葡萄籽提取物原花青素磷脂复合物抗氧化功能的实验研究

苗智如; 袁夏; 廖丽娜; 侯中海; 蒋国军

doi:10.3969/j.issn.1006-0111.2013.05.012

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葡萄籽提取物原花青素磷脂复合物抗氧化功能的实验研究

苗智如, 袁夏, 廖丽娜, 侯中海, 蒋国军

文章导航 > 药学实践与服务 > 2013 > 31(5): 362-365

苗智如, 袁夏, 廖丽娜, 侯中海, 蒋国军. 葡萄籽提取物原花青素磷脂复合物抗氧化功能的实验研究[J]. 药学实践与服务, 2013, 31(5): 362-365. doi: 10.3969/j.issn.1006-0111.2013.05.012

引用本文:

MIAO Zhi-ru, YUAN Xia, LIAO Li-na, HOU Zhong-hai, JIANG Guo-jun. Experimental study on anti-oxidative effect of proanthocyanidins phospholipid complex in grape seed[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(5): 362-365. doi: 10.3969/j.issn.1006-0111.2013.05.012

Citation:

MIAO Zhi-ru, YUAN Xia, LIAO Li-na, HOU Zhong-hai, JIANG Guo-jun. Experimental study on anti-oxidative effect of proanthocyanidins phospholipid complex in grape seed[J]. Journal of Pharmaceutical Practice and Service, 2013, 31(5): 362-365. doi: 10.3969/j.issn.1006-0111.2013.05.012

葡萄籽提取物原花青素磷脂复合物抗氧化功能的实验研究

doi: 10.3969/j.issn.1006-0111.2013.05.012

1.
杭州市萧山区第一人民医院药剂科, 浙江杭州 311200
2.
浙江萧山医院药剂科, 浙江杭州 311201

基金项目: 杭州市科学技术局医疗卫生科研项目(20100633B26);杭州市萧山区科学技术局重点科研项目(2010225).

Experimental study on anti-oxidative effect of proanthocyanidins phospholipid complex in grape seed

1.
Department of Pharmacy, the First People s Hospital of Xiaoshan, Hangzhou 311200, China
2.
Department of Pharmacy, Zhejiang Xiaoshan Hospital, Hangzhou 311201, China

摘要: 目的探讨葡萄籽提取物原花青素磷脂复合物的抗氧化作用。方法取大鼠80只,随机分为8组:空白对照组、模型组、原花青素组、大豆磷脂组、维生素C组、原花青素磷脂复合物低、中、高剂量组。每天灌胃1次,连续灌胃30 d。然后用50%乙醇给大鼠一次性灌胃,造成乙醇氧化损伤模型。通过测定大鼠体重,血清和肝组织中丙二醛含量、蛋白质羰基含量、超氧化物歧化酶和谷胱甘肽过氧化物酶活性及还原性谷胱甘肽含量的变化,观察葡萄籽提取物原花青素磷脂复合物的抗氧化作用。结果原花青素磷脂复合物各剂量组的大鼠体重与模型组及空白对照组比较,均无统计学差异(P>0.05)。原花青素磷脂复合物低剂量组大鼠血清和肝组织MDA含量明显低于模型组(P<0.05),血清和肝组织GSH-PX活性明显高于模型组(P<0.05)。与模型组比较,原花青素磷脂复合物中剂量组和高剂量组大鼠血清和肝组织中SOD、GSH-PX活性及GSH含量较模型组显著升高(P<0.05,P<0.01),MDA和蛋白质羰基含量明显降低(P<0.05,P<0.01)。结论葡萄籽提取物原花青素磷脂复合物具有显著地抗氧化作用。
- 葡萄籽提取物 /
- 原花青素 /
- 大豆磷脂 /
- 抗氧化作用
Abstract: Objective To study the antioxidant effects of grape seed proanthocyanidins phospholipid complex(GSPPC). Methods Eighty rats were randomly divided into eight groups: normal control, model control, Proanthocyanidins, Soybean phospholipids, Vitamin C and low dose of GSPPC, moderate dose of GSPPC, and high dose of GSPPC. Drugs were given orally to rats once a day for 30 days. And then, 50% ethanol was given to rats once by oral gavages to make the ethanol oxidative damage model at the end of experiment. The body weight of rats was measured. The contents of MDA, Protein carbonyl (PC), SOD, GSH and GSH-PX in hepatic tissue and blood serum were detected. Results The results showed there were no significant differences in the body weight among the three GSPPC groups, normal control and model control(P>0.05). Compared with the model control group, the content of MDA in low dosage of the GSPPC group was decreased in blood serum and hepatic tissue(P<0.05), and the content of GSH-PX was increased in blood serum and hepatic tissue (P<0.05).Compared with the model control group, the contents of GSH, SOD and GSH-PX were increased in blood serum and hepatic tissue(P<0.05, P<0.01), the contents of MDA and PC in middle and high dosage of the GSPPC group were decreased in hepatic tissue and blood serum(P<0.05, P<0.01). Conclusion The GSPPC showed significantly antioxidant effect.
- Proanthocyanidins /
- Soybean phospholipids /
- Antioxidant effect /
- MDA

[1]	周坦洋,罗芙蓉,白彬.葡萄籽原花青素生物药理活性的研究进展[J].哈尔滨医科大学学报,2012,46(1):94.
[2]	剧红梅,曲梓怡,王召令,等.原花青素的抗氧化作用[J].中国组织工程研究与临床康复,2007,11(16):3155.
[3]	黄进,杨丹丹,郝书颖,等.大豆磷脂对肉仔鸡抗氧化性能影响的研究[J].饲料工业,2012,33(7):15.
[4]	凌沛学,汤漩,王凤山,等.药物与磷脂复合物研究近况[J]. 中国药学杂志.2005,40(6):401.
[5]	国家食品药品监督管理局.抗氧化功能评价方法[S].2012:1.
[6]	左巨波,尚京川.中药磷脂复合物的研究进展[J]. 中国药房.2007,18(27):2149.
[7]	戴伟,尹晓晨.蜂胶对乙醇所致化学性肝损伤的保护作用研究[J].实用预防医学,2010,17(6):1207.
[8]	段丽菊,刘英帅,朱燕,等.DNPH比色法:一种简单的蛋白质羰基含量测定方法[J].毒理学杂志,2005,19(4):320.
[9]	毕玲,傅柏平.葡萄籽原花青素提取物的研究进展[J].中国新药杂志,2008,17(17):1478.

