Study of anti-fatigue effect of methylphenidate hydrochloride oral fast dissolving films and its mechanism
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摘要: 目的 研究盐酸哌甲酯(methylphenidate hydrochloride, MPH)口腔速溶膜(oral fast dissolving films, OFDF)抗疲劳作用及其机制。 方法 将60只小鼠随机分为6组:正常对照组(生理盐水)、游泳对照组(生理盐水)、益气养血口服液阳性组(7 mg/kg)、MPH-OFDF高、中、低剂量组(5.2、 2.6、1.3 mg/kg)。正常对照组、游泳对照组及阳性组小鼠予以灌胃,其他组小鼠每天舌下给药1次,连续15 d,末次给药30 min后进行负重游泳实验,记录小鼠游泳至力竭的时间,并检测小鼠血清乳酸脱氢酶(LDH)、肌酸激酶(CK)和三酰甘油(TG)水平。 结果 与游泳对照组比较,高、中剂量MPH-OFDF可显著延长小鼠的力竭性游泳时间(P<0.05,P<0.01),降低小鼠血清中LDH、CK水平(P<0.05,P<0.01),且中剂量MPH能降低小鼠血清的TG含量(P<0.05)。 结论 MPH-OFDF具有明显的抗疲劳作用,其机制可能与降低血清LDH、CK及TG含量有关。Abstract: Objective To study the anti-fatigue effect of methylphenidate hydrochloride oral fast dissolving films (MPH-OFDF) and its mechanism. Methods 60 mice were randomly divided into 6 groups as: normal control group (physiological saline), model group (physiological saline), Yiqiyangxue oral liquids positive group (7.00 mg/kg), MPH-OFDF high-dose group (5.20 mg/kg), MPH-OFDF middle-dose group (2.60 mg/kg) and MPH-OFDF low-dose group (1.30 mg/kg). Besides the normal control group,model group and positive group were orally administered, the other groups are administered with the drug once daily sublingually daily for consecutive 15 days. The mice were put in the load-weighted swimming test 30 min after the last oral administration, then the anti-fatigue effect was assessed based on recording exhausting swimming time and detecting the levels of serum lactale dehydrogenase (LDH), creatine kinase (CK), triglycerides (TG) in mice. Results Compared with control group, the middle-dose and the high-dose MPH could prolong the exhausting swimming time (P<0.05,P<0.01) and decrease the activity of LDH and CK significantly (P<0.05,P<0.01);in addition the middle-dose MPH could decrease the content of TG (P<0.05). Conclusion The MPH had marked anti-fatigue effect that may be associated with reduced serum LDH, CK and TG.
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