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七氟醚麻醉对新生鼠学习与记忆的影响

孙继辉,  陆志俊,  辛怡纯,  于布为

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文章导航 > 药学实践与服务  > 2015 >  33(4): 341-346
孙继辉, 陆志俊, 辛怡纯, 于布为. 七氟醚麻醉对新生鼠学习与记忆的影响[J]. 药学实践与服务, 2015, 33(4): 341-346. doi: 10.3969/j.issn.1006-0111.2015.04.014
引用本文: 孙继辉, 陆志俊, 辛怡纯, 于布为. 七氟醚麻醉对新生鼠学习与记忆的影响[J]. 药学实践与服务, 2015, 33(4): 341-346. doi: 10.3969/j.issn.1006-0111.2015.04.014
shu
SUN Jihui, LU Zhijun, Xin Yichun, Yu Buwei. The effects of sevofluraneon learning and memory of neonatal mice[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(4): 341-346. doi: 10.3969/j.issn.1006-0111.2015.04.014
Citation: SUN Jihui, LU Zhijun, Xin Yichun, Yu Buwei. The effects of sevofluraneon learning and memory of neonatal mice[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(4): 341-346. doi: 10.3969/j.issn.1006-0111.2015.04.014
shu

七氟醚麻醉对新生鼠学习与记忆的影响

doi: 10.3969/j.issn.1006-0111.2015.04.014
  • 1.

    同济大学医学院, 上海 200092

  • 2.

    上海交通大学医学院瑞金医院麻醉科, 上海 200025

  • 3.

    上海交通大学医学院瑞金医院卢湾分院麻醉科, 上海 200020

基金项目: 上海市自然科学课题基金项目(11ZR1423200)

The effects of sevofluraneon learning and memory of neonatal mice

  • 1.

    School of Medicine, Tongji University, Shanghai 200092, China

  • 2.

    Department of Anesthesia, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China

  • 3.

    Department of Anesthesia, Luwan Branch of Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200020, China

