LC-MS/MS测定大鼠血浆中虎杖苷浓度

丁雪鹰; 侯晓丽; 孙铭学; 肖凯

doi:10.3969/j.issn.1006-0111.2012.01.012

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

x 关闭永久关闭

应中央军委要求，2022年9月起，《药学实践杂志》将更名为《药学实践与服务》，双月刊，正文96页；2023年1月起，拟出版月刊，正文64页，数据库收录情况与原《药学实践杂志》相同。欢迎作者踊跃投稿！

LC-MS/MS测定大鼠血浆中虎杖苷浓度

丁雪鹰, 侯晓丽, 孙铭学, 肖凯

文章导航 > 药学实践与服务 > 2012 > 30(1): 45-48

丁雪鹰, 侯晓丽, 孙铭学, 肖凯. LC-MS/MS测定大鼠血浆中虎杖苷浓度[J]. 药学实践与服务, 2012, 30(1): 45-48. doi: 10.3969/j.issn.1006-0111.2012.01.012

引用本文:

丁雪鹰, 侯晓丽, 孙铭学, 肖凯. LC-MS/MS测定大鼠血浆中虎杖苷浓度[J]. 药学实践与服务, 2012, 30(1): 45-48. doi: 10.3969/j.issn.1006-0111.2012.01.012

DING Xue-ying, HOU Xiao-li, SUN Ming-xue, XIAO Kai. Determination of polydatin in rats plasma by LC-MS/MS[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(1): 45-48. doi: 10.3969/j.issn.1006-0111.2012.01.012

Citation:

DING Xue-ying, HOU Xiao-li, SUN Ming-xue, XIAO Kai. Determination of polydatin in rats plasma by LC-MS/MS[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(1): 45-48. doi: 10.3969/j.issn.1006-0111.2012.01.012

LC-MS/MS测定大鼠血浆中虎杖苷浓度

doi: 10.3969/j.issn.1006-0111.2012.01.012

1.
第二军医大学药学院药剂学教研室,上海 200433
2.
第二军医大学海医系毒物药物研究室,上海 200433

基金项目: 上海市科委中药现代化项目(08DZ1971504);国家自然科学基金(20872179,30472141).

Determination of polydatin in rats plasma by LC-MS/MS

1.
Department of Pharmaceutics, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
2.
Laboratory of Toxicology & Pharmacology, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China

摘要: 目的建立测定大鼠血浆中虎杖苷浓度的LC-MS/MS方法。方法采用LC-MS/MS测定大鼠血浆中虎杖苷的浓度,以二苯乙烯苷为内标,血浆样品用乙腈沉淀蛋白,色谱柱为Agilent Zorbax SB-C₁₈ (100 mm×2.1 mm, 3.5 μm),流动相为甲醇-乙腈-0.1%甲酸水溶液(18∶15∶67),流速0.3 ml/min,柱温30 ℃。结果虎杖苷的线性范围为1.0~5 000.0 ng/ml (r=0.998 4),最低定量检测浓度为1.0
- 虎杖苷 /
- LC-MS/MS /
- 血药浓度
Abstract: Objective To establish a LC-MS/MS method for determination of polydatin concentration in rats plasma. Methods The plasma concentration of polydatin was determined by LC-MS/MS. Using stilbene glucoside as internal standard, after precipitation of the plasma proteins with methanol, the analytes were separated on an agilent zorbax SB-C₁₈ reversed-phase column with methanol-acetonitrile- 0.1% formic acid (18 : 15 : 67, v/v) and detected by electrospray ionization (ESI) mass spectrometry in negative multiple reaction monitoring (MRM) mode. The flow rate was 0.3 ml/min. Column temperature was maintained at 30 ℃. Results The calibration curves with good linearity (r=0.998 4 for plasma sample) were obtained in the range of 1.0~5000.0 ng/ml for polydatin. The lower limit of quantification (LLOQ) was 1.0 ng/ml. Recoveries were around 78% for the extraction from rats plasma, and good precision and accuracy were achieved. The intra-day and inter-day RSD were both less than 15.0%. This method was feasible for the evaluation of pharmacokinetic profiles of polydatin in rats. Conclusion This method was simple, selective and sensitive. It's suitable for the pharmacokinetic research of polydatin in rats plasma.
- polydatin /
- LC-MS/MS /
- plasma drug concentration

