HPLC法测定土鳖虫中尿嘧啶和尿囊素的含量
Determination of auantion and uracil in Eupolyphaga seu Steleophaga by HPLC
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摘要: 目的: 建立土鳖虫药材中尿嘧啶和尿囊素的含量测定方法。 方法: 采用高效液相色谱法,色谱柱为AlltimaNH2(150 mm×4.6 mm,5μm);流动相为乙腈-水(90:10);流速为1.0 ml/min,检测波长205 nm;柱温35℃。 结果: 尿囊素和尿嘧啶的线性范围分别是:0.404-3.23μg(r=0.999 5,n=5),0.820-6>.56μg(r=0.999 5,n=5)。平均回收率分别为98.8%、99.0%,RSD分别为0.96%、0.79%(n=6)。 结论: 该方法简便、准确,可作为土鳖虫药材的质量控制方法。Abstract: Objective To establish a method for determination of uracil and auantion in Eupolyphaga seu Steleophaga by HPLC. Methods The HPLC separation was performed on Alltima NH2(150 mm×4.6 mm,5 μm)column with a mixture of acetonitrile-water(9010)as the mobile phase,at a flow rate of 1.0 ml/min.Detection wavelength was at 205 nm,and column temperature was at 35 ℃. Results The linear response of Auantion and Uracil range from 0.404~3.23 μg(r=0.999 5,n=5)and 0.820~6.56 μg(r=0.999 5,n=5);the average recovery was 98.8% with RSD 0.96% and 99.0% with RSD 0.79% respectively(n=6). Conclusion The method is simple,accurate and suitable for the determination of auantion and uracil in Eupolyphaga seu Steleophaga.
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Key words:
- Eupolyphaga seu Steleophaga /
- auantion /
- uracil /
- HPLC
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