2002 Vol. 20, No. 5
Display Method:
2002, (5): 277-280.
Abstract:
OBJECTIVE To prepare Jiangzhi Baogan granules, formulate quality standard and observe its clinical efficacy. METHODS To adopt the technique of water extraction to make the granules of pure chinese traditional medicine, the identification, extractive determination and clinical application were finished. RESULTS The prescription was reasonable, the preparation technology was stable and the preparation was completely conformed to the quality standard. Among 100 cases observed, total clinical effective rate was 92%.C0NCLUSION The granules had good efficacy for hyperlipemia disease and was worth to extend to apply in clinic.
OBJECTIVE To prepare Jiangzhi Baogan granules, formulate quality standard and observe its clinical efficacy. METHODS To adopt the technique of water extraction to make the granules of pure chinese traditional medicine, the identification, extractive determination and clinical application were finished. RESULTS The prescription was reasonable, the preparation technology was stable and the preparation was completely conformed to the quality standard. Among 100 cases observed, total clinical effective rate was 92%.C0NCLUSION The granules had good efficacy for hyperlipemia disease and was worth to extend to apply in clinic.
2002, (5): 280-282.
Abstract:
OBJECTIVE To study the influence of microwave technique on the amount of baicalin infused from Scutellaria Baiacalensis.METHODS With some different infusing methods, powder radius of Chinese medicinal material, diffused time, output power microwave orthogonal test was carried out. Choose the best infusing test design. The amount of baicalin in the Scutellaria baiacalensis infusion was determined by UV.RESULTS Influence of microw ave technique on the amount of baicalin is superior to the general decocting method.CONCLUSION Application of microwave technique in the Chinese medicinal material infusion is a new method which is to save time and worth of spreading out.The amount of baicalin diffused from half amount Scutellaria baiacalensis is larger than total amount of its prepared herbal medicine. The result is coincidence with the traditional Chinese medical theory-"amount of Zhu San(powder decoction)is reduced by half."
OBJECTIVE To study the influence of microwave technique on the amount of baicalin infused from Scutellaria Baiacalensis.METHODS With some different infusing methods, powder radius of Chinese medicinal material, diffused time, output power microwave orthogonal test was carried out. Choose the best infusing test design. The amount of baicalin in the Scutellaria baiacalensis infusion was determined by UV.RESULTS Influence of microw ave technique on the amount of baicalin is superior to the general decocting method.CONCLUSION Application of microwave technique in the Chinese medicinal material infusion is a new method which is to save time and worth of spreading out.The amount of baicalin diffused from half amount Scutellaria baiacalensis is larger than total amount of its prepared herbal medicine. The result is coincidence with the traditional Chinese medical theory-"amount of Zhu San(powder decoction)is reduced by half."
2002, (5): 285-286.
Abstract:
OBJECTIVE To establish UV method for the determination of content in tetracaine hydrochloride eye drops.METHODS UV spectrophotometry was used to determine the tetracaine hydrochloride eye drops.The sample was determined at 312nm. RESULTS The standanl curver was good linear within the range of 3.5-10.5μg·ml-1,(r=0.9999)。The average recovery was 100.09%,RSD was 1.06%(n=3).CONCLUSIGN The method is simple, reliable, accurate and can be used for the determination of the content of tetracaine hydrochloride eye drops.
OBJECTIVE To establish UV method for the determination of content in tetracaine hydrochloride eye drops.METHODS UV spectrophotometry was used to determine the tetracaine hydrochloride eye drops.The sample was determined at 312nm. RESULTS The standanl curver was good linear within the range of 3.5-10.5μg·ml-1,(r=0.9999)。The average recovery was 100.09%,RSD was 1.06%(n=3).CONCLUSIGN The method is simple, reliable, accurate and can be used for the determination of the content of tetracaine hydrochloride eye drops.
2002, (5): 286-288.
Abstract:
OBJECTIVE To establish a reversed-phase HPLC method for determination of contents of baicalin in Qinlingzi vagina perfusate.METHODS RP-HPLC analysis was carried out by using spheridorb C18 column and methanol water-phosphoie(51:49:0.1) as the mobile the detection wavelength was 278nm. The concentratinn of baiealin was quantified by external standard. RESULTS The method was linear with the range of 5.0-80.0μg·ml-1,the regression equation was A=32365.56C-1997.75, r=0.9999(n=5),the average recovery was 97.85%(n=5) and the RSD was 0.86%(n=6).CONCLUSION The method is convenient, rapid and reproducible, it may be used for the quantitative determination of baicalin in Qinlingzi vagina perfusate.
