啤酒花HPLC指纹图谱研究

董志敏; 许月明; 闻俊; 蒋益萍; 秦路平; 辛海量

doi:10.3969/j.issn.1006-0111.2019.01.010

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啤酒花HPLC指纹图谱研究

董志敏, 许月明, 闻俊, 蒋益萍, 秦路平, 辛海量

文章导航 > 药学实践与服务 > 2019 > 37(1): 42-45,54

董志敏, 许月明, 闻俊, 蒋益萍, 秦路平, 辛海量. 啤酒花HPLC指纹图谱研究[J]. 药学实践与服务, 2019, 37(1): 42-45,54. doi: 10.3969/j.issn.1006-0111.2019.01.010

引用本文:

董志敏, 许月明, 闻俊, 蒋益萍, 秦路平, 辛海量. 啤酒花HPLC指纹图谱研究[J]. 药学实践与服务, 2019, 37(1): 42-45,54. doi: 10.3969/j.issn.1006-0111.2019.01.010

DONG Zhimin, XU Yueming, WEN Jun, JIANG Yiping, QIN Luping, XIN Hailiang. Study on fingerprint of Hupulus lupulus by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(1): 42-45,54. doi: 10.3969/j.issn.1006-0111.2019.01.010

Citation:

DONG Zhimin, XU Yueming, WEN Jun, JIANG Yiping, QIN Luping, XIN Hailiang. Study on fingerprint of Hupulus lupulus by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(1): 42-45,54. doi: 10.3969/j.issn.1006-0111.2019.01.010

啤酒花HPLC指纹图谱研究

doi: 10.3969/j.issn.1006-0111.2019.01.010

1.
上海师范大学生命与环境科学学院, 上海 200234;海军军医大学药学院, 上海 200433
2.
海军军医大学药学院, 上海 200433
3.
上海师范大学生命与环境科学学院, 上海 200234;海军军医大学药学院, 上海 200433;浙江中医药大学药学院, 浙江杭州 310053

基金项目: 国家自然科学基金（U1603283）

Study on fingerprint of Hupulus lupulus by HPLC

1.
College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China;School of Pharmacy, Naval Medical University, Shanghai 200433, China
2.
School of Pharmacy, Naval Medical University, Shanghai 200433, China
3.
College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China;School of Pharmacy, Naval Medical University, Shanghai 200433, China;College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310053, China

摘要: 目的建立啤酒花HPLC指纹图谱。方法色谱柱：Diamonsil C₁₈柱（250 mm×4.6 mm，5 μm），流动相：乙腈-0.1%磷酸水，梯度洗脱，检测波长358 nm，柱压10.2 MPa，柱温30℃，进样量10 μl，流速1.0 ml/min。结果首次建立啤酒花HPLC指纹图谱共有模式，对其19个共有峰进行标定，并对其中的黄腐酚、类葎草酮、葎草酮、加葎草酮、类蛇麻酮、蛇麻酮、加蛇麻酮进行指认。26批药材中有5批药材相似度低于或等于0.9，而该5批均为野生品种。结论该方法高速有效，可准确地对啤酒花栽培种、野生种进行区分，为啤酒花的品质评价研究奠定基础。
- 啤酒花 /
- 高效液相色谱 /
- 指纹图谱
Abstract: Objective To establish fingerprint of Hupulus lupulus by HPLC. Methods Diamonsil C₁₈ column (250 mm×4.6 mm, 5 μm) was used. The mobile phase consisted of acetonitrile and 0.1% phosphoric acid, in gradient elution mode, detection wavelength was 358 nm, column pressure at 10.2MPa, column temperature was 30℃, injection volume of 10 μl and flow rate was 1.0 ml/min. Results A common mode of HPLC fingerprint of Hupulus lupulus was established. 19 common peaks were calibrated, xanthohumol, cohumulone, humulone, adhumulone, colupulone, lupulone and adlupulone for identification. Among the 26 batches of Hupulus lupulus, 5 batches of herbs had similarities of less than or equal 0.9, which were all wild varieties. Conclusion The method was fast, effective, accurate and reliably to distinguish the cultivation and wildness Hupulus lupulus, which provided reference for quality control of Hupulus lupulus.
- Hupulus lupulus /
- HPLC /
- fingerprint