[1]	肖凯,宣利江,徐亚明,等.虎杖的化学成分研究[J].中国药学杂志, 2003,38(1):12.
[2]	高守红,杨少麟,范国荣. 虎杖苷的研究进展[J].药学实践杂志,2005,23(3):145.
[3]	李蓟龙,尹京湘,张力,等. 虎杖中白藜芦醇对豚鼠左心室流出道自律组织电活动的影响[J]. 中国应用生理学杂志,2010,26(3):311,364.
[4]	刘铭,刘华,张国平,等. 虎杖苷对脑出血性损伤大鼠血清白细胞介素-1β和脑组织Bcl-2蛋白表达的影响[J]. 中草药,2010, 41(12):2010.
[5]	Gao S, Fan G, Hong Z, et al. HPLC determination of polydatin in rat biological matrices: application to pharmacokinetic studies[J]. J Pharmaceut Biomed,2006, 41(1): 240.
[6]	吕春艳,张兰桐,袁志芳,等. 虎杖苷在大鼠体内的药动学特点和组织分布研究[J].中草药,2006,38(2):235.
[7]	林建海,李笑宏,唐跃年,等. 白藜芦醇苷的HPLC法分析及其在苗猪体内的药代动力学研究[J]. 药物分析杂志, 2001,21(05):325.
[8]	杨润涛,周四元,章翔,等.液相色谱-串联质谱法同时测定血浆中白藜芦醇苷及其代谢产物[J]. 分析化学,2007,35(9):1309.
[9]	Zhou S, Yang R,Teng Z,et al. Dose-dependent absorption and metabolism of trans-polydatin in rats[J]. J Agr Food Chem, 2009,57(11): 4572.

点击查看大图

计量

文章访问数: 4284
HTML全文浏览量: 231
PDF下载量: 395
被引次数: 0

LC-MS/MS测定大鼠血浆中虎杖苷浓度

doi: 10.3969/j.issn.1006-0111.2012.01.012

1. 第二军医大学药学院药剂学教研室,上海 200433

2. 第二军医大学海医系毒物药物研究室,上海 200433

基金项目: 上海市科委中药现代化项目(08DZ1971504);国家自然科学基金(20872179,30472141).

收稿日期: 2011-02-10
修回日期: 2011-12-21

关键词:

虎杖苷 /

LC-MS/MS /

血药浓度

摘要: 目的建立测定大鼠血浆中虎杖苷浓度的LC-MS/MS方法。方法采用LC-MS/MS测定大鼠血浆中虎杖苷的浓度,以二苯乙烯苷为内标,血浆样品用乙腈沉淀蛋白,色谱柱为Agilent Zorbax SB-C₁₈ (100 mm×2.1 mm, 3.5 μm),流动相为甲醇-乙腈-0.1%甲酸水溶液(18∶15∶67),流速0.3 ml/min,柱温30 ℃。结果虎杖苷的线性范围为1.0~5 000.0 ng/ml (r=0.998 4),最低定量检测浓度为1.0

English Abstract

Determination of polydatin in rats plasma by LC-MS/MS

1. Department of Pharmaceutics, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

2. Laboratory of Toxicology & Pharmacology, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China

Received Date: 2011-02-10
Rev Recd Date: 2011-12-21

Keywords:

Abstract: Objective To establish a LC-MS/MS method for determination of polydatin concentration in rats plasma. Methods The plasma concentration of polydatin was determined by LC-MS/MS. Using stilbene glucoside as internal standard, after precipitation of the plasma proteins with methanol, the analytes were separated on an agilent zorbax SB-C₁₈ reversed-phase column with methanol-acetonitrile- 0.1% formic acid (18 : 15 : 67, v/v) and detected by electrospray ionization (ESI) mass spectrometry in negative multiple reaction monitoring (MRM) mode. The flow rate was 0.3 ml/min. Column temperature was maintained at 30 ℃. Results The calibration curves with good linearity (r=0.998 4 for plasma sample) were obtained in the range of 1.0~5000.0 ng/ml for polydatin. The lower limit of quantification (LLOQ) was 1.0 ng/ml. Recoveries were around 78% for the extraction from rats plasma, and good precision and accuracy were achieved. The intra-day and inter-day RSD were both less than 15.0%. This method was feasible for the evaluation of pharmacokinetic profiles of polydatin in rats. Conclusion This method was simple, selective and sensitive. It's suitable for the pharmacokinetic research of polydatin in rats plasma.