OBJECTIVE To establish a reversed-phase HPLC method for determination of contents of baicalin in Qinlingzi vagina perfusate.METHODS RP-HPLC analysis was carried out by using spheridorb C18 column and methanol water-phosphoie(51:49:0.1) as the mobile the detection wavelength was 278nm. The concentratinn of baiealin was quantified by external standard. RESULTS The method was linear with the range of 5.0-80.0μg·ml-1,the regression equation was A=32365.56C-1997.75, r=0.9999(n=5),the average recovery was 97.85%(n=5) and the RSD was 0.86%(n=6).CONCLUSION The method is convenient, rapid and reproducible, it may be used for the quantitative determination of baicalin in Qinlingzi vagina perfusate.
2002, (5): 289-290.
Abstract:
OBJECTIVE To determine the contents of diethylstilbestrol by capillary zone electroporesis(CZE).METHODS Using cinnamon acid as the internal standard(IS),the electrophoretic separation was achieved with 25 mmol·L-1borate(pH=9.18) as the running buffer and a voltage of 24 kV was applied to the 45cm×50μm(i.d) capillary.The analysis were introduced into capillary by siphon(1s) and determined with on-column monitoring at 214nm. RESULTS The determination could be completed w ithin 4 min and the minimum concentration of detection was 10μg·ml-1.The analytical results of diethylstilbestrol samples demonstraoted a good linear relationship within the 105.7-528.5μg·ml-1.The relative standard deviation (RSD)of intra-day was less than 1.61% and that of inter-day was less 3.76%.The present recoveries were bettween 97.91%-101.58%.CONCLUSION This method is accurate, simple, sensitive and reliable, which can be applied to the determination of diethylstilbestrol content.
OBJECTIVE To determine the contents of diethylstilbestrol by capillary zone electroporesis(CZE).METHODS Using cinnamon acid as the internal standard(IS),the electrophoretic separation was achieved with 25 mmol·L-1borate(pH=9.18) as the running buffer and a voltage of 24 kV was applied to the 45cm×50μm(i.d) capillary.The analysis were introduced into capillary by siphon(1s) and determined with on-column monitoring at 214nm. RESULTS The determination could be completed w ithin 4 min and the minimum concentration of detection was 10μg·ml-1.The analytical results of diethylstilbestrol samples demonstraoted a good linear relationship within the 105.7-528.5μg·ml-1.The relative standard deviation (RSD)of intra-day was less than 1.61% and that of inter-day was less 3.76%.The present recoveries were bettween 97.91%-101.58%.CONCLUSION This method is accurate, simple, sensitive and reliable, which can be applied to the determination of diethylstilbestrol content.
2002, (5): 291-293.
Abstract:
OBJECTIVE To determine the contents of indigotin and indirubin in Indigo Naturalis and the extract of Indigo Naturalis with supercritical fluid extraction. METHODS The indirubin content was determined with sulfonate reaction-first order derivative-UV spectrophotometry; the indigotin content was determined with sulfonated reaotion-UV spectrophotometry.RESULTS The average recovery rate of indirubin was 101.80%,RSD=0.75%,and the average recovery rate of indigotin was 101.06%,RSD=1.27%.CONCLUSTION This method is simple and repeatable, and can be used as the quality control for method for the Indigo Naturalis and the extract of Indigo Naturalis with supercritical fluid extraction.
OBJECTIVE To determine the contents of indigotin and indirubin in Indigo Naturalis and the extract of Indigo Naturalis with supercritical fluid extraction. METHODS The indirubin content was determined with sulfonate reaction-first order derivative-UV spectrophotometry; the indigotin content was determined with sulfonated reaotion-UV spectrophotometry.RESULTS The average recovery rate of indirubin was 101.80%,RSD=0.75%,and the average recovery rate of indigotin was 101.06%,RSD=1.27%.CONCLUSTION This method is simple and repeatable, and can be used as the quality control for method for the Indigo Naturalis and the extract of Indigo Naturalis with supercritical fluid extraction.
2002, (5): 293-295.
Abstract:
OBJECTIVE To develop a method for assay of roxithromycin in electron medicinal membrane of roxithromycin. METHODS To use a HPLC method by YWG C18 column. The mobile phase was a mixture of acetonitrile-methano-l0.5% ammonium acetate(100:80:60),with the detection wavelength at 235 nm.RESULTS Precision and stability were fine. The linear correlation was observed from the concentrations of 0.3 to 1.7mg·ml-1(r=0.9998).The average recovery was 99.23%.Resolution bettween roxithromycin and other peaks met the requirements.CONCLUSION The method is convenient, selective and reproducible.
