原料纯度对白及多糖疏水改性反应取代度的影响

赵雪竹; 刘洋; 王艳萍; 刘福强; 张琦; 刘丹; 赵楠; 韩丹; 赵庆兰

doi:10.3969/j.issn.1006-0111.2015.03.008

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原料纯度对白及多糖疏水改性反应取代度的影响

赵雪竹, 刘洋, 王艳萍, 刘福强, 张琦, 刘丹, 赵楠, 韩丹, 赵庆兰

文章导航 > 药学实践与服务 > 2015 > 33(3): 221-225

赵雪竹, 刘洋, 王艳萍, 刘福强, 张琦, 刘丹, 赵楠, 韩丹, 赵庆兰. 原料纯度对白及多糖疏水改性反应取代度的影响[J]. 药学实践与服务, 2015, 33(3): 221-225. doi: 10.3969/j.issn.1006-0111.2015.03.008

引用本文:

ZHAO Xuezhu, LIU Yang, WANG Yanping, LIU Fuqiang, ZHANG Qi, LIU Dan, ZHAO Nan, HAN Dan, ZHAO Qinglan. Effect of raw material purity on the degree of substitution of hydrophobically modified Bletilla striata polysaccharide[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(3): 221-225. doi: 10.3969/j.issn.1006-0111.2015.03.008

Citation:

ZHAO Xuezhu, LIU Yang, WANG Yanping, LIU Fuqiang, ZHANG Qi, LIU Dan, ZHAO Nan, HAN Dan, ZHAO Qinglan. Effect of raw material purity on the degree of substitution of hydrophobically modified Bletilla striata polysaccharide[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(3): 221-225. doi: 10.3969/j.issn.1006-0111.2015.03.008

原料纯度对白及多糖疏水改性反应取代度的影响

doi: 10.3969/j.issn.1006-0111.2015.03.008

1.
延边大学药学院, 吉林延吉 133000;解放军208医院, 吉林长春 130062
2.
沈阳军区长春干休所, 吉林长春 130000
3.
解放军208医院, 吉林长春 130062

基金项目: 国家自然科学基金项目(81173559)

Effect of raw material purity on the degree of substitution of hydrophobically modified Bletilla striata polysaccharide

1.
School of Pharmacy, Yanbian University, Yanji 133000, China;No. 208 Hospital of PLA, Changchun 130062, China
2.
Changchun Sanatorium for Retired Cadres, Shenyang Millitary Region, Changchun 130000, China
3.
No. 208 Hospital of PLA, Changchun 130062, China

摘要: 目的通过纯化反应原料之一胆甾醇琥珀酸酯(CHS),提高疏水改性白及多糖的胆甾醇基取代度。方法利用重结晶法纯化CHS,并采用差示量热扫描法(DSC)及X射线衍射法(XRD)对CHS纯品进行表征;用高效液相色谱法(HPLC)测定纯化前后产品中游离胆甾醇的含量;¹H-NMR法测定CHS纯化前后改性白及多糖的胆甾醇基取代度。结果重结晶后的CHS纯度较高,具有晶体性质,可明显提高改性白及多糖的胆甾醇基取代度。结论纯化方法简便、可行,为提高改性白及多糖胆甾醇基取代度提供研究基础。
- 原料纯度 /
- 胆甾醇琥珀酸酯 /
- 取代度
Abstract: Objective To improve the degree of substitution of the cholesteryl by purifying cholesteryl succinyl ester(CHS), one of the raw materials. Methods CHS was purified by recrystallization, the pure CHS structure was characterizate by differential scanning calorimetry (DSC) and X ray diffraction (XRD), the free cholesterol content of the pure and crude were determinated by high performance liquid chromatography (HPLC), the degree of substitution of the cholesteryl was determinated before and after the purified by ¹H-NMR. Result CHS was purer after recrystallization, with property of crystal, which could significantly improve the degree of substitution of the cholesteryl. Conclusion The method of purification was simple and feasible, which was the basis of improving hydrophobically modified Bletilla striata polysaccharide's degree of substitution of the cholesteryl.
- the purity of raw materials /
- cholesteryl succinyl ester /
- substitution degree

[1]	孙达锋,史劲松,张卫明,等.白及多糖胶研究进展[J]. 食品科学,2009,30(3):296-298.
[2]	Zhang MS, Sun L, Zhao WC,et al.Cholesteryl-modification of a glucomannan from Bletilla striata and its hydrogel properties[J]. Molecules,2014,19(7):9089-9100.
[3]	刘晨光,董学猛,金晓明,等.基于疏水化修饰水溶性多糖的纳米粒制备及应用研究进展[J]. 功能材料,2007, 38(A05):1941-1946.
[4]	毕亚静,王艳萍,刘福强,等.胆甾醇琥珀酰基白及多糖的制备及其理化性质研究[J]. 药学实践杂志,2013,31(3):220-223.
[5]	杨文智.疏水改性普鲁兰多糖及其自组装载药纳米粒的研究. 北京:中国协和医科大学, 2009.
[6]	李磊.胆固醇基-羧甲基可德兰衍生物自聚集纳米粒子的制备以及作为抗肿瘤药物载体的研究. 北京:中国协和医科大学, 2010.
[7]	Na K,Park KH,Kim SW,et al. Self-assembled hydrogel nanoparticles from curdlan derivatives: characterization, anti-cancer drug release and interaction with a hepatoma cell line(HepG2)[J]. J Control Release,2000,69: 225-236.
[8]	李霞,刘晨光,贺爱华.L-丙交酯的纯化研究[J]. 青岛科技大学学报(自然科学版),2011,32(5):510-513.
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原料纯度对白及多糖疏水改性反应取代度的影响