  • 摘要: 目的 探索用七氟醚麻醉发育期小鼠是否会引起成长过程中学习记忆障碍。 方法 实验包括122只新生鼠(出生后7 d)。其中72只分别经七氟醚1.0或0.5最低肺泡有效浓度(minimum alveolar concentration,MAC)麻醉或吸入40% O2 2 h,4周或12周行水迷宫实验,记录训练各天潜伏期和游泳速度,以及探索期平台滞留时间和平台穿越次数。另外50只小鼠用于测定七氟醚麻醉(1.0或0.5 MAC)过程(0、1、2 h)中动脉血血气分析。 结果 新生鼠在整个麻醉过程中,pH值、PaCO2、PaO2和SaO2均保持稳定,P>0.05。麻醉后4周,训练期后3 d,对照组小鼠潜伏期明显低于麻醉组,且后2 d,0.5 MAC组小鼠的潜伏期明显短于1.0 MAC组。探索期,对照组小鼠平台停留时间和平台穿越次数均明显高于2组麻醉组。麻醉后12周,1.0 MAC组小鼠在训练第5天潜伏期仍明显延长。探索期,对照组小鼠平台停留时间和(或)平台穿越次数均明显高于麻醉组。 结论 七氟醚麻醉引起新生鼠成长过程中学习与记忆障碍,其程度与药物浓度有关,且随时间推移减弱。
    Abstract: Objective To evaluate the effects of sevoflurane on learning and memory of neonatal mice. Methods 122 neonatalmice (7 days postnatal) were included in this study.72 of them were exposed to sevoflurane (1.0 or 0.5MAC,minimum alveolar concentration) or 40% O2 for 2 h(hours). Morris water maze was performed 4 and 12 weeks after anesthesia. Latency and swimming speed during training, time on island and times across island during the research were recorded. The rest 50 mice was used in artery blood analysis during sevoflurane (1.0 or 0.5 MAC, 0,1,2 h). Results pH,PaO2, PaCO2, SaO2 were stable during anesthesia. Latency in control group were significantly shorter than the two anesthesia groups 4 weeks after anesthesia and 3 days after the training. During the last 2 training days, the latency in 1.0 MAC sevoflurane-exposed mice were significantly longer than that of the 0.5 MAC group.12 weeks after anesthesia, the latency was still significantly longer in 1.0 MAC sevoflurane-exposed mice on the last training day. The time on island and/or times across island were significantly decreased in anesthesia groupsduring theresearch performed 4 weeks and 12 weeks after anesthesia. Conclusion Early exposure to sevoflurane leads to a concentration and time-depended persistent learning and memory deficits to neonatal mice.
  • [1] Kalkman CJ, Peelen L, Moons KG, et al. Behavior and development in children and age at the time of first anesthetic exposure[J]. Anesthesiology, 2009, 110(4): 805-812.
    [2] Wilder RT, Flick RP, Sprung J, et al. Early exposure to anesthesia and learning disabilities in a population-based birth cohort[J]. Anesthesiology, 2009, 110(4): 796-804.
    [3] Wise-Faberowski L, Zhang H, Ing R, et al.Isoflurane-induced neuronal degeneration: an evaluation in organotypic hippocampal slice culture [J].Anesth Analg, 2005, 101(3): 651-657.
    [4] Edwards DA, Shah HP, Cao W, et al. Bumetanide alleviates epileptogenic and neurotoxic effects of sevoflurane in neonatal rat brain[J]. Anesthesiology,2010,112(3): 567-575.
    [5] Kodama M, Satoh Y, Otsubo Y, et al. Neonatal desfluraneexposureinduces more robust neuroapoptosis than do isofluraneandsevoflurane and impairs working memory[J]. Anesthesiology,2011,115(5): 979-991.
    [6] Istaphanous GK, Howard J, Nan X, et al. Comparison of the neuroapoptoticproperties of equipotent anesthetic concentrationsofdesflurane, isoflurane, or sevoflurane in neonatal mice [J].Anesthesiology,2011, 114(3): 578-587.
    [7] 刘万里,薛 西,曹明芹,等.用SPSS实现完全随机设计多组比较秩和检验的多重比较[J].地方病通报, 2007,22(2): 27-29.
    [8] Bercker S, Bert B, BittigauP,etal.Neurodegeneration in newborn rats following propofolandsevoflurane anesthesia[J]. Neurotox Res,2009,16(2): 140-147.
    [9] Jevtovic-Todorovic V, Hartman RE, Izumi Y, et al. Earlyexposure to common anesthetic agents causes widespreadneurodegeneration in the developing rat brain and persistentlearning deficits[J]. J Neurosci,2003,23(3):876-882.
    [10] Liang G,Ward C, Peng J,et al. Isofluranecauses greater neurodegeneration than an equivalent exposureofsevoflurane in the developing brain of neonatal mice[J].Anesthesiology,2010,112(6): 1325-1334.
    [11] Loepke AW, McCann JC, Kurth CD, et al. The physiologiceffects of isoflurane anesthesia in neonatal mice[J].Anesth Analg, 2006, 102(1): 75-80.
    [12] Brambrink AM, Evers AS, Avidan MS, et al.Isoflurane-inducedneuroapoptosis in the neonatal rhesus macaquebrain[J]. Anesthesiology,2010, 112(4): 834-841.
    [13] Stratmann G, May LD, Sall JW, et al. Effect of hypercarbiaandisoflurane on brain cell death and neurocognitive dysfunctionin 7-day old rats[J]. Anesthesiology, 2009, 110(4): 849-861.
    [14] Vorhee CV, Williams MT. Morris water maze: procedures for assessing spatial and related forms of learning and memory[J]. Nat Prot,2006, 1(2): 848-858.
    [15] Zhou X, Song FH, He W, et al. Neonatal exposure to sevoflurane causes apoptosis and reduces nNOS protein expression in rat hippocampus[J]. Mol Med Rep, 2012, 6(3): 543-546.
    [16] Ramage TM, Chang FL, Shih J, et al. Distinct long-term neurocognitive outcomes after equipotent sevoflurane or isofluraneanaesthesia in immature rats[J]. Br J Anaesth, 2013, 110 (S1): i39-i46.
    [17] Shelton KL.Discriminative stimulus effects of inhaled 1,1,1-trichloroethane in mice: comparison to other hydrocarbon vapors and volatile anesthetics[J]. Psychopharmacology (Berl),2009,203(2): 431-440.
    [18] Nishikawa K, Harrison NL.The actions of sevoflurane and desflurane on the gamma-aminobutyric acid receptor type A: effects of TM2 mutations in the alpha and betasubunits[J]. Anesthesiology,2003,99(3): 678-684.
    [19] Daval JL, Vert P. Apoptosis and neurogenesis after transienthypoxia in the developing rat brain[J]. Semin Perinatol,2004,28(4):257-263.
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  • 收稿日期:  2014-06-04
  • 修回日期:  2014-10-27