[1]	ZANOLI P, ZAVATTI M. Pharmacognostic and pharmacological profile of Humulus lupulus L.[J].J Ethnopharmacol,2008, 116(3):383-396.
[2]	国家中医药管理局《中华本草》编委会. 中华本草[M]. 上海:上海科学技术出版社, 2005.
[3]	HEYERICK A, VERVARCKE S, DEPYPERE H, et al. A first prospective,randomized, double-blind, placebo-controlled study on the use of a standardized hop extract to alleviate menopausal discomforts[J]. Maturitas,2006, 54, (2):164-175.
[4]	MILLIGAN S R, KALITA J C, POCOCK V, et al. The endocrine activities of 8-prenylnaringenin and related hop (Humulus lupulus L.) flavonoids[J].J Clin Endocrinol Metab, 2000, 85(12):4912-4915.
[5]	GERHAUSER C, ALT A, HEISS E, et al. Cancer chemopreventive activity of Xanthohumol, a natural product derived from hop[J]. Mol Cancer Ther, 2002, 1(11):959-969.

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啤酒花HPLC指纹图谱研究

doi: 10.3969/j.issn.1006-0111.2019.01.010

1. 上海师范大学生命与环境科学学院, 上海 200234;海军军医大学药学院, 上海 200433

2. 海军军医大学药学院, 上海 200433

3. 上海师范大学生命与环境科学学院, 上海 200234;海军军医大学药学院, 上海 200433;浙江中医药大学药学院, 浙江杭州 310053

基金项目: 国家自然科学基金（U1603283）

收稿日期: 2018-05-21
修回日期: 2018-10-08

关键词:

啤酒花 /

高效液相色谱 /

指纹图谱

摘要: 目的建立啤酒花HPLC指纹图谱。方法色谱柱：Diamonsil C₁₈柱（250 mm×4.6 mm，5 μm），流动相：乙腈-0.1%磷酸水，梯度洗脱，检测波长358 nm，柱压10.2 MPa，柱温30℃，进样量10 μl，流速1.0 ml/min。结果首次建立啤酒花HPLC指纹图谱共有模式，对其19个共有峰进行标定，并对其中的黄腐酚、类葎草酮、葎草酮、加葎草酮、类蛇麻酮、蛇麻酮、加蛇麻酮进行指认。26批药材中有5批药材相似度低于或等于0.9，而该5批均为野生品种。结论该方法高速有效，可准确地对啤酒花栽培种、野生种进行区分，为啤酒花的品质评价研究奠定基础。

English Abstract

Study on fingerprint of Hupulus lupulus by HPLC

1. College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China;School of Pharmacy, Naval Medical University, Shanghai 200433, China

2. School of Pharmacy, Naval Medical University, Shanghai 200433, China

3. College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China;School of Pharmacy, Naval Medical University, Shanghai 200433, China;College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310053, China

Received Date: 2018-05-21
Rev Recd Date: 2018-10-08

Keywords:

Abstract: Objective To establish fingerprint of Hupulus lupulus by HPLC. Methods Diamonsil C₁₈ column (250 mm×4.6 mm, 5 μm) was used. The mobile phase consisted of acetonitrile and 0.1% phosphoric acid, in gradient elution mode, detection wavelength was 358 nm, column pressure at 10.2MPa, column temperature was 30℃, injection volume of 10 μl and flow rate was 1.0 ml/min. Results A common mode of HPLC fingerprint of Hupulus lupulus was established. 19 common peaks were calibrated, xanthohumol, cohumulone, humulone, adhumulone, colupulone, lupulone and adlupulone for identification. Among the 26 batches of Hupulus lupulus, 5 batches of herbs had similarities of less than or equal 0.9, which were all wild varieties. Conclusion The method was fast, effective, accurate and reliably to distinguish the cultivation and wildness Hupulus lupulus, which provided reference for quality control of Hupulus lupulus.