在线样品预处理限进色谱测定人血清中多奈哌齐浓度

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在线样品预处理限进色谱测定人血清中多奈哌齐浓度

马骏, 樊鹏程, 任俊, 贾正平

文章导航 > 药学实践与服务 > 2011 > 29(2): 122-124

马骏, 樊鹏程, 任俊, 贾正平. 在线样品预处理限进色谱测定人血清中多奈哌齐浓度[J]. 药学实践与服务, 2011, 29(2): 122-124.

引用本文:

马骏, 樊鹏程, 任俊, 贾正平. 在线样品预处理限进色谱测定人血清中多奈哌齐浓度[J]. 药学实践与服务, 2011, 29(2): 122-124.

MA Jun, FAN Peng-cheng, REN Jun, JIA Zheng-ping. Detection of donepezil from serum by on-line sample preparation RAM-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(2): 122-124.

Citation:

MA Jun, FAN Peng-cheng, REN Jun, JIA Zheng-ping. Detection of donepezil from serum by on-line sample preparation RAM-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(2): 122-124.

在线样品预处理限进色谱测定人血清中多奈哌齐浓度

兰州军区兰州总医院药剂科,甘肃兰州 730050

Detection of donepezil from serum by on-line sample preparation RAM-HPLC

Department of Pharmacy, General Hospital of Lanzhou Command, Lanzhou 730050,China

摘要: 目的通过在线样品预处理限进色谱柱切换技术建立一种测定人体血清中多奈哌齐血药浓度的高效液相色谱法。方法样品预处理采用LiChroCART (25 mm × 4 mm,25 μm) 限进预柱,分析柱为Microsorb-MV C₁₈柱 (150 mm × 4.6 mm,4 μm ),以水-乙腈 (991, v/v) 为血浆蛋白冲洗流动相,流速2 ml/min,0.02 M 磷酸缓冲液-乙腈-三乙胺 (65350.1, v/v/v) 为分析流动相,流速1 ml/min,检测波长315 nm。结果本方法线性范围为0.02~1.0 μg/ml,r=0.999 5,日内、日间精密度RSD小于7%,回收率为87.9%~91.1%。结论该方法准确、稳定;灵敏度高,适用于多奈哌齐药物动力学及生物利用度的研究。
- 限进填料 /
- 在线样品预处理 /
- 多奈哌齐 /
- 柱切换
Abstract: Objective To establish a sample preparation restricted access material (RAM) HPLC method for the quantification of donepezil in human serum. Method The sample was prepocessed by LiChrospher^ ADS C₁₈ (25 mm × 4 mm,25 μm) as pre-column and analyzed by Microsorb-MV C₁₈ (150 mm × 4.6 mm,4 μm ) as analytical column. Acetonitrile to water (991, v/v) was flushing mobile phase with flow rate of 2 ml/min. The mixture of phosphate buffer (pH 4.6, 0.02 M), acetonitril and triethylamine (65350.1, v/v/v) was analytical mobile phase with flow rate of 1 ml/min. The detection wave was 315 nm. Results The linear range was from 0.02 to 1.0 μg/ml (r=0.999 5). The RSDs of intra-day and inter-day were less than 7%, the recoveries were between 87.9%~91.1%. Conclusion The method is accurate, stable and sensitive which could be used to study the pharmacodynamics and bioavailabilty of donepezil.
- restricted access material /
- on-line sample preparation /
- donepezil /
- column-switching

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在线样品预处理限进色谱测定人血清中多奈哌齐浓度

兰州军区兰州总医院药剂科,甘肃兰州 730050

收稿日期: 2009-12-10
修回日期: 2010-10-02

关键词:

在线样品预处理 /

摘要: 目的通过在线样品预处理限进色谱柱切换技术建立一种测定人体血清中多奈哌齐血药浓度的高效液相色谱法。方法样品预处理采用LiChroCART (25 mm × 4 mm,25 μm) 限进预柱,分析柱为Microsorb-MV C₁₈柱 (150 mm × 4.6 mm,4 μm ),以水-乙腈 (991, v/v) 为血浆蛋白冲洗流动相,流速2 ml/min,0.02 M 磷酸缓冲液-乙腈-三乙胺 (65350.1, v/v/v) 为分析流动相,流速1 ml/min,检测波长315 nm。结果本方法线性范围为0.02~1.0 μg/ml,r=0.999 5,日内、日间精密度RSD小于7%,回收率为87.9%~91.1%。结论该方法准确、稳定;灵敏度高,适用于多奈哌齐药物动力学及生物利用度的研究。

English Abstract

马骏, 樊鹏程, 任俊, 贾正平. 在线样品预处理限进色谱测定人血清中多奈哌齐浓度[J]. 药学实践与服务, 2011, 29(2): 122-124.

引用本文:

马骏, 樊鹏程, 任俊, 贾正平. 在线样品预处理限进色谱测定人血清中多奈哌齐浓度[J]. 药学实践与服务, 2011, 29(2): 122-124.

MA Jun, FAN Peng-cheng, REN Jun, JIA Zheng-ping. Detection of donepezil from serum by on-line sample preparation RAM-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(2): 122-124.

Citation:

MA Jun, FAN Peng-cheng, REN Jun, JIA Zheng-ping. Detection of donepezil from serum by on-line sample preparation RAM-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(2): 122-124.

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