doi: 10.3969/j.issn.1006-0111.2015.03.008

1. 延边大学药学院, 吉林延吉 133000;解放军208医院, 吉林长春 130062

2. 沈阳军区长春干休所, 吉林长春 130000

3. 解放军208医院, 吉林长春 130062

基金项目: 国家自然科学基金项目(81173559)

收稿日期: 2014-12-29
修回日期: 2015-01-20

关键词:

原料纯度 /

胆甾醇琥珀酸酯 /

取代度

摘要: 目的通过纯化反应原料之一胆甾醇琥珀酸酯(CHS),提高疏水改性白及多糖的胆甾醇基取代度。方法利用重结晶法纯化CHS,并采用差示量热扫描法(DSC)及X射线衍射法(XRD)对CHS纯品进行表征;用高效液相色谱法(HPLC)测定纯化前后产品中游离胆甾醇的含量;¹H-NMR法测定CHS纯化前后改性白及多糖的胆甾醇基取代度。结果重结晶后的CHS纯度较高,具有晶体性质,可明显提高改性白及多糖的胆甾醇基取代度。结论纯化方法简便、可行,为提高改性白及多糖胆甾醇基取代度提供研究基础。

English Abstract

Effect of raw material purity on the degree of substitution of hydrophobically modified Bletilla striata polysaccharide

1. School of Pharmacy, Yanbian University, Yanji 133000, China;No. 208 Hospital of PLA, Changchun 130062, China

2. Changchun Sanatorium for Retired Cadres, Shenyang Millitary Region, Changchun 130000, China

3. No. 208 Hospital of PLA, Changchun 130062, China

Received Date: 2014-12-29
Rev Recd Date: 2015-01-20

Keywords:

Abstract: Objective To improve the degree of substitution of the cholesteryl by purifying cholesteryl succinyl ester(CHS), one of the raw materials. Methods CHS was purified by recrystallization, the pure CHS structure was characterizate by differential scanning calorimetry (DSC) and X ray diffraction (XRD), the free cholesterol content of the pure and crude were determinated by high performance liquid chromatography (HPLC), the degree of substitution of the cholesteryl was determinated before and after the purified by ¹H-NMR. Result CHS was purer after recrystallization, with property of crystal, which could significantly improve the degree of substitution of the cholesteryl. Conclusion The method of purification was simple and feasible, which was the basis of improving hydrophobically modified Bletilla striata polysaccharide's degree of substitution of the cholesteryl.

引用本文:

Citation:

ZHAO Xuezhu, LIU Yang, WANG Yanping, LIU Fuqiang, ZHANG Qi, LIU Dan, ZHAO Nan, HAN Dan, ZHAO Qinglan. Effect of raw material purity on the degree of substitution of hydrophobically modified Bletilla striata polysaccharide[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(3): 221-225. doi: 10.3969/j.issn.1006-0111.2015.03.008

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原料纯度对白及多糖疏水改性反应取代度的影响

doi: 10.3969/j.issn.1006-0111.2015.03.008

Effect of raw material purity on the degree of substitution of hydrophobically modified Bletilla striata polysaccharide

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原料纯度对白及多糖疏水改性反应取代度的影响

doi: 10.3969/j.issn.1006-0111.2015.03.008

1. 延边大学药学院, 吉林 延吉 133000;解放军208医院, 吉林 长春 130062 2. 沈阳军区长春干休所, 吉林 长春 130000 3. 解放军208医院, 吉林 长春 130062

English Abstract

Effect of raw material purity on the degree of substitution of hydrophobically modified Bletilla striata polysaccharide

1. School of Pharmacy, Yanbian University, Yanji 133000, China;No. 208 Hospital of PLA, Changchun 130062, China 2. Changchun Sanatorium for Retired Cadres, Shenyang Millitary Region, Changchun 130000, China 3. No. 208 Hospital of PLA, Changchun 130062, China

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目录

1. 延边大学药学院, 吉林延吉 133000;解放军208医院, 吉林长春 130062

2. 沈阳军区长春干休所, 吉林长春 130000

3. 解放军208医院, 吉林长春 130062

1. School of Pharmacy, Yanbian University, Yanji 133000, China;No. 208 Hospital of PLA, Changchun 130062, China

2. Changchun Sanatorium for Retired Cadres, Shenyang Millitary Region, Changchun 130000, China

3. No. 208 Hospital of PLA, Changchun 130062, China