The assay of three triterpenoid saponin extracted from Bessia calthaefolia simultaneously by HPLC-ELSD and their antioxidant effects in vitro
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摘要: 目的 建立同时测定铁破锣中3种三萜皂苷BeesiosideⅠ、Ⅱ、Ⅲ含量的方法，并研究铁破锣中三萜皂苷的体外抗氧化性。 方法 运用反相高效液相色谱-蒸发光散射检测法（HPLC-ELSD）对铁破锣中三萜皂苷进行含量测定，并对所用含量测定方法进行方法学考察；同时，对铁破锣中三萜皂苷清除自由基的能力和总的抗氧化能力进行体外试验。 结果 HPLC-ELSD方法可同时测定BeesiosideⅠ、Ⅱ、Ⅲ的含量，且该方法线性关系良好、精密度、重复性、稳定性均好，回收率高；铁破锣中三萜皂苷能够有效清除ABTS+、DPPH·和羟基自由基，并且具有很好的抗氧化能力。 结论 HPLC-ELSD方法简便、快速、准确、重现性好，可作为铁破锣中三萜皂苷（BeesiosideⅠ、Ⅱ、Ⅲ）的含量测定及质量控制标准方法，体外试验表明铁破锣中三萜皂苷具有良好的自由基清除和抗氧化能力。
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关键词:
- 铁破锣 /
- 三萜皂苷 /
- 高效液相色谱-蒸发光散射法 /
- 抗氧化
Abstract: Objective To establish a method for simultaneous determination of three triterpenoid saponins (BeesiosideⅠ、Ⅱ、Ⅲ),and investigate antioxidant effects of triterpenoid saponin extracted from Bessia calthaefolia. Methods The content assay of triterpenoid saponin extracted from Bessia calthaefolia was conducted by high performance liquid chromatography method with evaporative light-scattering detector (HPLC-ELSD).The methodology validation was performed to confirm the content determination.The free radicals scavenging activities and antioxidant effects of the triterpenoid saponin were evaluated in vitro. Results The HPLC-ELSD method can simultaneously determine the content of Beesioside I,Ⅱ and Ⅲ.It has good linearity,precision,repeatability and stability with high recovery.The triterpenoid saponins showed great activity on scavenging ABTS+、DPPH·and OH free radicals and antioxidant effects. Conclusion The HPLC-ELSD method has good specificity,stability,repeatability and accuracy.This method can be used for content determination and quality standard of three triterpenoid saponins (BeesiosideⅠ、Ⅱ、Ⅲ) from Bessia calthaefolia.The triterpenoid saponins exhibited free radicals scavenging effects and antioxidant effects.-
Key words:
- Beesia calthaefolia /
- triterpenoid saponin /
- HPLC-ELSD method /
- antioxidant effects
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