OBJECTIVE To develop a method for assay of roxithromycin in electron medicinal membrane of roxithromycin. METHODS To use a HPLC method by YWG C18 column. The mobile phase was a mixture of acetonitrile-methano-l0.5% ammonium acetate(100:80:60),with the detection wavelength at 235 nm.RESULTS Precision and stability were fine. The linear correlation was observed from the concentrations of 0.3 to 1.7mg·ml-1(r=0.9998).The average recovery was 99.23%.Resolution bettween roxithromycin and other peaks met the requirements.CONCLUSION The method is convenient, selective and reproducible.
2002, (5): 295-297.
Abstract:
OBJECTIVE To develop a method for determinating the contents of diphenhydramine hydrochloride and ephedrine hydrochloride in nasal drops.METHODS The dual-wavelength spectrophotometric method was used for eliminating matual interference.RESULTS The method was linear and reproducible.The average recovery was 100.29%, RSD=0.82(n=9) for diphenhydramine hydrochloride and 99.92%, RSD=0.90(n=9) for ephedrine hydrochloride respectively.CONCLUSION The method was simple,rapid and accurate.
OBJECTIVE To develop a method for determinating the contents of diphenhydramine hydrochloride and ephedrine hydrochloride in nasal drops.METHODS The dual-wavelength spectrophotometric method was used for eliminating matual interference.RESULTS The method was linear and reproducible.The average recovery was 100.29%, RSD=0.82(n=9) for diphenhydramine hydrochloride and 99.92%, RSD=0.90(n=9) for ephedrine hydrochloride respectively.CONCLUSION The method was simple,rapid and accurate.
2002, (5): 297-300.
Abstract:
OBJECTIVE To establish a method to determinate the quantity of anthraquinones in Zhuyufuzheng capsule.METHODS Anthraquinones in Zhuyufuzheng capsules was determined by improved colorimetry.RESULTS This method was simple,quick and reproducible(RSD=1.58%, n=5).The average recovery was 99.86%.By this method,the quality of Zhuyufuzheng capsules can be controlled effectively.CONCLUSION The improved colorimetry can be used to determinate the quantity of anthraquinones in Zhuyufuzheng capsules.This method can be used for the quantitative analysis of other Chinese traditional medicines which contained anthraquinones as well.
OBJECTIVE To establish a method to determinate the quantity of anthraquinones in Zhuyufuzheng capsule.METHODS Anthraquinones in Zhuyufuzheng capsules was determined by improved colorimetry.RESULTS This method was simple,quick and reproducible(RSD=1.58%, n=5).The average recovery was 99.86%.By this method,the quality of Zhuyufuzheng capsules can be controlled effectively.CONCLUSION The improved colorimetry can be used to determinate the quantity of anthraquinones in Zhuyufuzheng capsules.This method can be used for the quantitative analysis of other Chinese traditional medicines which contained anthraquinones as well.
2002, (5): 300-302.
Abstract:
OBJECTIVE To establish a method to determine the content of total astragalosides in Fuzhengpingxiao capsules, and primary research about the extraction has been done with this method. METHODS Three different methods were used to prepare the crude extractive liquid, which were extracted by pure water,60% alcohol and deposited by alcohol after extracted by pure water. The crude extractive liquid was extracted by n-butanol saturated by water. Colorimetric analysis was used to determine the content of total astragalosides. RESULTS The mean content of total astagalosides extracted by three different methods were 0.26%,0.34% and 0.16%,respectively. The amount of the total astragalosides extracted by 60% alcohol was the highest. CONCLUSION The method can be used to control the quality of Fuzhengpingxiao capsules.
OBJECTIVE To establish a method to determine the content of total astragalosides in Fuzhengpingxiao capsules, and primary research about the extraction has been done with this method. METHODS Three different methods were used to prepare the crude extractive liquid, which were extracted by pure water,60% alcohol and deposited by alcohol after extracted by pure water. The crude extractive liquid was extracted by n-butanol saturated by water. Colorimetric analysis was used to determine the content of total astragalosides. RESULTS The mean content of total astagalosides extracted by three different methods were 0.26%,0.34% and 0.16%,respectively. The amount of the total astragalosides extracted by 60% alcohol was the highest. CONCLUSION The method can be used to control the quality of Fuzhengpingxiao capsules.
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