Determination of diethylstilbestrol in diethylstilbestrol tablet by capillary zone electrophoresis

Key words: 
• capillary zone electrophoresis /
• diethylstilbestrol /
• cinnamon acid

Abstract: OBJECTIVE To determine the contents of diethylstilbestrol by capillary zone electroporesis(CZE).METHODS Using cinnamon acid as the internal standard(IS),the electrophoretic separation was achieved with 25 mmol·L^-1borate(pH=9.18) as the running buffer and a voltage of 24 kV was applied to the 45cm×50μm(i.d) capillary.The analysis were introduced into capillary by siphon(1s) and determined with on-column monitoring at 214nm. RESULTS The determination could be completed w ithin 4 min and the minimum concentration of detection was 10μg·ml^-1.The analytical results of diethylstilbestrol samples demonstraoted a good linear relationship within the 105.7-528.5μg·ml^-1.The relative standard deviation (RSD)of intra-day was less than 1.61% and that of inter-day was less 3.76%.The present recoveries were bettween 97.91%-101.58%.CONCLUSION This method is accurate, simple, sensitive and reliable, which can be applied to the determination of diethylstilbestrol content.