Keywords: 
• methylphenidate hydrochloride /
• anti-fatigue /
• load-weighted swimming test /
• lactate dehydrogenase /
• creatine kinase /
• triglycerides

Abstract: Objective To study the anti-fatigue effect of methylphenidate hydrochloride oral fast dissolving films (MPH-OFDF) and its mechanism. Methods 60 mice were randomly divided into 6 groups as: normal control group (physiological saline), model group (physiological saline), Yiqiyangxue oral liquids positive group (7.00 mg/kg), MPH-OFDF high-dose group (5.20 mg/kg), MPH-OFDF middle-dose group (2.60 mg/kg) and MPH-OFDF low-dose group (1.30 mg/kg). Besides the normal control group,model group and positive group were orally administered, the other groups are administered with the drug once daily sublingually daily for consecutive 15 days. The mice were put in the load-weighted swimming test 30 min after the last oral administration, then the anti-fatigue effect was assessed based on recording exhausting swimming time and detecting the levels of serum lactale dehydrogenase (LDH), creatine kinase (CK), triglycerides (TG) in mice. Results Compared with control group, the middle-dose and the high-dose MPH could prolong the exhausting swimming time (P<0.05,P<0.01) and decrease the activity of LDH and CK significantly (P<0.05,P<0.01);in addition the middle-dose MPH could decrease the content of TG (P<0.05). Conclusion The MPH had marked anti-fatigue effect that may be associated with reduced serum LDH, CK and TG.