丁雪鹰, 侯晓丽, 孙铭学, 肖凯. LC-MS/MS测定大鼠血浆中虎杖苷浓度[J]. 药学实践与服务, 2012, 30(1): 45-48. doi: 10.3969/j.issn.1006-0111.2012.01.012

引用本文:

丁雪鹰, 侯晓丽, 孙铭学, 肖凯. LC-MS/MS测定大鼠血浆中虎杖苷浓度[J]. 药学实践与服务, 2012, 30(1): 45-48. doi: 10.3969/j.issn.1006-0111.2012.01.012

Citation:

全文HTML

参考文献 (9)

下载: 全尺寸图片幻灯片

返回文章

用微信扫码二维码

分享至好友和朋友圈

主管、主办: 中国人民解放军海军军医大学地址: 上海市杨浦区国和路325号邮政编码: 200433

电话: （021）81871324,81871397 E-mail: yxsjzzs@163.com

网站备案号沪ICP备05003363号-1

本系统由北京仁和汇智信息技术有限公司开发

[1]	景凯, 杨慈荣, 张圳, 臧艺蓓, 刘霞. 黄芪甲苷衍生物治疗慢性心力衰竭小鼠的药效评价及作用机制研究 . 药学实践与服务, 2024, 42(5): 190-197. doi: 10.12206/j.issn.2097-2024.202310004
[2]	陈炳辰, 佟达丰, 万苗, 闫飞虎, 姚建忠. UPLC-MS/MS法测定小鼠血浆中紫杉醇脂肪酸酯前药及其药代动力学研究 . 药学实践与服务, 2024, 42(8): 341-345. doi: 10.12206/j.issn.2097-2024.202404082
[3]	陈方剑, 骆锦前, 王志君, 胡叶帅, 孙煜昕, 宋洪杰. HPLC-MS/MS同时测定感冒安颗粒中5种黄酮成分的含量 . 药学实践与服务, 2024, 42(9): 402-406. doi: 10.12206/j.issn.2097-2024.202403030
[4]	李丹, 戴贤春, 王芳珍, 陈奕含, 杨萍, 刘继勇. HPLC-MS/MS测定当归六黄汤中4种不同成分的含量 . 药学实践与服务, 2024, 42(6): 248-252, 266. doi: 10.12206/j.issn.2097-2024.202305007
[5]	桂明珠, 李静, 李志玲. 儿童伏立康唑的血药浓度与CYP2C19、CYP2C9和CYP3A5基因多态性的相关性研究 . 药学实践与服务, 2024, 42(): 1-5. doi: 10.12206/j.issn.2097-2024.202402020

留言板

LC-MS/MS测定大鼠血浆中虎杖苷浓度

doi: 10.3969/j.issn.1006-0111.2012.01.012

Determination of polydatin in rats plasma by LC-MS/MS

计量

出版历程

LC-MS/MS测定大鼠血浆中虎杖苷浓度

doi: 10.3969/j.issn.1006-0111.2012.01.012

1. 第二军医大学药学院药剂学教研室,上海 200433 2. 第二军医大学海医系毒物药物研究室,上海 200433

English Abstract

Determination of polydatin in rats plasma by LC-MS/MS

1. Department of Pharmaceutics, School of Pharmacy, Second Military Medical University, Shanghai 200433, China 2. Laboratory of Toxicology & Pharmacology, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China

全文HTML

目录

1. 第二军医大学药学院药剂学教研室,上海 200433

2. 第二军医大学海医系毒物药物研究室,上海 200433

1. Department of Pharmaceutics, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

2. Laboratory of Toxicology & Pharmacology, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China