Determination of ambroxol hydrochloride in humam plasma by HPLC
-
摘要: 目的 建立HPLC测定人血浆中盐酸氨溴索含量的方法。 方法 选用Diamonsil-C18柱(200 mm×4.6 mm,5μm),流动相:甲醇-0.01 mol/L磷酸盐缓冲液(pH 7.0)-四氢呋喃(700:320:25,v/v/v),流速:1.2 ml/min,检测波长:242 nm,柱温:室温。 结果 该方法低、中、高3种浓度的提取回收率为88.2％ ~104.8％ ,日内、日间相对标准差为2.7％~5.3％(n=6),在10.2~610.8 ng/ml范Abstract: Objective To develop a fast reverse-phase high-performance liquid chromatography assay method for the determination of ambroxol hydrochloride. Methods A Diamonsil-C18(200 mm×4.6 mm,5μm)column was used.The mobile phase consisted of methanol-0.01 mol/L PBS(pH 7.0)-THF(700:320:25,v/v/v).Detection wavelength was 242 nm. Results A good linear relationship was obtained in the concentration range of 10.2~610.8 ng/ml. The average recovery of this method was 88.2％~104.8％. The RSD of within-day and between-day were all less than 15％. Conclusion The method was simple,rapid and accurate. The result showed that the method was suitable for the determination of ambroxol hydrochloride.
-
Key words:
- Ambroxol hydrochloride /
- HPLC /
- Plasma concentration
-
[1] 国家药典委员会. 中华人民共和国药典临床用药须知:化学药和生物制品卷[M]. 北京:人民卫生出版社,2005:242. [2] NobilisM,Pastera J,SvobodaD,et al.High-performance liquid chromatography determination of ambroxol in human plasma[J].J Chromatogr,1992,581:251. [3] 钟大放. 以加权最小二乘法建立生物分析标准曲线的若干问题[J] . 药物分析杂志,1996,16(5):343. [4] 戈升荣,安富荣,曹惠明,等. 高效液相色谱法测定人血浆中盐酸氨溴索浓度[J]. 中国药房,2004,15(1):37. -
点击查看大图
计量
- 文章访问数: 6764
- HTML全文浏览量: 345
- PDF下载量: 404
- 被引次数: 0
下载:
