Determination of three flavonoid glycosides in Thesium chinensis Turcz by HPLC method
-
摘要: 目的 建立HPLC法同时测定百蕊草中3个黄酮苷类化合物的含量。 方法 色谱柱为DIKMA C18柱（250 mm×4.6 mm，5 μm），流动相为乙腈-水（21∶79，V/V），用冰醋酸调节pH至4.61，流速：1.0 ml/min，柱温：25℃，检测波长：346 nm。结果3个黄酮苷化合物山奈酚-3-O-L-吡喃鼠李糖基（1→2）-β-D-吡喃葡萄糖苷（百蕊草素Ⅰ）、山奈酚-3-O-β-D-吡喃葡萄糖苷（紫云英苷）、山奈酚-3-O-L-吡喃鼠李糖基（1→2）-[6-O-乙酰基]-β-D-吡喃葡萄糖苷（6'-乙酰氧基百蕊草素Ⅰ）的进样浓度分别在125~200、2.5~40、5.0~80 μg/ml范围内线性关系良好（r=0.999 9），平均加样回收率分别为101.63%、99.82%和102.02%，RSD分别为1.72%、2.34%和1.94%（n=6）。 结论 该方法准确，稳定性、重现性好，可作为百蕊草药材及制剂的质控方法。Abstract: Objective To establish an assay method for three flavonoid glycosides:kaempferol-3-O-L-rhamnopyranosyl(1→2)-β-D-glucopyranoside,kaempferol-3-O-glucoside and kaemperol-3-O-α-L-rhamnopyranosyl(1→2)-[6-O-acetyl]-β-D-glucopyranoside in Thesium chinensis Turcz by HPLC. Methods Dikma C18 column (520 mm×4.6 mm,5 μm) was used with the mobile phase of acetonitrile-water (21:79,adjusted pH to 4.61 with acetic acid) at a flow rate of 1.0 ml/min.The detection wavelength was set at 346 nm,and the column temperature was 25℃. Results The linear range of those three flavonoid glycosides was 125-200 μg/ml,2.5-40 μg/ml and 5.0-80 μg/ml (r=0.999 9).The average recoveries (n=6) were 101.63%,99.82% and 102.02%.RSD was 1.72%,2.34% and 1.94%(n=6) respectively. Conclusion This method is simple and accurate.It can be used for the quality control of Thesium chinensis Turcz and related preparations.
-
Key words:
- Thesium chinensis Turcz /
- flavonoid glycosides /
- HPLC /
- content determination
-
[1] 国家中医药管理局《中华本草》编委会.中华本草[M].上海:上海科学技术出版社,2005. [2] PARVEEN Z,DENG Y,SAEED MK,et al. Antiinflammatory and analgesic activities of Thesium chinense Turcz extracts and its major flavonoids,kaempferol and kaempferol-3-O-glucoside[J].Yakugaku Zasshi,2007,127(8):1275-1279. [3] 宣伟东,唐大海,卞俊,等.百蕊草对阿霉素肾病大鼠治疗作用的实验研究[J].药学实践杂志,2012,30(6):443-446. [4] 宣伟东,唐大海,范正平.百瑞口服液对阿霉素肾病大鼠综合症的治疗作用[J].中国药业,2014,23(9):8-10. [5] 闫俊,唐大海,宣伟东,等.百蕊草提取物对免疫球蛋白A肾病模型大鼠的治疗作用[J].药学实践杂志,2015,33(1):28-31,35. [6] 宣伟东,范正平,胡水根,等.百蕊草亲水性化学成分研究[J].药学实践杂志,2018,36(3):270-273. [7] 刘洋,邓玉林,禹玉洪,等.RP-HPLC测定百蕊草中紫云英苷的含量[J].中国中药杂志,2006,31(21):1766-1767. [8] 唐慧文,宋晓宁,骆红梅.HPLC法测定百蕊草中山奈酚的含量[J].贵阳中医学院学报,2006,28(4):60-61. [9] 储晓琴,蒋建勤,徐江慧,等.一测多评法测定百蕊草中百蕊草素Ⅰ、百蕊草素Ⅱ和山奈酚的含量[J].云南中医学院学报,2016,39(2):30-33. -
点击查看大图
计量
- 文章访问数: 3573
- HTML全文浏览量: 481
- PDF下载量: 321
- 被引次数: 0
下载:
