Quality control standard for Qingre Baidu granules
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摘要: 目的 建立清热败毒颗粒剂的质量标准。 方法 采用薄层色谱法（TLC）鉴别处方中的板蓝根、连翘、紫花地丁和甘草，采用高效液相色谱法（HPLC）测定绿原酸的含量，色谱柱为Agilent ZORBAX SB-C18柱（4.6 mm×250 mm，5 μm）；流动相为乙腈（A）与含0.2%甲酸的水溶液（B），梯度洗脱：0~12 min，11%~12%A；流速：1.0 ml/min；柱温：30 ℃；检测波长：327 nm；进样量：20 μl。 结果 薄层鉴别色谱中特征斑点明显，专属性强。绿原酸在6.19~396.00 μg/ml浓度范围内呈良好线性关系，其回归方程为Y=60.239 4X+9.096 3（r>0.999 9），回收率为99.66%（RSD=2.82%）。 结论 该法简便、稳定可靠、重复性好，为控制清热败毒颗粒的质量标准提供了可借鉴的依据。Abstract: Objective To establish a quality control standard for Qingre Baidu granules. Methods Isatidis Radix, Fructus Forsythiae,Herba Violae, and Glycyrrhizae were identified by TLC, and the concentration of chlorogenic acid was determined by HPLC. This method was employed on an Agilent ZORBAX SB-C18 column (4.6 mm×250 mm, 5 μm) at 30℃ with a mobile phase of acetonitrile (A) and 0.2% formic acid (B) using the gradient elution program shown as follows: 0-12 min, 11%-12% A run at the flow rate of 1.0 ml/min. The injection volume was 20 μl and the detection wavelength was 327 nm. Results Characteristic spots could be detected by TLC and the specificity of the method was satisfactory. As for chlorogenic acid, the equation of linear regression of chlorogenic acid was Y=60.239 4X+9.096 3 (r=0.999 9) with the linear range of 6.19-396.00 μg/ml. The average recovery was 99.66% (RSD=2.82%). Conclusion The established method is simple, reliable, reproducible, and can be used for the quantitative determination and quality control of Qingre Baidu granules.
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Key words:
- HPLC /
- TLC /
- chlorogenic acid /
- Qingre Baidu granules
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[1] 汤迎爽, 李富军. 浅议军队医院制剂发展方向[J]. 中医药导报, 2012,18(7):120-121. [2] 周苏萍, 夏晓东, 傅小英. 军队药品制剂抽验质量分析与建议[J]. 解放军药学学报, 2010,26(2):176-177. [3] 黄立峰, 宋洪涛. 目前形势下医院制剂在大型军队医院中的存在与发展[J]. 解放军药学学报, 2006, 22(1): 77-79. [4] 李 霞, 杨颖博, 李君丽, 等. HPLC法同时测定皱皮木瓜中原儿茶酸和绿原酸的含量[J]. 中国医药导报, 2012, 9(34):110-112. [5] 赵金娟, 戴雪梅, 曲永胜, 等. 绿原酸药效学研究进展[J]. 中国野生植物资源, 2013, 32(4):1-5. [6] 吴卫华, 康 桢, 欧阳冬生, 等. 绿原酸的药理学研究进展[J]. 天然产物研究与开发, 2006, 18:691-694. [7] 王丽萍, 郭 栋, 王 果, 等. 中药绿原酸的研究进展[J]. 时珍国医国药, 2011, 22(4):961-963. [8] Cai Y, Zhang ZH, Jiang SS, et al. Chlorogenic acid increased acrylamide formation through promotion of HMF formation and 3-aminopropionamide deamination[J]. J Hazard Mater, 2014, 268:1-5. [9] Francisco V, Costa G, Figueirinha A, et al. Anti-inflammatory activity of Cymbopogon citratus leaves infusion via proteasome and nuclear factor-κB pathway inhibition: contribution of chlorogenic acid [J]. J Ethnopharmacol, 2013, 148(1):126-134. -
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