枳壳总黄酮提取工艺与含量测定研究
Extraction and content determination of total flavonoid from Fructus Aurantii
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摘要: 目的: 研究枳壳总黄酮提取工艺与含量测定方法。 方法: 采用正交实验,筛选枳壳总黄酮最佳提取工艺条件,用紫外分光光度法测定总黄酮的含量。 结果: 枳壳总黄酮的最佳提取条件为:在温度70℃,pH3.0条件下,加15倍的Ca（OH）2饱和溶液,浸提2 h/次,提取3次,以柚皮苷为对照测定总黄酮含量,柚皮苷线性范围为2.59241.472μg/mL,r=0.9999。 结论: 所建方法快速、简便,可用于枳壳药材质量控制。Abstract: Objective: To study the extraction process and content determination of total flavonoids from Fructus Aurantii. Methods: The optimum extraction conditions of total flavonoids from Fructus Aurantii were screened with orthogonal test and the content of total flavonoid with standard hesperidin was determined by UV spectrophotometry. Results: The best extraction conditions were as follow:adding 15 times amount of Ca(OH)2,pH 3.0 and at 70 ℃ for 3 times in all,2 hours each time.The linear range of naringin was 2.592～41.472μg/mL r=0.999 9. Conclusion: The methods are simple,fast and can be used to control the quality of Fructus Aurantii.
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Key words:
- Fructus Aurantii /
- total flavonoid /
- extraction
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