七氟醚麻醉对新生鼠学习与记忆的影响

doi: 10.3969/j.issn.1006-0111.2015.04.014
  • 1. 同济大学医学院, 上海 200092
  • 2. 上海交通大学医学院瑞金医院麻醉科, 上海 200025
  • 3. 上海交通大学医学院瑞金医院卢湾分院麻醉科, 上海 200020
    • 基金项目:  上海市自然科学课题基金项目(11ZR1423200)
  • 收稿日期: 2014-06-04
  • 修回日期: 2014-10-27

摘要: 目的 探索用七氟醚麻醉发育期小鼠是否会引起成长过程中学习记忆障碍。 方法 实验包括122只新生鼠(出生后7 d)。其中72只分别经七氟醚1.0或0.5最低肺泡有效浓度(minimum alveolar concentration,MAC)麻醉或吸入40% O2 2 h,4周或12周行水迷宫实验,记录训练各天潜伏期和游泳速度,以及探索期平台滞留时间和平台穿越次数。另外50只小鼠用于测定七氟醚麻醉(1.0或0.5 MAC)过程(0、1、2 h)中动脉血血气分析。 结果 新生鼠在整个麻醉过程中,pH值、PaCO2、PaO2和SaO2均保持稳定,P>0.05。麻醉后4周,训练期后3 d,对照组小鼠潜伏期明显低于麻醉组,且后2 d,0.5 MAC组小鼠的潜伏期明显短于1.0 MAC组。探索期,对照组小鼠平台停留时间和平台穿越次数均明显高于2组麻醉组。麻醉后12周,1.0 MAC组小鼠在训练第5天潜伏期仍明显延长。探索期,对照组小鼠平台停留时间和(或)平台穿越次数均明显高于麻醉组。 结论 七氟醚麻醉引起新生鼠成长过程中学习与记忆障碍,其程度与药物浓度有关,且随时间推移减弱。

English Abstract

孙继辉, 陆志俊, 辛怡纯, 于布为. 七氟醚麻醉对新生鼠学习与记忆的影响[J]. 药学实践与服务, 2015, 33(4): 341-346. doi: 10.3969/j.issn.1006-0111.2015.04.014
引用本文: 孙继辉, 陆志俊, 辛怡纯, 于布为. 七氟醚麻醉对新生鼠学习与记忆的影响[J]. 药学实践与服务, 2015, 33(4): 341-346. doi: 10.3969/j.issn.1006-0111.2015.04.014
shu
SUN Jihui, LU Zhijun, Xin Yichun, Yu Buwei. The effects of sevofluraneon learning and memory of neonatal mice[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(4): 341-346. doi: 10.3969/j.issn.1006-0111.2015.04.014
Citation: SUN Jihui, LU Zhijun, Xin Yichun, Yu Buwei. The effects of sevofluraneon learning and memory of neonatal mice[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(4): 341-346. doi: 10.3969/j.issn.1006-0111.2015.04.014
shu

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