Yueyang Hospital, Shanghai University of TCM, Shanghai 200437, China

Received Date: 2018-12-24
Rev Recd Date: 2019-09-22

Keywords:

Abstract: Objective To investigate the therapeutic effect of Xiakucao Xiaoliu mixture on Lewis lung cancer mice. Methods 30 mice with C₅₇BL/6 mouse Lewis lung cancer xenograft model were randomly divided into three groups:model control group, Xiakucao Xiaoliu mixture group (M group), cisplatin group (DDP group). M group and DDP group were administered continuously for 14 days. Through the general observation of Lewis lung cancer mice, tumor size was determined, HE staining method was used to determine the histopathological changes of tumors, and the expression of CyclinD1 and P16 in tumor tissues was determined by immunohistochemistry. Results The tumor weight of the model control group was the heaviest, and the difference was statistically significant compared with other groups. (P<0.05). Survival state and quality of life of mice had been improved to some extent in M group. The results of tumor growth curve and HE staining in each group of mice showed that the growth of tumor cells had been inhibited and normal cells had been protected. The positive expression of CyclinD1 was significantly decreased in M group and DDP group (P<0.01), but the effect of M group on the improvement of P16 positive expression was not significant. Conclusion Xiakucao Xiaoliu mixture had a good effect on inhibiting lung tumor growth.