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葡萄籽提取物原花青素磷脂复合物抗氧化功能的实验研究

doi: 10.3969/j.issn.1006-0111.2013.05.012

1. 杭州市萧山区第一人民医院药剂科, 浙江杭州 311200

2. 浙江萧山医院药剂科, 浙江杭州 311201

基金项目: 杭州市科学技术局医疗卫生科研项目(20100633B26);杭州市萧山区科学技术局重点科研项目(2010225).

收稿日期: 2013-04-17
修回日期: 2013-06-28

关键词:

摘要: 目的探讨葡萄籽提取物原花青素磷脂复合物的抗氧化作用。方法取大鼠80只,随机分为8组:空白对照组、模型组、原花青素组、大豆磷脂组、维生素C组、原花青素磷脂复合物低、中、高剂量组。每天灌胃1次,连续灌胃30 d。然后用50%乙醇给大鼠一次性灌胃,造成乙醇氧化损伤模型。通过测定大鼠体重,血清和肝组织中丙二醛含量、蛋白质羰基含量、超氧化物歧化酶和谷胱甘肽过氧化物酶活性及还原性谷胱甘肽含量的变化,观察葡萄籽提取物原花青素磷脂复合物的抗氧化作用。结果原花青素磷脂复合物各剂量组的大鼠体重与模型组及空白对照组比较,均无统计学差异(P>0.05)。原花青素磷脂复合物低剂量组大鼠血清和肝组织MDA含量明显低于模型组(P<0.05),血清和肝组织GSH-PX活性明显高于模型组(P<0.05)。与模型组比较,原花青素磷脂复合物中剂量组和高剂量组大鼠血清和肝组织中SOD、GSH-PX活性及GSH含量较模型组显著升高(P<0.05,P<0.01),MDA和蛋白质羰基含量明显降低(P<0.05,P<0.01)。结论葡萄籽提取物原花青素磷脂复合物具有显著地抗氧化作用。

English Abstract

Experimental study on anti-oxidative effect of proanthocyanidins phospholipid complex in grape seed

1. Department of Pharmacy, the First People s Hospital of Xiaoshan, Hangzhou 311200, China

2. Department of Pharmacy, Zhejiang Xiaoshan Hospital, Hangzhou 311201, China

Received Date: 2013-04-17
Rev Recd Date: 2013-06-28

Keywords:

Abstract: Objective To study the antioxidant effects of grape seed proanthocyanidins phospholipid complex(GSPPC). Methods Eighty rats were randomly divided into eight groups: normal control, model control, Proanthocyanidins, Soybean phospholipids, Vitamin C and low dose of GSPPC, moderate dose of GSPPC, and high dose of GSPPC. Drugs were given orally to rats once a day for 30 days. And then, 50% ethanol was given to rats once by oral gavages to make the ethanol oxidative damage model at the end of experiment. The body weight of rats was measured. The contents of MDA, Protein carbonyl (PC), SOD, GSH and GSH-PX in hepatic tissue and blood serum were detected. Results The results showed there were no significant differences in the body weight among the three GSPPC groups, normal control and model control(P>0.05). Compared with the model control group, the content of MDA in low dosage of the GSPPC group was decreased in blood serum and hepatic tissue(P<0.05), and the content of GSH-PX was increased in blood serum and hepatic tissue (P<0.05).Compared with the model control group, the contents of GSH, SOD and GSH-PX were increased in blood serum and hepatic tissue(P<0.05, P<0.01), the contents of MDA and PC in middle and high dosage of the GSPPC group were decreased in hepatic tissue and blood serum(P<0.05, P<0.01). Conclusion The GSPPC showed significantly antioxidant effect.

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葡萄籽提取物原花青素磷脂复合物抗氧化功能的实验研究

doi: 10.3969/j.issn.1006-0111.2013.05.012

Experimental study on anti-oxidative effect of proanthocyanidins phospholipid complex in grape seed

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葡萄籽提取物原花青素磷脂复合物抗氧化功能的实验研究

doi: 10.3969/j.issn.1006-0111.2013.05.012

1. 杭州市萧山区第一人民医院药剂科, 浙江 杭州 311200 2. 浙江萧山医院药剂科, 浙江 杭州 311201

English Abstract

Experimental study on anti-oxidative effect of proanthocyanidins phospholipid complex in grape seed

1. Department of Pharmacy, the First People s Hospital of Xiaoshan, Hangzhou 311200, China 2. Department of Pharmacy, Zhejiang Xiaoshan Hospital, Hangzhou 311201, China

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目录

1. 杭州市萧山区第一人民医院药剂科, 浙江杭州 311200

2. 浙江萧山医院药剂科, 浙江杭州 311201

1. Department of Pharmacy, the First People s Hospital of Xiaoshan, Hangzhou 311200, China

2. Department of Pharmacy, Zhejiang Xiaoshan Hospital, Hangzhou 311201, China